Methods of improving seed size and quality

ABSTRACT

The invention relates to a method of increasing seed size and/or seed quality in a plant, the method comprising increasing the permease activity of an amino acid permease (AAP). The invention also relates to method of making such plants as well as plants that display an increased seed size and/or seed quality.

FIELD OF THE INVENTION

The invention relates to a method of increasing seed yield in a plant,the method comprising increasing the permease activity of an amino acidpermease (AAP). The invention also relates to a method of making suchplants as well as plants that display an increase in seed yield.

BACKGROUND OF THE INVENTION

Seed size and weight are associated with seed yield, thereby determiningseed production in crops. Seed size is also recognized as a criticalfactor for evolutionary adaption. Seedlings from large seeds have beenproposed to possess stronger ability to survive under stress conditions,while plant species with small seeds have been suggested to have abetter ability to propagate progeny. A mature seed contains the maternalinteguments, the endosperm and the embryo. The complex interactionsbetween the maternal tissues, the endosperm and the embryo regulate seedgrowth and determine seed size and weight in plants.

The analysis of seed mutants has identified several important regulatorsof seed size in Arabidopsis. Several of these regulators have beenreported to regulate seed size by influencing cell proliferation inmaternal tissues, such as KLU/CYTOCHROME P450 78A5 (CYP78A5),ubiquitin-dependent protease DA1, E3 ubiquitin ligases BIG BROTHER (BB)and DA2, transcription factors AUXIN RESPONSE FACTOR 2 (ARF2) and NGAL2,and UBIQUITIN SPECIFIC PROTEASE 15 (UBP15). By contrast, transcriptionfactors TESTA GLABRA 2 (TTG2) and APETALA2 (AP2) may act maternally toregulate seed size by influencing cell expansion. The development ofzygotic tissues also affects seed growth. MINISEED3 (MINI3) and HAIKU(IKU) regulate endosperm cellularization, thereby influencing seed size.SHORT HYPOCOTYL UNDER BLUE1 (SHB1) can bind to the promoters of IKU2 andMINI3 and promotes their expression.

Seed size is often controlled by quantitative trait loci (QTLs)(Alonso-Blanco et al., 1999; Song et al., 2007). In Arabidopsis, severalquantitative trait loci (QTLs) for seed size have been mapped, but thegenes corresponding to these QTLs have not been cloned so far. Seedquality, and in particular, free amino acid and protein content is animportant contributor to seed yield. Increasing grain protein levels hassignificant value when growing grain crops for animal feed or for use inhuman consumption (such as bread-making or brewing) However, developinghigh quality seeds is precluded by the inverse relationship between seedquality (in particular protein content) and size.

The present invention addresses the need to enhance seed size andimprove seed quality of commercially value crops, such as wheat, riceand maize, for example.

SUMMARY OF THE INVENTION

Here we report a major QTL gene for seed size and weight on chromosome 1(SSW1) in Arabidopsis, which encodes an amino acid permease (AAP),specifically AtAAP8. Amino acids are an important source of organicnitrogen in most plant species, and the delivery of nitrogen to sinks iscrucial for seed development.

Our findings identify the first gene corresponding to the QTL for seedsize, weight and quality (SSW1/AAP8) in Arabidopsis and demonstrate thatnatural allelic variation in SSW1/AAP8 contributes to the amino acidtransport activity of SSW1/AAP8, thereby regulating seed size, weightand quality. In particular, Arabidopsis accessions possess three typesof natural allelic variation in the SSW1/AAP8 gene, includingSSW1^(Cvi), SSW1^(Ler) and SSW1^(Col-0) types. The SSW1^(Cvi) alleleproduces larger and heavier seeds with more free amino acids and storageproteins than the SSW1^(Ler) allele. SSW1^(Cvi) has similar amino acidtransport activity to SSW1^(Col-0) and possesses higher amino acidtransport activity than SSW1^(Ler). We have further found that naturalvariation in the amino acid (A410V) is predominantly responsible for theobserved differences in the amino acid transport activity of the SSW1types. We have also found that loss of function of SSW1/AAP8 causessmall and light seeds.

Our results reveal that AAP8 is an important molecular and genetic basisfor natural variation in seed size, weight and quality control, and showthat this gene is an important target to improve both seed weight andquality in plants.

Accordingly, in a first aspect of the invention, there is provided amethod of increasing seed yield in a plant, the method comprisingincreasing the activity of amino acid permease (AAP). Preferably, anincrease in seed yield comprises an increase in seed size and/or seedquality, preferably an increase in seed size and quality.

In one embodiment, the method comprises increasing the expression ofAAP8, wherein the amino acid sequence of AAP8 comprises a sequence asdefined in SEQ ID NO: 2, 3 or 4 or a functional variant or homologuethereof. Most preferably, the amino acid sequence of AAP8 comprises SEQID NO: 4 or a functional variant or homologue thereof.

In one embodiment, the method comprises introducing and expressing anucleic acid construct, wherein the construct comprises a nucleicsequence encoding an AAP8 polypeptide as defined in SEQ ID NO: 2, 3 or 4or a functional variant or homologue thereof. Preferably, the nucleicacid sequence is operably linked to a regulatory sequence. Morepreferably, the regulatory sequence is a constitutive or tissue-specificpromoter, such as the MUM4 promoter.

In an alternative embodiment, the method comprises introducing at leastone mutation into the plant genome, wherein said mutation increases theactivity of an AAP polypeptide. Preferably, the mutation is introducedusing targeted genome editing. More preferably, the targeted genomeediting is CRISPR.

In one embodiment, the mutation is the insertion of at least oneadditional copy of a nucleic acid sequence encoding an AAP8 polypeptideor a homolog or functional variant thereof, such that the nucleic acidsequence is operably linked to a regulatory sequence, and wherein themutation is introduced using targeted genome editing and whereinpreferably the nucleic acid sequence encodes an AAP polypeptide asdefined in SEQ ID NO: 2, 3 or 4 or a functional variant or homologthereof.

In an alternative embodiment, the method comprises or results inintroducing at least one mutation at position 410 of SEQ ID NO: 1 or ata homologous position in a homologous sequence. Preferably, the mutationis a substitution.

In another aspect of the invention, there is provided a geneticallyaltered plant, part thereof or plant product, wherein the plant ischaracterised by an increase in seed yield.

Preferably, the genetically altered plant, part thereof or plant producthas increased activity of an AAP polypeptide.

In one embodiment, the plant expresses a nucleic acid constructcomprising a nucleic acid encoding an AAP8 polypeptide as defined in anyof SEQ ID NO: 2, 3 or 4 or a functional variant or homologue thereof.

In an alternative embodiment, the plant has at least one mutation in itsgenome, wherein the mutation increases the activity of AAP8. Preferably,the mutation is introduced by targeted genome editing, preferablyCRISPR.

In one embodiment, the mutation is the insertion of at least one or moreadditional copy of a nucleic acid encoding an AAP8 polypeptide asdefined in SEQ ID NO: 2, 3 or 4 or homolog or functional variantthereof. Alternatively, the mutation is at position 410 of SEQ ID NO: 1or at a homologous position in a homologous sequence.

In another aspect of the invention, there is provided a method of makinga transgenic plant having an increase in seed yield, the methodcomprising introducing and expressing a nucleic acid constructcomprising a nucleic acid sequence encoding an AAP8 polypeptide asdefined in SEQ ID NO: 2, 3 or 4 or a functional variant or homologthereof.

In a further aspect of the invention, there is provided a method ofmaking a genetically altered plant having an increase in seed yield, themethod comprising introducing a mutation into the plant genome toincrease the activity of an AAP8 polypeptide. Preferably, the mutationis introduced using targeted genome editing, preferably CRISPR.

In one embodiment, the mutation is the insertion of one or moreadditional copies of a nucleic acid encoding an AAP8 polypeptide asdefined in SEQ ID NO: 2, 3 or 4 or a functional variant or homologthereof, such that the sequence is operably linked to a regulatorysequence. In an alternative embodiment, the method comprises or resultsin introducing at least one mutation at position 410 of SEQ ID NO: 1 orat a homologous position in a homologous sequence. Preferably, themutation is a substitution.

In a further aspect of the invention, there is provided a method ofscreening a population of plants and identifying and/or selecting aplant that has or will have increased activity of a AAP polypeptide, themethod comprising detecting in the plant germplasm at least onepolymorphism in the nucleic acid encoding an AAP polypeptide ordetecting at least one polymorphism in an AAP protein and selecting saidplant or progeny thereof.

In one embodiment, the polymorphism is a substitution. Preferably, thesubstitution is at position 410 of SEQ ID NO: 1, 2, 3 or 4 or position2635 of SEQ ID NO: 5, 6, 7 or 8 or a homologous substitution in ahomologous sequence.

In one embodiment, a “homologous substitution in a homologous sequence”in any of the aspects of the invention described herein, may be selectedfrom one or more of the positions in one of the homologous sequencesdefined in Table 12.

In a further aspect of the invention there is provided a nucleic acidconstruct comprising a nucleic acid sequence encoding a AAP8 polypeptideas defined in SEQ ID NO: 2, 3 or 4 or a functional variant or homologthereof. More preferably, the nucleic acid sequence is operably linkedto a regulatory sequence, wherein the regulatory sequence is selectedfrom a constitutive promoter or a tissue-specific promoter.

Also provided is a vector comprising the nucleic acid constructdescribed above, as well as a host cell comprising the nucleic acidconstruct.

In another aspect of the invention, there is provided the use of thenucleic acid construct or vector described above to increase seed yield.

In a final aspect of the invention there is provided a method ofproducing a food or feed composition, the method comprising

-   -   a. producing a plant wherein the activity of an AAP polypeptide        is increased using the method described above;    -   b. obtaining a seed from said plant; and    -   c. producing a food or feed composition from said seed.

In one embodiment, the plant is a crop plant. In a further embodiment,the crop plant is selected from rice, maize, wheat, soybean, barley,cannabis, pennycress and Brassica. In a preferred embodiment, the plantpart is a seed.

In a further aspect of the invention, there is provided a plant or plantprogeny obtained or obtainable by any of the methods described above. Inanother embodiment, there is provided a seed obtained or obtainable bythe plants or methods described herein, as well as progeny obtained fromthose plants and subsequent seeds obtained from the plants.

In a further aspect of the invention, there is provided a method ofincreasing free amino acid and/or protein content in a plant comprisingincreasing the activity of amino acid permease (AAP). Preferably, freeamino acid and/or protein content is increased in the seed or grain ofsaid plant. In one embodiment, the method comprises increasing theexpression and/or activity of AAP8, wherein the amino acid sequence ofAAP8 comprises a sequence as defined in SEQ ID NO: 2, 3 or 4 or afunctional variant or homologue thereof.

DESCRIPTION OF THE FIGURES

The invention is further described in the following non-limitingfigures:

FIG. 1 shows that the NIL-SSW1^(Cvi) produces large seeds. (A) Matureseeds of Ler (left) and NIL-SSW1^(Cvi) (right). (B) Mature embryos ofLer (left) and NIL-SSW1^(Cvi) (right). (C) and (D) Ten-day-old seedlingsof Ler (C) and NIL-SSW1^(Cvi) (D). (E) and (F) The average area of Lerand NIL-SSW1^(Cvi) seeds from main stems (E) and branches (F). (G) to(I) Length, width and weight of Ler and NIL-SSW1^(Cvi) seeds from mainstems.

(J) The average cotyledon area of 10-d-old seedlings of Ler andNIL-SSW1^(Cvi). Values in (E) to (J) are given as mean±SE relative tothe wild-type values, set at 100%. **, P<0.01 compared with the wildtype (Student's t test). Bars=0.5 mm in (A), 0.1 mm in (B), 1 mm in (C)and (D).

FIG. 2 shows that SSW1 regulates cell proliferation in the maternalinteguments.

(A) Seed area of Ler/Ler F₁, SSW1^(Cvi)/SSW1^(Cvi) F₁, Ler/SSW1^(Cvi) F₁and SSW1^(Cvi)/Ler F₁. (B) Seed area of Ler/Ler F₂,SSW1^(Cvi)/SSW1^(Cvi) F₂, Ler/SSW1^(Cvi) F₂ and SSW1^(Cvi)/Ler F₂. (C)and (D) The mature ovules of Ler (C) and SSW1^(Cvi) (D). (E) and (F) Theseeds of Ler (E) and SSW1^(Cvi) (F) at 6 DAP (days after pollination).(G) The outer integument length of Ler and SSW1^(Cvi) at 0, 6, 8 DAP.(H) The number of cells in the outer integuments of Ler and SSW1^(Cvi)at 0, 6, 8 DAP. (I) The length of cells in the outer integuments of Lerand SSW1^(Cvi) at 0, 6, 8 DAP. Values in (A) and (B) are given asmean±SE relative to respective wildtype values, set at 100%. Values in(G) to (I) are given as mean±SE. **, P<0.01 compared with the wildtypeby Student's t test. Bar=100 μm in (C) to (F).

FIG. 3 shows that the SSW1/AAP8 gene encodes the amino acid permease 8(AAP8). (A) and (B) The AAP8 gene was mapped into the interval betweenmarkers Cvi-m33 and Cvi-m51 by using an F₂ population of 10,048individuals and progeny tests. The mapping region contains four genes.(C) Quantitative real-time PCR analysis show expression of At1g10010,At1g10020, At1g10030 and At1g10040 in the 2nd to 5th siliques from Lerand NIL-SSW1^(Cvi) main stems. (D) The structure of the SSW1/AAP8 gene.The red color marked substitutions can cause amino acid change.

(E) Distribution of Arabidopsis accessions with SSW1^(Ler), SSW1^(Cvi)and SSW1^(Col-0) types, respectively. (F) The schematic diagram of theSSW1/AAP8 protein. Amino acid substitutions are marked asLer/SSW1^(Cvi). For example, A/V means alanine in Ler and valine in Cviand NIL-SSW1^(Cvi). “Aa_trans motif” represents “amino acid transporter”in Pfam database (PF01490). (G) Seed area and weight of Ler,NIL-SSW1^(Cvi), gSSW1^(Cvi)-COM #6 (homozygous), gSSW1^(Cvi)-COM #9(homozygous) and gSSW1^(Cvi)-COM #16 (homozygous). (H) The expressionlevels of AAP8 in Col-0, aap8-1, and aap8-101.

(I) Seed area and weight of Col-0, aap8-1, and aap8-101. (J) Seed areaof Col-0, aap8-1, gSSW1^(Cvi)-COM; aap8-1 #1 (homozygous),gSSW1^(Cvi)-COM; aap8-1 #2 (homozygous) and gSSW1^(Cvi)-COM; aap8-1 #3(homozygous). Values in (C) and (H) are given as mean±SE. Values in (G)(I) and (J) are given as mean±SE relative to the respective wild-typevalues, set at 100%. **, P<0.01 compared with the wild-type (Student's ttest).

FIG. 4 shows that natural variation in SSW1/AAP8 influences amino acidpermease activity. (A) Schematic representation of SSW1 harboringdifferent natural allelic variations and mutations. Three types ofnatural allelic variations in SSW1/AAP8 (SSW1^(Ler), SSW1^(Cvi), andSSW1^(Col-0)) were shown. (B) Growth of 22Δ8AA transformed with SSW1harboring different amino acid variations or mutations in nitrogen freemedium supplemented with 1 mM ASP.

Values in (B) are given as mean±SE.

FIG. 5 shows that the SSW1^(Cvi) natural allele seeds contain more freeamino acids and storage proteins. (A) Comparison of free amino acidcontent of young siliques (2-5 days after pollination) of Ler andNIL-SSW1^(Cvi). (B) Comparison of free amino acid content of dry seedsof Ler and NIL-SSW1^(Cvi). (C) Analysis of total free amino acid contentof young siliques (2-5 days after pollination, left) and dry seeds(right) of Ler and NIL-SSW1^(Cvi). (D) Analysis of soluble seed proteinsby SDS-PAGE gel.

Values in (A) and (B) are given as mean±SE. Values in (C) is given asmean±SE relative to the respective wild-type values, set at 100%. **,P<0.01 and *, P<0.05 compared to the wildtype by Student's t test. (E)Quantification of the soluble seed proteins in Ler was relative to thatin NIL-SSW1^(Cvi) from (D). The ratio values of soluble seed proteins inLer were set at 1. Values for soluble seed proteins in NIL-SSW1^(Cvi)are given as mean±SD (n=3). **P<0.01 compared with the value for Ler byStudent's t-test. Values in (A) and (B) are given as mean±SE. Values in(C) and (E) is given as mean±SE relative to the respective wild-typevalues, set at 100%. **, P<0.01 and *, P<0.05 compared to the wildtypeby Student's t test.

FIG. 6 shows the genetic interactions between AAP8/SSW1 and AAP1. (A)The AAP1 gene structure. The T-DNA insertion site in aap1-101 was shown.Arrows indicate the priming site of primes used for Real-time PCR in(C). (B) The AAP1 protein structure. (C) The expression levels of AAP1in Col-0 and aap1-101. (D) Seed area of Col-0, aap8-1, aap1-101, andaap8-1 aap1-101. (E) Seed weight of Col-0, aap8-1, aap1-101, and aap8-1aap1-101. (F) A model for AAP8 regulation in amino acid permeaseactivity between different natural allelic variations/two Arabidopsisaccessions. This includes transporters involved in amino acid uptakeinto the endosperm (AAP8/SSW1) and embryo (AAP1). Different arrow shapesrepresent that amino acids are transported by different transporters(SSW1/AAP8 and AAP1). Thicker arrows represent higher amino acidpermease activity. The amino acid V410A is mainly responsible for theactivity differences between SSW1^(Cvi) and SSW1^(Ler). Values in (D) to(E) are given as mean±SE relative to the respective wild-type values,set at 100%. **, P<0.01 compared with their respective control(Student's t test).

FIG. 7 shows the seed area and weight of Ler, LCN1-3-3 and Cvi. Valuesare given as mean±SE relative to Ler, set at 100%.

FIG. 8 shows the seed area of gSSW1Ler-COM #and gSSW1^(Cvi)-COM#transgenic lines. Values are given as mean±SE relative to therespective wild-type values, set at 100%. **, P<0.01 compared with thewild-type (Student's t test).

FIG. 9 shows that the seed size of aap8-1 is controlled maternally. (A)Seed area of Col-0/Col-0 F1, aap8-1/aap8-1 F1, Col-0/aap8-1 F1 andaap8-1/Col-0 F1. (B) Seed area of Col-0/Col-0 F2, aap8-1/aap8-1 F2,Col-0/aap8-1 F2 and aap8-1/Col-0 F2. (C) The outer integument length ofCol-0 and aap8-1 at 0, 6, 8 DAP. (D) The number of cells in the outerinteguments of Col-0 and aap8-1 at 0, 6, 8 DAP. (E) The length of cellsin the outer integuments of Col-0 and aap8-1 at 0, 6, 8 DAP. Values in(A) and (B) are given as mean±SE relative to the respective wild-typevalues, set at 100%. Values in (C) to (E) are given as mean±SE. **,P<0.01 compared with the wild-type (Student's t test).

FIG. 10 shows the gSSW1Cvi-COM #transgene lines contain more storageproteins. (a) The contents of soluble seed proteins by SDS-PAGE of threedifferent gSSW1Cvi-COM lines (homozygous) and their individual Lercounterparts. We obtained Ler #1 (Lane A) and gSSW1Cvi-COM #9 (Lane B)seeds, Ler #2 (Lane C) and gSSW1Cvi-COM #5 (Lane D) seeds, Ler #3 (LaneE) and gSSW1Cvi-COM #15 (Lane F) seeds from their respectiveheterozygous maternal lines. (b) Quantification of the soluble seedproteins in different gSSW1^(Cvi)-COM transgene lines was relative tothat in Ler from (A) and Supplemental FIG. 14B. The ratio values ofsoluble seed proteins in Ler were set at 1. Values for soluble seedproteins in gSSW1^(Cvi)-COM are given as mean±SD (n=3). **P<0.01compared with the value for Ler by Student's t-test.

FIG. 11 is a list of SNPs in the SSW1 gene between Ler and Cvi.

FIG. 12 shows a table of point mutations at the homologous sequenceposition to At AAP8 A410. Homologous species listed are Rice, Maize,Barley, Soy Bean, Wheat and Brassica.

DETAILED DESCRIPTION OF THE INVENTION

The present invention will now be further described. In the followingpassages, different aspects of the invention are defined in more detail.Each aspect so defined may be combined with any other aspect or aspectsunless clearly indicated to the contrary. In particular, any featureindicated as being preferred or advantageous may be combined with anyother feature or features indicated as being preferred or advantageous.

The practice of the present invention will employ, unless otherwiseindicated, conventional techniques of botany, microbiology, tissueculture, molecular biology, chemistry, biochemistry and recombinant DNAtechnology, bioinformatics which are within the skill of the art. Suchtechniques are explained fully in the literature.

The terms “seed” and “grain” as used herein can be used interchangeably.

As used herein, the words “nucleic acid”, “nucleic acid sequence”,“nucleotide”, “nucleic acid molecule” or “polynucleotide” are intendedto include DNA molecules (e.g., cDNA or genomic DNA), RNA molecules(e.g., mRNA), natural occurring, mutated, synthetic DNA or RNAmolecules, and analogs of the DNA or RNA generated using nucleotideanalogs. It can be single-stranded or double-stranded. Such nucleicacids or polynucleotides include, but are not limited to, codingsequences of structural genes, anti-sense sequences, and non-codingregulatory sequences that do not encode mRNAs or protein products. Theseterms also encompass a gene. The term “gene” or “gene sequence” is usedbroadly to refer to a DNA nucleic acid associated with a biologicalfunction. Thus, genes may include introns and exons as in the genomicsequence, or may comprise only a coding sequence as in cDNAs, and/or mayinclude cDNAs in combination with regulatory sequences.

The terms “polypeptide” and “protein” are used interchangeably hereinand refer to amino acids in a polymeric form of any length, linkedtogether by peptide bonds.

The aspects of the invention involve recombination DNA technology andexclude embodiments that are solely based on generating plants bytraditional breeding methods.

For the purposes of the invention, a “genetically altered” or “mutant”plant is a plant that has been genetically altered compared to thenaturally occurring wild type (WT) plant. In one embodiment, a mutantplant is a plant that has been altered compared to the naturallyoccurring wild type (WT) plant using a mutagenesis method, such as themutagenesis methods described herein. In one embodiment, the mutagenesismethod is targeted genome modification or genome editing. In oneembodiment, the plant genome has been altered compared to wild typesequences using a mutagenesis method. In one example, mutations can beused to insert an AAP gene sequence to increase the activity of AAP. Inone example, the AAP sequence is operably linked to an endogenouspromoter. Such plants have an altered phenotype as described herein,such as an increased seed yield. Therefore, in this example, increasedseed yield is conferred by the presence of an altered plant genome andis not conferred by the presence of transgenes expressed in the plant.

Methods of Increasing Seed Yield

In a first aspect of the invention, there is provided a method ofincreasing seed yield in a plant, the method comprising increasing theactivity of an amino acid permease (AAP) in a plant.

Seed size and weight are the main components contributing to seed yield,however, in one embodiment, the increase in seed yield comprises anincrease in at least one yield component trait such as seed length andseed width, including average seed length, width and/or area, seedweight (single seed or thousand grain weight), overall seed yield perplant, and/or seed quality (preferably an increase in storage proteinsand/or free amino acids) per seed. In particular, the inventors havefound that increasing the activity of an AAP increases at least one ofseed weight, seed size and seed quality. Preferably, increasing theactivity of an AAP increases seed weight, seed size and seed quality.

The terms “increase”, “improve” or “enhance” as used herein areinterchangeably. In one embodiment, seed yield, and preferably seedweight, seed size (e.g. seed length and/or width and/or seed area)and/or seed quality is increased by at least 2%, 3%, 4%, 5%, 6%, 7%, 8%,9%, 10% 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 30%, 40% or50% compared to a control plant. Preferably, seed yield is increased byat least 5%, more preferably between 5 and 30% compared to a controlplant. In one embodiment, total free amino acid content in the seedsincreased by between 5 and 50%, more preferably between 10 and 40%compared to a control plant.

Thus, according to the invention, seed yield can be measured byassessing one or more of seed weight, seed size and/or protein (or freeamino acid) content in the plant. Yield is increased relative to controlplants. The skilled person would be able to measure any of the aboveseed yield parameters using known techniques in the art. Protein oramino acid levels may be measured using standard techniques in the art,such as, but not limited to, infrared radiation analyses and use of theBradford assay.

Accordingly, in another aspect of the invention, there is provided amethod of increasing free amino acid and/or protein content in a plantcomprising increasing the activity of amino acid permease (AAP).Preferably, free amino acid and/or protein content is increased in theseed or grain of said plant.

Amino acid permease or AAP is a membrane transport protein thattransports amino acids into the cell. By “increase activity” is meantthat the ability of the permease to transport amino acids, an inparticular, aspartate, into a cell is increased, particularly whencompared to a wild-type or control plant. FIG. 4 shows one method tomeasure the activity of an amino acid permease, but other methods wouldbe well known to the skilled person.

In one embodiment, the AAP is AAP8 (which is also referred to herein asSSW1). More preferably AAP8 comprises or consists of an amino acidsequence as defined in any one of SEQ ID NO: 1 to 4 or a functionalvariant or homologue thereof. In a further preferred embodiment, AAP8comprises or consists of a nucleic acid sequence as defined in any oneof SEQ ID NO: 5 to 8 or a functional variant or homologue thereof.

In one embodiment, the activity of an AAP is increased by introducingand expressing a nucleic acid construct where the nucleic acid constructcomprises a nucleic acid sequence encoding an AAP8 polypeptide asdefined in SEQ ID NO: 2 (the Cvi allele) or 3 (the Col-0 allele) or 4 ora functional variant or homolog thereof. In a further embodiment, thenucleic acid construct comprises a nucleic acid sequence comprising orconsisting of a nucleic acid sequence as defined in SEQ ID NO: 6, 7 or 8or functional variant or homolog thereof.

In a preferred embodiment, the nucleic acid sequence is operably linkedto a regulatory sequence. Accordingly, in one embodiment, the nucleicacid sequence may be expressed using a regulatory sequence that drivesoverexpression. Overexpression according to the invention means that thetransgene is expressed or is expressed at a level that is higher thanthe expression of the endogenous AAP gene whose expression is driven byits endogenous counterpart. In one embodiment, the nucleic acid andregulatory sequence are from the same plant family. In anotherembodiment, the nucleic acid and regulatory sequence are from adifferent plant family, genus or species—for example, AtAAP8 isexpressed in a plant that is not Arabidopsis.

In one embodiment, the regulatory sequence is a promoter. The term“promoter” typically refers to a nucleic acid control sequence locatedupstream from the transcriptional start of a gene and which is involvedin the binding of RNA polymerase and other proteins, thereby directingtranscription of an operably linked nucleic acid. Encompassed by theaforementioned terms are transcriptional regulatory sequences derivedfrom a classical eukaryotic genomic gene (including the TATA box whichis required for accurate transcription initiation, with or without aCCAAT box sequence) and additional regulatory elements (i.e. upstreamactivating sequences, enhancers and silencers) which alter geneexpression in response to developmental and/or external stimuli, or in atissue-specific manner. Also included within the term is atranscriptional regulatory sequence of a classical prokaryotic gene, inwhich case it may include a −35 box sequence and/or −10 boxtranscriptional regulatory sequences.

A “plant promoter” comprises regulatory elements that mediate theexpression of a coding sequence segment in plant cells. The promotersupstream of the nucleotide sequences useful in the nucleic acidconstructs described herein can also be modified by one or morenucleotide substitution(s), insertion(s) and/or deletion(s) withoutinterfering with the functionality or activity of either the promoters,the open reading frame (ORF) or the 3′-regulatory region such asterminators or other 3′ regulatory regions which are located away fromthe ORF. It is furthermore possible that the activity of the promoter isincreased by modification of their sequence, or that they are replacedcompletely by more active promoters, even promoters from heterologousorganisms. For expression in plants, the AAP nucleic acid sequence is,as described above, preferably linked operably to or comprises asuitable promoter, which expresses the gene at the right point in timeand with the required spatial expression pattern.

In one embodiment, overexpression may be driven by a constitutivepromoter. A “constitutive promoter” refers to a promoter that istranscriptionally active during most, but not necessarily all, phases ofgrowth and development and under most environmental conditions, in atleast one cell, tissue or organ. Examples of constitutive promotersinclude the cauliflower mosaic virus promoter (CaMV35S or 19S), riceactin promoter, ubiquitin promoter, rubisco small subunit, maize oralfalfa H3 histone, OCS, SAD1 or 2, GOS2 or any promoter that givesenhanced expression

In an alternative embodiment, the promoter is a tissue-specificpromoter. Tissue specific promoters are transcriptional control elementsthat are only active in particular cells or tissues at specific timesduring plant development. In one example, the tissue-specific promoteris a seed coat-specific promoter, for example, the MUM4(Mucilage-modified4)0.3Pro, as defined in, for example, SEQ ID NO: 169or a functional variant thereof.

The term “operably linked” as used herein refers to a functional linkagebetween the promoter sequence and the gene of interest, such that thepromoter sequence is able to initiate transcription of the gene ofinterest.

In one embodiment, the progeny plant is stably transformed with thenucleic acid construct described herein and comprises the exogenouspolynucleotide, which is heritably maintained in the plant cell. Themethod may include steps to verify that the construct is stablyintegrated. The method may also comprise the additional step ofcollecting seeds from the selected progeny plant.

In an alternative embodiment, the method comprises introducing at leastone mutation into the plant genome to increase the activity of an AAP,as defined herein.

In one embodiment, the mutation is the insertion of at least one or moreadditional copy of an AAP with increased activity as defined herein. Forexample, the mutation may comprise the insertion of at least one or moreadditional copy of a nucleic acid encoding an AAP8 polypeptide asdefined in SEQ ID NO: 2 (Cvi allele) or 3 (Col-0 allele) or 4 or afunctional variant or homolog thereof, such that the sequence isoperably linked to a regulatory sequence.

In another embodiment, the method comprises introducing at least onemutation into at least one AAP gene. Preferably, the method comprisesintroducing at least one mutation into the, preferably endogenous,nucleic acid sequence encoding an AAP polypeptide. As used herein, theterm “endogenous” may refer to the native or natural sequence in theplant genome. In one embodiment, the endogenous amino acid sequence ofAAP8 is defined in SEQ ID NO: 1 (Ler allele) or a functional variant orhomologue thereof. More preferably, the nucleic acid sequence encodingan AAP comprises or consists of SEQ ID NO: 5 (genomic sequence of theLer allele) or a functional variant or homologue thereof.

The term “functional variant of a nucleic acid sequence” as used hereinwith reference to any of the sequences described herein refers to avariant gene or amino acid sequence or part of the gene or amino acidsequence that retains the biological function of the full non-variantsequence. A functional variant also comprises a variant of the gene ofinterest that has sequence alterations that do not affect function, forexample in non-conserved residues. Also encompassed is a variant that issubstantially identical, i.e. has only some sequence variations, forexample in non-conserved residues, compared to the wild type sequencesas shown herein and is biologically active. Alterations in a nucleicacid sequence which result in the production of a different amino acidat a given site that do not affect the functional properties of theencoded polypeptide are well known in the art. For example, a codon forthe amino acid alanine, a hydrophobic amino acid, may be substituted bya codon encoding another less hydrophobic residue, such as glycine, or amore hydrophobic residue, such as valine, leucine, or isoleucine.Similarly, changes which result in substitution of one negativelycharged residue for another, such as aspartic acid for glutamic acid, orone positively charged residue for another, such as lysine for arginine,can also be expected to produce a functionally equivalent product.Nucleotide changes which result in alteration of the N-terminal andC-terminal portions of the polypeptide molecule would also not beexpected to alter the activity of the polypeptide. Each of the proposedmodifications is well within the routine skill in the art, as isdetermination of retention of biological activity of the encodedproducts.

In one embodiment, a functional variant has at least 25%, 26%, 27%, 28%,29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%,43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%,57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%,71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%,85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, orat least 99% overall sequence identity to the non-variant nucleic acidor amino acid sequence.

The term homolog, as used herein, also designates an AAP8 geneorthologue from other plant species. A homolog may have, in increasingorder of preference, at least 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%,33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%,47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%,61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%,75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%,89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or at least 99%overall sequence identity to the amino acid represented by any of SEQ IDNO: 1 to 4 or to the nucleic acid sequences as shown by SEQ ID NOs: 5 to8. In one embodiment, overall sequence identity is at least 37%. In oneembodiment, overall sequence identity is at least 70%, 71%, 72%, 73%,74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, mostpreferably 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or at least 99%.Functional variants of an AAP8 homolog are also within the scope of theinvention.

Examples of AAP8 homologues are described in SEQ ID Nos 9 to 166.Specifically, the amino acid sequence of AAP8 homolog may be selectedfrom one of SEQ ID Nos 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31,33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67,69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101,103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129,131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157,159, 161, 163 or 165 or a functional variant thereof. In a furtherembodiment, the nucleic acid sequence of an AAP8 homolog may be selectedfrom SEQ ID Nos 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36,38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72,74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106,108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134,146, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162,164 or 166 or a functional variant thereof.

In one embodiment, where the homolog is rice, the amino acid sequence ofthe AAP8 homolog comprises or consists of SEQ ID NO: 9 or 13 or afunctional variant thereof, and the nucleic acid sequence of the AAP8homolog comprises or consists of SEQ ID NO: 10 or 14 or a functionalvariant thereof.

In a further embodiment, where the homolog is soybean, the amino acidsequence of the AAP8 homolog comprises or consists of SEQ ID NO: 31 or afunctional variant thereof, and the nucleic acid sequence of the AAP8homolog comprises or consists of SEQ ID NO: 32 or a functional variantthereof.

In a further embodiment, where the homolog is maize, the amino acidsequence of the AAP8 homolog comprises or consists of SEQ ID NO: 63 or afunctional variant thereof, and the nucleic acid sequence of the AAP8homolog comprises or consists of SEQ ID NO: 64 or a functional variantthereof.

In a further embodiment, where the homolog is B. napus, the amino acidsequence of the AAP8 homolog comprises or consists of SEQ ID NO: 123 ora functional variant thereof, and the nucleic acid sequence of the AAP8homolog comprises or consists of SEQ ID NO: 124 or a functional variantthereof.

In a further embodiment, where the homolog is B. rapa, the amino acidsequence of the AAP8 homolog comprises or consists of SEQ ID NO: 139,141 or 143 or a functional variant thereof, and the nucleic acidsequence of the AAP8 homolog comprises or consists of SEQ ID NO: 140,142 or 144 or a functional variant thereof.

In a further embodiment, where the homolog is B. oleracea, the aminoacid sequence of the AAP8 homolog comprises or consists of SEQ ID NO:157 or a functional variant thereof, and the nucleic acid sequence ofthe AAP8 homolog comprises or consists of SEQ ID NO: 158 or a functionalvariant thereof.

In a further embodiment, where the homolog is barley, the amino acidsequence of the AAP8 homolog comprises or consists of SEQ ID NO: 131 ora functional variant thereof, and the nucleic acid sequence of the AAP8homolog comprises or consists of SEQ ID NO: 132 or a functional variantthereof.

In a further embodiment, where the homolog is wheat, the amino acidsequence of the AAP8 homolog comprises or consists of SEQ ID NO: 135 or136 or a functional variant thereof, and the nucleic acid sequence ofthe AAP8 homolog comprises or consists of SEQ ID NO: 138 or 140 or afunctional variant thereof.

In a further embodiment, the AAP polypeptide of the invention comprisesthe following conserved motif. Preferably, the at least one mutation isin at least one of these residues, more preferably in the first residue(i.e. the X residue):

(SEQ ID NO: 167) XFWPLTVY

wherein X is any amino acid, but preferably is an A, S or G.

In an alternative embodiment, the AAP polypeptide comprises an aminoacid transporter motif (referred to herein as “Aa_trans motif”) asdefined below or a functional variant thereof and preferably, the atleast one mutation is in the amino acid transporter motif.

Aa_trans motif: SEQ ID NO: 168RTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWVAGTTVLVAFAIITYYTSTLLADCYRSPDSITGTRNYNYMGVVRSYLGGKKVQLCGVAQYVNLVGVTIGYTITASISLVAIGKSNCYHDKGHKAKCSVSNYPYMAAFGIVQIILSQLPNFHKLSFLSIIAAVMSFSYASIGIGLAIATVASGKIGKTELTGTVIGVDVTASEKVWKLFQAIGDIAFSYAFTTILIEIQDTLRSSPPENKVMKRASLAGVSTTTVFYILCGCIGYAAFGNQAPGDFLTDFGFYEPYWLIDFANACIALHLIGAYQVYAQPFFQFVEENCNKKWPQSNFINKEYSSKVPLLGKCRVNLFRLVWRTCYVVLTTFVAMIFPFFNAILGLLGAFVFWPLTVYFPVAMHIAQAKVKKYSRRWLALNLLVLVCLIVSALAAVGSIIGLI

Accordingly, in one embodiment, there is provided a method of increasingseed yield in a plant as described herein, the method comprisingincreasing the activity of an AAP polypeptide as described herein,wherein the AAP comprises or consists of one of the following sequences:

-   -   a. a nucleic acid sequence encoding an AAP polypeptide as        defined in SEQ ID NO: 2, 3, 4, 9, 11, 13, 15, 17, 19, 21, 23,        25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55,        57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87,        89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115,        117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141,        143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163 or 165 or        a functional variant thereof; or    -   b. a nucleic acid sequence as defined in SEQ ID NO: 6, 7, 8, 10,        12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42,        44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74,        76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104,        106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130,        132, 134, 146, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156,        158, 160, 162, 164 or 166 or a functional variant thereof; or    -   c. a nucleic acid sequence encoding an AAP polypeptide, wherein        the polypeptide comprises an amino acid transporter motif as        defined in SEQ ID NO: 168 or a variant thereof, wherein the        variant has at least 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%,        83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%,        96%, 97%, 98%, or at least 99% overall sequence identity to SEQ        ID NO: 167; or    -   d. a nucleic acid sequence encoding an AAP polypeptide, wherein        the polypeptide comprises the sequence defined in SEQ ID NO: 168        or a variant thereof, wherein the variant has at least 75%, 76%,        77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,        90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or at least 99%        overall sequence identity to SEQ ID NO: 168;    -   wherein the functional variant has at least 75%, 76%, 77%, 78%,        79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%,        92%, 93%, 94%, 95%, 96%, 97%, 98%, or at least 99% overall        sequence identity to the sequences in (a) or (b) and/or wherein        the functional variant encodes an AAP polypeptide and is capable        of binding under stringent hybridisation conditions as defined        herein to one of the sequences in (a), (b), (c) or (d).

In one embodiment, the mutation in the nucleic acid sequence encoding anAAP polypeptide may be selected from one of the following mutationtypes:

-   -   1. a “missense mutation”, which is a change in the nucleic acid        sequence (e.g. a change in one or more nucleotides) that results        in the substitution of one amino acid for another amino acid        (also known as a nonsynonymous substitution);    -   2. an “insertion mutation” of one or more nucleotides or one or        more amino acids, due to one or more codons having been added in        the coding sequence of the nucleic acid;    -   3. a “deletion mutation” of one or more nucleotides or of one or        more amino acids, due to one or more codons having been deleted        in the coding sequence of the nucleic acid;

In one embodiment the mutation is a missense mutation (nonsynonymoussubstitution).

In one embodiment, the one or more mutations in the AAP nucleic acidsequence results in an amino acid substitution at position 410 in SEQ IDNO: 1 or a homologous position in a homologous sequence. Preferably,said mutation arises from a substitution of one or more nucleotides inthe nucleic acid sequence of AAP8. In one embodiment, the mutation is atposition 2635 of SEQ ID NO: 5 or a homologous position in a homologoussequence.

In a further embodiment, the method may comprise introducing one or moreadditional mutations, preferably at position 277 and/or 374 of SEQ IDNO: 1 or a homologous position in a homologous sequence.

In a further embodiment, the nonsense mutation in the nucleic acidsequence causes a substitution of one amino acid for another in theresulting amino acid sequence. In one embodiment, the mutation is thesubstitution of one hydrophobic amino acid for another hydrophobic aminoacid. For example, the substituted residue may be selected from alanine,isoleucine, leucine, methionine, phenylalanine, tryptophan, tyrosine andvaline. More preferably the substituted residue is selected from valine,isoleucine and alanine. Most preferably the substituted residue isalanine.

“By at least one mutation” is meant that where the AAP gene is presentas more than one copy or homologue (with the same or slightly differentsequence) there is at least one mutation in at least one gene.Preferably all genes are mutated.

The skilled person would understand that suitable homologues and thehomologous positions in these sequences can be identified by sequencecomparisons and identifications of conserved domains. There arepredictors in the art that can be used to identify such sequences. Thefunction of the homologue can be identified as described herein and askilled person would thus be able to confirm the function. Homologouspositions can thus be determined by performing sequence alignments oncethe homologous sequence has been identified. For example, AAP8homologues can be identified using a BLAST search of the plant genome ofinterest using the Arabidopsis AAP8 as a query.

Identification of the homologous position in any AAP8 homologoussequence can be performed by making a multiple sequence alignment of thecandidate sequence with the Arabidopsis AAP8. In particular, theconserved amino acid transporter motif can be aligned using any knownmultiple sequence alignment program (e.g. DNAMAN) with the correspondingmotif in a candidate homologous sequence to identify the homologousposition.

Thus, the nucleotide sequences of the invention and described herein canalso be used to isolate corresponding sequences from other organisms,particularly other plants, for example crop plants. In this manner,methods such as PCR, hybridization, and the like can be used to identifysuch sequences based on their sequence homology to the sequencesdescribed herein. Topology of the sequences and the characteristicdomain structure can also be considered when identifying and isolatinghomologs. Sequences may be isolated based on their sequence identity tothe entire sequence or to fragments thereof. In hybridizationtechniques, all or part of a known nucleotide sequence is used as aprobe that selectively hybridizes to other corresponding nucleotidesequences present in a population of cloned genomic DNA fragments orcDNA fragments (i.e., genomic or cDNA libraries) from a chosen plant.The hybridization probes may be genomic DNA fragments, cDNA fragments,RNA fragments, or other oligonucleotides, and may be labelled with adetectable group, or any other detectable marker. Methods forpreparation of probes for hybridization and for construction of cDNA andgenomic libraries are generally known in the art and are disclosed inSambrook, et al., (1989) Molecular Cloning: A Library Manual (2d ed.,Cold Spring Harbor Laboratory Press, Plainview, N.Y.).

In one embodiment, the homologous position and the homologous amino acidand nucleotide sequence of AtAAP8 is selected from one of the positionsand amino acid and nucleotide sequences in the table of FIG. 12 .

In one embodiment, the mutation is introduced using mutagenesis (i.e.any site-directed mutagenesis method) or targeted genome editing. Thatis, in one embodiment, the invention relates to a method and plant thathas been generated by genetic engineering methods as described above,and does not encompass naturally occurring varieties.

Targeted genome modification or targeted genome editing is a genomeengineering technique that uses targeted DNA double-strand breaks (DSBs)to stimulate genome editing through homologous recombination(HR)-mediated recombination events. In one embodiment, the mutation isintroduced using ZFNs, TALENs or CRISPR/Cas9.

In a preferred embodiment, the targeted genome editing technique isCRISPR. The use of this technology in genome editing is well describedin the art, for example in U.S. Pat. No. 8,697,359 and references citedherein. In short, CRISPR is a microbial nuclease system involved indefence against invading phages and plasmids. CRISPR loci in microbialhosts contain a combination of CRISPR-associated (Cas) genes as well asnon-coding RNA elements capable of programming the specificity of theCRISPR-mediated nucleic acid cleavage (sgRNA). Three types (I-III) ofCRISPR systems have been identified across a wide range of bacterialhosts. One key feature of each CRISPR locus is the presence of an arrayof repetitive sequences (direct repeats) interspaced by short stretchesof non-repetitive sequences (spacers). The non-coding CRISPR array istranscribed and cleaved within direct repeats into short crRNAscontaining individual spacer sequences, which direct Cas nucleases tothe target site (protospacer). The Type II CRISPR is one of the mostwell characterized systems and carries out targeted DNA double-strandbreak in four sequential steps. First, two non-coding RNA, the pre-crRNAarray and tracrRNA, are transcribed from the CRISPR locus. Second,tracrRNA hybridizes to the repeat regions of the pre-crRNA and mediatesthe processing of pre-crRNA into mature crRNAs containing individualspacer sequences. Third, the mature crRNA:tracrRNA complex directs Cas9to the target DNA via Watson-Crick base-pairing between the spacer onthe crRNA and the protospacer on the target DNA next to the protospaceradjacent motif (PAM), an additional requirement for target recognition.Finally, Cas9 mediates cleavage of target DNA to create adouble-stranded break within the protospacer.

One major advantage of the CRISPR-Cas9 system, as compared toconventional gene targeting and other programmable endonucleases is theease of multiplexing, where multiple positions or sites on genes can bemutated simultaneously simply by using multiple sgRNAs each targeting adifferent site. In addition, where two sgRNAs are used flanking agenomic region, the intervening section can be deleted or inverted(Wiles et al., 2015). In the present invention, multiple sgRNAs can beused to simultaneously introduce two or more mutations, for example, thespecific mutations described above, into the AAP8 gene. In thisembodiment, self-cleaving RNAs or cleavable RNA molecules, such as csy4,ribozyme or tRNA sequences can be used to process a single constructinto multiple sgRNAs.

Cas9 is thus the hallmark protein of the type II CRISPR-Cas system, andis a large monomeric DNA nuclease guided to a DNA target sequenceadjacent to the PAM (protospacer adjacent motif) sequence motif by acomplex of two noncoding RNAs: CRISPR RNA (crRNA) and trans-activatingcrRNA (tracrRNA). The Cas9 protein contains two nuclease domainshomologous to RuvC and HNH nucleases. The HNH nuclease domain cleavesthe complementary DNA strand whereas the RuvC-like domain cleaves thenon-complementary strand and, as a result, a blunt cut is introduced inthe target DNA. Heterologous expression of Cas9 together with an sgRNAcan introduce site-specific double strand breaks (DSBs) into genomic DNAof live cells from various organisms. Codon optimized versions of Cas9,which is originally from the bacterium Streptococcus pyogenes, can alsobe used to increase efficiency. Cas9 orthologues may also be used, suchas Staphylococcus aureus (SaCas9) or Streptococcus thermophiles(StCas9).

The single guide RNA (sgRNA) is the second component of the CRISPR/Cassystem that forms a complex with the Cas9 nuclease. sgRNA is a syntheticRNA chimera created by fusing crRNA with tracrRNA. The sgRNA guidesequence located at its 5′ end confers DNA target specificity.Therefore, by modifying the guide sequence, it is possible to createsgRNAs with different target specificities. The canonical length of theguide sequence is 20 bp. In plants, sgRNAs have been expressed usingplant RNA polymerase III promoters, such as U6 and U3. Accordingly,using techniques known in the art it is possible to design sgRNAmolecules that targets the AAP gene as described herein. In oneembodiment, the method comprises using any of the nucleic acidconstructs or sgRNA molecules described herein.

Alternatively, Cpf1, which is another Cas protein, can be used as theendonuclease. Cpf1 differs from Cas9 in several ways: Cpf1 requires aT-rich PAM sequence (TTTV) for target recognition, Cpf1 does not requirea tracrRNA, and as such only crRNA is required unlike Cas9 and theCpf1-cleavage site is located distal and downstream to the PAM sequencein the protospacer sequence (Li et al., 2017). Furthermore, afteridentification of the PAM motif, Cpf1 introduces a sticky-end-like DNAdouble-stranded break with several nucleotides of overhang. As such, theCRISPR/CPf1 system consists of a Cpf1 enzyme and a crRNA.

Cas9 and Cpf1 expression plasmids for use in the methods of theinvention can be constructed as described in the art. Cas9 or Cpf1 andthe one or more sgRNA molecule may be delivered as separate or as asingle construct. Where separate constructs are used for the delivery ofthe CRISPR enzyme (i.e. Cas9 or Cpf1) and the sgRNA molecule(s), thepromoters used to drive expression of the CRISPR enzyme/sgRNA moleculemay be the same or different. In one embodiment, RNA polymerase (Pol)II-dependent promoters can be used to drive expression of the CRISPRenzyme. In another embodiment, Pol III-dependent promoters, such as U6or U3, can be used to drive expression of the sgRNA.

In one embodiment, the method uses a sgRNA to introduce a targeted SNPor mutation, in particular one of the substitutions described hereininto a AAP gene. As explained below, the introduction of a template DNAstrand, following a sgRNA-mediated snip in the double-stranded DNA, canbe used to produce a specific targeted mutation (i.e. a SNP) in the geneusing homology directed repair. In an alternative embodiment, at leastone mutation may be introduced into the AAP gene, particularly at thepositions described above, using any CRISPR technique known to theskilled person. In another example, sgRNA (for example, as describedherein) can be used with a modified Cas9 protein, such as nickase Cas9or nCas9 or a “dead” Cas9 (dCas9) or a Cas9 nickase (Cas9n) fused to a“Base Editor”—such as an enzyme, for example a deaminase such ascytidine deaminase, or TadA (tRNA adenosine deaminase) or ADAR orAPOBEC. These enzymes are able to substitute one base for another. As aresult no DNA is deleted, but a single substitution is made (Kim et al.,2017; Gaudelli et al. 2017).

The genome editing constructs may be introduced into a plant cell usingany suitable method known to the skilled person. In an alternativeembodiment, any of the nucleic acid constructs described herein may befirst transcribed to form a preassembled Cas9-sgRNA ribonucleoproteinand then delivered to at least one plant cell using any of the abovedescribed methods, such as lipofection, electroporation, biolisticbombardment or microinjection.

Specific protocols for using the above-described CRISPR constructs wouldbe well known to the skilled person. As one example, a suitable protocolis described in Ma & Liu (“CRISPR/Cas-based multiplex genome editing inmonocot and dicot plants”) incorporated herein by reference.

Genetically Altered or Modified Plants and Methods of Producing SuchPlants

In another aspect of the invention, there is provided a geneticallyaltered plant, part thereof or plant cell, characterised in that theplant expresses an AAP polypeptide with increased activity. In a furtherembodiment, the plant is characterised by an increase in seed yield.

In one embodiment, the plant or plant cell may comprise a nucleic acidconstruct comprising a nucleic acid encoding an AAP8 polypeptide asdefined in SEQ ID NO: 2, 3 or 4 or a functional variant or homologthereof, as defined herein. In one embodiment, the construct is stablyincorporated into the genome.

In an alternative embodiment, the plant may be produced by introducing amutation into the plant genome by any of the above-described methods. Inone embodiment, the mutation is the insertion of at least one additionalcopy of a nucleic acid encoding an AAP with increased activity asdefined herein. For example, the mutation may comprise the insertion ofat least one or more additional copy of a nucleic acid encoding an AAP8polypeptide as defined in SEQ ID NO: 2 (Cvi allele) or 3 (Col-0 allele)or 4 or a functional variant or homolog thereof, such that the sequenceis operably linked to a regulatory sequence. In an alternativeembodiment, the mutation is a substitution at position 410 of SEQ ID NO:1 or at a homologous position in a homologous sequence, as definedherein. Preferably the mutation is introduced into at least one plantcell and a plant regenerated from the at least one mutated plant cell.

The terms “introduction”, “transfection” or “transformation” as referredto herein encompass the transfer of an exogenous polynucleotide orconstruct (such as a nucleic acid construct or a genome editingconstruct as described herein) into a host cell, irrespective of themethod used for transfer. Plant tissue capable of subsequent clonalpropagation, whether by organogenesis or embryogenesis, may betransformed with a genetic construct of the present invention and awhole plant regenerated there from. The particular tissue chosen willvary depending on the clonal propagation systems available for, and bestsuited to, the particular species being transformed. Exemplary tissuetargets include leaf disks, pollen, embryos, cotyledons, hypocotyls,megagametophytes, callus tissue, existing meristematic tissue (e.g.,apical meristem, axillary buds, and root meristems), and inducedmeristem tissue (e.g., cotyledon meristem and hypocotyl meristem). Theresulting transformed plant cell may then be used to regenerate atransformed plant in a manner known to persons skilled in the art.

The transfer of foreign genes into the genome of a plant is calledtransformation. Transformation of plants is now a routine technique inmany species. Any of several transformation methods known to the skilledperson may be used to introduce one or more genome editing constructs ofinterest into a suitable ancestor cell. The methods described for thetransformation and regeneration of plants from plant tissues or plantcells may be utilized for transient or for stable transformation.

Transformation methods include the use of liposomes, electroporation,chemicals that increase free DNA uptake, injection of the DNA directlyinto the plant (microinjection), gene guns (or biolistic particledelivery systems (bioloistics)) as described in the examples,lipofection, transformation using viruses or pollen and microprojection.Methods may be selected from the calcium/polyethylene glycol method forprotoplasts, ultrasound-mediated gene transfection, optical or lasertransfection, transfection using silicon carbide fibers, electroporationof protoplasts, microinjection into plant material, DNA or RNA-coatedparticle bombardment, infection with (non-integrative) viruses and thelike. Transgenic plants can also be produced via Agrobacteriumtumefaciens mediated transformation, including but not limited to usingthe floral dip/Agrobacterium vacuum infiltration method as described inClough & Bent (1998) and incorporated herein by reference.

Optionally, to select transformed plants, the plant material obtained inthe transformation is, as a rule, subjected to selective conditions sothat transformed plants can be distinguished from untransformed plants.For example, the seeds obtained in the above-described manner can beplanted and, after an initial growing period, subjected to a suitableselection by spraying. A further possibility is growing the seeds, ifappropriate after sterilization, on agar plates using a suitableselection agent so that only the transformed seeds can grow into plants.As described in the examples, a suitable marker can bebar-phosphinothricin or PPT. Alternatively, the transformed plants arescreened for the presence of a selectable marker, such as, but notlimited to, GFP, GUS (β-glucuronidase). Other examples would be readilyknown to the skilled person. Alternatively, no selection is performed,and the seeds obtained in the above-described manner are planted andgrown and AAP activity levels measured at an appropriate time usingstandard techniques in the art. This alternative, which avoids theintroduction of transgenes, is preferable to produce transgene-freeplants.

Following DNA transfer and regeneration, putatively transformed plantsmay also be evaluated, for instance using PCR to detect the presence ofthe gene of interest, copy number and/or genomic organisation.Alternatively or additionally, integration and expression levels of thenewly introduced DNA may be monitored using Southern, Northern and/orWestern analysis, both techniques being well known to persons havingordinary skill in the art.

The method may further comprise selecting one or more mutated plants,preferably for further propagation. The selected plants may bepropagated by a variety of means, such as by clonal propagation orclassical breeding techniques. For example, a first generation (or T1)transformed plant may be selfed and homozygous second-generation (or T2)transformants selected, and the T2 plants may then further be propagatedthrough classical breeding techniques. The generated transformedorganisms may take a variety of forms. For example, they may be chimerasof transformed cells and non-transformed cells; clonal transformants(e.g., all cells transformed to contain the expression cassette); graftsof transformed and untransformed tissues (e.g., in plants, a transformedrootstock grafted to an untransformed scion).

In a further related aspect of the invention, there is also provided amethod of obtaining a genetically modified plant as described herein,the method comprising

-   -   a. selecting a part of the plant;    -   b. transfecting at least one cell of the part of the plant of        paragraph (a) with at least one nucleic acid construct as        described herein or at least one sgRNA molecule as described        herein, using the transfection or transformation techniques        described above;    -   c. regenerating at least one plant derived from the transfected        cell or cells;    -   d. selecting one or more plants obtained according to        paragraph (c) that show increased activity of an AAP        polypeptide.

In a further embodiment, the method also comprises the step of screeningthe genetically modified plant for the introduction of one or moreadditional copies of an AAP nucleic acid, as described herein, or forthe introduction of one or more substitutions into the endogenous AAPgenomic sequence. In one embodiment, the method comprises obtaining aDNA sample from a transformed plant and carrying out DNA amplificationto detect one of the mutations described above. In a further embodiment,the methods comprise generating stable T2 plants preferably homozygousfor the mutation.

A genetically altered plant of the present invention may also beobtained by transference of any of the sequences of the invention bycrossing, e.g., using pollen of the genetically altered plant describedherein to pollinate a wild-type or control plant, or pollinating thegynoecia of plants described herein with other pollen that does notcontain at least one of the above-described mutations. The methods forobtaining the plant of the invention are not exclusively limited tothose described in this paragraph; for example, genetic transformationof germ cells from the ear of wheat could be carried out as mentioned,but without having to regenerate a plant afterward.

In a further aspect of the invention there is provided a plant obtainedor obtainable by the above-described methods. Also included in the scopeof the invention is the progeny obtained from the plants.

The plant according to the various aspects of the invention may be amonocot or a dicot plant. A dicot plant may be selected from thefamilies including, but not limited to Asteraceae, Brassicaceae (egBrassica napus, Thlaspi arvense), Chenopodiaceae, Cucurbitaceae,Leguminosae (Caesalpiniaceae, Aesalpiniaceae Mimosaceae, Papilionaceaeor Fabaceae), Malvaceae, Rosaceae or Solanaceae. For example, the plantmay be selected from lettuce, sunflower, Arabidopsis, broccoli, spinach,water melon, squash, cabbage, tomato, potato, yam, capsicum, tobacco,cotton, okra, apple, rose, strawberry, alfalfa, bean, soybean, field(fava) bean, pea, lentil, peanut, chickpea, apricots, pears, peach,grape vine or citrus species.

A monocot plant may, for example, be selected from the familiesArecaceae, Amaryllidaceae or Poaceae. For example, the plant may be acereal crop, such as wheat, rice, barley, maize, oat, sorghum, rye,millet, buckwheat, turf grass, Italian rye grass, sugarcane or Festucaspecies, or a crop such as onion, leek, yam or banana.

Preferably, the plant is a crop plant. By crop plant is meant any plantwhich is grown on a commercial scale for human or animal consumption oruse. Preferred plants are maize, wheat, rice, oilseed rape, cannabis,sorghum, soybean, pennycress, potato, tomato, grape, barley, pea, bean,field bean, lettuce, cotton, sugar cane, sugar beet, broccoli or othervegetable brassicas or poplar.

The term “plant” as used herein encompasses whole plants, ancestors andprogeny of the plants and plant parts, including seeds, fruit, shoots,stems, leaves, roots (including tubers), flowers, tissues and organs,wherein each of the aforementioned comprise the nucleic acid constructas described herein. The term “plant” also encompasses plant cells,suspension cultures, callus tissue, embryos, meristematic regions,gametophytes, sporophytes, pollen and microspores, again wherein each ofthe aforementioned comprises the nucleic acid construct as describedherein.

The invention also extends to harvestable parts of a plant of theinvention as described herein, but not limited to seeds, leaves, fruits,flowers, stems, roots, rhizomes, tubers and bulbs. The aspects of theinvention also extend to products derived, preferably directly derived,from a harvestable part of such a plant, such as dry pellets or powders,oil, fat and fatty acids, starch or proteins. Another product that mayderived from the harvestable parts of the plant of the invention isbiodiesel. The invention also relates to food products and foodsupplements comprising the plant of the invention or parts thereof. Inone embodiment, the food products may be animal feed. In another aspectof the invention, there is provided a product derived from a plant asdescribed herein or from a part thereof.

In a further aspect of the invention there is provided a method forproducing a food or feed product with increased protein content, saidmethod comprising

-   -   a. producing a plant wherein the activity of an AAP polypeptide,        preferably AAP8 or homologue as described herein, is increased;    -   b. obtaining a seed from said plant;    -   c. producing a food or feed product from said seed.

In a preferred embodiment, the plant part or harvestable product is aseed. Therefore, in a further aspect of the invention, there is provideda seed produced from a genetically altered plant as described herein. Inan alternative embodiment, the plant part is pollen, a propagule orprogeny of the genetically altered plant described herein. Accordingly,in a further aspect of the invention there is provided pollen, apropagule or progeny of the genetically altered plant as describedherein.

A control plant as used herein according to all of the aspects of theinvention is a plant which has not been modified according to themethods of the invention. Accordingly, in one embodiment, the controlplant does not have increased activity of an AAP polypeptide. In analternative embodiment, the plant been genetically modified, asdescribed above. In one embodiment, the control plant is a wild typeplant. The control plant is typically of the same plant species,preferably having the same genetic background as the modified plant.

In another aspect of the invention, there is provided a nucleic acidconstruct comprising a nucleic acid sequence encoding a AAP8 polypeptideas defined in SEQ ID NO: 2 (the Cvi allele) or 3 (the Col-0 allele) or 4or a functional variant or homolog thereof (as defined herein). In afurther embodiment, the nucleic acid construct comprises a nucleic acidsequence comprising or consisting of a nucleic acid sequence as definedin SEQ ID NO: 6 or 7, or 8 or functional variant or homolog thereof.Preferably, the nucleic acid is operably linked to a regulatory sequenceas defined herein.

In a further aspect of the invention, there is provided an isolatedcell, preferably a plant cell or an Agrobacterium tumefaciens cell,expressing a nucleic acid construct as described herein. Furthermore,the invention also relates to a culture medium or kit comprising anisolated plant cell or an Agrobacterium tumefaciens cell expressing thenucleic acid construct described herein.

There is also provided the use of the nucleic acid construct describedherein to increase seed yield.

Method of Screening Plants for Naturally Occurring High Levels of AAPActivity

In another aspect of the invention, there is provided a method forscreening a population of plants and identifying and/or selecting aplant that has increased activity of at least one AAP polypeptide,wherein the method comprises detecting in the plant germplasm at leastone polymorphism correlated with increased activity of an AAPpolypeptide, as described herein. Preferably, said plant has anincreased seed yield.

In one embodiment, the polymorphism is a substitution. In one specificembodiment, said polymorphism may comprise at least one substitution atposition 2635 of SEQ ID NO: 5, 6, 7 or 8 or a homologous position in ahomologous sequence, as described herein.

In a further embodiment, the method may further comprise detecting oneor more additional polymorphisms, wherein preferably the one or moreadditional polymorphisms are selected from:

-   -   a substitution at position 2044 of SEQ ID NO: 5, 6, 7 or 8 or a        homologous position in a homologous sequence; and/or    -   a substitution at position 2526 of SEQ ID NO: 5, 6, 7 or 8 or a        homologous position in a homologous sequence.

Examples of homologous positions in a number of homologous sequences areshown in FIG. 12 . Accordingly, in one embodiment, the at least onepolymorphism is selected from one of the genomic mutations shown in FIG.12 .

Suitable tests for assessing the presence of a polymorphism would bewell known to the skilled person, and include but are not limited to,Isozyme Electrophoresis, Restriction Fragment Length Polymorphisms(RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily PrimedPolymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting(DAF), Sequence Characterized Amplified Regions (SCARs), AmplifiedFragment Length polymorphisms (AFLPs), Simple Sequence Repeats(SSRs-which are also referred to as Microsatellites), and SingleNucleotide Polymorphisms (SNPs). In one embodiment, Kompetitive AlleleSpecific PCR (KASP) genotyping is used.

In one embodiment, the method comprises

-   a) obtaining a nucleic acid sample from a plant and-   b) carrying out nucleic acid amplification of one or more AAP,    preferably AAP8 alleles using one or more primer pairs.

In a further embodiment, the method may further comprise introgressingthe chromosomal region comprising an AAP polymorphism into a secondplant or plant germplasm to produce an introgressed plant or plantgermplasm. Preferably, said second plant will display an increase inseed yield compared to a control or wild-type plant that does not carrythe polymorphism.

In a further aspect of the invention there is provided a method forincreasing seed yield, the method comprising

-   -   a. screening a population of plants for at least one plant with        at least one AAP polymorphism as described herein;    -   b. further modulating the activity of an AAP protein, as        described herein, in said plant by introducing and expressing a        nucleic acid construct comprising a nucleic acid encoding an AAP        polypeptide as described herein, or introducing at least one        mutation into the nucleic acid sequence encoding an AAP as        described herein.

While the foregoing disclosure provides a general description of thesubject matter encompassed within the scope of the present invention,including methods, as well as the best mode thereof, of making and usingthis invention, the following examples are provided to further enablethose skilled in the art to practice this invention and to provide acomplete written description thereof. However, those skilled in the artwill appreciate that the specifics of these examples should not be readas limiting on the invention, the scope of which should be apprehendedfrom the claims and equivalents thereof appended to this disclosure.Various further aspects and embodiments of the present invention will beapparent to those skilled in the art in view of the present disclosure.

“and/or” where used herein is to be taken as specific disclosure of eachof the two specified features or components with or without the other.For example “A and/or B” is to be taken as specific disclosure of eachof (i) A, (ii) B and (iii) A and B, just as if each is set outindividually herein.

Unless context dictates otherwise, the descriptions and definitions ofthe features set out above are not limited to any particular aspect orembodiment of the invention and apply equally to all aspects andembodiments which are described.

The foregoing application, and all documents and sequence accessionnumbers cited therein or during their prosecution (“appln citeddocuments”) and all documents cited or referenced in the appln citeddocuments, and all documents cited or referenced herein (“herein citeddocuments”), and all documents cited or referenced in herein citeddocuments, together with any manufacturer's instructions, descriptions,product specifications, and product sheets for any products mentionedherein or in any document incorporated by reference herein, are herebyincorporated herein by reference, and may be employed in the practice ofthe invention. More specifically, all referenced documents areincorporated by reference to the same extent as if each individualdocument was specifically and individually indicated to be incorporatedby reference.

The invention is now described in the following non-limiting example.

Example

To understand natural allelic variation at seed size loci, we sought toidentify the QTL genes for seed size in Arabidopsis. Cvi (Cape VerdeIslands) and Ler (Landsburg erecta) are two Arabidopsis accessions. Cviseeds were obviously larger and heavier than Ler seeds (FIG. 7 )(Alonso-Blanco et al., 1999). By using one recombinant inbred linepopulation from Ler an Cvi, a QTL locus for seed size was previouslymapped into the top region of Chromosome I (Alonso-Blanco et al., 1999).To identify the gene corresponding to this QTL for seed size, weobtained the chromosome segment substitution lines (CSSL) thatintrogressed genomic regions from Cvi accession to the Ler geneticbackground, which covered this QTL region (Keurentjes et al., 2007). Theline CSSL-LCN1-3-3 showed larger and heavier seeds than Ler (FIG. 7 ),suggesting that this line contained a genomic region from Cvi, whichcontributes to large and heavy seed phenotypes. To confirm this, webackcrossed the line CSSL-LCN1-3-3 with Ler and generated an F₂population. Using this F₂ population, we mapped a major QTL locus forgrain size and weight on Chromosome I (SSW1) (FIGS. 3A and 3B). Wefurther backcrossed the line CSSL-LCN1-3-3 with Ler for five times andgenerated a near-isogenic line NIL-SSW1^(Cvi) in the Ler background.

We next investigated grain size and weight of Ler and NIL-SSW1^(Cvi). Asshown in FIG. 1 , NIL-SSW1^(Cvi) seeds were significantly larger andheavier than Ler seeds. Consistent with this, the NIL-SSW1^(Cvi) embryoswere slightly big compared with Ler embryos (FIG. 1B). The changes inseed size often influence the size of seedlings. Supporting this, the10-d-old NIL-SSW1^(Cvi) cotyledons were bigger than Ler cotyledons(FIGS. 1C and 1D). By contrast, plant morphology of NIL-SSW1^(Cvi) wassimilar to that of Ler. The sizes of NIL-SSW1^(Cvi) leaves and floralorgans were comparable with that of Ler. These results indicate thatSSW1 regulates seed size and weight in Arabidopsis.

The maternal and/or zygotic tissues have been known to determine thesize of a seed (Li and Li, 2016), we therefore asked whether SSW1 actsmaternally or zygotically. The reciprocal cross experiments between Lerand NIL-SSW1^(Cvi) were conducted. The size of seeds from NIL-SSW1^(Cvi)plants pollinated with Ler pollen or NIL-SSW1^(Cvi) pollen wassignificantly larger than that from the self-pollinated Ler plants (FIG.2A). By contrast, Ler plants pollinated with NIL-SSW1^(Cvi) pollenproduced similar-sized seeds to Ler plants pollinated with their ownpollen. These results of four crosses show that SSW1 maternally affectsseed growth. We further examined the size of Ler/Ler F₂,Ler/NIL-SSW1^(Cvi) F₂, NIL-SSW1^(Cvi)/Ler F₂ andNIL-SSW1^(Cvi)/NIL-SSW1^(Cvi) F₂ seeds. Ler/NIL-SSW1^(Cvi) F₂,NIL-SSW1^(Cvi)/Ler F₂ and NIL-SSW1^(Cvi)/NIL-SSW1^(Cvi) F₂ seeds weresignificantly larger than Ler/Ler F₂ seeds (FIG. 2B). Thus, thesefindings reveal that SSW1 controls seed size through maternal tissues.These data also indicate that the SSW1^(Cvi) allele is a dominantallele, while the SSW1^(Ler) allele is a recessive allele.

The integuments surrounding the ovule have been proposed to affect thefinal size of a seed after fertilization (Adamski et al., 2009; Du etal., 2014; Garcia et al., 2005; Schruff et al., 2006; Xia et al., 2013).Considering that SSW1 affects seed size through maternal tissues, weexamined whether SSW1 could control seed size through the maternalinteguments. We firstly observed mature ovules before fertilization. Asshown in FIGS. 2C and 2D, the NIL-SSW1^(Cvi) mature ovules wereobviously larger than Ler ovules. NIL-SSW1^(Cvi) ovules had longer outerintegument than Ler ovules (FIG. 2G).

Considering that the growth of the integument is influenced by celldivision and cell expansion, we investigated cell number and cell sizeof the outer integuments in Ler and NIL-SSW1^(Cvi) ovules. The outerintegument NIL-SSW1^(Cvi) ovules contained more cells than that of Lerovules (FIG. 2H). By contrast, outer integument cells in NIL-SSW1^(Cvi)ovules showed similar length to those in Ler ovules (FIG. 2I). Thesedata indicated that SSW1 influences cell proliferation in theinteguments of ovules. We further investigated the effect of SSW1 oncell proliferation and cell expansion in the integuments of developingseeds. At 6 days after pollination (6 DAP), the outer integument cellsin Ler and NIL-SSW1^(Cvi) seeds absolutely stop division (FIG. 2H). Theouter integument in NIL-SSW1^(Cvi) seeds contained more cells than thatin Ler seeds (FIG. 2H). By contrast, the length of the outer integumentcells in NIL-SSW1^(Cvi) seeds was comparable with that in Ler seeds(FIG. 2I). Taken together, these data demonstrate that SSW1 affects cellproliferation in the maternal integuments of ovules and developingseeds.

To identify the QTL gene for seed size and weight (SSW1), we generatedlarge F₂ population from a cross between the original line CSSL-LCN1-3-3and Ler. This QTL locus was mapped into the short arm of the chromosome1 between markers Cvi-m5 and Cvi-m18. We genotyped 10048 F₂ plants usingmarkers Cvi-m5 and Cvi-m18 and identified 867 recombinants. To identifythe gene underlying the SSW1 locus, we developed another four markers(Cvi-m40, Cvi-m39, Cvi-m51 and Cvi-m33) in mapping region. We thenselected 33 plants with recombinations between these six markers toperform progeny test. Based on progeny test results, we narrowed thecandidate gene region containing the SSW1 locus to 21.71 kb betweenmarkers Cvi-m51 and Cvi-m33, which contains four genes (At1g10010,At1g10020, At1g10030 and At1g10040) (FIGS. 3A and 3B). Considering thatnatural mutations could happen in the promoter region, we firstlyexamined expression levels of these four genes in Ler andNIL-SSW1^(Cvi). As shown in FIG. 3C, expression levels of these fourgenes in NIL-SSW1^(Cvi) were comparable with those in Ler, suggestingthat natural allelic variation in SSW1 might not affect its expressionlevel. We then sequenced these four genes in Ler, Cvi and NIL-SSW1^(Cvi)Sequence comparison revealed that the predicted amino acid sequencesencoded by At1g10020, At1g10030 and At1g10040 in NIL-SSW1^(Cvi) areexactly the same as those in Ler, suggesting that it is unlikely thatAt1g10020, At1g10030 and At1g10040 are responsible for seed sizevariation. By contrast, the region of the At1g10010 gene inNIL-SSW1^(Cvi) and Cvi contains 12 single nucleotide polymorphismscompared with that in Ler, including 8 single nucleotide polymorphismsin introns and 4 single nucleotide polymorphisms in exons (FIG. 3D andFIG. 11 ). Four single nucleotide polymorphisms in exons contain onenucleotide change (C2204A) in the exon 5 that is a synonymous mutation,one nucleotide substitution (C2044T) in the exon 5 that led to an aminoacid change from Ala to Val, one nucleotide substitution (G2526A) in theexon 6 that caused an amino acid change from Val to Ile, and onenucleotide substitution (T2635C) in the exon 6 that caused an amino acidchange from Val to Ala (FIGS. 3D and 3F). We further developed themarker SSW1-m according to the mutation C1961T in the At1g10010 gene,which was co-segregated with the seed size phenotype (FIG. 3A).Therefore, these results suggest that At1g10010 is a candidate gene forSSW1.

To testify whether natural variation in the At1g10010 gene causes largeseeds in Cvi, we conducted a genomic complementation test. Ourreciprocal crosses revealed that the Cvi allele is a dominant allele andthe Ler allele is a recessive allele (FIGS. 2A and 2B). We thereforeintroduced a genomic fragment from Cvi that includes 2,631-bp flankingsequence of 5′ UTR, the At1g10010 gene and 671-bp flanking sequence of3′UTR (gSSW1^(Cvi)-COM) into Ler. Transgenic plants (gSSW1^(Cvi)-COM)produced large and heavy seeds, like those observed in NIL-SSW1^(Cvi)(FIG. 3G and FIG. 8 ), indicating that At1g10010 is the SSW1 gene. Wealso introduced the SSW1 genomic fragment from Ler (gSSW1^(Ler)-COM)into Ler. As shown in FIG. 8 , the size of gSSW1^(Ler)-COM seeds wassimilar to that of Ler, indicating that there was no dosage effect intransgenic plants (FIG. 8 ). These results further support thatAt1g10010 is the SSW1 gene.

As three nucleotide polymorphisms resulted in amino acid changes betweenLer and Cvi (FIG. 3F), we analyzed the sequences of the SSW1 gene inArabidopsis accessions from 1001 genome project (Genomes Consortium.Electronic address and Genomes, 2016). According to these threepolymorphisms, these Arabidopsis accessions contained three types ofnatural allelic variation in the SSW1 gene, including SSW1^(Cvi),SSW1^(Ler) and SSW1^(Col-0) types. Most Arabidopsis accessions (93.16%)are the SSW1^(Col-0) type, 4.37% Arabidopsis accessions possess theSSW1^(Ler) type, and 2.47% Arabidopsis accessions belong to theSSW1^(Cvi) type (FIG. 3E). Arabidopsis accessions with the SSW1^(Col-0)type grow in different regions of the world. Interestingly, we foundthat Arabidopsis accessions with the SSW1^(Ler) type are predominantlydistributed in Sweden and Germany, while accessions with the SSW1^(Cvi)type mainly grow in the south of Russia and Spain.

SSW1 encodes the amino acid permease 8 (AAP8) containing an amino acidtransporter motif (FIG. 3F). Homologs of AAP8 were found in Arabidopsisand crops. In Arabidopsis, AAP8 belongs to the AAP family that consistsof eight members (AAP1-AAP8) (Okumoto, 2002). The AAP family membershave been proposed to participate in a variety of physiologicalprocesses in plants, such as amino acid transport and xylem-phloemtransfer (Tegeder, 2012). Arabidopsis AAP8 mediates amino acid uptakeinto seeds, but its role in seed size control has not been characterizedin detail.

To determine expression of SSW1/AAP8, we conducted quantitativereal-time RT-PCR analysis. Relatively higher expression of AAP8 wasfound in roots, inflorescences, and developing siliques, consistent witha previous study (Okumoto, 2002). AAP8 has been shown to localize in theplasma membrane when SSW1/AAP8-GFP fusion protein was transientlyexpressed in N. benthamian leaves (Santiago and Tegeder, 2016). However,the subcellular localization of AAP8 in Arabidopsis plants has not beendescribed. We generated 35S:GFP-AAP8 transgenic plants to investigatethe subcellular localization of AAP8 in Arabidopsis. GFP signal in35S:GFP-AAP8 transgenic plants was found at the cell periphery. Toexamine whether AAP8-GFP was localized in cell walls or the plasmamembrane, we used a high concentration of sucrose to induce plasmolysis.GFP signal was detected in the plasma membrane. Thus, these results showthat SSW1/AAP8 is a plasma membrane protein in Arabidopsis.

To further investigate the function of loss-of-function of SSW1/AAP8 inseed size, we obtained two mutants (aap8-1/SALK_092908 andapp8-101/SALK_122286C) harboring T-DNA insertions in the first intron ofthe At1g10010 gene, respectively (FIG. 3D). We crossed app8-1 andapp8-101 to Col-0 for three times before we investigated theirphenotypes. Expression of SSW1/AAP8 was hardly detected in app8-1 andapp8-101 mutants (FIG. 3H), suggesting that they are null alleles. Wemeasured seed area and seed weight of app8-1 and app8-101. As shown inFIG. 3I, seed area and seed weight of app8-1 and app8-101 weresignificantly decreased in comparison to those of Col-0. We introducedthe genomic fragment (gSSW1^(Cvi)-COM) from Cvi accession into app8-1mutant. The gSSW1^(Cvi)-COM fragment complemented the seed sizephenotype of the app8-1 mutant, indicating that loss of function ofSSW1/AAP8 results in small and light seeds (FIG. 3J).

We then performed the reciprocal cross experiments between Col-0 andapp8-1 by hand pollination. As shown in FIG. 9A, app8-1 plantspollinated with Col-0 pollen or app8-1 pollen produced smaller F₁ seedscompared with the F₁ seeds of the self-pollinated Col-0 plants.Col-0/Col-0 F₂, Col-0/app8-1 F₂, and app8-1/Col-0 F₂ seeds weresignificantly larger than app8-1/app8-1 F₂ seeds (FIG. 9B). Thus, theseresults further demonstrate that SSW1 is required in maternal tissues tocontrol seed size. We then examined cell number and cell size in theouter integuments and found that SSW1 influences cell proliferation inthe maternal integuments of ovules and developing seeds (FIGS. 9D and9E).

As natural allelic variation in SSW1 contributes to seed size and weightdifferences between Cvi and Ler, we asked whether natural allelicvariation in SSW1 influences the amino acid permease activity ofSSW1/AAP8. The yeast mutant strain 22Δ8AA can not use γ-aminobutyricacid, arginine, proline, aspartate, glutamate or citrulline as solenitrogen sources (Okumoto, 2002). AAP8 has been reported to complementthe mutant strain 22Δ8AA (Okumoto, 2002). We therefore expressed theSSW1/AAP8 gene from Cvi (pFL61-SSW1^(Cvi)) and Ler (pFL61-SSW1^(Ler)) inthe mutant strain 22Δ8AA, respectively. The 22Δ8AA cells withpFL61-SSW1^(Cvi) formed colonies on plates containing 1 mM and 2 mM ASPas sole nitrogen source after 4 days. By contrast, the 22Δ8AA cells withpFL61-SSW1^(Ler) formed colonies on plates containing 3 mM ASP as solenitrogen source after 4 days. However, the growth vigor of the 22Δ8AAcells with pFL61-SSW1^(Ler) was obviously lower than that of the 22Δ8AAcells with pFL61-SSW1^(Cvi) on plates supplying 1 mM, 2 mM or 3 mM ASPas sole nitrogen source. These results indicate that the SSW1 from Cvi(SSW1^(Cvi)) has higher amino acid permease activity than that from theLer allele (SSW1^(Ler)).

To quantify the activity differences between SSW1^(Cvi) and SSW1^(Ler),we cultured the mutant stain 22Δ8AA harboring pFL61, pFL61-SSW1^(Ler)and pFL61-SSW1^(Cvi) constructs in liquid medium with 1 mM ASP as solenitrogen source and monitored their growth dynamics by measuring theoptical density (OD) at 600 nm every 12 hours. As shown in FIG. 4B, theOD_(600 nm) of the mutant stain 22Δ8AA transformed with pFL61-SSW1^(Cvi)increased drastically after 96 hours, and plateaued after 156 hours. Bycontrast, the mutant stain 22Δ8AA transformed with pFL61-SSW1^(Ler)showed a slightly faster growth than control (pFL61) (FIG. 4B). Thesedata indicate that SSW1^(Cvi) has higher activity in transporting ASPthan SSW1^(Ler), and SSW1^(Ler) still possesses weak activity intransporting ASP.

As SSW1^(Col-0) has an amino acid change (I374V) compared withSSW1^(Cvi) we investigated the activity of SSW1^(Col-0) in transportingamino acid in yeast cells (FIG. 4A). The mutant stain 22Δ8AA harboringpFL61-SSW1^(Col-0) construct was cultured in liquid medium with 1 mM ASPas sole nitrogen source, and the growth dynamic was detected bymeasuring the optical density (OD) at 600 nm every 12 hours. The growthdynamic of the mutant stain 22Δ8AA transformed with pFL61-SSW1^(Col-0)was similar to that of the mutant stain 22Δ8AA transformed withpFL61-SSW^(Cvi) (FIG. 4B), indicating that SSW1^(Col-0) has similaramino acid transport activity to SSW1^(Cvi) and possesses higher aminoacid transport activity than SSW1^(Ler). This result also suggests thatonly one amino acid change (I374 V) does not significantly affect thetransport activity.

As there are three amino acid differences betweenSSW1^(Ler(A277;V374;V410)) and SSW1^(Cvi (V277;I374;A410)), we askedwhich amino acid plays a predominant role in determining the activity ofSSW1. To test this, we generated AL/SSW1^(Ler(A277;V374;V410)),AC/SSW1^(Cvi (V277;I374;A410)), AM1/SSW1^((V277;V374;V410)),AM2/SSW1^((A277;I374;V410)), AM3/SSW1^((A277;V374;A410)),AN1/SSW1^((A277;V374;A410)), and AN2/SSW1^(Col-0(V277;V374;A410))constructs and transformed into the yeast mutant strain 22Δ8AA (FIG.4A). As shown in FIG. 4B, AN2/SSW1^(Col-0), AM3/SSW1^((A277;V374;A410))and AN1/SSW1^((A277,I374;A410)) showed similar transport efficiency toSSW1^(Cvi) while the activity of AM2/SSW1^((A277;I374;V410)) andAM1/SSW1^((V277;V374;V410)) were comparable with that of SSW1^(Ler).Thus, these results indicate that the change in the amino acid V410A ismainly responsible for the activity differences between SSW1^(Cvi) andSSW1^(Ler).

As SSW1 encodes an amino acid permease that has been proposed totransport amino acids to developing seeds (Schmidt et al., 2007), weanalyzed the content of free amino acids in young siliques and matureseeds of NIL-SSW1^(Cvi) and Ler by Gas Chromatography-Mass Spectrometer(GC-MS). In young siliques, the contents of some free amino acids suchas alanine, serine, aspartic acid, asparagine, and glutamic acid weresignificantly increased in NIL-SSW1^(Cvi), while the contents of someamino acids remain the same as Ler (FIG. 5A). In mature seeds, thecontents of several amino acids (e.g. valine, alanine, serine, glycine,glutamic acid and tryptophan) in NIL-SSW1^(Cvi) were significantlyincreased compared with that in Ler (FIG. 5B). Total amino acid contentsin NIL-SSW1^(Cvi) siliques and seeds were increased compared with thosein Ler siliques and seeds (FIG. 5C). These results indicate that theSSW1^(Cvi) natural allele increases amino acid contents.

We also assayed the content of free amino acids in young siliques andmature seeds of Col-0 and aap8-1. In young siliques, the contents ofsome free amino acids such as proline, glycine, aspartic acid, glutamicacid, asparagine and glutamine were significantly decreased in aap8-1,while the contents of some amino acids were similar to those in Col-0.In mature seeds, the contents of several amino acids (e.g. valine,leucine, isoleucine, serine, glycine, threonine, aspartic acid, glutamicacid, phenylalanine and tryptophan) in aap8-1 were significantlydecreased compared with that in Col-0. In addition, total amino acidcontents in the siliques and seeds of app8-1 were lower than those inwild-type (Col-0) siliques and seeds.

We then analyzed the content of soluble proteins in Ler andNIL-SSW1^(Cvi) dry seeds by SDS-PAGE. The contents of 12S globulin αsubunit, 12S globulin β subunit, 2S albumin large subunit and 2S albuminsmall subunit in NIL-SSW1^(Cvi) seeds were obviously increased comparedwith those in Ler seeds (FIG. 5D). These results indicate that theSSW1^(Cvi) natural allele seeds contain more storage proteins than Ler.We then measured the content of soluble proteins in dry seeds of Ler andthree gSSW1^(Cvi)-Com transgenic lines. The contents of 12S globulin αsubunit, 12S globulin β subunit, 2S albumin large subunit and 2S albuminsmall subunit in seeds of gSSW1^(Cvi)-Com transgenic lines wereobviously increased compared with those in Ler seeds (FIG. 10 ).

As AAP8/SSW1 exhibits the highest similarity to Arabidopsis AAP1, whichhas been reported influencing seed weight (Sanders, 2009), we askedwhether there are any genetic relationship between aap8-1 and aap1 inseed size control. To test this, we obtained aap1-101 (Salk_078312)(FIGS. 6A to 6C). The aap1-101 seeds were significantly smaller thanCol-0 seeds (FIGS. 6D and 6E), consistent with the result that aap1seeds were lighter than wild-type seeds (Sanders, 2009). We crossedaap8-1 with app1-101 and generated aap8-1 app1-101 double mutant. Theseed size and weight of the aap8-1 aap1-101 double mutants were notsignificantly decreased compared with those of aap8-1 (FIGS. 6D and 6E),suggesting that AAP8 may act, at least in part, genetically with AAP1 toaffect seed size and weight.

Discussion

Seed size is an important yield trait and is controlled by quantitativetrait loci. Several QTLs for seed size have been mapped in Arabidopsis,but the genes corresponding to these QTLs have not been cloned yet. Inthis study, we cloned the first QTL gene for seed size and weight (SSW1)in Arabidopsis and find that natural allelic variation in SSW1contributes to seed size, weight and quality. SSW1 encodes an amino acidpermease (AAP8) that transports amino acids into seeds. Natural allelicvariation in SSW1 affects the amino acid permease activity, therebyinfluencing the contents of free amino acids and storage proteins inseeds. Therefore, these results reveal the genetic and molecular basisfor natural variation in seed size, weight and quality control,suggesting that it is an important target for improving both seed sizeand quality in crops.

Several QTL loci for seed size were mapped in different chromosomes ofArabidopsis using the recombinant inbred line population from Ler andCvi (Alonso-Blanco et al., 1999), but the QTL genes for seed size havenot been identified in Arabidopsis. In this study, we fine-mapped amajor QTL locus for grain size and weight (SSW1) and cloned the SSW1gene in Arabidopsis. NIL-SSW1^(Cvi) produced larger and heavier grainsthan Ler. By contrast, NIL-SSW1^(Cvi) exhibited similar plantarchitecture, flower size and leaf size to Ler, suggesting that SSW1mainly controls seed size and weight in Arabidopsis. Cellularobservations show that SSW1 controls seed size by promoting cellproliferation during ovule and seed development. SSW1 encodes the aminoacid permease AAP8. In Arabidopsis, AAP8 belongs to the AAP family thatconsists of eight members (AAP1-AAP8) (Okumoto, 2002). The AAP familymembers have been proposed to participate in a variety of physiologicalprocesses in plants, such as amino acid transport and xylem-phloemtransfer (Tegeder, 2012). OsAAP6 has been proved to enhance grainprotein content and nutritional quality greatly in rice (Peng et al.,2014). In Arabidopsis, AAP8 mediates amino acid uptake into developingseeds, but its role in seed size control has not been characterized indetail. Here we demonstrate natural allelic variations in AAP8contribute to grain size and weight. AAP8 acts as a positive factor ofseed size and weight control in Arabidopsis. Interestingly, a previouslystudy proposed that loss of function of AAP8 resulted in significantseed abortion (Schmidt et al., 2007) and heavy seeds (Santiago andTegeder, 2016). It is possible that seed abortion might cause heavyseeds.

In this study, we found that the NIL-SSW^(Cvi) had a similar ratio ofseed abortion to Ler. Similarly, aap8-1 and aap8-101 mutations did notaffect seed abortion compared with the wild type Col-0 under our growthconditions. We also have sufficient evidence to demonstrate thatSSW1/AAP8 positively influences seed size and weight. Expression ofSSW1/AAP8 complemented the small seed phenotype of aap8-1 (FIG. 3J). Inaddition, transformation of the genomic sequence of SSW1^(Cvi) into Lerbackground resulted in large and heavy seeds (FIG. 3G and FIG. 8 ). Thenatural allele SSW1^(Cvi) enhanced the large seed phenotype ofda1-1^(Ler) and bb-1, which have been known to form large seeds (Li etal., 2008b; Xia et al., 2013), suggesting that SSW1/AAP8 may actindependently of DA1 and BB to control seed size and also indicatingthat the SSW1^(Cvi) allele promotes seed growth in Arabidopsis. Thus,our data demonstrate that SSW1/AAP8 positively influences seed size inArabidopsis.

Sequence analyses reveal that Arabidopsis accessions possess three maintypes of natural allelic variation in the SSW1/AAP8 gene, includingSSW1^(Cvi), SSW1^(Ler) and SSW1^(Col-0) types. Most Arabidopsisaccessions contain the SSW1^(Col-0) type, 4.37% Arabidopsis accessionsare the SSW1^(Ler) type, and 2.47% Arabidopsis accessions belong to theSSW1^(Cvi) type (FIG. 3E). We found that that SSW1^(Cvi) has higheramino acid permease activity than SSW1^(Ler). SSW1^(Cvi) showed similaramino acid permease activity to SSW1^(Col-0) but higher activity thanSSW1^(Ler), indicating that the natural allele SSW1^(Ler) is a partialloss of function allele. As SSW1^(Col-0) has an amino acid change(I374V) compared with SSW1^(Cvi), I374V change may not strongly affectthe activity of SSW1. There are three amino acid differences betweenSSW1^(Ler(A277; V374;V410)) and SSW1^(Cvi (V277,I374;A410)) (FIG. 3F).Our results showed that the change in the amino acid V410A arepredominantly responsible for the differences of amino acid permeaseactivity between SSW1^(Cvi) and SSW1^(Ler). Thus, our findings revealthat natural variation in SSW1 leads to changes in amino acid permeaseactivity, there by influencing seed size and weight (FIG. 6F). Higheramino acid permease activity in Cvi accession causes large seeds (FIG.6F). Interestingly, Arabidopsis accessions with the SSW1^(Col-0) typegrow in different parts of the world, accessions with the SSW1^(Ler)type are predominantly distributes in Sweden and Germany, and accessionswith the SSW1^(Cvi) type mainly grow in the south of Russia and Spain.It is possible that the locations of SSW1^(Cvi) and SSW1^(Ler) types mayreflect the demographic history of Arabidopsis thaliana (GenomesConsortium. Electronic address and Genomes, 2016).

The growth of seeds depends on nitrogen and carbon import from thematernal tissues into developing seeds. Amino acids, the importanttransport form of nitrogen, are mainly assimilated within plant roots orleaves and then transported to developing fruits and seeds. ArabidopsisAAP8 has been reported to transport amino acids from roots to developingseeds (Schmidt et al., 2007). AAP8 was also crucial for the uptake ofamino acids into endosperm (Schmidt et al., 2007). AAP8 is expressed inmaternal tissues, such as roots, leaves, flower buds, siliques, funiculiand young seeds (Okumoto, 2002). Thus, it is possible that the deliveryof amino acids and carbon from maternal tissues (e.g. roots, leaves,flower buds and siliques) to developing seeds is important for seed sizeand weight control. Consistent with this, reciprocal cross experimentsindicate that SSW1 influences seed size through maternal tissues.Similarly, expression of sucrose transporter (AtSUC2) driven by thephloem protein 2 promoter resulted in large grains in rice (Wang et al.,2015). Arabidopsis AAP1, the closest homolog of AAP8, has been reportedto regulate import of amino acids into roots and subsequenttranslocation into the shoots as well as import of amino acids from theendosperm to the embryo (Lee et al., 2007; Sanders, 2009). Our geneticanalyses suggest that AAP8 acts, at least in part, genetically with AAP1to affect seed size and weight. It is possible that AAP8 and AAP1 mightact different steps to transport amino acids to seeds (FIG. 6F). Wefurther showed that the NIL-SSW1^(Cvi) seeds contained more free aminoacids and storage proteins than Ler seeds, indicating that AAP8regulates both seed weight and seed quality (FIGS. 5A to 5D). Thus, ourfindings reveal the genetic and molecular basis for natural variation ofSSW1/AAP8 in seed size, weight and quality control. Our currentunderstanding of natural allelic variation in SSW1/AAP8 suggests thatAAP8 and its orthologs in crops (e.g. oilseed rape and soybean) could beused to increase both seed size and seed quality in crops.

Materials and Methods

Plant Materials and Growth Conditions

The near isogenic line CSSL-LCN1-3-3 derived from a cross between twoArabidopsis thaliana ecotypes Ler (Landsberg erecta) and Cvi (Cape VerdeIslands). The CSSL-LCN1-3-3 line was backcrossed with Ler for five timesto generate the near isogenic line NIL-SSW^(Cvi). The aap8-1(SALK_092908), aap8-101 (SALK_122286C) and aap1-101 (SALK 078312) wereobtained from the NASC and backcrossed into Col-0 for three times.Arabidopsis plants were grown in greenhouse under long-day conditions at22° C.

Map-Based Cloning, Constructs and Plant Transformation

The SSW1 gene was mapped using the F₂ population of a cross betweenCSSL-LCN1-3-3 and Ler. By using this F₂ population, we mapped a majorQTL locus for grain size and weight (SSW1). This QTL locus was mappedinto the short arm of the chromosome 1 between markers Cvi-m5 andCvi-m18. To identify the gene underlying the SSW1 locus, we genotyped10048 F₂ plants with newly-developed markers in the mapping region. Weselected 33 recombinants between these markers to perform progeny test.Based on progeny test results, we narrowed the candidate gene regioncontaining the SSW1 locus to about 21.71 kb between markers Cvi-m51 andCvi-m33, which contains four genes (At1g10010, At1g10020, At1g10030 andAt1g10040).

The 2,631-bp flanking sequence of 5′ UTR, the At1g10010 gene and 671-bpflanking sequence of 3′UTR from SSW1^(Cvi) and SSW1^(Ler) were amplifiedusing the primers SSW1-gP-1F and SSW1-g3U-1R. To generategSSW1^(Cvi)-COM and gSSW1^(Ler)-COM constructs, we ligased PCR productto pCR8/GW/TOPO vector, and then ligased to the pMDC99 binary vectorusing LR reaction (Invitrogen). We transformed the plasmidsgSSW1^(Cvi)-COM and gSSW1^(Ler)-COM into the Ler using Agrobacteriumtumefaciens line GV3101, and then selected transformants using MS mediumsupplied with hygromycin (30 μg/mL). We transformed the plasmidgSSW1^(Cvi)-COM into the aap8-1 using the same way.

The 1425-bp coding region of SSW1/AAP8 gene from Col-0 was amplifiedusing primers SSW1-cS-F and SSW1-cE-R. To constructp35S:GFP-SSW1^(Col-0), we subcloned PCR product to pCR8/GW/TOPO vector,and then ligased to the pMDC43 binary vector using LR reaction(Invitrogen). We transformed the plasmid p35S:GFP-SSW1^(Col-0) into theCol-0 using Agrobacterium tumefaciens line GV3101, and selectedtransformants using MS medium supplied with hygromycin (30 μg/mL).

Morphological and Cellular Analysis

Mature dry seeds from 3rd-10th siliques of main stems, cotyledons,leaves and floral organs were harvested to measure their sizes asdescribed previously (Zhang et al., 2015). Mature ovules and developingseeds were photographed using differential interference contrast (DIC)microscope (Leica DM2500) to count cells in the outer integument andmeasure the length of the outer integument by Image J software.

Subcellular Localization

The Zeiss LSM 710 NLO confocal microscope was used to observe GFPfluorescence signals. Petals were treated with 25 μg/μL propidium iodideand 1 μg/mL fm4-64 to stain cell wall and plasma membrane, and treatedwith 30% sucrose solution for plasmolysis.

RNA Isolation, RT-PCR and Quantitative Real-Time RT-PCR Analysis

RNAprep pure plant kit (Tiangen) was used to extract total RNA.SuperScript III reverse transcriptase (Invitrogen) was used to reverselytranscribe into cDNA. The 7500 Real-Time PCR System (Applied Biosystems)was used to conduct Quantitative real-time RT-PCR (QRT-PCR). An internalcontrol is ACTIN2 mRNA.

Protein and Free Amino Acid Analysis

Extraction of soluble protein was conducted according to Sanders et. al.(Sanders, 2009) with modification. A batch of 100 dry mature seeds weregrounded in 200 μL extraction buffer [10% (v/v) glycerol, 100 mMTris-HCl, 2% (v/v) β-mercaptoethanol and pH 8.0, 0.5% (w/v) SDS]. Theresulting 40 μL supernatant after centrifugation in 20,000 g for 10 minwas moved to a 1.5 mL microfuge tube and again centrifugated in 20,000 gfor 5 min. 4 μL loading buffer [10% (v/v) glycerol, 62.5 mM Tris-HCl,β-mercaptoethanol, 8 M Urea and, 2% (w/v) SDS]. 20 μL supernatant wasadded into 2 μL bromophenol blue, boiled at 98° C. for 15 min and loadedonto a 15% SDS-PAGE for about 130 min at 100 V after a briefcentrifugation.

Free amino acid assays were conducted according to a previously report(Tan et al., 2011). The concentration of free amino acids was calculatedby internal standard method, and normalized to the unit dry weight ofsample.

Yeast Growth Assay

The coding region sequence of SSW1/AAP8 gene was amplified fromSSW1^(Cvi) and Ler cDNA library using primers L-cS-pFL61-infu-F1 andL-cE-pFL61-infu-R2, and then subcloned into yeast expression vectorpFL61 to generate the AL and AC plasmids, respectively. The AL and ACconstructs and the empty vector were transformed into 22Δ8AA. Thetransformants were selected on SD/-Ura with Agar media (Clontech Cat.No. 630315, Lot. No. 1504553A). Growth assays were performed on M.ammedia (Jacobs et al., 1980) lacing uracil with 2.5% (w/v) agar andaspartate at concentrations of 1, 2, 3 mM. Monoclonal transformants wereincubated in liquid YPDA media and cultured at 30° C., 200 rpm for about8-12 h until OD_(600 nm)≈1. After centrifugation precipitates werewashed with 0.9% NaCl for three times. We equalized OD_(600 nm) of allsamples of yeast cells to about 0.5 with sterilized 0.9% NaCl, and thenstroke 10 μL mixture onto plates and culture at 30° C. All experimentswere repeated three times with independent colonies.

Site-directed mutagenesis PCR products harboring different nucleotidevariations were amplified using primers L-cS-pFL61-infu-F1,L-cE-pFL61-infu-R2 and L-M1-R1, L-M1-F2, L-M2-R1, L-M2-F2, L-M3-R1,L-M3-F1, L-N1-R1, L-N1-F2, L-N2-R1, L-N2-F2, by leading false priminginto primers, and then PCR products were subcloned in pFL61 to generateplasmids AM1, AM2, AM3, AN1 and AN2. Plasmids AL, AC, AM1, AM2, AM3,AN1, AN2 and empty vector were transformed into yeast strain 22Δ8AA.

For yeast growth dynamics assays, monoclonal transformants wereincubated in liquid YPDA media and cultured at 30° C., 200 rpm for about8-12 h until OD_(600 nm)≈1. Precipitates after centrifugation werewashed with 0.9% NaCl for three times. Yeast cells were added into 5 mLM.am media with 1 mM aspartate (the OD_(600 nm)≈0.1), cultured at 30°C., and used to measure the OD_(600 nm) every 12 hours.

Sequence Listing

Examples of suitable mutation positions (in the wild-type sequence) ormutated nucleotides/amino acids (in the mutated sequences) arehighlighted. The invention is not limited to these mutation positions.

SEQ ID NO: 1: AtAAP8^(Ler )(protein)

SEQ ID NO: 2 AtAAP8^(Cvi )(protein)

SEQ ID NO: 3: AtAAP8^(Col-0 )(protein)

SEQ ID NO: 4: AtAAP8 A410 (protein)

SEQ ID NO: 5: AtAAP8^(Ler )(genomic) (Introns are underlined)AGGGAGTACTCTAATAAGACGACCTCTGTCAATAACTCTCTTCCCCTCTCTTCTCTCCTCTGGTTCAGTGGTTCTCTCACAATGATGGACGCATACCACAATCCTTCGGCGGTGGAGTCGGGTGACGCCGCCGTGAAAAGCGTCGACGACGATGGTCGAGAGAAGAGAACGGGAACATTTTGGACGGCGAGTGCGCACATAATCACGGCGGTCATAGGCTCAGGGGTGCTGTCGTTGGCTTGGGCTATAGCACAGCTTGGTTGGGTGGCAGGAACCACAGTTTTGGTCGCTTTCGCCATCATTACTTACTACACGTCCACCTTGCTCGCCGACTGTTACCGTTCGCCGGACTCCATCACCGGAACACGCAACTATAATTACATGGGCGTCGTCCGATCTTACCTTGGTATGGATTCATATAAACAAATTCATTTTGTGTCTTTATCAGCATTGTTTTTCACAGATTTTTCAGTTTTCTAGACATTTTTTCTCAGATGAACAAGGATTTTGTTCATTTGATATCATTTAGATTTTGCCTAACTAGTCTCAATTTACGACATGTGTTTTGATTTTCTTCCATTTCTGTCACAATGATGATGGCTGGCGAAAGAAAAAAAATCTGATCTAAAAATATATATTTAATGCTAAGTTGGAATTTGTAAATCTACAGTATAATTGGCTCATTTCAACAATTTCTTTCCATGTAAATTTGTTGAAGAACATTATTGTTGTTGAACAATGAAAGAAAAAAATATGGTTGTTAGAAAAAAATGATTTACGATTTTGCCAAGTGTGCATGCTCTTTCATGGGAAGATATGAATTAATTATCAAAATCTATATAAAAAAAAGGAAGATAATCTTCATTCTTTTCATAACTTAGTTAATAAATTAAATTGATTAGGATTGGTAACATAGTCAATTCAATTTATCCCGTTAAAGAATGTTATAAATTCGATTGTTGACCCCTCGTTGAAAATTTGGAATTATGCGGGATGTTTAGAAACTTTGCCATAAGACCAAAAGATTGGTAGTATTTGATAGTAGTACAAGAGTAATCATTTTTCTTCTTTAATAACATAAAACGCAGGTGGTAAAAAGGTTCAGCTATGTGGAGTGGCACAGTACGTGAATCTCGTAGGGGTCACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTGTAAGTCAAAGATTCTGATTTATTTCGATTATTTTGTTATGGTTATACTAACATGTTGTTCTGAATAAAAATTACTAATAATTGTTTGATTGGTGTTTTTGTACGTCTTCGTTAGAGCGATTGGGAAATCAAATTGTTATCATGACAAGGGACATAAAGCGAAATGTTCTGTATCGAATTATCCATACATGGCGGCATTTGGGATCGTCCAGATCATTCTGAGCCAGCTTCCTAACTTCCACAAGCTCTCTTTCCTATCCATCATCGCCGCGGTTATGTCCTTCTCTTATGCGTCTATCGGAATAGGCCTAGCCATCGCTACTGTAGCAAGTACATTCCCCTTCTTTATCTTAAAACATAGTGGTTTATATGGATGATTCTTCAAAGTTGACACTAACCGTGAAAATGGTATACAATATATATGAAAGGTGGGAAGATTGGTAAGACAGAATTGACAGGGACAGTGATAGGTGTGGACGTAACTGCGTCTGAAAAAGTTTGGAAATTGTTTCAAGCGATTGGAGACATTGCCTTTTCATACGCTTTTACCACTATTCTCATCGAGATTCAGGCATGTACTACTGATTCCTACTATCTTCCGTTTACTATTGTTTTCATTTGCTTGTTATTATTAATTTCGCCAAAAAGAGGTAAAATAAGAATACCTTGAAGATAAGATGTTATTATTGATTAGAAAGGTAGGAAAAAATATAGATGGATGGATGATGGATCAAATAGTTTCATATTTTAGATATGTGAAGCTCTAAAGATAGTGACGCTCTAGTAGTATGTCTTGTTTATTTTGCAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAACGAGCAAGTCTTGCCGAGTCTCAACCACAACTGTTTTCTACATCTTGTGTGGTTGCATCGGATATGCTGCGTTCGGCAACCAAGCCCCTGGTGACTTCCTTACCGATTTTGGTTTTTACGAACCTTATTGGCTCATCGACTTTGCCAATGCTTGCATTGCTCTCCATCTAATCGGTGCCTATCAGGTATAACTCACAAACAAAAGAATAGGATAAGTGTGTAACATACATTTACCGTGTTCAAGTTCATTAAAAGTCTCATTATTGTGTTAGAATTTTTAGCTTTAACAATTCAGAAGATTGTAGAAATGGAGTTATTACTAAATATTGTTTCTAAAAAATGCTCTTTTTTTTTTTTTATCCCTGTATTATTCGCAGGTGTATGCGCAGCCGTTTTTCCAGTTTGTTGAGGAAAACTGCAACAAAAAATGGCCTCAAAGCAATTTCATCAACAAAGAATACTCGTCAAAGGTTCCTTTGCTTGGAAAATGTCGTGTCAACCTCTTCAGACTGGTTTGGAGGACATGCTATGTTGTTTTGACAACATTTGTAGCAATGATATTCCCCTTCTTCAATGCGATCTTGGGTTTGCTAGGGGCATTCGTGTTCTGGCCACTCACAGTTTATTTTCCGGTGGCAATGCACATTGCGCAGGCTAAAGTCAAGAAGTATTCTCGTAGATGGTTGGCCTTGAACCTCCTCGTATTGGTTTGCTTGATCGTCTCGGCCCTTGCCGCCGTAGGATCCATCATTGGCTTAATTAATAGTGTCAAGTCATACAAGCCCTTCAAGAATTTAGACTAGTGTGACTTATAATCTATGTTTGCCAAAAAAAAACCTTGTGATCCATATGAAATTTATTTCATGCTAAATATTTAGTACTTAATGTTTCTCCAAATAATGTGACGTTCTGTTTTCAGCTATGTTAAAAAACAAAATGCTAACTTGTGTATTAGTACTAAAATTTATGAAAATGTATTAGTTATTGATTTATTTTTAGGACTACAATTATTGAATCAACATTGGATGTTTGAGTCCCATGAGATATGGATTTCAGCTTTTTTCAAATTCGTGTGGTTGTGTCAATTTCGAGTTATTATTATTTATTTTGCTTAATGGATTGTCGGGGAAATCTTGAAAACAGACACTCACAGATTGTGTAATTTATTTGGTTTGGTGTGTCCTACATAAGTTGCTATCACATCTTATGTATTGGAGGAGTTGGGCAATAGAGGATCAAGGCAAGTTTGGTTTTCTATTAACGTTTCTACTCTGCATTTGCTTACAAAGTCATTTTCAAGGTTTTGTGGTCGTATGTCACTTGATGGSEQ ID NO: 6: AtAAP8^(Cvi) (genomic) (Introns are underlined)AGGGAGTACTCTAATAAGACGACCTCTGTCAATAACTCTCTTCCCCTCTCTTCTCTCCTCTGGTTCAGTGGTTCTCTCACAATGATGGACGCATACCCAATCCTTCGGCGGTGGAGTCGGGTGACGCCGCCGTGAAAAGCGTCGACGACGATGGTCGAGAGAAGAGAACGGGAACATTTTGGACGGCGAGTGCGCACATAATCACGGCGGTCATAGGCTCAGGGGTGCTGTCGTTGGCTTGGGCTATAGCACAGCTTGGTTGGGTGGCAGGAACCACAGTTTTGGTCGCTTTCGCCATCATTACTTACTACACGTCCACCTTGCTCGCCGACTGTTACCGTTCGCCGGACTCCATCACCGGAACACGCAACTATAATTACATGGGCGTCGTCCGATCTTACCTTGGTATGGATTCATATAAACAAATTCATTTTGTGTCTTTATCAGCATTGTTTTTCACAGATTTTTCAGTTTTCTAGACATTTTTTCTCAGATGAACAAGGATTTTGTTCATTTGATATCATTTAGATTTTGCCTAACTAGTCTCAATTTAGGACATGTGTTTTGATTTTCTTCCATTTCTGTCACAATGATGATGGCTGGCGAAAGAAAAAAAATCTGATCTAAAAATATATATTTAATGCTAAGTTGGAATTTGTAAATCTACAGTATAATTGGCTCATTTCAACAATTTTTTACCATGTAAATTTGTTGAAGAACATTATTGTTGTTGAACAATGAAAGAAAAAAATATGGTTGTTAGAAAAAAATGATTTACGATTTTGCCAAGTGTGCATGCTCTTTCATGGGAAGATATGAATTAATTATCAAAATCTATATAAAAAAAAGGAAGATAATCTTCATTCTTTCATAACTTAGTTAATAAATTAAATTGATTAGGATTGGTAACATAGTCAATTCAATTTATCCCGTTAAAGAATGTTATAAATTCGATTGTTGACCCCTCGTTGAAAATTTGGAATTATGCGGGATGTTTAGAAACTTTGCCATAAGACCAAAAGATTGGTAGTATTTGATAGTAGTACAAGAGTAATCATTTTTCTTCTTTAATAACATAAAACGCAGGTGGTAAAAAGGTTCAGCTATGTGGAGTGGCACAGTACGTGAATCTCGTAGGGGTCACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTGTAAGTCAAAGATTCTGATTTATTTCGATTATTTTGTTATGGTTATACTAACATGTTGTTCTGAATAAAATTACTAATAATTGTTTGATTGGTGTTTTTGTACGTCTTCGTTAGAGCGATTGGGAAATCAAATTGTTATCATGACAAGGGACATAAAGCGAAATGTTCTGTATCGAATTATCCATACATGGCGGCATTTGGGATCGTCCAGATCATTCTGAGCCAGCTTCCTAACTTCCACAAGCTCTCTTTCCTATCCATCATCGCCGCGGTTATGTCCTTCTCTTATGCGTCTATCGGAATAGGCCTAGCCATCGCTACTGTAGCAAGTACATTCCCCTTCTTTATCTTAAAACATAGTGGTTTATATGGATGATTCTTCAAAGTTGACACTAACCGTGAAAATGGTATACAATATATATGAAAGGTGGGAAGATTGGTAAGACAGAATTGACAGGGACAGTGATAGGTGTGGACGTAACTGCGTCTGAAAAAGTTTGGAAATTGTTTCAAGCGATTGGAGACATTGCCTTTTCATACGCTTTTACCACTATTCTCATCGAGATTCAGGCATGTACTACTGATTCCTACTATCTTCCGTTTACTATTGTTTTCATTTGCTTGTTATTATTAATTTCGCCAAAGAGAGGTAAATAAGAATACCTTGAAGATAAGATGTTATTATTAATTAGACAGTTAGGAAAAAATATAGATGGATGGATGATGGATAAAAATAGTTTCATATTTTAGATATGTGAAGCTCTAAAGATAGTGACGCTCTAGTAGTATGTCTTGTTTATTTTGCAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAACGAGCAAGTCTTGTCGGAGTCTCAACCACAACTGTTTTCTACATCTTGTGTGGTTGCATCGGATATGCTGCGTTCGGCAACCAAGCCCCTGGTGACTTCCTTACCGATTTTGGTTTTTACGAACCTTATTGGCTCATCGACTTTGCCAATGCTTGCATTGCTCTCCATCTAATAGGTGCCTATCAGGTATAACTCACAAACAAAAGAATAGGATAAGTGTGTAACATACATTTACCGTGTTCAAGTTCATTAAAAGTCTCATTATTGTGTTAGAATTTTTAGCTTTAACAATTCAGAAGATTGTAGAAATGGAGTTATTACTAAATATTGTTTCTAAAAAATGCTCTTTTTTTTTTTTTATCCCTGTATTATTCGCAGGTGTATGCGCAGCCGTTTTTCCAGTTTGTTGAGGAAAACTGCAACAAAAAATGGCCTCAAAGCAATTTCATCAACAAAGAATACTCGTCAAAGGTTCCTTTGCTTGGAAAATGTCGTATCAACCTCTTCAGACTGGTTTGGAGGACATGCTATGTTGTTTTGACAACATTTGTAGCAATGATATTCCCCTTCTTCAATGCGATCTTGGGTTTGCTAGGGGCACTCGCGTTCTGGCCACTCACAGTTTATTTTCCGGTGGCAATGCACATTGCGCAGGCTAAAGTCAAGAAGTATTCTCGTAGATGGTTGGCCTTGAACCTCCTCGTATTGGTTTGCTTGATCGTCTCGGCCCTTGCCGCCGTAGGATCCATCATTGGCTTAATTAATAGTGTCAAGTCATACAAGCCCTTCAAGAATTTAGACTAGTGTGACTTATAATCTATGTTTGCCAAAAAAAAACCTTGTGATCCATATGAATTATATGAAATTTATTTGATGCTAAATATTTAGTACTTAATGGTTTCTCCAAATAATGTGACGTTCTGTTTTCAGCTATGTTAAAAACCAAAATGCTAACTTATGTATTAGTACTAAAATTTATGAAAATGTATTAGTTATTGATTTATTTTTAGGACTACAATTATTGAATCAACATTGGATGTTTGAGTCCCATGAGATATGGATTTCAGCTTTTTTCAAATTCGTGTGGTTGTGTCAATTTCGAGTTATTATTATTTATTTTGCTTAATGGAATTGTCGGGGAAATCTTGAAAACAGACACTCACAGATTGTGTAATTTATTTGGTTTGGTGTGTCCTACATAAGTTGCTATCACATCTTATGTATTGGAGGAGTTGGGCAATAGAGGATCAAGGCAAGTTTGGTTTTCTATTAACGTTTCTACTCTGCATTTGCTTACAAAGTCATTTTCAAGGTTTTGTGGTCGTATGTCACTTGATGGSEQ ID NO: 7: AtAAP8^(Col-0) (genomic) (Introns are underlined)AGGGAGTACTCTAATAAGACGACCTCTGTCAATAACTCTCTTCCCCTCTCTTCTCTCCTCTGGTTCAGTGGTTCTCTCACAATGATGGACGCATACAACAATCCCTCGGCGGTGGAGTCGGGTGACGCCGCCGTGAAAAGCGTCGACGACGATGGTCGAGAGAAGAGAACGGGAACATTTTGGACGGCGAGTGCGCACATAATCACGGCGGTCATAGGCTCAGGGGTGCTGTCGTTGGCTTGGGCTATAGCACAGCTTGGTTGGGTGGCAGGAACCACAGTTTTGGTCGCTTTCGCCATCATTACTTACTACACGTCCACCTTGCTCGCCGACTGTTACCGTTCGCCGGACTCCATCACCGGAACACGCAACTATAATTACATGGGCGTCGTCCGATCTTACCTTGGTATGGATTCATATAAACAAATTCATTTTGTGTCTTTATCAGCATTGTTTTTCACAGATTTTTCAGTTTTCTAGACATTTTTTCTCAGATGAACAAGGATTTTGTTCATTTGATATCATTTAGATTTTGCCTAACTAGTCTCAATTTAGGACATGTGTTTTGATTTTCTTCCATTTCTGTCACAATGATGATGGCTGGCGAAAGAAAAAAAATCTGATCTAAAAATATATATTTAATGCTAAGTTGGAATTTGTAAATCTACAGTATAATTGGCTCATTTCAACAATTTTTTACCATGTAAATTTGTTGAAGAACATTATTGTTGTTGAACAATGAAAGAAAAAAATATGGTTGTTAGAAAAAAATGATTTACGATTTTGCCAAGTGTGCATGCTCTTTCATGGGAAGATATGAATTAATTATCAAAATCTATATAAAAAAAAGGAAGATAATCTTCATTCTTTCATAACTTAGTTAATAAATTAAATTGATTAGGATTGGTAACATAGTCAATTCAATTTATCCCGTTAAAGAATGTTATAAATTCGATTGTTGACCCCTCGTTGAAAATTTGGAATTATGCGGGATGTTTAGAAACTTTGCCATAAGACCAAAAGATTGGTAGTATTTGATAGTAGTACAAGAGTAATCATTTTTCTTCTTTAATAACATAAAACGCAGGTGGTAAAAAGGTTCAGCTATGTGGAGTGGCACAGTACGTGAATCTCGTAGGGGTCACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTGTAAGTCAAAGATTCTGATTTATTTCGATTATTTTGTTATGGTTATACTAACATGTTGTTCTGAATAAAATTACTAATAATTGTTTGATTGGTGTTTTTGTACGTCTTCGTTAGAGCGATTGGGAAATCAAATTGTTATCATGACAAGGGAACATAAAGCGAAATGTTCTGTATCGAATTATCCATACATGGCGGCATTTGGGATCGTCCAGATCATTCTGAGCCAGCTTCCTAACTTCCACAAGCTCTCTTTCCTATCCATCATCGCCGCGGTTATGTCCTTCTCTTATGCGTCTATCGGAATAGGCCTAGCCATCGCTACTGTAGCAAGTACATTCCCCTTCTTTATCTTAAAACATAGTGGTTTATATGGATGATTCTTCAAAGTTGACACTAACCGTGAAAATGGTATACAATATATATGAAAGGTGGGAAGATTGGTAAGACAGAATTGACAGGGACAGTGATAGGTGTGGACGTAACTGCGTCTGAAAAAGTTTGGAAATTGTTTCAAGCGATTGGAGACATTGCCTTTTCATACGCTTTTACCACTATTCTCATCGAGATTCAGGCATGTACTACTGATTCCTACTATCTTCCGTTTACTATTGTTTTCATTTGCTTGTTATTATTAATTTCGCCAAAGAGAGGTAAAATAAGAATACCTTGAAGATAAGA

ATAGTTTCATATTTTAGATATGTGAAGCTCTAAAGATAGTGACGCTCTAGTAGTATGTCTTGTTTATTTTGCAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAACGAGCAAGTCTTGTCGGAGTCTCAACCACAACTGTTTTCTACATCTTGTGTGGTTGCATCGGATATGCTGCGTTCGGCAACCAAGCCCCTGGTGACTTCCTTACCGATTTTGGTTTTTACGAACCTTATTGGCTCATCGACTTTGCCAATGCTTGCATTGCTCTCCATCTAATAGGTGCCTATCAGGTATAACTCACAAACAAAAGAATAGGATAAGTGTGTAACATACATTTACCGTGTTCAAGTTCATTAAAAGTCTCATTATTGTGTTAGAATTTTTAGCTTTAACAATTCAGAAGATTGTAGAAATGGAGTTATTACTAAATATTGTTTCTAAAAAATGCTCTTTTTTTTTTTTTATCCCTGTATTATTCGCAGGTGTATGCGCAGCCGTTTTTCCAGTTTGTTGAGGAAAACTGCAACAAAAAATGGCCTCAAAGCAATTTCATCAACAAAGAATACTCGTCAAAGGTTCCTTTGCTTGGAAAATGTCGTGTCAACCTCTTCAGACTGGTTTGGAGGACATGCTATGTTGTTTTGACAACATTTGTAGCAATGATATTCCCCTTCTTCAATGCGATCTTGGGTTTGCTAGGGGCATTCGCGTTCTGGCCACTCACAGTTTATTTTCCGGTGGCAATGCACATTGCGCAGGCTAAAGTCAAGAAGTATTCTCGTAGATGGTTGGCCTTGAACCTCCTCGTATTGGTTTGCTTGATCGTCTCGGCCCTTGCCGCCGTAGGATCCATCATTGGCTTAATTAATAGTGTCAAGTCATACAAGCCCTTCAAGAATTTAGACTAGTGTGACTTATAATCTATGTTTGCCAAAAAAAAACCTTGTGATCCATATGAATTATATGAAATTTATTTCATGCTAAATATTTAGTACTTAATGTTTCTCCAAATAATGTGACGTTCTGTTTTCAGCTATGTTAAAAACCAAAATGCTAACTTATGTATTAGTACTAAAATTTATGAAAATGTATTAGTTATTGATTTATTTTTAGGACTACAATTATTGAATCAACATTGGATGTTTGAGTCCCATGAGATATGGATTTCAGCTTTTTTCAAATTCGTGTGGTTGTGTCAATTTCGAGTTATTATTATTTATTTTGCTTAATGGAATTGTCGGGGAAATCTTGAAAACAGACACTCACAGATTGTGTAATTTATTTGGTTTGGTGTGTCCTACATAAGTTGCTATCACATCTTATGTATTGGAGGAGTTGGGCAATAGAGGATCAAGGCAAGTTTGGTTTTCTATTAACGTTTCTACTCTGCATTTGCTTACAAAGTCATTTTCAAGGTTTTGTGGTCGTATGTCACTTGATGGSEQ ID NO: 8: AtAAP8 A410 (genomic)AGGGAGTACTCTAATAAGACGACCTCTGTCAATAACTCTCTTCCCCTCTCTTCTCTCCTCTGGTTCAGTGGTTCTCTCACAATGATGGACGCATACCACAATCCTTCGGCGGTGGAGTCGGGTGACGCCGCCGTGAAAAGCGTCGACGACGATGGTCGAGAGAAGAGAACGGGAACATTTTGGACGGCGAGTGCGCACATAATCACGGCGGTCATAGGCTCAGGGGTGCTGTCGTTGGCTTGGGCTATAGCACAGCTTGGTTGGGTGGCAGGAACCACAGTTTTGGTCGCTTTCGCCATCATTACTTACTACACGTCCACCTTGCTCGCCGACTGTTACCGTTCGCCGGACTCCATCACCGGAACACGCAACTATAATTACATGGGCGTCGTCCGATCTTACCTTGGTATGGATTCATATAAACAAATTCATTTTGTGTCTTTATCAGCATTGTTTTTCACAGATTTTTCAGTTTTCTAGACATTTTTTCTCAGATGAACAAGGATTTTGTTCATTTGATATCATTTAGATTTTGCCTAACTAGTCTCAATTTACGACATGTGTTTTGATTTTCTTCCATTTCTGTCACAATGATGATGGCTGGCGAAAGAAAAAAAATCTGATCTAAAAATATATATTTAATGCTAAGTTGGAATTTGTAAATCTACAGTATAATTGGCTCATTTCAACAATTTCTTTCCATGTAAATTTGTTGAAGAACATTATTGTTGTTGAACAATGAAAGAAAAAAATATGGTTGTTAGAAAAAAATGATTTACGATTTTGCCAAGTGTGCATGCTCTTTCATGGGAAGATATGAATTAATTATCAAAATCTATATAAAAAAAAGGAAGATAATCTTCATTCTTTCATAACTTAGTTAATAAATTAAATTGATTAGGATTGGTAACATAGTCAATTCAATTTATCCCGTTAAAGAATGTTATAAATTCGATTGTTGACCCCTCGTTGAAAATTTGGAATTATGCGGGATGTTTAGAAACTTTGCCATAAGACCAAAAGATTGGTAGTATTTGATAGTAGTACAAGAGTAATCATTTTTCTTCTTTAATAACATAAAACGCAGGTGGTAAAAAGGTTCAGCTATGTGGAGTGGCACAGTACGTGAATCTCGTAGGGGTCACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTGTAAGTCAAAGATTCTGATTTATTTCGATTATTTTGTTATGGTTATACTAACATGTTGTTCTGAATAAAATTACTAATAATTGTTTGATTGGTGTTTTTGTACGTCTTCGTTAGAGCGATTGGGAAATCAAATTGTTATCATGACAAGGGACATAAAGCGAAATGTTCTGTATCGAATTATCCATACATGGCGGCATTTGGGATCGTCCAGATCATTCTGAGCCAGCTTCCTAACTTCCACAAGCTCTCTTTCCTATCCATCATCGCCGCGGTTATGTCCTTCTCTTATGCGTCTATCGGAATAGGCCTAGCCATCGCTACTGTAGCAAGTACATTCCCCTTCTTTATCTTAAAACATAGTGGTTTATATGGATGATTCTTCAAAGTTGACACTAACCGTGAAAATGGTATACAATATATATGAAAGGTGGGAAGATTGGTAAGACAGAATTGACAGGGACAGTGATAGGTGTGGACGTAACTGCGTCTGAAAAAGTTTGGAAATTGTTTCAAGCGATTGGAGACATTGCCTTTTCATACGCTTTTACCACTATTCTCATCGAGATTCAGGCATGTACTACTGATTCCTACTATCTTCCGTTTACTATTGTTTTCATTTGCTTGTTATTATTAATTTCGCCAAAAAGAGGTAAAATAAGAATACCTTGAAGATAAGATGTTATTATTGATTAGAAAGGTAGGAAAAAATATAGATGGATGGATGATGGATCAAATAGTTTCATATTTTAGATATGTGAAGCTCTAAAGATAGTGACGCTCTAGTAGTATGTCTTGTTTATTTTGCAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAACGAGCAAGTCTTGCCGGAGTCTCAACCACAACTGTTTTCTACATCTTGTGTGGTTGCATCGGATATGCTGCGTTCGGCAACCAAGCCCCTGGTGACTTCCTTACCGATTTTGGTTTTTACGAACCTTATTGGCTCATCGACTTTGCCAATGCTTGCATTGCTCTCCATCTAATCGGTGCCTATCAGGTATAACTCACAAACAAAAGAATAGGATAAGTGTGTAACATACATTTACCGTGTTCAAGTTCATTAAAAGTCTCATTATTGTGTTAGAATTTTTAGCTTTAACAATTCAGAAGATTGTAGAAATGGAGTTATTACTAAATATTGTTTCTAAAAAATGCTCTTTTTTTTTTTTTATCCCTGTATTATTCGCAGGTGTATGCGCAGCCTTTTTCCAGTTTGTTGAGGAAAACTGCAACAAAAAATGGCCTCAAAGCAATTTCATCAACAAAGAATACTCGTCAAAGGTTCCTTTGCTTGGAAAATGTCGTGTCAACCTCTTCAGACTGGTTTGGAGGACATGCTATGTTGTTTTGACAACATTTGTAGCAATGATATTCCCCTTCTTCAATGCGATCTTGGGTTTGCTAGGGGCATTCGCGTTCTGGCCACTCACAGTTTATTTTCCGGTGGCAATGCACATTGCGCAGGCTAAAGTCAAGAAGTATTCTCGTAGATGGTTGGCCTTGAACCTCCTCGTATTGGTTTGCTTGATCGTCTCGGCCCTTGCCGCCGTAGGATCCATCATTGGCTTAATTAATAGTGTCAAGTCATACAAGCCCTTCAAGAATTTAGACTAGTGTGACTTATAATCTATGTTTGCCAAAAAAAAACCTGTGATCCATATGAAATTTATTTCATGCTAAATATTTAGTACTTAATGTTTCTCCAAATAATGTGACGTTCTGTTTTCAGCTATGTTAAAAAACAAAATGCTAACTTGTGTATTAGTACTAAAATTTATGAAAATGTATTAGTTATTGATTTATTTTTAGGACTACAATTATTGAATCAACATTGGATGTTTGAGTCCCATGAGATATGGATTTCAGCTTTTTTCAAATTCGTGTGGTTGTGTCAATTTCGAGTTATTATTATTTATTTTGCTTAATGGAATTGTCGGGGAAATCTTGAAAACAGACACTCACAGATTGTGTAATTTATTTGGTTTGGTGTGTCCTACATAAGTTGCTATCACATCTTATGTATTGGAGGAGTTGGGCAATAGAGGATCAAGGCAAGTTTGGTTTTCTATTAACGTTTCTACTCTGCATTTGCTTACAAAGTCATTTTCAAGGTTTTGTGGTCGTATGTCACTTGATGG RICESEQ ID NO: 9: EEC81471 Osl_24794 [Oryza sativa Indica Group] (protein);

SEQ ID NO: 10: EEC81471 Osl_24794 [Oryza sativa Indica Group] (genomic):XP_015647443.1 ATGGAGAGGCCGCAAGAGAAGGTGGCCACCACCACCACCGCCGCCTTCAACTCGCCGAGTCCGGCTACGCCGACCGCCCCGACCTCGACGACGACGGCCGCGAGAAGCGCACAGGGACGCTGGTGACGGCGAGCGCGCACATAATAACGGCGGTGATCGGCTCCGGCGTGCTGTCGCTGGCGTGGGCGATAGCGCAGCTGGGGTGGGTGATCGGGCCGGCCGTGCTGGTGGCGTTCTCGGTCATAACCTGGTTCTGCTCCAGCCTCCTCGCCGACTGCTACCGATCTCCCGACCCCGTCCATGGCAAGCGCAACTACACCTACGGCCAAGCCGTCAGGGCCAACCTAGGTGTGGCCAAGTACAGGCTCTGCTCGGTGGCACAGTACGTCAATCTCGTCGGCGTCACCATTGGCTACACCATCACTACGGCCATCAGCATGGGTGCGATCAAACGGTCCAACTGCTTCCATCGCAACGGCCACGACGCAGCCTGCTTGGCATCTGACACGACCAACATGATCATATTTGCTGGCATCCAAATCCTCCTCTCGCAGCTGCCGAATTTTCACAAAATTTGGTGGCTCTCCATTGTCGCTGCTGTCATGTCACTGGCCTACTCAACCATTGGCCTTGGCCTCTCCATTGCAAAAATTGCAGGTGGGGCCCACCCCGAGGCAACCCTCACAGGGGTGACTGTTGGAGTGGATGTGTCTGCAAGTGAGAAAATCTGGAGAACTTTTCAGTCACTTGGTGACATTGCCTTTGCATACTCCTACTCCAATGTCCTCATAGAAATTCAGGACACGCTGCGGTCGAGCCCGGCGGAGAACGAGGTGATGAAGAAGGCGTCGTTCATCGGAGTCTCGACGACGACGACGTTCTACATGCTGTGCGGCGTGCTCGGCTACGCGGCGTTCGGCAACCGCGCGCCGGGGAACTTCCTCACCGGCTTCGGCTTCTACGAGCCCTTCTGGCTCGTCGACGTCGGCAACGTCTGCATCGTCGTCCACCTCGTCGGCGCCTACCAGGTCTTCTGCCAGCCCATCTACCAGTTCGCCGAGGCCTGGGCGCGCTCGCGCGTGGCCGGACAGCGCCTTCGTCAACGGCGAGCGCGTGCTCCGGCTGCCGCTCGGCGCCGGCGACTTCCCCGTCAGCGCGCTCCGCCTCGTCTGGCGCACGGCCTACGTCGTGCTCACCGCCGTCGCCGCCATGGCGTTCCCCTTCTTCAACGACTTCCTCGGCCTCATC

CTCAGGCCAAGGTCCGGCGATTCTCGCCGACGTGGACGTGGATGAACGTGCTCAGCCTCGCCTGCCTCGTCGTCTCCCTCCTCGCCGCCGCCGGCTCCATCCAGGGCCTCATCAAATCCGTCGCACATTACAAGCCATTCAGCGTCTCCTCATGASEQ ID NO: 11: EEE66520 OsJ_22995 [Oryza sativa Japonica Group] (protein)

SEQ ID NO: 12: EEE66520 OsJ_22995 [Oryza sativa Japonica Group] (genomic)ATGGAGAGGCCGCAAGAGAAGGTGGCCACCACCACCACCGCCGCCTTCAACCTCGCCGAGTCCGGCTACGCCGACCGCCCCGACCTCGACGACGACGGCCGCGAGAAGCGCACAGGGACGCTGGTGACGGCGAGCGCGCACATAATAACGGCGGTGATCGGCTCCGGCGTGCTGTCGCTGGCGTGGGCGATAGCGCAGCTGGGGTGGGTGATCGGGCCGGCCGTGCTGGTGGCGTTCTCGGTCATAACCTGGTTCTGCTCCAGCCTCCTCGCCGACTGCTACCGATCTCCCGACCCCGTCCATGGCAAGCGCAACTACACCTACGGCCAAGCCGTCAGGGCCAACCTAGGTGTGGCCAAGTACAGGCTCTGCTCGGTGGCACAGTACGTCAATCTCGTCGGCGTCACCATTGGCTACACCATCACTACGGCCATCAGCATGGGTGCGATCAAACGGTCCAACTGGTTCCATCGCAACGGCCACGACGCAGCCTGCTTGGCATCTGACACGACCAACATGATCATATTTGCTGGCATCCAAATCCTCCTCTCGCAGCTGCCGAATTTTCACAAAATTTGGTGGCTCTCCATTGTCGCTGCTGTCATGTCACTGGCCTACTCAACCATTGGCCTTGGCCTCTCCATTGCAAAAATTGCAGGTGGGGCCCACCCCGAGGCAACCCTCACAGGGGTGACTGTTGGAGTGGATGTGTCTGCAAGTGAGAAAATCTGGAGAACTTTTCAGTCACTTGGTGACATTGCCTTTGCATACTCCTACTCCAATGTCCTCATAGAAATTCAGGACACGCTGCGGTCGAGCCCGGCGGAGAACGAGGTGATGAAGAAGGCGTCGTTCATCGGAGTCTCGACGACGACGACGTTCTACATGCTGTGCGGCGTGCTCGGCTACGCGGCGTTCGGCAACCGCGCGCCGGGGAACTTCCTCACCGGCTTCGGCTTCTACGAGCCCTTCTGGCTCGTCGACGTCGGCAACGTCTGCATCGTCGTCCACCTCGTCGGCGCCTACCAGGTCTTCTGCCAGCCCATCTACCAGTTCGCCGAGGCCTGGGCGCGCTCGCGGTGGCCGGACAGCGCCTTCGTCAACGGCGAGCGCGTGCTCCGGCTGCCGCTCGGCGCCGGCGACTTCCCCGTCAGCGCGCTCCGCCTCGTCTGGCGCACGGCCTACGTCGTGCTCACCGCCGTCGCCGCCATGGCGTTCCCCCTTCTTCAACGACTTCCTCGGCCTCATC

CTCAGGCCAAGGTCCGGCGATTCTCGCCGACGTGGACGTGGATGAACGTGCTCAGCCTCGCCTGCCTCGTCGTCTCCCTCCTCGCCGCCGCCGGCTCCATCCAGGGCCTCATCAAATCCGTCGCACATTACAAGCCATTCAGCGTCTCCTCATGASEQ ID NO: 13: XP_015647443 AAP6 [Oryza sativa Japonica Group] (protein)MGMERPQEKVATTTTTAAFNLAESGYADRPDLDDDGREKRTGTLVTASAHIITAVIGSGVLSLAWAIAQLGWVIGPAVLVAFSVITWFCSSLLADCYRSPDPVHGKRNYTYGQAVRANLGVAKYRLCSVAQYYVNLVGVTIGYTITTAISMGAIKRSNWFHRNGHDAACLASDTTNMIIFAGIQILLSQLPNFHKIWWLSIVAAVMSLAYSTIGLGLSIAKIAGGAHPEATLTGVTVGVDVSASEKIWRTFQSLGDIAFAYSYSNVLIEIQDTLRSSPAENEVMKKASFIGVSTTTTFYMLCGVLGYAAFGNRAPGNFLTGFGFYEPFWLVDVGNVCIVVHLVGAYQFCQPIYQFAEAWARSRWPDSAFVNGERVLRLPLGAGDFPVSALRLVWRTAYVVLTAVAAMAF

AAGSIQGLIKSVAHYKPFSVSSSEQ ID NO: 14: XP_015647443 AAP6 [Oryza sativa Japonica Group] (genomic)ATGGGGATGGAGAGGCCGCAAGAGAAGGTGGCCACCACCACCACCGCCGCCTTCAACCTCGCCGAGTCCGGCTACGCCGACCGCCCCGACCTCGACGACGACGGCCGCGAGAAGCGCACAGGGACGCTGGTGACGGCGAGCGCGCACATAATAACGGCGGTGATCGGCTCCGGCGTGCTGTCGCTGGCGTGGGCGATAGCGCAGCTGGGGTGGGTGATCGGGCCGGCCGTGCTGGTGGCGTTCTCGGTCATAACCTGGTTCTGCTCCAGCCTCCTCGCCGACTGCTACCGATCTCCCGACCCCGTCCATGGCAAGCGCAACTACACCTACGGCCAAGCCGTCAGGGCCAACCTAGGTGTGGCCAAGTACAGGCTCTGCTCGGTGGCACAGTACGTCAATCTCGTCGGCGTCACCATTGGCTACACCATCACTACGGCCATCAGCATGGGTGCGATCAAACGGTCCAACTGGTTCCATCGCAACGGCCACGACGCAGCCTGCTTGGCATCTGACACGACCAACATGATCATATTTGCTGGCATCCAAATCCTCCTCTCGCAGCTGCCGAATTTTCACAAAATTTGGTGGCTCTCCATTGTCGCTGCTGTCATGTCACTGGCCTACTCAACCATTGGCCTTGGCCTCTCCATTGCAAAAATTGCAGGTGGGGCCCACCCCGAGGCAACCCTCACAGGGGTGACTGTTGGAGTGGATGTGTCTGCAAGTGAGAAAATCTGGAGAACTTTTCAGTCACTTGGTGACATTGCCTTTGCATACTCCTACTCCAATGTCCTCATAGAAATTCAGGACACGCTGCGGTCGAGCCCGGCGGAGAACGAGGTGATGAAGAAGGCGTCGTTCATCGGAGTCTCGACGACGACGACGTTCTACATGCTGTGCGGCGTGCTCGGCTACGCGGCGTTCGGCAACCGCGCGCCGGGGAACTTCCTCACCGGCTTCGGCTTCTACGAGCCCTTCTGGCTCGTCGACGTCGGCAACGTCTGCATCGTCGTCCACCTCGTCGGCGCCTACCAGGTCTTCTGCCAGCCCATCTACCAGTTCGCCGAGGCCTGGGCGCGCTCGCGGTGGCCGGACAGCGCCTTCGTCAACGGCGAGCGCGTGCTCCGGCTGCCGCTCGGCGCCGGCGACTTCCCCGTCAGCGCGCTCCGCCTCGTCTGGCGCACGGCCTACGTCGTGCTCACCGCCGTCGCCGCCATGGCGTTCCCCTTCTTCAACGACTTCCTCG

GTACATGTCTCAGGCCAAGGTCCGGCGATTCTCGCCGACGTGGACGTGGATGAACGTGCTCAGCCTCGCCTGCCTCGTCGTCTCCCTCCTCGCCGCCGCCGGCTCCATCCAGGGCCTCATCAAATCCGTCGCACATTACAAGCCATTCAGCGTCTCCTCATGASEQ ID NO: 15: BAC82953.1 putative amino acid permease [Oryza sativa JaponicaGroup] (protein)MAAAGRTLGCIYAGTLVTASAHIITAVIGSGVLSLAWAIAQLGWVIGPAVLVAFSVITWFCSSLLADCYRSPDPVHGKRNYTYGQAVRANLGVAKYRLCSVAQYVNLVGVTIGYTITTAISMGAIKRSNWFHRNGHDAACLASDTTNMIIFAGIQILLSQLPNFHKIWWLSIVAAVMSLAYSTIGLGLSIAKIAGGAHPEATLTGVTVGVDVSASEKIWRTFQSLGDIAFAYSYSNVLIEIQDTLRSSPAENEVMKKASFIGVSTTTTFYMLCGVLGYAAFGNRAPGNFLTGFGFYEPFWLVDVGNVCIVVHLVGAYQVFCQPIYQFAEAWARSRWPDSAFVNGERVLRL

YMSQAKVRRFSPTWTWMNVLSLACLVVSLLAAAGSIQGLIKSVAHYKPFSVSSSEQ ID NO: 16: BAC82953.1 putative amino acid permease [Oryza sativa JaponicaGroup] (genomic) ATGGCGGCGGCCGGACGAACACTTGGATGCATATATGCAGGGACGCTGGTGACGGCGAGCGCGCACATAATAACGGCGGTGATCGGCTCCGGCGTGCTGTCGCTGGCGTGGGCGATAGCGCAGCTGGGGTGGGTGATCGGGCCGGCCGTGCTGGTGGCGTTCTCGGTCATAACCTGGTTCTGCTCCAGCCTCCTCGCCGACTGCTACCGATCTCCCGACCCCGTCCATGGCAAGCGCAACTACACCTACGGCCAAGCCGTCAGGGCCAACCTAGGTGTGGCCAAGTACAGGCTCTGCTCGGTGGCACAGTACGTCAATCTCGTCGGCGTCACCATTGGCTACACCATCACTACGGCCATCAGCATGGGTGCGATCAAACGGTCCAACTGGTTCCATCGCAACGGCCACGACGCAGCCTGCTTGGCATCTGACACGACCAACATGATCATATTTGCTGGCATCCAAATCCTCCTCTCGCAGCTGCCGAATTTTCACAAAATTTGGTGGCTCTCCATTGTCCGCTGCTGTCATGTCACTGGCCTACTCAACCATTGGCCTTGGCCTCTCCATTGCAAAAATTGCAGGTGGGGCCCACCCCGAGGCAACCCTCACAGGGGTGACTGTTGGAGTGGATGTGTCTGCAAGTGAGAAAATCTGGAGAACTTTTCAGTCACTTGGTGACATTGCCTTTGCATACTCCTACTCCAATGTCCTCATAGAAATTCAGGACACGCTGCGGTCGAGCCCGGCGGAGAACGAGGTGATGAAGAAGGCGTCGTTCATCGGAGTCTCGACGACGACGACGTTCTACATGCTGTGCGGCGTGCTCGGCTACGCGGCGTTCGGCAACCGCGCGCCGGGGAACTTCCTCACCGGCTTCGGCTTCTACGAGCCCTTCTGGCTCGTCGACGTCGGCAACGTCTGCATCGTCGTCCACCTCGTCGGCGCCTACCAGGTCTTCTGCCAGCCCATCTACCAGTTCGCCGAGGCCTGGGCGCGCTCGCGGTGGCCGGACAGCGCCTTCGTCAACGGCGAGCGCGTGCTCCGGCTGCCGCTCGGCGCCGGCGACTTCCCCGTCAGCGCGCTCCGCCTCGTCTGGCGCACGGCCTACGTCGTGCTCACCGCCGTCGCCGCCATGGC

CTCACCGTCTACTTCCCCGTCCAGATGTACATGTCTCAGGCCAAGGTCCGGCGATTCTCGCCGACGTGGACGTGGATGAACGTGCTCAGCCTCGCCTGCCTCGTCGTCTCCCTCCTCGCCGCCGCCGGCTCCATCCAGGGCCTCATCAAATCCGTCGCACATTACAAGCCATTCAGCGTCTCCTCATGASEQ ID NO: 17: XP_015644123.1 amino acid permease 3 [Oryza sativa JaponicaGroup] (protein) MAKDVEMAVRNGDGGGGGGYYATHPHGGAGGEDVDDDGKQRRTGNVWTASAHIITAVIGSGVLSLAWATAQLGWVVGPVTLMLFALITYYTSGLLADCYRTGDPVSGKRNYTYMDAVAAYLGGWQVWSCGVFQYVNLVGTAIGYTITASISAAAVHKANCYHKNGHDADCGVYDTTMIVFGVVQIFFSMLPNFSDLSWLSILAAVMSFSYSTIAVGLSLARTISGATGKTTLTGVEVGVDVTSAQKIWLAFQALGDIAFAYSYSMILIEIQDTVKSPPAENKTMKKATLLGVSTTTAFYMLCGCLGYAAFGNAAPGNMLTGFGFYEPYWLIDFANVCIVVHLVGAYQVFCQPIFAAVETFAARRWPGSEFITRERPVVAGRSFSVNMFRLTWRTAFVVVSTVL

ASAVASIEGVSESLKHYVPFKTKSSEQ ID NO: 18: XP_015644123.1 amino acid permease 3 [Oryza sativa JaponicaGroup] (genomic) ATGGCGAAGGACGTGGAGATGGCGGTGCGGAACGGAGACGGCGGCGGCGGCGGCGGCTACTACGCCACCCACCCGCACGGCGGCGCCGGCGGCGAGGACGTCGACGACGACGGCAAGCAGCGGCGAACCGGTAACGTATGGACGGCGAGCGCGCACATCATCACGGCGGTGATCGGCTCCGGCGTGCTCTCTCTCGCATGGGCAACGGCGCAGCTCGGCTGGGTGGTCGGGCCGGTGACTCTGATGCTCTTCGCCCTCATCACGTACTACACCTCTGGGCTCCTCGCCGACTGCTACCGCACTGGCGATCCGGTCAGCGGCAAGCGCAACTACACCTACATGGATGCCGTTGCGGCCTACTTAGGTGGCTGGCAAGTCTGGTCCTGTGGTGTTTTCCAATATGTCAACCTGGTTGGGACAGCAATTGGGTACACAATCACAGCATCCATCAGCGCAGCGGCTGTGCACAAGGCCAACTGCTACCACAAGAACGGCCACGATGCCGATTGCGGTGTCTACGACACCACGTACATGATCGTCTTTGGAGTCGTCCAGATCTTCTTCTCCATGCTGCCCAACTTCAGTGACCTCTCATGGCTTTCCATCCTCGCCGCGGTCATGTCATTCTCATACTCGACCATTGCCGTTGGCCTCTCGCTTGCGCGAACAATATCAGGTGCTACTGGTAAGACTACTCTGACTGGCGTTGAGGTTGGAGTTGACGTCACTTCAGCCCAGAAGATCTGGCTCGCGTTCCAAGCGCTCGGTGACATCGCGTTCGCCTACTCCTACTCCATGATCCTTATAGAAATTCAGGACACGGTGAAGTCTCCACCGGCGGAGAACAAGACGATGAAGAAGGCAACGCTGCTGGGGGTGCGACCACGACGGCGTTCTACATGCTGTGCGGGTGCCTGGGGTACGCGGCGTTCGGGAACGCGGCGCCGGGGAACATGCTCACCGGGTTCGGCTTCTACGAGCCCTACTGGCTGATCGACTTCGCCAACGTCTGCATCGTGGTCCACCTGGTCGGCGCCTACCAGGTGTTCTGCCAGCCCATCTTCGCCGCCGTCGAGACGTTCGCCGCCAGGCGGTGGCCGGGCTCGGAGTTCATCACCCGGGAGCGCCCCGTCGTGGCCGGCAGGTCGTTCAGCGTCAACATGTTCAGGCTGACGTGGCGGACGGCGTTCGTGGTCGTCAGCACGGTGCTCGCCATCGTGATGCCCTTCTTCAACGACATC

GAGATGTACATCCGGCAGCGGCGGATACAGCGGTACACGTCCAGGTGGGTGGCGCTGCAGACGCTCAGCCTCCTCTGCTTCCTCGTCTCGCTCGCCTCCGCCGTCGCCTCCATCGAGGGCGTCAGCGAGTCGCTCAAGCACTACGTCCCCTTCAAGACCAA GTCGTGASEQ ID NO: 19: EEC68963.1 hypothetical protein Osl_37697 [Oryza sativa IndicaGroup] (protein)MSDMASGQKAKQQVMKPMEVSVEAGNAGDAAWLDDDGRARRTGTFWTASAHIITVIGSGVLSLAWAIAQLGWVAGPAVMLLFAFVIYYTSTLLAECYRTGDPATGKRNYTYMDAVRANLGGAKVTFCGVIQYANLVGVAIGYTIASSISMRAIRRAGCFHHNGHGDPCRSSSNPYMILFGVVQIVFSQIPDFDQIWWLSIVAAVMSFTYSGIGLSLGIVQTISNGGIQGSLTGISIGVGVSSTQKVWRSLQAFGDIAFAYSFSNILIEIQDTIKAPPPSEAKVMKSATRLSVATTTVFYMLCGCMGYAAFGDAAPDNLLTGFGFYEPFWLLDVANVAIVVHLVGAYQVFVQPIFAFVERWASRRWPDSAFIAKELRVGPFALSLFRLTWRSAFVCLTTVVAMLLPF

GSIADVIDALKVYRPFSGSEQ ID NO: 20: EEC68963.1 hyptothetical protein Osl_37697 [Oryza sativa IndicaGroup] (genomic) ATGTCCGACATGGCGTCGGGGCAGAAGGCGAAGCAGCAGGTGATGAAGCCGATGGAGGTGTCGGTGGAGGCCGGGAACGCCGGGGATGCGGCGTGGCTGGACGACGACGGGCGGGCGCGGCGGACGGGCACGTTCTGGACGGCGAGCGCGCACATCATCACCGCCGTCATCGGCTCCGGCGTGCTGTCGCTGGCGTGGGCGATCGCGCAGCTCGGGTGGGTGGCCGGCCCCGCCGTGATGCTCCTCTTCGCCTTCGTCATCTACTACACCTCCACCCTCCTCGCCGAGTGCTACCGCACCGGCGACCCGGCCACCGGCAAGCGCAACTACACCTACATGGACGCCGTGCGCGCCAACCTCGGCGGCGCCAAGGTCACCTTCTGCGGCGTCATCCAGTACGCCAACCTCGTCGGCGTCGCCATCGGCTACACCATCGCGTCGTCCATCAGCATGCGCGCCATCAGGAGGGCCGGCTGCTTCCACCACAACGGCCATGGTGACCCGTGCCGCAGCTCCAGCAACCCTTACATGATCCTCTTCGGCGTCGTGCAGATCGTCTTCTCGCAGATCCCGGACTTCGACCAGATTTGGTGGCTGTCCATCGTCGCCGCCGTCATGTCCTTCACCTACTCCGGCATCGGCCTCTCCCTCGGCATCGTCCAGACAATCTCCAATGGCGGGATCCAGGGCAGCCTCACCGGAATCAGCATCGGCGTCGGCGTCAGCTCAACGCAGAAGGTGTGGCGCAGCCTGCAGGCATTCGGCGACATCGCCTTCGCATACTCCTTCTCCAACATCCTCATCGAGATCCAAGACACGATCAAGGCGCCGCCGCCGTCGGAGGCGAAGGTGATGAAGAGCGCGACGAGGCTGAGCGTGGCGACGACCACGGTGTTCTACATGCTGTGCGGGTGCATGGGCTACGCGGCGTTCGGCGACGCGGCGCCCGACAACCTCCTCACGGGCTTCGGCTTCTACGAGCCCTTCTGGCTGCTCGACGTCGCCAACGTCGCCATCGTCGTGCACCTCGTCGGCGCCTACCAGGTGTTCGTCCAGCCAATCTTCGCCTTCGTCGAGCGCTGGGCCTCCCGCCGGTGGCCGGACAGCGCGTTCATCGCCAAGGAGCTCCGCGTGGGGCCCTTCGCGCTCAGCCTCTTCCGCCTGACGTGGCGCTCGGCGTTCGTCTGCCTCACCACAGTCGTCGCCATGCTCCTCCCCTTCTTCGGCAACGTGGTGG

GTACATCGCGCAGCGCGGCGTGCCACGTGGCAGCGCGAGGTGGGTCTCGCTCAAGACGCTCAGCGCGTGCTGCCTCGTCGTCTCCATCGCCGCCGCCGCGGGCTCCATTGCTGACGTCATCGACGCTCTCAAGGTGTACAGACCGTTCAGCGGATGASEQ ID NO: 21: EAY82481.1 hypothetical protein Osl_37698 [Oryza sativa IndicaGroup] (protein)MASGQKVVKPMEVSVEAGNAGEAAWLDDDGRARRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWVAGPAVMLLFAFVIYYTSTLLAECYRTGDPATGKRNYTYMDAVRANLGGAKVTFCGVIQYANLVGVAIGYTIASSISMRAIRRAGCFHHNGHGDPCRSSSNPYMILFGVVQIVFSQPIDFDQIWWLSIVAAVMSFTYSGIGLSLGIVQTISNGGIQGSLTGISIGVGVSSTQKVWRSLQAFGDIAFAYSFSNILIEIQDTIKAPPPSEAKVMKSATRLSVATTTVFYMLCGCMGYAAFGDAAPDNLLTGFGFYEPFWLLDVANVAIVVHLVGAYQVFVQPIFAFVERWASRRWPDSAFIAKELRVGPFALSLFRLTWRSAFVCLTTVVAMLLPFFGNVVGLLG

VYRPFSG SEQ ID NO: 22: EAY82481.1 hypothetical protein Osl_37698 [Oryza sativa Indica Group] (genomic)ATGGCGTCGGGGCAGAAGGTGGTGAAGCCGATGGAGGTGTCGGTGGAGGCCGGGAACGCCGGGGAGGCGGCGTGGCTGGACGACGACGGGCGGGCGCGGCGGACGGGCACGTTCTGGACGGCGAGCGCGCACATCATCACCGCCGTCATCGGCTCCGGCGTGCTGTCGCTGGCGTGGGCGATCGCGCAGCTGGGCTGGGTGGCCGGCCCCGCCGTGATGCTCCTCTTCGCCTTCGTCATCTACTACACCTCCACCCTCCTCGCCGAGTGCTACCGCACCGGCGACCCGGCCACCGGCAAGCGCAACTACACCTACATGGACGCCGTGCGCGCCAACCTCGGCGGCGCCAAGGTCACCTTCTGCGGCGTCATCCAGTACGCCAACCTCGTCGGCGTCGCCATCGGCTACACCATCGCGTCGTCCATCAGCATGCGCGCCATCAGGAGGGCCGGCTGCTTCCACCACAACGGCCATGGTGACCCGTGCCGCAGCTCCAGCAACCCTTACATGATCCTCTTCGGCGTCGTGCAGATCGTCTTCTCCCAGATCCCTGACTTCGACCAGATTTGGTGGCTGTCCATCGTCGCCGCCGTCATGTCCTTCACCTACTCCGGCATCGGCCTCTCCCTCGGCATCGTCCAGACTATCTCCAATGGCGGGATCCAGGGCAGCCTCACCGGCATCAGCATCGGAGTCGGCGTCAGCTCGACGCAGAAGGTGTGGCGCAGCTTGCAGGCATTCGGCGACATCGCCTTCGCATACTCCTTCTCCAACATCCTCATCGAGATCCAAGACACGATCAAGGCGCCGCCGCCGTCGGAGGCGAAGGTGATGAAGAGCGCGACGAGGCTGAGCGTGGCGACGACCACGGTGTTCTACATGCTGTGCGGGTGCATGGGCTACGCGGCGTTCGGCGACGCGGCGCCCGACAACCTCCTCACCGGCTTCGGGTTCTACGAGCCCTTCTGGCTGCTCGACGTCGCCAACGTCGCCATCGTCGTGCACCTCGTCGGCGCCTACCAGGTGTTCGTCCAGCCAATCTTCGCCTTCGTCGAGCGCTGGGCCTCCCGCCGGTGGCCGGACAGCGCGTTCATCGCCAAGGAGCTCCGCGTGGGGCCCTTCGCGCTCAGCCTCTTCCGCCTGACGTGGCGCTCGGCGTTCGTCTGCCTCACCACCGTCGTCGCC

GGCCGCTCACCGTCTACTTCCCCGTCGAGATGTACATCGCGCAGCGTGGCGTGCCGCGGGGGAGCGCGAGGTGGATCTCCCTCAAGACGCTCAGCGCGTGCTGCCTAGTCGTCTCCATCGCCGCCGCGGCGGGCTCCATTGCTGACGTCATCGACGCGCTCAAGGTGTACAGACCCTTCAGCGGATGASEQ ID NO: 23: BAD53557 putative amino acid carrier [Oryza sativa Japonica Group] (protein)MDVYLPRTQGDVDDDGKERRTGTVWTATAHIITAVIGSGVLSLAWAMAQLGWVAGPITLLLFAAITFYTCGLLSDCYRVGDPATGKRNYTYTDAVKSYLGGWHVWFCGFCQYVNMFGTGIGYTITASISAAAINKSNCYHWRGHGTDCSQNTSAYIIGFGVLQALFCQLPNFHQLWWLSIIAAVMSFSYAAIAVGLSLAQTIMDPLGRTTLTGTVVGVDVDATQKVWLTFQALGNVAFAYSYAIILIEIQDTLRSPPPENATMRRATAAGISTTTGFYLLCGCLGYSAFGNAAPGNILTGFGFYEPYWLVDVANACIVVHLVGGFQVFCQPLFAAVEGGVARRCPGLL

RQRQLPRFSAKWWVALQSLSLVCFLVTVAACAASIQGVLDSLKTYVPFKTRSSEQ ID NO: 24: BAD53557 putative amino acid carrier [Oryza sativa Japonica Group] (genomic)ATGGACGTCTACCTTCCCCGGACCCAAGGCGACGTCGACGACGACGGCAAGGAGAGGAGGACAGGGACGGTGTGGACGGCGACGGCGCACATAATCACGGCGGTGATCGGGTCCGGCGTGCTGTCGCTGGCGTGGGCGATGGCGCAGCTGGGGTGGGTGGCTGGCCCCATCACCCTCCTCCTCTTCGCCGCCATCACCTTCTACACCTGCGGCCTCCTCTCCGACTGCTACCGCGTCGGCGACCCGGCCACCGGCAAGCGCAACTACACCTACACCGACGCCGTCAAGTCCTACCTCGGTGGCTGGCACGTCTGGTTCTGCGGCTTCTGCCAGTACGTCAACATGTTCGGCACCGGCATCGGCTACACCATCACCGCCTCCATCTCCGCCGCGGCTATCAACAAGTCCAACTGCTACCACTGGCGCGGCCATGGCACGGACTGCAGCCAGAACACGAGCGCCTACATCATCGGCTTCGGCGTCCTGCAGGCCCTCTTCTGCCAGCTCCCAAACTTCCACCAGCTCTGGTGGCTGTCCATCATCGCCGCCGTCATGTCCTTCTCGTACGCCGCCATCGCCGTCGGCTTGTCGCTGGCGCAGACCATCATGGACCCGCTGGGGAGGACGACGCTGACGGGCACGGTGGTCGGCGTCGACGTCGACGCCACGCAGAAGGTGTGGCTCACGTTCCAGGCGCTGGGGAACGTCGCCTTCGCCTACTCCTACGCCATCATCCTCATCGAGATCCAGGACACGCTGCGGTCGCCGCCGCCGGAGAACGCGACGATGCGGCGCGCCACGGCGGCGGGGATCTCGACGACCACGGGGTTCTACCTGCTGTGCGGCTGCCTGGGCTACTCGGCGTTCGGGAACGCGGCGCCGGGCAACATCCTCACCGGCTTCGGCTTCTACGAGCCATACTGGCTGGTGGACGTGGCCAACGCCTGCATCGTGGTGCACCTCGTCGGCGGGTTCCAGGTGTTCTGCCAGCCGCTGTTCGCCGCCGTGGAGGGCGGCGTGGCGCGGCGGTGCCCGGGGCTGCTCGGCGGCGGCGCGGGGCGGGCCAGCGGCGTGAACGTGTTCCGGCTTGTGTGGAGGACGGCGTTCGTGGCGGTGATCACGCTGCT

GCTGCCGCGGTTCAGCGCCAAGTGGGTGGCGCTGCAGAGCCTGAGCCTCGTCTGCTTCCTCGTCACCGTCGCCGCCTGCGCCGCCTCCATCCAGGGCGTCCTCGACTCGCTCAAGACCTACGTGCCCTTCAAGACCAGGTCCTGASEQ ID NO: 25: XP_015637472.1 AAP3 isoform X2 [Oryza sativa Japonica Group] (protein)MGENGVVASKLCYPAAAMEVVAAELGHTAGSKLYDDDGRLKRTGTMWTASAHIITAVIGSGVLSLGWAIAQLGWVAGPAVMLLFSFVTYYTSALLADCYRSGDESTGKRNYTYMDAVNANLSGIKVQVCGFLQYANIVGVAIGYTIAASISMLAIKRANCFHVEGHGDPCNISSTPYMIIFGVAEIFFSQIPDFDQISWLSILAAVMSFTYSTIGLGLGVVQVVANGGVKGSLTGISIGVVTPMDKVWRSLQAFGDIAFAYSYSLILIEIQDTIRAPPPSESRVMRRATVVSVAVTTLFYMLCGCTGYAAFGDAAPGNLLTGFGFYEPFWLLDVANAAIVVHLVGAYQVYCQPLFAFVEKWAQQRWPKSWYITKDIDVPLSLSGGGGGGGRCYKLNLFRLTWRSAF

SLACLAITVASAAGSIAGILSDLKVYKPFATTYSEQ ID NO: 26: XP_015637472.1 AAP3 isoform X2 [Oryza sativa Japonica Group] (genomic)ATGGGGGAGAACGGTGTGGTGGCGAGCAAGCTGTGCTACCCGGCGGCGGCCATGGAGGTGGTCGCCGCCGAGCTCGGCCACACGGCCGGCTCCAAGCTGTACGACGACGACGGCCGCCTCAAGCGCACCGGGACGATGTGGACGGCGAGCGCGCACATCATCACGGCGGTGATCGGCTCCGGCGTGCTGTCGCTGGGGTGGGCGATCGCGCAGCTGGGTTGGGTGGCCGGCCCCGCCGTCATGCTGCTCTTCTCGTTCGTCACCTACTACACCTCCGCGCTGCTCGCCGACTGCTACCGCTCCGGCGACGAGAGCACCGGCAAGCGCAACTACACCTACATGGACGCCGTGAACGCCAACCTGAGTGGCATCAAGGTCCAGGTCTGCGGGTTCCTGCAGTACGCCAACATCGTCGGCGTCGCCATCGGCTACACCATTGCCGCCTCCATTAGCATGCTGGCGATCAAGCGGGCGAACTGCTTCCACGTCGAGGGGCACGGCGACCCGTGCAACATCTCGAGCACGCCGTACATGATCATCTTCGGCGTGGCGGAGATCTTCTTCTCGCAGATCCCGGACTTCGACCAGATCTCGTGGCTGTCCATCCTCGCCGCCGTCATGTCGTTCACCTACTCCACCATCGGGCTCGGCCTCGGCGTCGTGCAGGTGGTGGCCAACGGCGGCGTCAAGGGGAGCCTCACCGGGATCAGCATCGGCGTGGTGACGCCCATGGACAAGGTGTGGCGGAGCCTGCAGGCGTTCGGCGACATCGCCTTCGCCTACTCCTACTCCCTCATCCTCATCGAGATCCAGGACACCATCCGGGCGCCGCCGCCGTCGGAGTCGAGGGTGATGCGGCGCGCCACCGTGGTGAGCGTCGCCGTCACCACGCTCTTCTACATGCTCTGCGGCTGCACGGGGTACGCGGCGTTCGGCGACGCCGCGCCGGGCAACCTCCTCACCGGGTTCGGCTTCTACGAGCCCTTCTGGCTCCTCGACGTTGCCAACGCCGCCATCGTCGTCCACCTCGTCGGCGCCTACCAGGTCTACTGCCAGCCGCTGTTCGCCTTCGTCGAGAAGTGGGCGCAGCAGCGGTGGCCGAAATCATGGTACATCACCAAGGATATCGACGTGCCGCTCTCCCTCTCCGGCGGCGGCGGCGGCGGCGGAAGGTGCTACAAGCTGAACCTGTTCAGGCTGACATGGAGGTCGGCGTTCGTGGTGGCGACGACGGTGG

GGATACCGAGGTGGAGCACGCGGTGGGTGTGCCTGCAGCTGCTCAGCCTCGCCTGCCTCGCCATCACCGTCGCCTCCGCCGCCGGCTCCATCGCCGGAATCCTCTCCGACCTCAAGGTCTACAAGCCGTTCGCCACCACCTACTAASEQ ID NO: 27: XP_025881587 AAP3 isoform X1 [Oryza sativa Japonica Group] (protein)MTHHTKFNPNYISICNPASSLSLIFTSLFLNWKRVRGSRRGDFCKEMGENGVVASKLCYPAAAMEVVAAELGHTAGSKLYDDDGRLKRTGTMWTASAHIITAVIGSGVLSLGWAIAQLGWVAGPAVMLLFSFVTYYTSALLADCYRSGDESTGKRNYTYMDAVNANLSGIKVQVCGFLQYANIVGVAIGYTIAASISMLAIKRANCFHVEGHGDPCNISSTPYMIIFGVAEIFFSQIPDFDQISWLSILAAVMSFTYSTIGLGLGVVQVVANGGVKGSLTGISIGVVTPMDKVWRSLQAFGDIAFAYSYSLILIEIQDTIRAPPPSESRVMRRATVVSVAVTTLFYMLCGCTGYAAFGDAAPGNLLTGFGFYEPFWLLDVANAAIVVHLVGAYQVYCQPLFAFVEKWAQQRWPKSWWYITKDIDVPLSLSGGGGGGGRCYKLNLFRLTWRSAFVVATTVVSMLLP

GSIAGILSDLKVYKPFATTYSEQ ID NO: 28: XP_025881587 AAP3 X1 [Oryza sativa Japonica Group](genomic) ATGACACACCACACCAAGTTCAACCCCAACTATATCTCTATTTGTAACCCTGCTTCTTCTCTCTCTTTGATCTTCACTTCTCTCTTCCTCAATTGGAAGAGGGTTAGGGATCAAGAAGAGGAGACTTTTGCAAAGAGATGGGGGAGAACGGTGTGGTGGCGAGCAAGCTGTGCTACCCGGCGGCGGCCATGGAGGTGGTCGCCGCCGAGCTCGGCCACACCGGCCGGCTCCAAGCTGTACGACGACGACGGCCGCCTCAAGCGCACCGGGACGATGTGGACGGCGAGCGCGCACATCATCACGGCGGTGATCGGCTCCGGCGTGCTGTCGCTGGGGTGGGCGATCGCGCAGCTGGGTTGGGTGGCCGGCCCCGCCGTCATGCTGCTCTTCTCGTTCGTCACCTACTACACCTCCGCGCTGCTCGCCGACTGCTACCGCTCCGGCGACGAGAGCACCGGCAAGCGCAACTACACCTACATGGACGCCGTGAACGCCAACCTGAGTGGCATCAAGGTCCAGGTCTGCGGGTTCCTGCAGTACGCCAACATCGTCGGCGTCGCCATCGGCTACACCATTGCCGCCTCCATTAGCATGCTGGCGATCAAGCGGGCGAACTGCTTCCACGTCGAGGGGCACGGCGACCCGTGCAACATCTCGAGCACGCCGTACATGATCATCTTCGGCGTGGCGGAGATCTTCTTCTCGCAGATCCCGGACTTCGACCAGATCTCGTGGCTGTCCATCCTCGCCGCCGTCATGTCGTTCACCTACTCCACCATCGGGCTCGGCCTCGGCGTCGTGCAGGTGGTGGCCAACGGCGGCGTCAAGGGGAGCCTCACCGGGATCAGCATCGGCGTGGTGACGCCCATGGACAAGGTGTGGCGGAGCCTGCAGGCGTTCGGCGACATCGCCTTCGCCTACTCCTACTCCCTCATCCTCATCGAGATCCAGGACACCATCCGGGCGCCGCCGCCGTCGGAGTCGAGGGTGATGCGGCGCGCCACCGTGGTGAGCGTCGCCGTCACCACGCTCTTCTACATGCTCTGCGGCTGCACGGGGTACGCGGCGTTCGGCGACGCCGCGCCGGGCAACCTCCTCACCGGGTTCGGCTTCTACGAGCCCTTCTGGCTCCTCGACGTTGCCAACGCCGCCATCGTCGTCCACCTCGTCGGCGCCTACCAGGTCTACTGCCAGCCGCTGTTCGCCTTCGTCGAGAAGTGGGCGCAGCAGCGGTGGCCGAAATCATGGTACATCACCAAGGATATCGACGTGCCGCTCTCCCTCTCCGGCGGCGGCGGCGGCGGCGGAAGGTGCTACAAGCTGAACCTGTTCAGGCTGACATGGAGGTCGGCGTTCGTGGTGGCGACGACGGTGGTGTCGATGCTGCTGCCGTTCTTCAACGAC

GTGGAGATGTACATCGTGCAGAAGAGGATACCGAGGTGGAGCACGCGGTGGGTGTGCCTGCAGCTGCTCAGCCTCGCCTGCCTCGCCATCACCGTCGCCTCCGCCGCCGGCTCCATCGCCGGAATCCTCTCCGACCTCAAGGTCTACAAGCCGTTCGCCACCA CCTACTAASEQ ID NO: 29: XP_015629427 AAP4 [Oryza sativa Japonica Group] (protein)MGENVVGTYYYPPSAAAMDGVELGHAAAGSKLFDDDGRPRRNGTMWWTASAHIITAVIGSGVLSLGWAIAQLGWVAGPAVMVLFSLVTYYTSSLLSDCYRSGDPVTGKRNYTYMDAVNANLSGFKVKICGFLQYANIVGVAIGYTIAASISMLAIGRANCFHRKGHGDPCNVSSVPYMIVFGVAEVFFSQIPDFDQISWLSMLAAVMSFTYSVIGLSLGIVQVVANGGLKGSLTGISIGVVTPMDKVWRSLQAFGDIAFAYSYSLILIEIQDTIRAPPPSESAVMKRATVVSVAVTTVFMLCGSMGYAAFGDDAPGNLLTGFGFYEPFWLLDIANAAIVVHLVGAYQVFCQPLFAFVEKWAAQRWPESPYITGEVELRLSPSSRRCRVNLFRSTWRTAFVVATTVV

ISIAAAAGSIAGVMSDLKVYRPFKGYSEQ ID NO: 30: XP_015629427 AAP4 [Oryza sativa Japonica Group] (genomic)ATGGGGGAGAACGTGGTTGGCACGTACTACTACCCGCCTTCGGCGGCCGCCATGGACGGCGTGGAGCTCGGCCACGCCGCCGCCGGCTCCAAGCTCTTCGACGACGACGGCCGCCCCAGGCGCAACGGGACGATGTGGACGGCGAGCGCGCACATCATCACGGCGGTGATCGGCTCCGGCGTGCTGTCGCTGGGGTGGGCCATCGCGCAGCTCGGCTGGGTGGCCGGGCCGGCGGTCATGGTGCTCTTCTCCCTCGTCACCTACTACACCTCATCCCTCCTCTCCGATTGCTACCGCTCCGGCGACCCCGTCACCGGCAAGCGGAACTACACCTACATGGACGCCGTGAACGCCAACCTGAGCGGGTTCAAGGTGAAGATCTGCGGGTTCTTGCAGTACGCCAACATCGTCGGCGTCGCCATCGGCTACACCATCGCGGCGTCCATCAGCATGCTGGCGATCGGGAGGGCCAACTGCTTCCACAGGAAGGGGCACGGCGACCCGTGCAACGTCTCCAGCGTGCCCTACATGATCGTCTTCGGCGTCGCCGAGGTCTTCTTCTCGCAGATCCCCGACTTCGATCAGATCTCCTGGCTCTCCATGCTCGCCGCCGTCATGTCCTTCACCTACTCCGTCATCGGCCTCAGCCTCGGCATCGTCCAAGTCGTCGCGAACGGAGGGTTGAAGGGAAGCCTGACCGGGATCAGCATCGGCGTGGTGACGCCGATGGACAAGGTGTGGAGGAGCCTGCAGGCGTTCGGCGACATCGCGTTCGCCTACTCCTACTCGCTGATCCTCATCGAGATCCAGGACACCATCCGGGCGCCGCCGCCGTCGGAGTCGGCGGTGATGAAGCGCGCCACGGTGGTGAGCGTGGCGGTGACCACGGTGTTCTACATGCTCTGCGGCAGCATGGGGTACGCGGCGTTCGGCGACGACGCGCCGGGGAACCTCCTCACCGGGTTCGGCTTCTACGAGCCCTTCTGGCTCCTCGACATCGCCAACGCCGCCATCGTCGTCCACCTCGTCGGCGCCTACCAGGTGTTCTGCCAGCCGCTCTTCGCCTTCGTCGAGAAGTGGGCGGCGCAGCGGTGGCCGGAGTCGCCGTACATCACCGGGGAGGTGGAGCTCCGCCTCTCGCCGTCGTCGAGGCGGTGCAGGGTGAACCTGTTCCGGTCGACGTGGCGCACGGCGTTCGTCGTCGCCACCACGGTGGTGTCCATGCTGCTGCCCTTCTT

CTTCCCCGTGGAGATGTACGTGGTGCAGAAGAAGGTGCCACGGTGGAGCACACGGTGGGTGTGCCTGCAGATGCTCAGCGTCGGCTGCCTCGTCATCTCCATCGCCGCCGCCGCGGGCTCCATCGCCGGCGTCATGTCGGATCTCAAGGTTTACCGCCCGTT CAAGGGTTACTGASOYBEAN SEQ ID NO: 31: KHN37208 AAP6 [Glycine soja] (protein)MFVETPEDGGKNFDDDGRVKRTGTWITASAHIITAVIGSGVLSLAWAIAQMGWVAGPAVLFAFSFITYFTSTLLADCYRSPDPVHGKRNYTYSDVVRSVLGGRKFQLCGLAQYINLVGVTIGYTITASISMVAVKRSNCFHKHGHHDKCYTSNNPFMILFACIQIVLSQIPNFHKLWWLSIVAAVMSFAYSSIGLGLSVAKVAGGGEPVRTTLTGVQVGVDVTGSEKVWRTFQAIGDIAFAYAYSNVLIEIQDTLKSSPPENKVMKRASLIGILTTTLFYVLCGCLGYAAFGNDAPGNFLTGFGFYEPFWLIDFANICIAVHLVGAYQVFCQPIFGFVENWGKERWPNSHF

FPIEMYIKQSKMQKFSFTWTWLKILSWACLIVSIISAAGSIQGLAQDLKKYQPFKAQQSEQ ID NO: 32: KHN37208 AAP6 [Glycine soja] (genomic)ATGTTCGTAGAAACCCCTGAAGATGGTGGCAAAAACTTCGACGATGATGGACGAGTCAAAAGAACTGGTACATGGATAACTGCGAGTGCCCATATCATAACGGCAGTGATAGGTTCTGGAGTGTTGTCACTTGCATGGGCAATTGCACAAATGGGTTGGGTGGCAGGCCCTGCGGTTCTCTTTGCCTTCTCTTTCATCACATACTTCACCTCCACTCTTCTTGCCGACTGTTATCGTTCACCTGACCCTGTTCATGGCAAGCGAAACTACACCTATTCAGATGTTGTCAGATCCGTGTTAGGAGGTAGGAAATTTCAGCTGTGTGGATTAGCTCAGTACATAAATCTTGTCGGTGTAACTATCGGTTACACGATAACGGCTTCAATTAGTATGGTGGCGGTGAAGAGGTCCAACTGTTTTCACAAACATGGTCATCATGATAAGTGCTACACGTCAAACAACCCTTTCATGATCCTCTTTGCCTGCATTCAAATCGTGCTTAGTCAAATACCAAATTTCCATAAGCTTTGGTGGCTCTCCATTGTTGCAGCAGTTATGTCTTTTGCTTATTCTTCCATTGGCCTTGGGCTCTCCGTAGCTAAAGTGGCAGGTGGTGGAGAACCTGTACGGACAACCTTAACGGGGGTGCAAGTTGGGGTGGACGTTACGGGATCCGAGAAGGTCTGGAGGACGTTTCAAGCTATTGGTGATATTGCCTTCGCTTACGCTTATTCTAACGTGCTCATTGAGATACAGGATACCCTGAAATCGAGCCCTCCAGAAAACAAGGTCATGAAAAGAGCAAGTTTGATTGGCATCTTGACTACAACCTTGTTCTATGTGCTATGTGGCTGCCTAGGTTATGCAGCATTTGGAAACGACGCACCAGGAAATTTCCTCACAGGGTTCGGTTTCTACGAGCCCTTTTGGCTAATAGACTTTGCTAACATCTGCATAGCCGTACACTTGGTTGGAGCATATCAGGTCTTCTGTCAGCCCATATTTGGGTTCGTAGAGAACTGGGGTAAGGAAAGGTGGCCCAATAGCCATTTTGTAAATGGAGAACACGCTTTAAAGTTTCCACTATTTGGAACCTTCCCTGTGAACTTTTTCAGGGTGGTATGGAGAACAACATATGTCATCATCACTGCTTTGATAGCTATGATGTT

GGTTTACTTCCCCATAGAGATGTACATTAAGCAGTCAAAGATGCAAAAGTTTTCCTTCACTTGGACATGGCTCAAGATATTGAGCTGGGCTTGCTTGATCGTTTCTATTATCTCAGCTGCTGGCTCCATCCAAGGCCTCGCTCAAGATCTCAAGAAATATCAGCCCTTCAAAGCCCAGCAATAASEQ ID NO: 33: XP_003526513 AAP6 [Glycine max] (protein)MNPDQFQKNSMFVETPEDGGKNFDDDGRVKRTGTWITASAHIITAVIGSGVLSLAWAIAQMGWVAGPAVLFAFSSFITYFTSTLLADCYRSPDPVHGKRNYTYSDVVRSVLGGRKFQLCGLAQYINLVGVTIGYTITASISMVAVKRSNCFHKHGHHDKCYTSNNPFMILFACIQIVLSQIPNFHKLWWLSIVAAVMSFAYSSIGLGLSVAKVAGGGEPVRTTLTGVQVGVDVTGSEKVWRTFQAIGDIAFAYAYSNVLIEIQDTLKSSPPENKVMKRASLIGILTTTLFYVLCGCLGYAAFGNDAPGNFLTGFGFYEPFWLIDFANICIAVHLVGAYQVFCQPIFGFVENWGKERWPNSHFVNGEHALKFPLFGTFPVNFFRVVWRTTYVIITALIAMMFPFFNDFLGLI

KYQPFKAQQ SEQ ID NO: 34: XP_003526513 AAP6 [Glycine max] (genomic)ATGAATCCTGATCAGTTTCAGAAGAACAGCATGTTCGTAGAAACCCCTGAAGATGGTGGCAAAAACTTCGACGATGATGGACGAGTCAAAAGAACTGGTACATGGATAACTGCGAGTGCCCATATCATAACGGCAGTGATAGGTTCTGGAGTGTTGTCACTTGCATGGGCAATTGCACAAATGGGTTGGGTGGCAGGCCCTGCGGTTCTCTTTGCCTTCTCTTTCATCACATACTTCACCTCCACTCTTCTTGCCGACTGTTATCGTTCACCTGACCCTGTTCATGGCAAGCGAAACTACACCTATTCAGATGTTGTCAGATCCGTGTTAGGAGGTAGGAAATTTCAGCTGTGTGGATTAGCTCAGTACATAAATCTTGTCGGTGTAACTATCGGTTACACGATAACGGCTTCAATTAGTATGGTGGCGGTGAAGAGGTCCAACTGTTTTCACAAACATGGTCATCATGATAAGTGCTACACGTCAAACAACCCTTTCATGATCCTCTTTGCCTGCATTCAAATCGTGCTTAGTCAAATACCAAATTTCCATAAGCTTTGGTGGCTCTCCATTGTTGCAGCAGTTATGTCTTTTGCTTATTCTTCCATTGGCCTTGGGCTCTCCGTAGCTAAAGTGGCAGGTGGTGGAGAACCTGTACGGACAACCTTAACGGGGGGTGCAAGTTGGGGTGGACGTTACGGGATCCGAGAAGGTCTGGAGGACGTTTCAAGCTATTGGTGATATTGCCTTCGCTTACGCTTATTCTAACGTGCTCATTGAGATACAGGATACCCTGAAATCGAGCCCTCCAGAAAACAAGGTCATGAAAAGAGCAAGTTTGATTGGCATCTTGACTACAACCTTGTTCTATGTGCTATGTGGCTGCCTAGGTTATGCAGCATTTGGAAACGACGCACCAGGAAATTTCCTCACAGGGTTCGGTTTCTACGAGCCCTTTTGGCTAATAGACTTTGCTAACATCTGCATAGCCGTACACTTGGTTGGAGCATATCAGGTCTTCTGTCAGCCCATATTTGGGTTCGTAGAGAACTGGGGTAAGGAAAGGTGGCCCAATAGCCATTTTGTAAATGGAGAACACGCTTTAAAGTTTCCACTATTTGGAACCTTCCCTGTGAACTTTTTCAGGGTGGTATGGAGAACAACATATGTCATCATCACTGCTTTGATAGCTATGATGTTTCCATTCTTCAATGACTTCCTAGG

ACATTAAGCAGTCAAAGATGCAAAAGTTTTCCTTCACTTGGACATGGCTCAAGATATTGAGCTGGGCTTGCTTGATCGTTTCTATTATCTCAGCTGCTGGCTCCATCCAAGGCCTCGCTCAAGATCTCAAGAAATATCAGCCCTTCAAAGCCCAGCAATAASEQ ID NO: 35: NP_001242816 LOC100777963 [Glycine max] (protein)MNSDQFQKNSMFVETPEDGGKNFDDDGRVRRTGTWITASAHIITAVIGSGVLSLAWAIAQMGWVAGPAVLFAFSFITYFTSTLLADCYRSPDPVHGKRNYTYSDVVRSVLGGRKFQLCGLAQYINLVGVTIGYTITASISMVAVKRSNCFHKHGHHVKCYTSNNPFMILFACIQIVLSQIPNFHKLWWLSIVAAVMSFAYSSIGLGLSVAKVAGGGEPVRTTLTGVQVGVDVTGSEKVWRTFQAIGDIAFAYAYSNVLIEIQDTLKSSPPENKVMKRASLIGILTTTLFYVLCGCLGYAAFGNDAPGNFLTGFGFYEPFWLIDFANICIAVHLVGAYQVFCQPIFGFVENWGRERWPNSQFVNGEHALNFPLCGTFPVNFFRVVWRTTYVIITALIAMMFPFFNDFLGLI

KYQPFKAQQSEQ ID NO: 36: NP_001242816 LOC100777963 [Glycine max] (genomic)ATGAATTCTGATCAGTTTCAGAAGAACAGCATGTTCGTAGAAACCCCTGAAGATGGTGGCAAAAACTTCGACGATGATGGACGAGTCAGAAGAACGGGTACATGGATAACTGCGAGTGCCCATATCATAACGGCAGTGATAGGGTCAGGAGTGTTGTCACTTGCATGGGCAATTGCACAAATGGGTTGGGTGGCTGGCCCTGCCGTTCTCTTTGCCTTCTCTTTCATCACTTACTTCCTTCCACTCTTCTTGCCGACTGTTATCGTTCACCTGATCCTGTTCATGGCAAGCGAAACTACACCTATTCCGATGTTGTCAGATCCGTCTTAGGAGGGAGGAAATTTCAGCTGTGTGGATTAGCTCAGTACATAAATCTTGTCGGTGTAACTATCGGTTACACGATAACGGCTTCAATTAGTATGGTGGCGGTGAAGAGGTCGAATTGTTTTCACAAACATGGTCATCATGTTAAGTGCTATACGTCAAACAACCCTTTCATGATCCTCTTTGCCTGCATTCAAATCGTGCTTAGCCAGATACCAAATTTCCATAAGCTCTGGTGGCTCTCCATTGTTGCAGCAGTTATGTCTTTTGCTTATTCTTCCATTGGCCTCGGGCTCTCAGTAGCTAAAGTGGCAGGTGGTGGAGAGCCTGTACGGACAACCTTAACGGGGGGTGCAAGTTGGGGTAGACGTTACAGGATCCGAGAAGGTCTGGAGGACGTTTCAAGCTATTGGTGACATTGCCTTCGCTTATGCTTATTCTAACGTGCTCATCGAGATACAGGATACCCTGAAATCGAGCCCTCCAGAGAACAAGGTCATGAAAAGAGCAAGTTTGATTGGCATCTTGACTACAACCTTGTTCTATGTGCTATGTGGCTGCCTAGGTTATGCAGCATTTGGAAACGATGCACCAGGAAATTTCCTTACAGGGTTCGGCTTCTACGAGCCCTTTTGGCTCATAGACTTTGCCTAACATCTGCATAGCCGTGCACTTGGTTGGAGCATATCAGGTCTTCTGTCAGCCCATATTTGGGTTCGTAGAGAACTGGGGTAGGGAAAGGTGGCCAAATAGCCAATTTGTAAATGGAGAACACGCTTTGAACTTTCCACTATGTGGAACCTTCCCTGTGAACTTCTTCAGGGTGGTGTGGAGAACAACATATGTCATCATCACTGCTTTGATAGCTATGATGTTTCCATTCTTCAATGACTTCCTAGG

ACATTAAGCAGTCAAAGATGCAAAGGTTTTCCTTCACGTGGACGTGGCTCAAGATCTGAGCTGGGCTTGCTTGATCGTTTCTATTATCTCAGCTGCTGGTTCCATCCAAGGCCTCGCTCAAGATCTCAAGAAATATCAGCCCTTCAAAGCCCAGCAATAASEQ ID NO: 37: XP_028228300 AAP6-like [Glycine soja] (protein)MNSDQFQKNSMFVETPEDGGKNFDDDGRVRRTGTWWITASAHIITAVIGSGVLSLAWAIAQMGWVAGPAVLFAFSFITYFTSTLLADCYRSPDPVHGKRNYTYSDVVRSVLGGRKFQLCGLAQYINLVGVTIGYTITASISMVAVKRSSNCFHKHGHHVKCYTSNNPFMILFACIQIVLSQIPNFHKLWWLSIVAAVMSFAYSSIGLGLSVAKVAGGGEPVRTTLTGVQVGVDVTGSEKVWRTFQAIGDIAFAYAYSNVLIEIQDTLKSSPPENKVMKRASLIGILTTTLFYVLCGCLGYAAFGNDAPGNFLTGFGFYEPFWLIDFANICIAVHLVGAYQVFCQPIFGFVENWGKERWPNSQFVNGEHALNFPLCGTFPVNFFRVVWRTTYVIITALIAMMFPFFNDFLGLI

KYQPFKAQQ SEQ ID NO: 38: XP_028228300 AAP6-like [Glycine soja] (genomic)ATGAATTCTGATCAGTTTCAGAAGAACAGCATGTTCGTAGAAACCCCTGAAGATGGTGGCAAAAACTTCGACGATGATGGACGAGTCAGAAGAACTGGTACATGGATAACTGCGAGTGCCCATATCATAACGGCAGTGATAGGGTCAGGAGTGTTGTCACTTGCATGGGCAATTGCACAAATGGGTTGGGTGGCTGGCCCTGCCGTTCTCTTTGCCTTCTCTTTCATCACTTACTTCACTTCCACTCTTCTTGCCGACTGTTATCGTTCACCTGATCCTGTTCATGGCAAGCGAAACTACACCTATTCCGATGTTGTCAGATCCGTCTTAGGAGGGAGGAAATTTCAGCTGTGTGGATTAGCTCAGTACATAAATCTTGTCGGTGTAACTATCGGTTACACGATAACGGCTTCAATTAGTATGGTGGCGGTGAAGAGGTCGAATTGTTTTCACAAACATGGTCATCATGTTAAGTGCTATACGTCAAACAACCCTTTCATGATCCTCTTTGCCTGCATTCAAATCGTGCTTAGCCAGATACCAAATTTCCATAAGCTCTGGTGGCTCTCCATTGTTGCAGCAGTTATGTCTTTTGCTTATTCTTCCATTGGCCTCGGGCTCTCAGTAGCTAAAGTGGCAGGTGGTGGAGAGCCTGTACGGACAACCTTAACGGGGGTGCAAGTTGGGGTAGACGTTACAGGATCCGAGAAGGTCTGGAGGACGTTTCAAGCTATTGGTGACATTGCCTTCGCTTATGCTTATTCTAACGTGCTCATCGAGATACAGGATACCCTGAAATCGAGCCCTCCAGAGAACAAGGTCATGAAAAGAGCAAGTTTGATTGGCATCTTGACTACAACCTTGTTCTATGTGCTATGTGGCTGCCTAGGTTATGCAGCATTTGGAAACGATGCACCAGGAAATTTCCTCACAGGGTTCGGCTTCTACGAGCCCTTTTGGCTCATAGACTTTGCTAACATCTGCATAGCCGTGCACTTGGTTGGAGCATATCAGGTCTTCTGTCAGCCCATATTTGGGTTCGTAGAGAACTGGGGTAAGGAAAGGTGGCCCAATAGCCAATTTGTAAATGGAGAACACGCTTTGAACTTTCCACTATGTGGAACCTTCCCTGTGAACTTCTTCAGGGTGGTGTGGAGAACAACATATGTCATCATCACTGCTTTGATAGCTATGATGTTTCCATTCTTCAATGACTTCCTAGG

ACATTAAGCAGTCAAAGATGCAAAGGTTTTCCTTCACGTGGACATGGCTCAAGATACTGAGCTGGGCTTGCTTGATCGTTTITCTATTATCTCAGCTGCTGGTTCCATCCAAGGCCTCGCTCAAGATCTCAAGAAATATCAACCCTTCAAAGCCCAGCAATAASEQ ID NO: 39: KRH353636.1 hypothetical protein GLYMA_10G255300 [Glycine max](protein) MAVIRSNCFHKYGHEAKCHTSNYPYMTIFAVIQILLSSQIPDFQELSGLSIIAAVMSFGYSSIGIGLSIAKIAGGNDAKTSLTGLIVGEDVTSQEKLWNTFQAIGNIAFAYAFSQVLVEIQDTLKSSSPPENQAMKKATLAGCSITSLFYMLCGLLGYAAFGNKAPGNFLTGFGFYEPYWLVDIGNVFVFVHLVGAYQVFTQPVFQLVETWVAKRWPESNFMGKEYRVGKFRFNGF

GVKILSGFCLIVTLVAAAGSIQGIIADLKIYEPFKSEQ ID NO: 40: KRH353636.1 hypothetical protein GLYMA_10G255300 [Glycine max](genomic) ATGGCTGTCATAAGATCGAATTGCTTTCACAAGTATGGGCACGAAGCGAAGTGTCATACATCAAATTACCCATATATGACCATCTTTGCGGTCATACAGATTTTATTAAGCCAAATCCCTGATTTCCAGGAACTCTCAGGCCTCTCTATTATTGCTGCCGTCATGTCTTTTGGTTATTCTTCCATAGGCATTGGTCTCTCCATAGCCAAAATTGCAGGAGGAAACGATGCCAAGACAAGTCTAACGGGGCTCATCGTTGGAGAAGACGTGACAAGCCAGGAGAAACTATGGAACACTTTCCAAGCAATTGGAAACATTGCTTTTGCATACGCCTTCAGTCAAGTACTTGTTGAGATACAGGACACGTTAAAATCAAGCCCACCAGAAAATCAAGCCATGAAAAAGGCAACCCTTGCTGGATGCTCGATCACCTCACTGTTTTATATGTTATGTGGCCTATTAGGCTATGCAGCATTCGGGAACAAGGCACCCGGAAACTTCTTAACAGGATTTGGGTTTTATGAACCATATTGGCTTGTTGACATTGGTAATGTCTTCGTATTTGTTCATTTAGTGGGCGCCTACCAGGTATTCACACAACCAGTTTTCCAGCTTGTGGAAACTTGGGTTGCGAAGCGTTGGCCTGAAAGCAACTTCATGGGAAAAGAATATCGTGTTGGCAAGTTCAGATTCAATGGATTCAGGATCATATGGAGGACAGTGTACGTGATTTTCACAGCAGTGGTTGCTATGATACTTCCCTTCTTCAACAGCATTGTGG

ATCTGGTGCAGGCTAAAGTGCCCAAGTTTTCTCTGGTCTGGATTGGGGTCAAATTCTAAGTGGCTTCTGCTTGATTGTCACTCTTGTTGCTGCAGCTGGATCAATCCAAGGAATCATCGCAGACCTTAAAATCTATGAGCCCTTCAAGTAASEQ ID NO: 41: XP_028192809.1 AAP4-like [Glycine soja] (protein)MLPRSRTLPSRIHQGIIEERHDVRPYVQVEVRPNNIQTETQAMNIQSNYSKCFDDDGRLKRTGTFWTATAHIITAVIGSGVLSLAWAVAQLGWWVAGPVVMFLFAVVNLYTSNLLTQCYRTGDSVNGHRNYTYMEAVKSILGGKKVKLCGLIQYINLFGVAIGYTIAASVSMMAIKRSNCYHSSHGKDPCHMSSNGYMITFGIAEVIFSQIPDFDQVWWLSIVAAIMSFTYSSVGLSLGVAKVAENKTFKGSLMGISIGTVTQAGTVTSTQKIWRSLQALGAMAFAYSFSIILIEIQDTIKSPPAEHKTMRKATTLSIAVTTVFYLLCGCMGYAAFGDNAPGNLLTGFGFYNPYWLLDIANLAIVIHLVGAYQVFSQPLFAFVEKWWSVRKWPKSNFVTAEYDIPIPCFGVYQ

SRWIGLQLLSVSCLIISLLAAVGSMGVVLDLKTYKPFKTSYSEQ ID NO: 42: XP_028192809.1 AAP4-like [Glycine soja] (genomic)ATGTTGCCAAGAAGTAGAACCCTTCCTAGCAGAATCCACCAAGGAATTATAGAAGAGAGGCACGATGTCAGGCCCTACGTACAAGTAGAAGTGCGACCCAATAATATCCAAACGGAGACCCAAGCGATGAATATCCAGTCTAACTATTCCAAGTGCTTCGATGATGATGGTCGCTTGAAGAGAACAGGAACATTTTGGACGGCAACTGCTCATATCATCACTGCTGTGATAGGGTCGGGAGTCCTTTCACTAGCATGGGCGGTTGCTCAGCTTGGTTGGGTTGCTGGACCTGTTGTCATGTTTCTCTTTGCCGTCGTCAATCTCTACACTTCCAACCTATTAACACAGTGTTACAGGACCGGTGACTCCGTTAATGGGCACAGAAATTACACCTACATGGAGGCTGTCAAGTCCATCTTGGGAGGAAAAAAGGTCAAGTTATGTGGCCTCATCCAATATATCAATCTGTTTGGAGTTGCAATCGGGTACACCATTGCTGCCTCTGTCAGTATGATGGCCATAAAAAGGTCGAATTGCTATCACAGCAGTCATGGAAAGATCCCTGCCACATGTCAAGCAATGGGTATATGATAACATTTGGAATAGCAGAAGTGATATTTTCCCAAATCCCAGACTTTGATCAGGTGTGGTGGCTATCCATAGTTGCAGCTATCATGTCCTTCACTTATTCTTCAGTTGGATTGAGTCTTGGAGTGGCCAAAGTAGCAGAAAATAAAACTTTCAAAGGAAGCCTGATGGGAATTAGCATTGGCACAGTAACACAAGCCGGAACAGTCACCAGCACACAGAAAATATGGAGGAGTTTACAAGCTCTTGGGGCAATGGCCTTTGCATACTCCTTTTCCATTATCCTCATCGAAATTCAGGACACCATAAAATCTCCTCCTGCAGAGCACAAGACCATGAGAAAGGCCACAACATTGAGCATCGCGGTTACCACAGTGTTCTATTTACTCTGTGGATGCATGGGTTATGCAGCCTTCGGAGATAATGCACCTGGAAATCTCTTGACTGGTTTTGGGTTCTATAACCCTTATTGGCTTCTGGACATTGCCAACCTTGCAATTGTTATCCACCTAGTTGGGGCATATCAGGTTTTTCCCAGCCCTTATTTGCATTTGTGGAAAAATGGAGTGTACGCAAATGGCCAAAGAGCAATTTTGTCACGGCAGAATATGATATACCGATTCCCTGCTTTGGTGTGTACCAACTCAACTTCTTCCGCTTAGTATGGAGAACCATTTTTGTGCTGTTGACGACCCTCATAGCCATGCTCATGCCTTTTTCAACGATGTGGTTGGAATACTTGGC

AAGAAGATTGGACGATGGACTAGTCGGTGGATTGGACTTCAATTACTTAGTGTCAGTTGCCTCATCATTTCATTGTTAGCTGCAGTTGGTTCCATGGCAGGGGTTGTTTTGGACCTCAAGACTTATAAGCCATTTAAAACTAGTTATTAASEQ ID NO: 43: XP_00659084.1; XP_003540867; AAP4 [Glycine max] (protein)MLPRSRTLPSRIHQGIIEERHNVRHYLQVEVRPNNTQTETEAMNIQSNYSKCFDDDGRLKRTGTFWMATAHIITAVIGSGVLSLAWAVAQLGWVAGPIVMFLFAVVNLYTSNLLTQCYRTGDSVTGHRNYTYMEAVNSILGGKKVKLCGLIQYINLFGVAIGYTIAASVSMMAIKRSNCYHSSHGKDPCHMSSNGYMITFGIAEVIFSQIPDFDQVWWLSIVAAIMSFTYSSVGLSLGVAKVAENKSFKGSLMGISIGTVTQAGTVTSTQKIWRSLQALGAMAFAYSFSIILIEIQDTIKSPPAEHKTMRKATTLSIAVTTVFYLLCGCMGYAAFGDNAPGNLLTGFGFYNPYWLLDIANLAIVIHLVGAYQVFSQPLFAFVEKWWSARKWPKSNFVTAEYDIPIPCFGVYQL

RWWLGLQLLSASCLIISLLAAVGSMAGVVLDLKTYKPFKTSYSEQ ID NO: 44: XP_006590854.1; XP_003540867; AAP4 [Glycine max] (genomic)ATGTTGCCAAGAAGTAGAACCCTTCCTAGCAGAATCCACCAAGGAATTATAGAAGAGAGGCATAATGTCAGGCACTACTTACAAGTTGAAGTGCGACCCAATAATACCCAAACGGAGACCGAAGCGATGAATATCCAGTCTAACTATTCCAAGTGCTTCGATGATGATGGTCGCTTGAAGAGAACAGGAACATTTTGGATGGCAACTGCTCATATCATCACTGCTGTGATAGGCTCAGGAGTCCTTTCACTAGCATGGGCGGTTGCTCAGCTTGGTTGGGTTGCTGGACCTATTGTCATGTTTCTCTTTGCCGTCGTCAATCTCTACACTTCCAACCTATTAACACAGTGTTACAGGACCGGTGACTCCGTTACTGGACACAGAAATTACACCTACATGGAGGCAGTCAACTCCATCTTGGGAGGAAAAAAGGTCAAGTTATGTGGCCTCATCCAATATATCAATCTGTTTGGAGTTGCAATTGGATACACCATTGCTGCCTCTGTCAGTATGATGGCCATAAAAAGGTCGAATTGTTATCACAGCAGTCATGGAAAAGATCCCTGCCACATGTCAAGCAATGGGTATATGATAACATTCGGAATAGCAGAAGTGATATTTTCCCAAATCCCAGACTTTGATCAGGTGTGGTGGCTATCCATAGTTGCAGCTATCATGTCCTTCACTTATTCTTCAGTTGGATTGAGTCTTGGCGTGGCCAAAGTAGCAGAAAATAAAAGTTTCAAAGGAAGCCTGATGGGAATTAGCATTGGCACAGTAACACAAGCCGGAACAGTCACTAGCACACAGAAAATATGGAGGAGTTTACAAGCTCTCGGGGCAATGGCCTTTGCATACTCCTTTTCCATTATCCTCATCGAAATTCAGGACACCATAAAATCTCCTCCTGCAGAGCACAAGACCATGAGAAAGGCCACAACTTTGAGCATCGCAGTTACTACAGTGTTCTATTTACTCTGTGGATGCATGGGTTATGCAGCCTTCGGAGATAATGCACCTGGAAACCTCTTGACTGGTTTTGGGTTCTATAACCCTTACTGGCTTCTGGACATTGCCAACCTTGCAATTGTTATCCACCTAGTTGGGGCATACCAGGTTTTTTCCCAGCCCTTATTTGCATTTGTGGAAAAATGGAGTGCACGTAAATGGCCAAAGAGCAATTTTGTCACCGCAGAATATGATATACCCATTCCCTGCTTTGGTGTGTACCAACTCAACTTCTTCCGCTTAGTATGGAGGACCATTTTTGTGCTGTTGACGACCCTCATAGCCATGCTCATGCCTTTTTTCAACGATGTGGTTGGAATACTTGGCGCT

AAGATTGGACGATGGACCAGTCGCTGGCTTGGACTTCAGTTACTTAGTGCCAGTTGCCTCATCATTTCATTGTTAGCTGCAGTTGGTTCCATGGCAGGGGTGGTTTTGGACCTCAAGACTTACAAGCCATTTAAAACTAGTTATTAASEQ ID NO: 45: RZB79331.1 AAP2 isoform B [Glycine soja] (protein)MNIQSNYSKCFDDDGRLKRTGTFWMATAHIITAVIGSGVLSLAWAVAQLGWVAGPIVMFLFAVVNLYTSNLLTQCYRTGDSVTGHRNYTYMEAVNSILGGKKVKLCGLIQYINLFGVAIGYTIAASVSMMAIKRSNCYHSSHGKDPCHMSSNGYMITFGIAEVIFSQIPDFDQVWWLSIVAAIMSFTYSSVGLSLGVAKAENKSFKGSLMGISIGTVTQAGTVTSTQKIWRSLQALGAMAFAYSFSIILIEIQDTIKSPPAEHKTMRKATTLSIAVTTVFYLLCGCMGYAAFGDNAPGNLLTGFGFYNPYWLLDIANLAIVIHLVGAYQVFSQPLFAFVEKWSARKWPKS

VYFPIDMYISQKKIGRWTSRWLGLQLLSASCLIISLLAAVGSMAGVVLDLKTYKPFKTSYSEQ ID NO: 46: RZB79331.1 AAP2 isoform B [Glycine soja] (genomic)ATGAATATCCAGTCTAACTATTCCAAGTGCTTCGATGATGATGGTCGCTTGAAGAGAACAGGAACATTTTGGATGGCAACTGCTCATATCATCACTGCTGTGATAGGCTCAGGAGTCCTTTCACTAGCATGGGCGGTTGCTCAGCTTGGTTGGGTTGCTGGACCTATTGTCATGTTTCTCTTTGCCGTCGTCAATCTCTACACTTCCAACCTATTAACACAGTGTTACAGGACCGGTGACTCCGTTACTGGACACAGAAATTACACCTACATGGAGGCAGTCAACTCCATCTTGGGAGGAAAAAAGGTCAAGTTATGTGGCCTCATCCAATATATCAATCTGTTTGGAGTTGCAATTGGATACACCATTGCTGCCTCTGTCAGTATGATGGCCATAAAAAGGTCGAATTGTTATCACAGCAGTCATGGAAAAGATCCCTGCCACATGTCAAGCAATGGGTATATGATAACATTCGGAATAGCAGAAGTGATATTTTCCCAAATCCCAGACTTTGATCAGGTGTGGTGGCTATCCATAGTTGCAGCTATCATGTCCTTCACTTATTCTTCAGTTGGATTGAGTCTTGGCGTGGCCAAAGTAGCAGAAAATAAAAGTTTCAAAGGAAGCCTGATGGGAATTAGCATTGGCACAGTAACACAAGCCGGAACAGTCACTAGCACACAGAAAATATGGAGGAGTTTACAAGCTCTCGGGGCAATGGCCTTTGCATACTCCTTTTCCATTATCCTCATCGAAATTCAGGACACCATAAAATCTCCTCCTGCAGAGCACAAGACCATGAGAAAGGCCACAACTTTGAGCATCGCAGTTACTACAGTGTTCTATTTACTCTGTGGATGCATGGGTTATGCAGCCTTCGGAGATAATGCACCTGGAAACCTCTTGACTGGTTTTGGGTTCTATAACCCTTACTGGCTTCTGGACATTGCCAACCTTGCAATTGTTATCCACCTAGTTGGGGCATACCAGGTTTTTTCCCAGCCCTTATTTGCATTTGTGGAAAAATGGAGTGCACGTAAATGGCCAAAGAGCAATTTTGTCACCGCAGAATATGATATACCCATTCCCTGCTTTGGTGTGTACCAACTCAACTTCTTCCGCTTAGTATGGAGGACCATTTTTGTGCTGTTGACGACCCTCATAGCCATGCTC

GACAGTTTATTTCCCTATTGACATGTATATTTCGCAAAAGAAGATTGGACGATGGACCAGTCGCTGGCTTGGACTTCAGTTACTTAGTGCCAGTTGCCTCATCATTTCATTGTTAGCTGCAGTTGGTTCCATGGCAGGGGTGGTTTTGGACCTCAAGACTTACAAGCCATTTAAAACTAGTTATTAASEQ ID NO: 47: AAK33098.1 amino acid transporter [Glycine max] (protein)MLPRSRTLPSRIHQGIIEERHNVRHYLQVEVRPNNTQTETEAMNIQSNYSKCFDDDGRLKRTGTFWMATAHIITAVIGSGVLSLAWAVAQLGWVAGPIVMFLFAVVNLYTSNLLTQCYRTGDSVSGHRNYTYMEAVNSILGGKKVKLCGLTQYINLFGVAIGYTIAASVSMMAIKRSNCYHSSHGKDPCHMSSNGYMITFGIAEVIFSQIPDFDQVWWLSIVAAIMSFTYSSVGLSLGVAKVAENKSFKGSLMGISIGTVTQAGTVTSTQKIWRSLQALGAMAFAYSFSIILIEIQDTIKSPPAEHKTMRKATTLSIAVTTVFYLLCGCMGYAAFGDNAPGNLLTGFGFYNPYWLLDIANLAIVIHLVGAYQVFSQPLFAFVEKWSARKWPKSNFVTAEYDIPIPCFGVYQL

RWLGLQLLSASCLIISLLAAVGSMAGVVLDLKTYKPFKTSYSEQ ID NO: 48: AAK33098.1 amino acid transporter [Glycine max] (genomic)ATGTTGCCAAGAAGTAGAACCCTTCCTAGCAGAATCCACCAAGGAATTATAGAAGAGAGGCATAATGTCAGGCACTACTTACAAGTTGAAGTGCGACCCAATAATACCCAAACGGAGACCGAAGCGATGAATATCCAGTCTAACTATTCCAAGTGCTTCGATGATGATGGTCGCTTGAAGAGAACAGGAACATTTTGGATGGCAACTGCTCATATCATCACTGCTGTGATAGGCTCAGGAGTCCTTTCACTAGCATGGGCGGTTGCTCAGCTTGGTTGGGTTGCTGGACCTATTGTCATGTTTCTCTTTGCCGTCGTCAATCTCTACACTTCCAACCTATTAACACAGTGTTACAGGACCGGTGACTCCGTTTCTGGACACAGAAATTACACCTACATGGAGGCAGTCAACTCCATCTTGGGAGGAAAAAAGGTCAAGTTATGTGGCCTCACCCAATATATCAATCTGTTTGGAGTTGCAATTGGATACACCATTGCTGCCTCTGTCAGTATGATGGCCATAAAAAGGTCGAATTGTTATCACAGCAGTCATGGAAAAGATCCCTGCCACATGTCAAGCAATGGGTATATGATAACATTCGGAATAGCAGAAGTGATATTTTCCCAAATCCCAGACTTTGATCAGGTGTGGTGGCTATCCATAGTTGCAGCTATCATGTCCTTCACTTATTCTTCAGTTGGATTGAGTCTTGGCGTGGCCAAAGTAGCAGAAAATAAAAGTTTCAAAGGAAGCCTGATGGGAATTAGCATTGGCACAGTAACACAAGCCGGAACAGTCACTAGCACACAGAAAATATGGAGGAGTTTACAAGCTCTCGGGGCAATGGCCTTTGCATACTCCTTTTCCATTATCCTCATCGAAATTCAGGACCACCATAAAATCTCCTCCTGCAGAGCACAAGACCATGAGAAAGGCCACAACTTTGAGCATCGCAGTTACTACAGTGTTCTATTTACTCTGTGGATGCATGGGTTATGCAGCCTTCGGAGATAATGCACCTGGAAACCTCTTGACTGGTTTTGGGTTCTATAACCCTTACTGGCTTCTGGACATTGCCAACCTTGCAATTGTTATCCACCTAGTTGGGGCATACCAGGTTTTTTCCCAGCCCTTATTTGCATTTGTGGAAAAATGGAGTGCACGTAAATGGCCAAAGAGCAATTTTGTCACCGCAGAATATGATATACCCATTCCCTGCTTTGGTGTGTACCAACTCAACTTCTTCCGCTTAGTATGGAGGACCATTTTTGTGCTGTTGACGACCCTCATAGCCATGCTCATGCCTTTTTTCAACGATGTGGTTGGAATACTTGGCGCT

AAGATTGGACGATGGACCAGTCGCTGGCTTGGACTTCAGTTACTTAGTGCCAGTTGCCTCATCATTTCATTGTTAGCTGCAGTTGGTTCCATGGCAGGGGTGGTTTTGGACCTCAAGACTTACAAGCCATTTAAAACTAGTTATTAASEQ ID NO: 49: XP_003542145.1; XP_006596210; XP_003522571;XP_003527948; AAP3 [Glycine max] (protein)MMENGGKQTFEVSNDTLQRVGSKSFDDDGRLKRTGTIWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPVVMILFSIVTYYTSTLLATCYRSGDQLSGKRNYTYTQAVRSYLGGFSVKCGWVQYANLFGVAIGYTIAASISMMAIKRSNCYHSSGGKNPCKMNSNWYMISYGVSEIIFSQIPDFHELWWLSIVAAVMSFTYSFIGLGLGIGKVIGNGRIKGSLTGVTIGVTESQKIWRTFQALGNIAFAYSYSMILIEIQDTIKSPPAESETMSKATLISVLVTTVFYMLCGCFGYASFGDASPGNLLTGFGFYNPFWLIDIANAGIVIHLVGAYQVYCQPLFSFVESNAA

FWPLTVYLPVEMYITQTKIPKWGIKWIGLQMLSVACFVITILAAAGSIAGVIDDLKVYKPF VTSYSEQ ID NO: 50: XP_003542145.1; XP_006596210; XP_003522571;XP_003527948; APP3 [Glycine max] (genomic)ATGATGGAAAACGGTGGCAAACAGACATTTGAAGTCTCCAATGACACGCTTCAACGAGTAGGTTCCAAGAGCTTTGATGATGATGGCCGTCTCAAAAGAACTGGAACTATTTGGACTGCAAGTGCCCACATAATAACAGCTGTTATTGGTTCTGGGGTGCTATCTTTGGCTTGGGCTATTGCTCAGCTAGGTTGGATTGCTGGTCCTGTGGTGATGATTCTATTCTCTATTGTGACTTATTATACCTCAACTCTTCTAGCTACTTGTTACCGTTCTGGTGACCAACTCAGTGGCAAGAGAAACTACACTTACACACAAGCTGTTAGATCCTACCTTGGCGGTTTTTCGGTCAAGTTTTGTGGGTGGGTTCAGTATGCGAACCTTTTTGGAGTGGCAATTGGGTACACCATAGCAGCTTCCATAAGCATGATGGCAATCAAAAGGTCTAATTGTTATCATAGTAGCGGGGGGAAAAATCCATGCAAAATGAACAGCAATTGGTACATGATTTCATATGGTGTTTCGGAAATTATCTTCTCCCAAATTCCAGATTTCCATGAGTTGTGGTGGCTCTCTATTGTAGCTGCTGTCATGTCCTTCACATACTCATTCATTGGACTTGGCCTTGGTATTGGTAAAGTTATAGGAAACGGAAGAATTAAAGGAAGCCTAACTGGTGTAACTATTGGGACTGTGACAGAATCCCAAAAAATTTGGAGAACTTTCCAAGCGCTTGGAAACATAGCCTTTGCTTACTCCTACTCAATGATCCTTATTGAAATTCAGGACACAATCAAATCCCCTCCAGCAGAGTCAGAGACAATGTCCAAGGCTACTTTAATAAGTGTTTTGGTCACAACCGTTTTCTATATGCTATGTGGTTGCTTTGGCTATGCTTCTTTTGGAGATGCAAGTCCGGGAAACCTTCTCACTGGCTTTGGCTTCTATAACCCATTTTGGCTCATTGACATAGCCAATGCTGGCATTGTTATCCACCTTGTTGGTGCATACCAAGTTTACTGCCAACCCCTCTTCTCATTCGTCGAATCAAATGCGGCAGAAAGGTTCCCTAATAGTGATTTTATGAGCAGAGAGTTTGAAGTACCAATCCCTGGTTGCAAACCCTACAAGCTCAACCTCTTCAGGTTGGTTTGGAGGACACTTTTTGTGATTTTGTCAACTGTGATAGCCATGCTCCTACCATTCTTCAATGACATTGTAGGGCTTAT

CTCAAACTAAGATACCAAAGTGGGGCATAAAATGGATAGGCCTACAAATGCTTAGTGTTGCATGCTTTGTAATTACTATATTAGCTGCAGCAGGTTCCATTGCTGGGGTTATTGATGATCTTAAAGTTTACAAGCCATTTGTTACCAGCTACTAASEQ ID NO: 51: KHN19623.1; KHN44307; AAP 3 [Glycine soja] (protein)MENGGKQTFEVSNDTLQQGGSKSFDDDGRLKRTGTIWTASAHIVTAVIGSGVLSLAWAIAQLGWLAGPIVMILFSIVTYYTSTLLACCYRSGDQLSGKRNYTYTQAVRSNLGGLAVMFCGWVQYANLFGVAIGYTIAASISMMAVKRSNCYHSSGGKNPCKMNSNWYMISYGVAEIIFSQIPDFHELWWLSIVAAVMSFTYSFIGLGLGIGKVIGNGRIKGSLTGVTVGTVTESQKIWRSFQALGNIAFAYSYSMILIEIQDTIKSPPAESQTMSKATLISVLITTVFYMLCGCFGYASFGDASPGNLLTGFGFYNPYWLIDIANVGIVIHLVGAYQVYCQPLFSFVESHAAA

WPLTVYLPVEMYITQTKIPKWGPRWICLQMLSAACFVVTLLAAAGSIAGVIDDLKVYKP FVTSYSEQ ID NO: 52: KHN19623.1; KHN44307; AAP 3 [Glycine soja] (genomic)ATGGAAAACGGTGGCAAACAGACATTTGAAGTCTCAAATGACACGCTTCAACAAGGAGGTTCCAAGAGCTTTGATGATGATGGCCGTCTCAAAAGAACTGGAACTATATGGACTGCAAGTGCCCACATAGTAACAGCTGTTATTGGTTCTGGGGTGCTATCTTTGGCTTGGGCGATTGCTCAGCTAGGTTGGCTTGCTGGTCCTATTGTGATGATTCTGTTCTCTATTGTGACTTATTATACCTCAACTCTTCTAGCTTGTTGTTACCGTTCTGGTGACCAACTCAGTGGCAAGAGAAACTACACTTACACACAAGCTGTTAGATCCAACCTTGGTGGTCTTGCGGTCATGTTTTGTGGGTGGGTTCAGTATGCAAACCTATTTGGAGTGGCAATTGGGTACACCATAGCAGCTTCCATAAGCATGATGGCAGTCAAAAGGTCTAATTGTTATCATAGTAGCGGAGGGAAAAAATCCATGCAAAATGAATAGCAATTGGTACATGATTTCATATGGTGTTGCGGAAATTATCTTCTCCCAAATTCCAGATTTCCATGAGTTGTGGTGGCTCTCTATTGTAGCTGCTGTCATGTCCTTCACATACTCATTCATTGGACTTGGCCTTGGTATTGGTAAAGTTATAGGAAACGGAAGAATTAAAGGAAGCCTAACTGGTGTAACTGTTGGGACTGGTGACAGAATCCCAGAAAATTTGGAGGAGTTTCCAAGCTCTTGGTAACATAGCCTTTGCCTACTCCTACTCAATGATCCTTATTGAAATTCAGGACACAATCAAATCTCCTCCAGCAGAGTCACAGACAATGTCCAAGGCTACTTTAATCAGTGTTTTGATCACAACCGTTTTCTATATGTTATGTGGCTGCTTTGGCTATGCTTCTTTCGGAGATGCAAGCCCGGGAAACCTTCTCACTGGCTTCGGCTTCTATAACCCATATTGGCTCATTGACATAGCCAATGTTGGCATAGTTATCCACCTTGTTGGTGCATACCAAGTTTACTGCCAACCCCTCTTCTCATTCGTGGAATCACATGCAGCAGCAAGGTTCCCAAATAGTGATTTTATGAGCAGAGAGTTTGAAGTACCAATCCCTGGCTGCAAACCCTACAGGCTCAACCTCTTCAGGTTGGTTTGGAGGACAATTTTTGTGATTTTGTCAACTGTGATAGCCATGCTCCTACCATTCTTCAATGACATTGTAGGGCTTAT

CTCAAACTAAGATACCAAAGTGGGGCCCAAGATGGATATGCCTACAAATGCTTAGTGCTGCATGCTTTGTAGTTACTCTATTAGCTGCAGCAGGTTCCATTGCTGGGGTTATTGATGATCTTAAAGTTTACAAGCCATTCGTCACCAGCTACTAASEQ ID NO: 53: RZC18207.1 AAP3 isoform D [Glycine soja] (protein)MMCLRCTGTVWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMVLFSAITYYTSTLLSDCYRTGDPVTGKRNYTYMDAIQSNFGGNGFKVKLCGLVQYINLFGVAIGYTIAASTSMMAIERSNCYHKSGGKDPCHMNSNMYMISFGIVEIIFSQIPGFDQLWWLSIVAAVMSFTYSTIGLGLGIGKVIENRGVGGSLTGITIGTVTQTEKVWRTMQALGDIAFAYSYSLILVEIQDTVKSPPSESKTMKKASFISVAVTSIFYMLCGCFGYAAFGDASPGNLLTGFGFYNPYWLLDIANAAIVIHLVGSYQVYCQPLFAFVEKHAARMLPDSDFVNKEIEIPIPGFHSYKVNLF

WICLQILSMACLLMTIGAAAGSIAGIAIDLQTYKPFKTNYSEQ ID NO: 54: RZC18207.1 APP3 isoform D [Glycine soja] (genomic)ATGATGTGTTTGAGATGTACAGGGACGGTGTGGACTGCAAGTGCACACATAATACTGCAGTGATTGGGTCTGGGGTGCTGTCTCTGGCTTGGGCTATAGCTCAGCTTGGATGGATTGCTGGTCCTATTGTCATGGTTCTCTTTTCTGCCATCACTTACTACACTTCCACTCTTCTCTCTGATTGTTATCGTACTGGTGATCCTGTAACTGGCAAGAGAACTACACTTACATGGACGCTATTCAGTCTAACTTTGGTGGAAATGGCTTTAAGGTCAAGCTGTGTGGGCTAGTTCAGTACATTAACCTTTTCGGAGTCGCCATTGGTTACACTATAGCGGCTTCCACTAGCATGATGGCAATTGAAAGATCTAATTGTTACCACAAGAGTGGAGGGAAAGATCCATGTCATATGAACAGTAACATGTACATGATTTCATTTGGTATAGTGGAAATTATTTTCTCACAAATTCCGGGCTTCGATCAATTGTGGTGGCTCTCCATTGTAGCTGCTGTCATGTCCTTCACATACTCCACTATTGGGCTAGGCCTTGGTATTGGAAAAGTTATTGAAAATAGAGGAGTCGGGGGAAGCCTAACCGGGATAACAATTGGTACCGTGACACAAACTGAAAAAGTTTGGAGAACCATGCAAGCTCTTGGTGACATAGCCTTTGCCTATTCATACTCCCTCATCCTTGTAGAAATTCAGGACACAGTGAAATCCCCTCCATCAGAGTCAAAAACAATGAAGAAGGCTAGTTTCATCAGTGTTGCAGTAACCAGCATTTTCTACATGCTTTGTGGTTGCTTTGGTTATGCTGCTTTTGGAGATGCAAGCCCTGGAAACCTTCTCACTGGCTTTGGTTTCTACAACCCATATTGGCTCCTTGACATAGCTAATGCTGCCATAGTGATCCACCTTGTTGGTTCATACCAAGTTTACTGCCAGCCCCTCTTCGCCTTCGTTGAGAAACACGCGGCGCGTATGCTCCCAGATAGTGATTTTGTGAACAAAGAAATTGAAATTCCAATCCCTGGTTTCCATTCCTACAAGGTCAACCTTCTTCAGATTGGTTTGGAGGACAATATATGTAATGGTGAGCACTGTAATATCAA

CCCCTTACTGTGTATTTCCCAGTGGAGATGTACATTAATCAAAAGAGAATACCAAAATGGAGCACAAAGTGGATCTGCCTCCAAATACTTAGCATGGCTTGCCTTTTGATGACTATAGGAGCTGCAGCTGGCTCTATTGCTGGGATTGCCATTGATCTTCAAACTTACAAGCCTTTCAAAACCAACTATTGASEQ ID NO: 55: RZC13226.1 Amino acid permease 2 isoform B, partial [Glycine soja] (protein)KFALFLRVFCVWKFSFHQIKMPENAATTNLNHLQVFGIEDDVPSHSQNNSKCYDDDGRLKRTGNVWTASSHIITAVIGSGVLSLAWAIAQLGWIAGPTVMFLFSLVTFYTSSLLADCYRAGDPNSGKRNYTYMDAVRSILGGANVTLCGIFQYLNLLGIVGYTIAASISMMAIKRSNCFHKSGGKNPCHMSSNVYMIIFGATEIFLSQIPDFDQLWWLSTVAAIMSFTYSIIGLSLGIAKVAETGTFKGGLTGISIGPVSETQKIWRTSQALGDIAFAYSYAVVLIEIQDTIKSPPSEAKTMKKATLISIAVTTTFYMLCGCMGYAAFGDAAPGNLLTGFGFYNPYWLIDIANAAIVIHLVGAYQVFSQPIFAFVEKEVTQRWPHIEREFKIPIPGFSPYKLKVFRLVLRTVFVVL

SVVAAVGSVAGVLLDLKKYKPFHSHYSEQ ID NO: 56: RZC13226.1 Amino acid permease 2 isoform B, partial [Glycine soja](genomic): >Glyma.05G194600 | Chr05:37909533..37914347 reverse

ATACACGACTCTGTTTCATATAAACTAGAATTTAGTTAGAATGAATCGACACGCTGATAAACAATTTTTTATTATGTTATCTAATTATAAAAATTTTGTACAATAAAATTATTAAATTTTATAATAATAAAATTTATATTTGGTATATTTATTGACCGTGTAAACTGGTTTATAGAGATTTTATAACAACTCTTAAGTTTTAGTCTATTGACTTTAATAATAATTATCTTAAAATTTATATTTAACTTTATTTTTAAATAATTTTTTAATATTATCAATGTATCCATAAAAATGAAACTATAAAATTTAACTTTGTAATATAACTGTTCCACTATTTGAAAATCAAACATCAGACATTGTTAATTATTGTTTCTATCTCCATTTTTTTTTAAAATCTTTTTGCCCCACACACATCTCAAAATTCCTTCAAAAATCCAAGGAAATAACAAAAGCTGACCCTTTTTTTATGTACAAAAAGTATTTTTCATAATGTAAATTAATATATAATGTACATGTGATGATATG

TTTTTATATTCTTTTCACAAATTAATGATATGCATGCATGTTGTTGACCTTAAATGATACTATATCATATAGTACATATAGTATATATTGTTGGAAAGTCTCACATCATATTATCTATTTTAATTTTTGAGATGTAATTTATATATTTGTTGAGTAATTTTATAATATCAATAAGATGTATGTAATAAATTAAACATTAATAACTTATAATTAATGGGATTGTCATGATCATACTGATGATAAGGTCATTATCACCTCATGAAAATATATGAGATTATGTCCGCCAAAGAAGATGCTACTTGTCAAAAGGGATTCAACTCATGCACATGCTCTTGTTCACATTCTACTAGCTAGTTATCTAAAACTTAATATAACATGCACTTTAGGGTTGGCGAATTTAATATAACTCATGCACATGGTCTTTGTCTTTTATTACCAAAAAAAGAAAGGGAGTTTTCCCTAGAAAATACGATTTAATTTTTAATTGCCAATTTTGTATTCTGTAGTCTTAATATATATAAAATTGTCTGGCCAATGCCTCGTGTTGGCTGCACAGTGAATAGCAATATTTTTTATTTTTTCTCTTTTATTCTCACTTTCTCTTTTTGATTTTTAAAAAACCCTAGACTCTAGTATTATGATAAATCTCCTCACTAACGGTATGCCACGTGCCTGTCAAAGAAAAATAGTATGCCACGCGTATTAATTTCCCACTCTAACATTAATCATTAATATGTGCGCAAACCAAGAGCATCTTAAATTGATGATTTATTTGTTTGTTACACTTACAAGAACAGGTTACAGTATCCAGCTTGCTTTACTGAGAGGGTTTTGCTAATTAGTGTTTTTAGATATTGGTTAACGGAGTTAAAAAAATATATTTATTATAAAATTATAGACAAATATAAAAAAGTCATAAAAAATATAATTTTATATATTTTAACAAAAAAATTAGTTTCTCAATCAATACTTCAAAAATATTGATTAACATTTACCTTATTTCAATACTTCTCATTCAACCATATATATACATGGGATAATATTAAATTAAAAAAATATTTATTATAAAAATTATATATAAATATAAAAAAAGTCATAAACAACACAATTTTGCATGTATTAGCAAAAATAATAATTTCTTAATCAATGCTTCAAAATATTAGTTAACATTTACCTTATTTCAATACTTCTCATTCAACCATATATATACATGGGATAATATTAAATTAATTTAACTTTTTTAACTAGTTTGGCTAAATCAAGCTTATTTAATGTCTATGATAGTACTTTGGAAATTGGAAGATGCATGTATAATAATTTTAACGTATCAATAACCAAATTAAAAATTAGGTCAAAAGTTTGAGAAGGCAGAAAGAGCATACAAATTTCATATGATTTATAAATAATAGCTTAGTTCAGATAAACTTATTAATAATTTTAACATATGATTTTATCTTATATTTCATATTGTACAATGCAATATAATGTATTTTGC

CTTCATAATGAATGATATTACTTGTAGTCTACCAACATCATTTCATGTCAGTGTTATTTCTTATCATTTAATGATAATTGAGCCTATCTCATTTTCCACACACTTTTATTTTGATTTGGATCAAAATTAGTTACCTAAAAACTACACAAATACTTAAAAAAAAATGTTCACGAGTTAACTATGAGTAAAATATCTAATGATTGTATCACACACAAGTGACACAACCTGAAATTAATTTCCTTGAACTGATTGATAGGGATAGTATTATACACTACAGTTTGATGAT

ACTTTTAGTTTTTTTTTAATACAACACTTTTCTTAGGGTTACATTGCATCATATAGGCT

ATCTCCTTCAAAAACTAAGAATTAGTTAATCTAAAAAGGTTAAAATGTTCATTTGATTTTTATAGTTGTTCTCATTTTAAAAGTTTCGTCCATGTTCAGAAGGAAAAAAAAAACAATAGCTTTTAGTCCCTACACAATTTTTTGTGGCAGTTTATCACTACTTTTTGTAACTGTCAAAAATAAGTTTTGATATTCTCAGCAAGTACCAAAACTTAACTTAAACACATTGTAGGGACTAAAACTTATTATTTTTCGTATAAAAACTAAAACTTAAAATGAAGAGAACTGTAGAGATCAAATGAGTAGTTTAACATTAAGAAAAGAAGAATATGATTTATTACA

SEQ ID NO: 57: XP_003524313.1 AAP 4-like [Glycine max] (protein)

SEQ ID NO: 58: XP_003524313.1 AAP 4-like [Glycine max] (genomic)ATGCCTGAAAACGCAGCCACAACCAACCTTAACCACCTTCAAGTTTTTGGCATCGAAGATGATGTGCCTTCGCATTCACAGAATAACTCCAAATGCTATGATGATGATGGCCGTCTCAAACGAACTGGAAATGTTTGGACTGCAAGCTCGCACATAATAACCGCAGTGATAGGATCAGGGGTGCTGTCATTAGCTTGGGCCATAGCTCAGCTAGGTTGGATTGCTGGTCCTACTGTCATGTTCTTATTCTCTTTGGTTACCTTTTATACTTCATCCTTGTTGGCTGATTGTTATCGTGCCGGTGACCCCAATTCTGGCAAGAGAAACTACACTTACATGGACGCAGTTCGCTCCATTCTTGGTGGAGCCAATGTTACGTTGTGCGGAATATTTCAGTACCTGAATCTATTGGGAATTGTAATAGGATACACAATTGCCGCTTCTATTAGCATGATGGCAATTAAAAGGTCAAACTGTTTCCATAAATCTGGGGGCAAAAACCCATGTCACATGTCAAGCAACGTATACATGATCATTTTTGGCGCAACCGAAATTTTCCTTTCTCAAATTCCCGATTTTGATCAATTATGGTGGCTCTCAACAGTTGCTGCAATAATGTCTTTCACCTATTCCATAATTGGTCTCTCTCTTGGAATTGCCAAAGTTGCAGAAACGGGTACCTTCAAGGGTGGCCTCACTGGAATCAGCATTGGACCAGTGTCAGAGACCCAAAAAATCTGGAGGACTTCCCAAGCTCTTGGTGATATAGCCTTTGCCTATTCATATGCTGTGGTTCTTATAGAAATTCAGGACACAATAAAATCTCCACCGTCTGAAGCAAAAACAATGAAGAAGGCCACATTGATAAGTATTGCAGTGACCACAACATTTTACATGCTCTGTGGCTGCATGGGGTATGCTGCTTTTGGAGATGCTGCACCGGGGAATCTGCTAACTGGCTTTGGCTTCTATAACCCATATTGGCTTATAGACATTGCAAATGCAGCTATAGTTATTCACCTTGTGGGAGCATACCAAGTGTTTTCCCAACCCATCTTTGCCTTTGTGGAGAAAGAGGTAACACAAAGATGGCCCCACATTGAGAGGGAGTTCAAGATTCCAATTCCTGGTTTCTCCCCTTACAAACTTAAGGTGTTTAGATTAGTTTTGAGGACAGTGTTTGTTGTCCTAACAACTGTCATATCAATGCTGCTTCCATTCTTCAATGACA

GAGATGTATATTTCACAGAAGAAGATCCCAAAATGGAGTAACAGATGGATTAGCCTCAAAATATTTAGTGTGGCCTGCCTCATAGTATCAGTTGTTGCTGCTGTTGGCTCAGTGGCAGGAGTCTTGCTTGACCTTAAGAAATACAAACCATTCCACTCACACTATTAASEQ ID NO: 59: XP_006581782.1 AAP3 isoform X1 [Glycine max] (protein)MVEKSSRTNLSHHQDFGMEPYSIDGVSSQTNSKFYDDDGHVKRTGTVWTTSSSHIITAVVGSGVLSLAWAMAQMGWVAGPAVMIFFSVVTLYTTSLLADCYRCGDPVTGKRNYTFMDAVQSILGGYYDAFCGVVQYSNLYGTAVGYTIAASISMMAIKRSNCFHSSGGKSPCQVSSNPYMIGFGIIQILFSQIPDFHETWWLSIVAAIMSFVYSTIGLALGIAKVAEMGTFKGSLTGVRIGTVTEATKVWGVFQGLGDIAFAYSYSQILIEIQDTIKSPPSEAKTMKKSAKISIGVTTTFYMLCGFMGYAAFGDSAPGNLLTGFGFFNPYWLIDIANAAVIHLVGAYQVYAQPLFAFVEKWASKRWPEVETEYKIPIPGFSPYNLSPFRLVWRTVFVIITTFVAMLIPFFND

SIVLDLQKYKPFHVDYSEQ ID NO: 60: XP_006581782.1 AAP3 isoform X1 [Glycine max] (genomic)ATGGTTGAAAAATCTTCCAGAACCAATCTTAGCCACCATCAAGACTTTGGCATGGAGCCTTACTCCATTGATGGTGTTTCTTCACAAACTAACTCCAAATTCTACGATGATGATGGCCATGTTAAACGAACAGGGACCGTTTGGACAACAAGCTCGCACATAATAACAGCAGTGGTGGGTTCTGGGGTGCTGTCTTTGGCATGGGCCATGGCTCAAATGGGTTGGGTTGCTGGGCCTGCAGTTATGATCTTCTTCAGTGTTGTTACGTTGTATACGACGTCGCTTCTGGCTGATTGTTATCGCTGTGGTGACCCTGTTACCGGGAAGAGAAACTATACTTTCATGGATGCAGTTCAATCCATTCTCGGTGGGTATTATGATGCATTTTGTGGGGTAGTTCAGTACTCAAATCTTTACGGAACCGCCGTAGGATACACAATTGCAGCTTCTATTAGCATGATGGCAATAAAAAGGTCCAACTGTTTCCATTCTTCAGGCGGAAAAAGTCCATGTCAGGTTTCAAGCAACCCATACATGATCGGTTTTGGCATAATCCAAATTTTATTTTCTCAAATTCCAGATTTTCATGAAACATGGTGGCTCTCCATAGTTGCAGCAATCATGTCTTTTGTCTATTCCACAATTGGGCTCGCTCTTGGCATTGCCAAAGTTGCAGAAATGGGTACTTTCAAGGGTAGTCTCACAGGAGTAAGGATTGGAACTGTGACCGAGGCCACAAAAGTATGGGGGGTTTTCCAAGGTCTTGGTGACATAGCCTTCGCCTATTCATATTCTCAAATTCTCATTGAAATTCAGGACACCATAAAATCTCCACCATCGGAAGCAAAGACAATGAAGAAGTCTGCTAAGATAAGTATTGGAGTAACCACAACATTTTATATGCTTTGTGGTTTCATGGGCTATGCTGCTTTTGGAGATTCAGCACCTGGGAACCTGCTCACAGGATTTGGTTTTTTTAACCCATATTGGCTCATAGATATTGCTAATGCTGCTATCGTAATTCACCTTGTGGGAGCATACCAAGTTTATGCCCAGCCCCTCTTTGCCTTTGTCGAGAAATGGGCTTCAAAAAGATGGCCTGAAGTTGAGACGGAATATAAAATTCCAATTCCTGGTTTTTCACCCTACAATCTAAGCCCATTTAGATTAGTTTGGAGAACAGTGTTTGTTATCATAACCACTTTTGTAGCAATGTTGATTCCATTCTT

TCCCAGTGCAGATGAGTATCAAACAAAAGAGGACCCCAAGGTGGAGTGGTAGATGGATTGGTATGCAAATCTTAAGTGTTGTTTGTTTCATAGTATCAGTTGCGGCTGCTGTTGGCTCAGTTGCCAGTATCGTGCTTGACCTACAGAAATACAAACCGTTTCATGTA GACTATTAASEQ ID NO: 61: XP_006581783.1 AAP3 isoform X2 [Glycine max] (protein)MEPYSIDGVSSQTNSKFYDDDGHVKRTGTVWTTSSHIITAVVGSGVLSLAWAMAQMGWVAGPAVMIFFSVVTLYTTSLLADCYRCGDPVTGKRNYTFMDAVQSILGGYYDAFCGVVQYSNLYGTAVGYTIAASISMMAIKRSNCFHSSGGKSPCQVSSNPYYMIGFGIIQILFSQIPDFHETWWLSIVAAIMSFVYSTIGLALGIAKVAEMGTFKGSLTGVRIGTVTEATKVWGVFQGLGDIAFAYSYSQILIEIQDTIKSPPSEAKTMKKSAKISIGVTTTFYMLCGFMGYAAFGDSAPGNLLTGFGFFNPYWLIDIANAAIVIHLVGAYQVYAQPLFAFVEKWASKRWP

LPVQMSIKQKRTPRWSGRWIGMQILSVVCFIVSVAAAVGSVASIVLDLQKYKPFHVDYSEQ ID NO: 62: XP_006581783.1 AAP3 isoform X2 [Glycine max] (genomic)ATGGAGCCTTACTCCATTGATGGTGTTTCTTCACAAACTAACTCCAAATTCTACGATGATGATGGCCATGTTAAACGAACAGGGACCGTTTGGACAACAAGCTCGCACATAATAACAGCAGTGGTGGGTTCTGGGGTGCTGTCTTTGGCATGGGCCATGGCTCAAATGGGTTGGGTTGCTGGGCCTGCAGTTATGATCTTCTTCAGTGTTGTTACGTTGTATACGACGTCGCTTCTGGCTGATTGTTATCGCTGTGGTGACCCTGTTACCGGGAAGAGAAACTATACTTTCATGGATGCAGTTCAATCCATTCTCGGTGGGTATTATGATGCATTTTGTGGGGTAGTTCAGTACTCAAATCTTTACGGAACCGCCGTAGGATACACAATTGCAGCTTCTATTAGCATGATGGCAATAAAAAGGTCCAACTGTTTCCATTCTTCAGGCGGAAAAAGTCCATGTCAGGTTTCAAGCAACCCATACATGATCGGTTTTGGCATAATCCAAATTTTATTTTCTCAAATTCCAGATTTTCATGAAACATGGTGGCTCTCCATAGTTGCAGCAATCATGTCTTTTGTCTATTCCACAATTGGGCTCGCTCTTGGCATTGCCAAAGTTGCAGAAATGGGTACTTTCAAGGGTAGTCTCACAGGAGTAAGGATTGGAACTGTGACCGAGGCCACAAAAGTATGGGGGGTTTTCCAAGGTCTTGGTGACATAGCCTTCGCCTATTCATATTCTCAAATTCTCATTGAAATTCAGGACACCATAAAATCTCCACCATCGGAAGCAAAGACAATGAAGAAGTCTGCTAAGATAAGTATTGGAGTAACCACAACATTTTATATGCTTTGTGGTTTCATGGGCTATGCTGCTTTTGGAGATTCAGCACCTGGGAACCTGCTCACAGGATTTGGTTTTTTTAACCCATATTGGCTCATAGATATTGCTAATGCTGCTATCGTAATTCACCTTGTGGGAGCATACCAAGTTTATGCCCAGCCCCTCTTTGCCTTTGTCGAGAAATGGGCTTCAAAAAGATGGCCTGAAGTTGAGACGGAATATAAAATTCCAATTCCTGGTTTTTCACCCTACAATCTAAGCCCATTTAGATTAGTTTGGAGAACAGTGTTTGTTATCATAACCACTTTTGTAGCAATGTTGATTCCATT

TTCTCCCAGTGCAGATGAGTATCAAACAAAAGAGGACCCCAAGGTGGAGTGGTAGATGGATTGGTATGCAAATCTTAAGTGTTGTTTGTTTCATAGTATCAGTTGCGGCTGCTGTTGGCTCAGTTGCCAGTATCGTGCTTGACCTACAGAAATACAAACCGTTTCAT GTAGACTATTAAMAIZESEQ ID NO: 63: NP_001136620 uncharacterized protein LOC100216745 [Zea mays]AQL04004.1: (protein)MVSERQQAAGKVAAFNLTEAGFGDGSDLLDDDGRERRTGTVTASAHIITAVIGSSVLSLAWAIAQLGWVIGPVVLLAFSAITWFCSSLLADCYRAPPGPGQGKRNYTYGQAVRSYLGESKYRLCSLAQYVNLVGVTIGYTITTAISMGAIKRSNCFHSRGHGSDCEASNTTNMIIFAGIQILLSQLPNFHKWWSIVAAVMSAYSSIGLLGSIAKIAGGVHVKTSTGAAVGVDVTAAEKVWKTFQSLGDIAFAYTYSNVLIEIQDTLRSSPPENVVMKKASFIGVSTTTAFYMLCGVLGYAAFGSDAPGNFLTGFGFYDPFWLIDVGNVCIAVHLVGAYQVFCQPIYQFVEAWARGRWPDCAFLHAELAVVAGSSFTASPFRLVWRTAYVVLTALVATVFPFFN

VQGLVKDLKGYKPLFKVSSEQ ID NO: 64: NP_001136620 uncharacterized protein LOC100216745 [Zea mays](genomic) ATGGTGTCGGAGAGGCAGCAGGCGGCGGGGAAGGTGGCCGCCTTCAACCTCACGGAGGCCGGGTTCGGCGACGGGTCGGACCTGCTGGACGACGACGGGCGCGAGAGGCGCACGGGGACCCTGGTGACGGCGAGCGCGCACATCATCACGGCGGTGATCGGGTCGAGCGTGCTGTCGCTGGCGTGGGCGATCGCGCAGCTGGGGTGGGTGATCGGCCCCGTGGTGCTGCTGGCCTTCTCCGCCATCACCTGGTTCTGCTCCAGCCTACTCGCCGACTGCTACCGCGCGCCGCCGGGCCCCGGCCAGGGCAAGCGGAACTACACCTACGGACAGGCCGTCAGGTCATACCTGGGGGAGTCCAAGTACCGGCTGTGCTCGCTGGCGCAGTACGTGAACCTGGTGGGCGTCACCATCGGCTACACCATCACCACGGCCATCAGCATGGGGGCGATCAAGCGTTCCAACTGCTTCCACAGCAGGGGCCACGGCGCCGACTGCGAGGCGTCCAACACCACCAACATGATCATCTTCGCGGGCATCCAGATCCTGCTGTCGCAGCTCCCCAACTTCCACAAGCTCTGGTGGCTCTCCATCGTCGCCGCCGTCATGTCCCTCGCCTACTCCTCCATCGGACTCGGCCTCTCCATCGCAAAGATCGCAGGTGGGGTGCACGTTAAGACGTCGCTGACTGGTGCCGCCGTGGGGGTGGACGTCACCGCGGCCGAGAAGGTCTGGAAGACGTTCCAGTCGCTGGGGGACATCGCCTTCGCCTACACCTACTCCAACGTGCTGATCGAGATCCAGGACACGCTGCGGTCGAGCCCGCCGGAGAACGTGGTGATGAAGAAGGCGTCCTTCATCGGCGTGTCCACCACCACCGCGTTCTACATGCTGTGCGGCGTGCTGGGCTACGCGGCGTTCGGCAGCGACGCGCCGGGCAACTTCCTCACGGGCTTCGGCTTCTACGACCCCTTCTGGCTCATCGACGTCGGCAACGTCTGCATCGCCGTGCACCTGGTCGGCGCCTACCAGGTCTTCTGCCAGCCCATCTACCAGTTCGTGGAGGCCTGGGCGCGGGGCCGCTGGCCCGACTGCGCCTTCCTCCACGCCGAGCTCGCCGTCGTCGCCGGCTCCTCCTTCACGGCCAGCCCGTTCCGCCTCGTGTGGCGCACCGCCTACGTCGTGCTCACCGCGCTCGTCGCCACGGTCTTCCCATTCTTCAACGACTTCCTGGGG

ACATGGCGCAGGCCAAGACGCGCCGCTTCTCGCCGGCGTGGACGTGGATGAACGTGCTCAGCTACGCTTGCCTCTTCGTCTCGCTGCTCGCCGCCGCGGGCTCAGTGCAGGGGCTCGTCAAGGATCTCAAGGGATACAAGCCATTGTTCAAGGTCTCCTAASEQ ID NO: 65: PWZ15603 AAP6 [Zea mays] (protein)MVSERQQAAGKVAAFNLTEAGFGDGSDLLDDDGRERRTGTLVTASAHIITAVIGSGVLSLAWAIAQLGWVIGPVVLLAFSSAITWFCSSLLADCYRAPPGPGQGKRNYTYGQAVRSYLGESKYRLCSLAQYVNLVGVTIGYTITTAISMGAIKRSNCFHSRGHGADCEASNTTNMIIFAGIQILLSQLPNFHKLWWLSIVAAVMSLAYSSIGLGLSIAKIAGKLMHGSGVHVKTSLTGAAVGVDVTAAEKVWKTFQSLGDIAFAYTYSNVLIEIQDTLRSSPPENVVMKKASFIGVSTTTAFYMLCGVLGYAAFGSDAPGNFLTGFGFYDPFWLIDVGNVCIAVHLVGAYQVFCQPIYQFVEAWARGRWPDCAFLHAELAVVAGSSFTASPFRLVWRTAYVVLTALVA

LLAAAGSVQGLVKDLKGYKPLFKVSSEQ ID NO: 66: PWZ15603 AAP6 [Zea mays] (genomic)ATGGTGTCGGAGAGGCAGCAGGCGGCGGGGAAGGTGGCCGCCTTCAACCTCACGGAGGCCGGGTTCGGCGACGGGTCGGACCTGCTGGACGACGACGGGCGCGAGAGGCGCACGGGGACCCTGGTGACGGCGAGCGCGCACATCATCACGGCGGTGATCGGGTCGGGCGTGCTGTCGCTGGCGTGGGCGATCGCGCAGCTGGGGTGGGTGATCGGCCCCGTGGTGCTGCTGGCCTTCTCCGCCATCACCTGGTTCTGCTCCAGCCTACTCGCCGACTGCTACCGCGCGCCGCCGGGCCCCGGCCAGGGCAAGCGGAACTACACCTACGGACAGGCCGTCAGGTCATACCTGGGGGAGTCCAAGTACCGGCTGTGCTCGCTGGCGCAGTACGTGAACCTGGTGGGCGTCACCATCGGCTACACCATCACCACGGCCATCAGCATGGGGGCGATCAAGCGTTCCAACTGCTTCCACAGCAGGGGCCACGGCGCCGACTGCGAGGCGTCCAACACCACCAACATGATCATCTTCGCGGGCATCCAGATCCTGCTGTCGCAGCTCCCCAACTTCCACAAGCTCTGGTGGCTCTCCATCGTCGCCGCCGTCATGTCCCTCGCCTACTCCTCCATCGGACTCGGCCTCTCCATCGCAAAGATCGCAGGCAAGCTCATGCATGGCAGTGGGGTGCACGTTAAGACGTCGCTGACTGGTGCCGCCGTGGGGGTGGACGTCACCGCGGCCGAGAAGGTCTGGAAGACGTTCCAGTCGCTGGGGGACATCGCCTTCGCCTACACCTACTCCAACGTGCTGATCGAGATCCAGGACACGCTGCGGTCGAGCCCGCCAGAGAACGTGGTGATGAAGAAGGCGTCCTTCATCGGCGTGTCCACCACCACCGCGTTCTACATGCTGTGCGGCGTGCTGGGCTACGCGGCGTTCGGCAGCGACGCGCCGGGCAACTTCCTCACGGGCTTCGGCTTCTACGACCCCTTCTGGCTCATCGACGTCGGCAACGTCTGCATCGCCGTGCACCTGGTCGGCGCCTACCAGGTCTTCTGCCAGCCCATCTACCAGTTCGTGGAGGCCTGGGCGCGGGGCCGCTGGCCCGACTGCGCCTTCCTCCACGCCGAGCTCGCCGTCGTCGCCGGCTCCTCCTTCACGGCCAGCCCGTTCCGCCTCGTGTGGCGCACCGCCTACGTCGTGCTCACCGCGCTCGTCGCCACGGTCTTCCCATTCT

CTTCCCCATCCAGATGTACATGGCGCAGGCCAAGACGCGCCGCTTCTCGCCGGCGTGGACGTGGATGAACGTGCTCAGCTACGCTTGCCTCTTCGTCTCGCTGCTCGCCGCCGCGGGCTCCGTGCAGGGGCTCGTCAAGGATCTCAAGGGATACAAGCCATT GTTCAAGGTCTCCTAASEQ ID NO: 67: ONM51229.1 Amino acid permease 6 [Zea mays] (protein)MVSERQQAAGKVAAFNLTEAGFGDGSDLLDDDGRERRTGTLVTASAHIITAVIGSGVLSLAWAIAQLGWVIGPVVLLAFSAITWFCSSLLADCYRAPPGPGQGKRNYTYGQAVRSYLGESKYRLCSLAQYVNLVGVTIGYTITTAISMGAIKRSNCFHSRGHGADCEASNTTNMIIFAGIQILLSQLPNFHKLWWLSIVAAVMSLAYSSIGLGLSIAKIAGKLMHGSYCGVHVKTSLTGAAVGVDVTAAEKVWKTFQSLGDIAFAYTYSNVLIEIQDTLRSSPPENVVMKKASFIGVSTTTAFYMLCGVLGYAAFGSDAPGNFLTGFGFYDPFWLIDVGNVCIAVHLVGAYQVFCQPIYQFVEAWARGRWPDCAFLHAELAVVAGSSFTASPFRLVWRTAYVVLTAL

VSLLAAAGSVQGLVKDLKGYKPLFKVSSEQ ID NO: 68: ONM51229.1 Amino acid permease 6 [Zea mays] (genomic)ATGGTGTCGGAGAGGCAGCAGGCGGCGGGGAAGGTGGCCGCCTTCAACCTCACGGAGGCCGGGTTCGGCGACGGGTCGGACCTGCTGGACGACGACGGGCGCGAGAGGCGCACGGGGACCCTGGTGACGGCGAGCGCGCACATCATCACGGCGGTGATCGGGTCGGGCGTGCTGTCGCTGGCGTGGGCGATCGCGCAGCTGGGGTGGGTGATCGGCCCCGTGGTGCTGCTGGCCTTCTCCGCCATCACCTGGTTCTGCTCCAGCTACTCGCCGACTGCTACCGCGCGCCGCCGGGCCCCGGCCAGGGCAAGCGGAACTACACCTACGGACAGGCCGTCAGGTCATACCTGGGGGAGTCCAAGTACCGGCTGTGCTCGCTGGCGCAGTACGTGAACCTGGTGGGCGTCACCATCGGCTACACCATCACCACGGCCATCAGCATGGGGGCGATCAAGCGTTCCAACTGCTTCCACAGCAGGGGCCACGGCGCCGACTGCGAGGCGTCCAACACCACCAACATGATCATCTTCGCGGGCATCCAGATCCTGCTGTCGCAGCTCCCCAACTTCCACAAGCTCTGGTGGCTCTCCATCGTCGCCGCCGTCATGTCCCTCGCCTACTCCTCCATCGGACTCGGCCTCTCCATCGCAAAGATCGCAGGCAAGCTCATGCATGGCAGCTACTGTGGGGTGCACGTTAAGACGTCGCTGACTGGTGCCGCCGTGGGGGTGGACGTCACCGCGGCCGAGAAGGTCTGGAAGACGTTCCAGTCGCTGGGGGACATCGCCTTCGCCTACACCTACTCCAACGTGCTGATCGAGATCCAGGACACGCTGCGGTCGAGCCCGCCGGAGAACGTCTGTGCGGCGTGCTGGGCTACGCGGCGTTCGGCAGCGACGCGCCGGGCAACTTCCTCACGGGCTTCGGCTTCTACGACCCCTTCTGGCTCATCGACGTCGGCAACGTCTGCATCGCCGTGCACCTGGTCGGCGCCTACCAGGTCTTCTGCCAGCCCATCTACCAGTTCGTGGAGGCCTGGGCGCGGGGCCGCTGGCCCGACTGCGCCTTCCTCCACGCCGAGCTCGCCGTCGTCGCCGGCTCCTCCTTCACGGCCAGCCCGTTCCGCCTCGTGTGGCGCACCGCCTACGTCGTGCTCACCGCGCTCGTCGCCACGGTCTTCCC

GTCTACTTCCCCATCCAGATGTACATGGCGCAGGCCAAGACGCGCCGCTTCTCGCCGGCGTGGACGTGGATGAACGTGCTCAGCTACGCTTGCCTCTTCGTCTCGCTGCTCGCCGCCGCGGGCTCAGTGCAGGGGCTCGTCAAGGATCTCAAGGGATACAAGCCATTGTTCAAGGTCTCCTAASEQ ID NO: 69: NP_001349744.1 uncharacterized protein LOC100501686 [Zea mays](protein)MTQQDVEMAARHGTGADGAGFYPQPRNGAGGETLDDDGKKKRTGTVWTASAHIITAVIGSGVLSLAWSTAQLGWVVGPLTLMIFALITYYTSSSLLADCYRSGDQLTGKRNYTYMDAVAAYLGRWQVLSCGVFQYVNLVGTAVGYTITASISAAAVHKANCFHNKGHAADCSTYDTMYMVVFGIVQIFFSQLPNFSDLSWLSIVAAIMSFSYSSIAVGLSLARTISGRSGTTTLTGTEIGVDVDSAQKVWLALQALGNIAFAYSYSMILIEIQDTVKSPPAENKTMKKATLMGVTTTTAFYMLAGCLGYSAFGNAAPGNILTGFGFYEPYWLIDFANVCIVVHLVGAYQVFSQPIFAALETAAAKRWPNARFVTREHPLVAGRFHVNLLRLTWRTAFVVVSTVLAIVL

ASIEGVTESLKHYVPFKTKSSEQ ID NO: 70: NP_001349744.1 uncharacterized protein LOC100501686 [Zea mays](genomic)ATGACGCAGCAGGACGTGGAGATGGCGGCGCGCCACGGGACCGGCGCCGACGGAGCGGGATTCTACCCTCAGCCGCGGAACGGCGCCGGCGGCGAGACGCTCGACGACGACGGCAAGAAGAAGCGCACGGGAACGGTATGGACGGCAAGCGCGCACATCATCACAGCCGTCATCGGCTCCGGCGTGCTCTCCCTCGCCTGGTCGACTGCACAGCTGGGCTGGGTCGTGGGGCCGCTCACCCTGATGATCTTTGCCTTGATCACGTACTACACCTCTAGCCTTCTTGCTGACTGCTACCGCAGCGGCGATCAGCTCACCGGCAAGAGGAACTACACCTACATGGACGCTGTTGCCGCGTACCTGGGTCGATGGCAAGTCCTGTCCTGTGGTGTTTTCCAGTATGTTAACTTGGTTGGAACTGCCGTTGGGTATACAATTACAGCGTCCATCAGTGCAGCGGCCGTGCACAAGGCAAACTGCTTCCACAACAAGGGCCACGCGGCCGACTGCAGCACCTACGACACCATGTACATGGTCGTATTTGGGATCGTTCAGATCTTCTTCTCTCAGCTCCCTAACTTCAGCGACCTTTCGTGGCTGTCCATCGTCGCCGCCATCATGTCGTTCTCTTACTCCAGCATCGCCGTCGGCCTCTCGTTGGCGCGGACCATTTCAGGCCGTAGTGGTACGACCACTCTGACCGGCACTGAGATCGGAGTCGACGTTGATTCAGCCCAGAAGGTCTGGCTCGCGCTTCAAGCTCTTGGCAACATCGCGTTCGCTTACTCCTACTCCATGATTCTCATCGAAATCCAAGACACGGTGAAGTCTCCTCCAGCCGAGAACAAGACGATGAAGAAGGCGACGCTGATGGGCGTGACGACCACCACGGCGTTCTACATGCTTGCTGGCTGCCTCGGGTACTCGGCATTCGGGAACGCGGCGCCAGGGAACATCCTGACCGGGTTCGGCTTCTACGAGCCCTACTGGCTGATCGACTTCGCCAACGTCTGCATCGTGGTGCACCTGGTGGGCGCGTACCAGGTCTTCTCCCAGCCCATCTTCGCGGCCTTGGAGACGGCGGCCGCCAAGCGCTGGCCGAACGCCAGGTTCGTCACGCGCGAGCACCCCCTCGTGGCCGGCAGGTTCCACGTCAACCTGCTCAGGCTGACGTGGAGGACGGCGTTCGTGGTGGTGAGCACGGTGCTCGCCATCGTGTTGCCCTTCTTCAACGATATCCTGGGC

ACATCCGGCAGCGGCGTATACAGAAGTACACCAGCAGGTGGGTGGCGCTGCAGCTGCTCAGCTTCCTGTGCTTCCTGGTCTCGCTCGCCTCGGCGGTCGCGTCCATCGAGGGAGTCACCGAGTCGCTCAAACACTACGTTCCCTTTAAGACCAAGTCGTGASEQ ID NO: 71: PWZ08709 AAP3 [Zea mays] (protein)MEVSSVEFGHAAAASKCFDDDGRLKRTGTMWTASAHIITAVIGSGVLSLAWAIAQLGWVAGPTVMLLFSFVTYYTSALLADCYRSGDACTGKRNYTYMDAVNANLSGVKVWFCGFLQYANIVGVAIGYTIAASISMLAIQRANCFHVEGHGDPCNISSTPYMIIFGVVQIFFSQIPDFDQISWLSILAAVMSFTYSTIGLGLGIAQVVSNKGVQGSLTGISVGAVTPVDKMWRSLQAFGDIAFAYSYSLILIEIQDTIRAPPPSESKVMRRATVVSVAVTTFFYMLCGCMGYAAFGDNAPGNLLTGFGFYEPFWLLDVANAAIAVHLVGAYQVYCQPLFAFVEKWARQRWPKSRYITGEVDVPLPLGTAAGRCYKLSLFRLTWRTAFVVATTVVSMLLPFFNDVVGL

DLKVYKPFVTTS SEQ ID NO: 72: PWZ08709.1 AAP3 [Zea mays] (protein)ATGGAGGTGAGCTCCGTGGAGTTCGGTCACGCGGCGGCCGCCTCAAAGTGCTTTGACGACGACGGTCGCCTCAAGCGCACAGGGACGATGTGGACGGCGAGCGCGCACATTATCACGGCCGTGATAGGGTCCGGGGTGCTGTCGCTCGCGTGGGCCATCGCGCAGCTCGGCTGGGTGGCAGGCCCCACCGTCATGCTGCTCTTCTCCTTCGTCACCTACTACACGTCGGCCCTACTCGCCGACTGCTACCGCTCCGGCGACGCCTGCACCGGCAAGCGCAACTACACGTACATGGACGCGGTTAACGCCAATCTCAGTGGCGTCAAGGTTTGGTTCTGCGGGTTCCTGCAGTACGCCAACATCGTCGGAGTCGCCATAGGCTACACCATTGCCGCCTCTATTAGCATGCTGGCGATCCAGAGGGCGAACTGCTTCCACGTGGAGGGGCACGGGGACCCCTGCAACATCTCCAGCACGCCCTACATGATCATCTTCGGCGTCGTGCAGATTTTCTTCTCGCAGATCCCGGACTTCGACCAGATATCGTGGCTCTCCATCCTCGCCGCCGTCATGTCCTTCACCTACTCCACCATCGGCCTGGGCCTGGGCATCGCGCAGGTGGTGTCCAACAAGGGCGTGCAGGGCAGCCTGACGGGGATCAGCGTCGGCGCGGTCACCCCGGTCGACAAGATGTGGCGCAGCCTGCAGGCGTTCGGCGACATCGCCTTCGCCTACTCCTACTCCCTCATCCTCATCGAGATCCAGGACACCATCCGCGCGCCGCCGCCGTCCGAGTCCAAGGTCATGCGGCGCGCCACCGTCGTCAGCGTGGCCGTCACCACGTTCTTCTACATGCTGTGCGGGTGCATGGGGTACGCCGCGTTCGGGGACAACGCCCCCCGGGAACCTCCTCACGGGCTTCGGCTTCTACGAGCCCTTCTGGCTCCTCGACGTCGCCAACGCCGCCATCGCCGTGCACCTCGTCGGCGCCTACCAGGTCTACTGCCAGCCACTGTTCGCCTTCGTCGAGAAGTGGGCGCGCCAGAGGTGGCCCAAGTCCCGCTACATCACGGGCGAGGTCGACGTCCCGCTCCCGCTCGGGACCGCCGCCGGCCGGTGCTACAAGCTCAGCCTGTTCCGGCTGACGTGGCGGACGGCGTTCGTGGTGGCCACGACGGTGGTGTCC

TGGCCGCTCACCGTCTACTTCCCCGTGGAGATGTACATCGTGCAGAAGAAGGTGCCCAGGTGGAGCACGCGGTGGGTGTGCCTGCAGCTGCTCAGCGTCGCCTGCCTCGTCATCACCGTCGCCTCCGCCGCAGGCTCCGTTGCCGGGATCGTCTCTGACCTCAAAGTGTACAAACCGTTCGTCACCACCTCCTGASEQ ID NO: 73: NP_001149036 amino acid carrier [Zea mays] (protein)MEVSSVEFGHHAAAASKCFDDDGRLKRTGTMWTASAHIITAVIGSGVLSLAWAIAQLGWVAGPTVMLLFSFVTYYTSALLADCYRSGDACTGKRNYTYMDAVNANLSGVKVWFCGFLQYANIVGVAIGYTIAASISMLAIQRANCFHVEGHGDPCNISSTPYMIIFGVVQIFFSQIPDFDQISWLSILAAVMSFTYSTIGLGLGIAQVVSNKGVQGSLTGISVGLVTPVDKMWRSLQAFGDIAFAYSYSLILIEIQDTIRAPPPSESKVMRRATVVSVAVTTFFYMLCGCMGYAAFGDNAPGNLLTGFGFYEPFWLLDVANAAIAVHLVGAYQVYCQPLFAFVEKWARQRWPKSRYITGEVDVPLPLGTAGGRCYKLSLFRLTWRTAFVVATTVVSMLLPFFNDVVGL

DLKVYKPFVTTSSEQ ID NO: 74: NP_001149036 amino acid carrier [Zea mays] (genomic)ATGGAGGTGAGCTCCGTGGAGTTCGGTCATCACGCGGCGGCCGCCTCAAAGTGCTTTGACGACGACGGTCGCCTCAAGCGCACAGGGACGATGTGGACGGCGAGCGCGCACATTATCACGGCCGTGATAGGGTCCGGGGTGCTGTCGCTCGCGTGGGCCATCGCGCAGCTCGGCTGGGTGGCAGGCCCCACCGTCATGCTGCTCTTCTCCTTCGTCACCTACTACACATCGGCCCTACTCGCCGACTGCTACCGCTCCGGCGACGCCTGCACCGGCAAGCGCAACTACACGTACATGGACGCGGTTAACGCCAATCTCAGTGGCGTCAAGGTCTGGTTCTGCGGGTTCCTGCAGTACGCCAACATCGTCGGAGTCGCCATAGGCTACACCATTGCCGCCTCTATTAGCATGCTGGCGATCCAGAGGGCGAACTGCTTCCACGTGGAGGGGCACGGGGACCCCTGCAACATCTCAGCACGCCCTACATGATCATCTTCGGCGTCGTGCAGATTTTCTTCTCGCAGATCCCGGACTTCGACCAGATATCGTGGCTCTCCATCCTCGCCGCCGTCATGTCGTTCACCTACTCCACCATCGGCCTGGGCCTGGGCATCGCGCAGGTGGTGTCCAACAAGGGCGTGCAGGGCAGCCTGACGGGGATCAGCGTCGGCTTGGTCACCCCGGTCGACAAGATGTGGCGCAGCCTGCAGGCGTTCGGCGACATCGCCTTCGCCTACTCCTACTCGCTCATCCTCATCGAGATCCAGGACACCATCCGCGCGCCGCCGCCGTCCGAGTCCAAGGTCATGCGGCGCGCCACCGTCGTCAGCGTGGCCGTCACCACGTTCTTCTACATGCTGTGCGGGTGCATGGGGTACGCCGCGTTCGGGGACAACGCCCCCGGGAACCTCCTCACGGGCTTCGGCTTCTACGAGCCCTTCTGGCTCCTCGACGTCGCCAACGCCGCCATCGCCGTGCACCTCGTCGGCGCCTACCAGGTCTACTGCCAGCCCCTGTTCGCCTTCGTCGAGAAGTGGGCGCGCCAGAGGTGGCCCAAGTCCCGCTACATCACGGGCGAGGTCGACGTCCCGCTCCCGCTCGGGACCGCCGGCGGCCGGTGCTACAAGCTCAGCCTGTTCCGGCTGACGTGGCGGACGGCGTTCGTGGTGGCCACGACGGTGGTGT

TCTGGCCGCTCACCGTCTACTTCCCCGTGGAGATGTACATCGTGCAGAAGAAGGTGCCCAGGTGGAGCACGCGGTGGGTGTGCCTGCAGCTGCTCAGCGTCGCCTGCCTCGTCATCACCGTCGCCTCCGCCGCAGGCTCCGTTGCCGGGATCGTCTCTGACCTCAAAGTGTACAAACCGTTCGTCACCACCTCCTGASEQ ID NO: 75: ACG33909.1 amino acid carrier [Zea mays] (protein)MEVSSVEFGHHAAAASKCFDDDGRLKRTGTMWTASAHIITAVIGSGVLSLAWAIAQLGWVAGPTVMLLFSFVTYYTSALLADCYRSGDACTGKRNYTYMDAVNANLSGVKVWFCGFLQYANIVGVAIGYTIAASISMLAIQRANCFHVEGHGDPCNISSTPYMIIFGVVQIFFSQIPDFDQISWLSILAAVMSFTYSTIGLGLGIAQVVSNKGVQGSLTGISVGAVTPVDKMWRSLQAFGDIAFAYSYSLILIEIQDTIRAPPPSESKVMRRATVVSVAVTTFXYMLCGCMGYAAFGDNAPGNLLTGFGFYEPFWLLDVANAAIAVHLVGAYQVYCQPLFAFVEKWARQRWPKSRYITGEVDVPLPLGTAGGRCYKLSLFRLTWRTAFVVATTVVSMLLPFFNDVVGL

DLKVYKPFVTTSSEQ ID NO: 76: ACG33909.1 amino acid carrier [Zea mays] (genomic)ATGGAGGTGAGCTCCGTGGAGTTCGGTCATCACGCGGCGGCCGCCTCAAAGTGCTTTGACGACGACGGTCGCCTCAAGCGCACAGGGACGATGTGGACGGCGAGCGCGCACATTATCACGGCCGTGATAGGGTCCGGGGTGCTGTCGCTCGCGTGGGCCATCGCGCAGCTCGGCTGGGTGGCAGGCCCCACCGTCATGCTGCTCTTCTCCTTCGTCACCTACTACACATCGGCCCTACTCGCCGACTGCTACCGCTCCGGCGACGCCTGCACCGGCAAGCGCAACTACACGTACATGGACGCGGTTAACGCCAATCTCAGTGGCGTCAAGGTCTGGTTCTGCGGCTTCCTGCAGTACGCCAACATCGTCGGAGTCGCCATAGGCTACACCATTGCCGCCTCTATTAGCATGCTGGCGATCCAGAGGGCGAACTGCTTCCACGTGGAGGGGCACGGGGACCCCTGCAACATCTCCAGCACGCCCTACATGATCATCTTCGGCGTCGTGCAGATTTTCTTCTCGCAGATCCCGGACTTCGACCAGATATCGTGGCTCTCCATCCTCGCCGCCGTCATGTCCTTCACCTACTCCACCATCGGCCTGGGCCTGGGCATCGCGCAGGTGGTGTCCAACAAGGGCGTGCAGGGCAGCCTGACGGGGATCAGCGTCGGCGCGGTCACCCCGGTCGACAAGATGTGGCGCAGCCTGCAGGCGTTCGGCGACATCGCCTTCGCCTACTCCTACTCCCTCATCCTCATCGAGATCCAGGACACCATCCGCGCGCCGCCGCCGTCCGAGTCCAAGGTCATGCGGCGCGCCACCGTCGTCAGCGTGGCCGTCACCACGTTCTTMTACATGCTGTGCGGGTGCATGGGGTACGCCGCGTTCGGGGACAACGCCCCCGGGAACCTCCTCACGGGCTTCGGCTTCTACGAGCCCTTCTGGCTCCTCGACGTCGCCAACGCCGCCATCGCCGTGCACCTCGTCGGCGCCTACCAGGTCTACTGCCAGCCCCTGTTCGCCTCGTCGAGAAGTGGGCGCGCCAGAGGTGGCCCAAGTCCCGCTACATCACGGGCGAGGTCGACGTCCCGCTCCCGCTCGGGACCGCCGGCGGCCGGTGCTACAAGCTCAGCCTGTTCCGGCTGACGTGGCGGACGGCGTTCGTGGTGGCCACGACGGTGGTG

TTCTGGCCGCTCACCGTCTACTTCCCCGTGGAGATGTACATCGTGCAGAAGAAGGTGCCCAGGTGGAGCACGCGGTGGGTGTGCCTGCAGCTGCTCAGCGTCGCCTGCCTCGTCATCCCGTCGCCTCCGCCGCAGGCTCCGTTGCCGGGATCGTCTCTGACCTCAAAGTGTACAAACCGTTCGTCACCACCTCCTGASEQ ID NO: 77: NP_001142349 AAP2 [Zea mays] (protein)MAENNVVATYYYPTAAPAAMEVCGAELGQGKPDKCFDDDGRPKRNGTMWTASAHIITAVIGSGVLSLLGWAIAQLGWVAGPVVMLLFSLVTYYTSSLLADCYRSGDPSTGKRNYTYMDAVNANLSGIKVQICGFLQYANIVGVAIGYTIAASISMLAIRRANCFHQKGHGNPCKISSTPYMIIFGVAEIFFSQIPDFDQISWWLSILAAVMSFTYSSIGLGLGVVQVIANRGVQGSLTGITIGVVTPMDKVWRSLQAFGDVAFAYSYSLILIEIQDTIRAPPPSESTVMKRATVVSVAVTTLFYMLCGCMGYAAFGDGAPGNLLTGFGFYEPFWLLDVANAAIVVHLVGAYQVYCQPLFAFVEKWAAQRWPDSAYITGEVEVPLPLPASRRRCCKVNLFRATWRTAFVVAT

CLVISIAAAAGSIAGIASDLKVYRPFKSYSEQ ID NO: 78: NP_001142349 AAP2 [Zea mays] (genomic)ATGGCGGAGAACAACGTCGTGGCCACGTACTACTACCCGACGGCAGCGCCGGCGGCCATGGAGGTCTGCGGCGCGGAGCTCGGCCAGGGCAAGCCCGACAAGTGCTTCGACGACGATGGCCGCCCCAAGCGCAATGGGACGATGTGGACGGCGAGCGCGCACATCATCACGGCGGTGATCGGCTCCGGGGTGCTCTCGCTGGGGTGGGCCATCGCGCAGCTCGGCTGGGTGGCCGGACCCGTCGTCATGCTGCTCTTCTCGCTCGTCACCTACTACACCTCGTCGCTGCTCGCAGACTGCTACCGCTCCGGCGACCCCAGCACCGGCAAGCGGAACTACACCTACATGGACGCCGTCAACGCGAACCTCAGTGGCATCAAGGTCCAGATCTGCGGGTTCCTGCAGTACGCCAACATCGTGGGCGTGGCCATCGGCTACACCATCGCTGCCTCCATTAGCATGCTCGCGATCAGGAGGCCAACTGCTTCCACCAGAAGGGACACGGCAACCCCTGCAAGATCTCCAGCACGCCCTACATGATCATCTTCGGCGTGGCGGAGATCTTCTTCTCGCAGATCCCGGACTTCGACCAGATCTCCTGGCTCTCCATCCTCGCCGCCGTCATGTCCTTCACCTACTCCTCCATTGGGCTCGGCCTGGGCGTCGTCCAAGTCATCGCGAACAGAGGCGTGCAGGGCAGCCTGACCGGCATCACCATCGGCGTGGTGACCCCGATGGACAAGGTGTGGCGCAGCCTCCAGGCGTTCGGCGACGTCGCCTTCGCCTACTCCTACTCCCTCATCCTGATCGAGATCCAGGACACCATCCGGGCGCCGCCGCCGTCGGAGTCGACGGTGATGAAGCGCGCCACGGTGGTGAGCGTGGCGGTCACCACGCTCTTCTACATGCTGTGCGGCTGCATGGGGTACGCGGCGTTCGGCGACGGCGCGCCCGGGAACCTCCTCACGGGCTTCGGCTTCTACGAGCCCTTCTGGCTCCTGGACGTGGCCAACGCCGCCATCGTGGTCCACCTGGTCGGCGCCTACCAGGTCTACTGCCAGCCGCTGTTCGCCTTCGTGGAGAAGTGGGCCGCGCAGCGGTGGCCGGACTCGGCGTACATCACCGGGGAGGTCGAGGTCCCGCTCCCGCTCCCGGCGAGCCGGCGGCGGTGCTGCAAGGTGAACCTGTTCCGGGCGACGTGGCGGACGGCGTTCGTCGTGGCCACGACGGTCGTGT

TCTGGCCGCTCACCGTCTACTTCCCCGTCGAGATGTACGTGGTGCAGAAGAAGGTGCCGCGGTGGAGCTCCCGGTGGGTGTGCCTGCAGATGCTCAGCCTCGGCTGCCTCGTCATCTCCATCGCCGCCGCAGCCGGGTCCATCGCCGGCATCGCGTCCGACCTCAAAGTCTACCGCCCGTTCAAGTCCTACTGASEQ ID NO: 79: PWZO7549 AAP1 [Zea mays] (protein)MTQQDVEMAARHGTGADGAGFYPQPRNGAGGETLDDDGKKKRTGVIATIGGVPSTGANVPPNVGVLDEPGTDAMPLMRPRTVWTASAHIITAVIGSGVLSLAWSTAQLGWVVGPLTLMIFALITYYTSSLLADCYRSGDQLTGKRNYTYMDAVAAYLGRWQVLSCGVFQYVNLVGTAVGYTITASISAAAVHKANCFHNKGHAADCSTYDTMYMVVFGIVQIFFSQLPNFSDLSWLSIVAAIMSFSYSSIAVGLSLARTISGRSGTTTLTGTEIGVDVDSAQKVWLALQALGNIAFAYSYSMILIEIQDTVKSPPAENKTMKKATLMGVTTTTAFYMLAGCLGYSAFGNAAPGNILTGFGFYEPYWLIDFANVCIVVHLVGAYQVFSQPIFAALETAAAKRWPNARF

VEMYIRQRRIQKYTSRWVALQLLSFLCFLVSLASAVASIEGVTESLKHYVPFKTKSSEQ ID NO: 80: PWZ07549 AAP1 [Zea mays] (genomic)ATGACGCAGCAGGACGTGGAGATGGCGGCGCGCCACGGGACCGGCGCCGACGGAGCGGGATTCTACCCTCAGCCGCGGAACGGCGCCGGCGGCGAGACGCTCGACGACGACGGCAAGAAGAAGCGCACGGGTGTAATAGCCACTATTGGAGGTGTACCAAGCACTGGTGCAAATGTTCCGCCTAATGTTGGTGTCCTTGATGAGCCTGGCACTGATGCTATGCCACTCATGCGCCCTAGAACGGTATGGACGGCAAGCGCGCACATCATCACAGCCGTCATCGGCTCCGGCGTGCTCTCCCTCGCCTGGTCGACTGCACAGCTGGGCTGGGTCGTGGGGCCGCTCACCCTGATGATCTTTGCCTTGATCACGTACTACACCTCTAGCCTTCTTGCTGACTGCTCCGCAGCGGCGATCAGCTCACCGGCAAGAGGAACTACACCTACATGGACGCTGTTGCCGCGTACCTGGGTCGATGGCAAGTCCTGTCCTGTGGTGTTTTCCAGTATGTTAACTTGGTTGGAACTGCCGTTGGGTATACAATTACAGCGTCCATCAGTGCAGCGGCCGTGCACAAGGCAAACTGCTTCCACAACAAGGGCCACGCGGCCGACTGCAGCACCTACGACACCATGTACATGGTCGTATTTGGGATCGTTCAGATCTTCTTCTCTCAGCTCCCTAACTTCAGCGACCTTTCGTGGCTGTCCATCGTCGCCGCCATCATGTCGTTCTCTTACTCCAGCATCGCCGTCGGCCTCTCGTTGGCGCGGACCATTTCAGGCCGTAGTGGTACGACCACTCTGACCGGCACTGAGATCGGAGTCGACGTTGATTCAGCCCAGAAGGTCTGGCTCGCGCTTCAAGCTCTTGGCAACATCGCGTTCGCTTACTCCTACTCCATGATTCTCATCGAAATCCAAGACACGGTGAAGTCTCCTCCAGCCGAGAACAAGACGATGAAGAAGGCGACGCTGATGGGCGTGACGACCACCACGGCGTTCTACATGCTTGCTGGCTGCCTCGGGTACTCGGCATTCGGGAACGCGGCGCCAGGGAACATCCTGACCGGGTTCGGCTTCTACGAGCCCTACTGGCTGATCGACTTCGCCAACGTCTGCATCGTGGTGCACCTGGTGGGCGCGTACCAGGTCTTCTCCCAGCCCATCTTCGCGGCCTTGGAGACGGCGGCCGCCAAGCGCTGGCCGAACGCCAGGTTCGTCACGCGCGAGCACCCCCTCGTGGCCGGCAGGTTCCACGTCAACCTGCTCAGGCTGACGTGGAGGACGGCGTTCGTGGTGGTGAGCACGGTGCTCGCCATCGTGTTGCCCTTCTTCAACGATATCCTGGGCTTCCTCG

GGCAGCGGCGTATACAGAAGTACACCAGCAGGTGGGTGGCGCTGCAGCTGCTCAGCTTCCTGTGCTTCCTGGTCTCGCTCGCCTCGGCGGTCGCGTCCATCGAGGGAGTCACCGAGTCGCTCAAACACTACGTTCCCTTTAAGACCAAGTCGTGA BRASSICA NAPUSSEQ ID NO: 81: AKE34780 AAP8 [Brassica napus] (protein)MKSLDTLHNPSAVESGNAAVKNVDDDGREKRTGTFLTASAHIITAVIGSGVLSLAWALAQLGWVAGTMILVIFAIITYYTSTLLADCYRAPDPITGTRNYTYMGVVRAYLGGKKVQLCGLAQYGNLVGVSIGYTITASISLVAIGKANCFHGKGHGAKCTASNYPYMVAFGGLQILLSQIPNFHKLSFLSIIAAVMSFSYASIGIGLAIAKVASGKVGKTTLTGTVIGVDVSASDKVWKAFQAVGDIAFSYAYTTILIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNIAPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKKW

LTVYFPVAMHIAQTKVKKYSGRWLALNLLVLVCLIVSALAAVGSIVGLINNVKKYKPFES IDSEQ ID NO: 82: AKE34780 AAP8 [Brassica napus] (genomic): Brara.F00660 | A06:3765107..3768058 forward

TTTTTTCAGTTTTATATAGAATTATGTTTCTGATCAACAATTTAGTCTACTGATATTAATAATTTTTGGTATTATATGGTAAAAATATTCTTGTGAAGATACATTTTTGATCTTTTCCTATTTTTTCATAAGATGGTCCCAGGAACAAATTAAGATCAAAGTAATGTTTTCTTGCAAGATCAAAGTAATCAACCATTTTTAGTGTATCCTATCTTTTGAGGAACATTATTTTGTGGTTCTAAATTTTTTTATTTTGAAAATTCTGCATGCTCTTCTTGGGAAGATATATGAGTTAATTATCAAAATCTACAAAAAGATAAAATAATTATGAAATTTATCTTCTTTCCAAAATTACTTAATGAATTGAATTGACTAGTGTAGGATTATCTCATTAAAGAATGCTATCATTAAATTTTGATTGTTGGCCTCCCAAAAAAAAATTGAATTCAAATGAGAGATTGATCCAAACTTATCCACAAAAACAAAAGATTATCCGACTTTTTAACATCAAAGGATTAATGACAATAAACAATTTCGATGCTTAAGTCCTGCTTCGTGTAATCGCTGCTGTTGATTGACAAAAACAAAGACTCCTATGTAATTTAGAAGAGTAACTAAGTTTTAGAATA

ATGTTTACATGAATCTTCAATGTTCGATCGAGCTAACGGTGGAAGTGGTATAATTA

TTCTATCGTTCTTTGAATATTTGCATTTACATAGTAGTCATATATATGATGTATTAGTTAGTGTATGGATCTATTAATGTTTTTTTTTTGCTCTTAGAAGTAATCTCTAACTACCGATTATGGATATATTAGTTAGAGAATATGGATCTACTACCACTTCTATTCTCTCTTTCACCAAAAAGGGATAAAGAAGAAGGTGGCATTTACCTTGAAGATAAGATGTTACTATCAACTAGAGTATTAGCCTAGTAGGCATGCATCTACAAAAAGGCTTGATGGATTTTTTAATTATATATGTGAAGCTCTAAAGATACTGAAGCTCAAATATGTTTTTATTTTTTTT

TCATTGACAAACAAACATGAGAACGTAGCACACATTTAAAAAGCAAAAACAGCTAATTATCACAACACACTTGTAATCTTCTTAAATATTCTTGTGTTATCCTCTGTTTTAGAAATTTAGATTAATAGTCGAAATTAGTAGAAATAGAGTTAGTTTGGTTTAAAATATAAT

SEQ ID NO: 83: BnaA01g21750D [Brassica napus] (protein)MKGFNTEQDHPAAESGNVYDVSDPTKNVDDDGREKRTGTWLTASAHIITAVIGSGVLSLAWWAIAQLGWIAGTLILVIFSFITYFTSTMLADCYRAPDPVTGKRNYTYMDVVRSYLGGRKVLCGVAQYGNLIGITVGYTITASISLVAVGKANCFHKKGHEADCTISNYPYMAVFGIIQIILSIPNFHKLSFLSLMAAVMSFTYATIGIGLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATVLIEIQASFHIKYLWNLVSFEYEPLDRIVDTLKSSPAENKSMKRASLVGVSTTTFFYILCGCLGYAAFGNKAPGDFLTDFGFYEPFWLIDFANACIAFHLIGAYQVFAQPIFQFVEKRCNRNWPDNKFITSEYSVNVPFLGKFNISLFRLVWR

CWVCLIVSLLAAAGSIAGLISSVKTYKPFRTIHESEQ ID NO: 84: BnaA01g21750D [Brassica napus] (genomic): >Brara.I01660 | A09:11198108..11202102 forward

GATTGAAAAATAGACACTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAAATGACAAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGTTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTTAAAAACAAATG

ATGTGATTTTACTTTATGACCGTTACCAAATGTACAGTTTAGTTATCTACCATATTG

AAACAACAACTACTAACAATTATTGGTCATCAAGTTGTAGTTTTCAAAGTACCTATATCAATAATTGTAACAAGATAGATAATACAATAAAGTAACGGTTGATATGTTACGATA

ATCAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGT

GTTTTCTTTTTGCACTTTCTCGCAGTTTAGCTAATGAAAAAGAAACATACTTCTTGC

SEQ ID NO: 85: BnaA06g38000D [Brassica napus] (protein)MKSFDAVHNPSAVESADANVDDDGREKRTGTLMTASAHIITAVIGSGVLSLAWAIAQLGWVAGTLILVTFAVVNYYTSTMLADCYRSDAGARNYTYMDVVRSYLGGRKVQLCGLAQYGCLVGVTIGYTITASISLVAIWKATCFHKKGHGAKCSIPNYPFMVAFGVVEIFLSQLPNFHKLSFLSIIAAIMSFSYASIGIGLAISVVASGKVGKTSVTGTVVGVDVTASDKIWKAFQATGDIAFSYSFSTILDTLRSNPPENKVMKKATLAGVSTTTVFYILCGCMGYAAFGNRAPGDFLTDFGFYEPYWLINFANACIVLHLIAAYQVFAQPIFQLVENKCNKAWPENNFIHKE

HISQRKVKKYSMKWNALKLLISVCLIVSLLAAIGSIVGLINSVKAYKPFHSSEQ ID NO: 86: BnaA06g38000D [Brassica napus] (genomic) Brara.F00658 A06:3747624..3750160 forward

GTTTGAAAATATAAACAACATGGTATAGTTCATTGGCTTTGAAAGATTTACTTTATTTTAGTTGTAAATAACTTAAGATTACTAAAATCGAATGAAGTTAGTTAGCATTAGTTTGATTTTGAAGATTTTTTTTTCGTCAAAAGTTGTAATCAATTTTTTTTATTTCTGTTTCG

TCTCCCTTTTTTTTGTCAAAAAAATATACATTTGTAAAACATGCTCACGCAATTTCAAAACCCTATGCAAATATCTTGACACATGCATTTAGTTGTTTAGTTTTCTGTTTTTACATAAAAATTTAGGAGCTCTGAGATACGTCTATACACATATTCAAAACTGATTATAAACTGGGTTGACTTGTTCGATTGCATTAGTTGTCAATCTTTTTAAATGCCCTCGTACATACAAAAAGTTTGTTTAGGCAGAAAAGCATCTCTATCTAAGCCTCATATATTGTAGGATTCTCACAGATTTGTAAGATGATATATGCATGTGATTTTTCTAACTAAAAAGGTGTGAT

TTCTTTTATAGATGTTTTTGGTGGTCATCATCAAAATAGCTAGTGTGGCAATTTTTTTATATCATTATTGCTTTTTATTTGCATTTGAGTTTAAAAATCATATGATATATGTTGGTTTGGTTAATAACTTTTGATGATGTTATTAATTATGATTCTTATCAAGAGAATATATATCTTGAATTTGACACAGATCACTTTAAATAATAATTAGCCTTCAACCGCGGTTCATAC

TAAACTCATAAGAATATAGCTTGAGTCTCAGATCAAGGATTGCTCGATTATAAAGAAATAATTAGTACCTACTACTAACAACTTTGAAGTTACCGAAGTCTCAAGATGAAAAC

SEQ ID NO: 87: BnaA06g38010D [Brassica napus] (protein)MKSFDTVESGDATGNNFDDDGREKRTGTLMTASAHIITAVVGSGVLSLAWAIAQLGWVAGIVILVTFAVINYYTSTMLADCYRSDTGTRNCTYMDVVRAYLGGKKVQLCGLAQYGCFVGVTIGYTITASISLVAIGKANCFHDKRHGAKCSMPNYPFMAVFGIVEIILSQIPSFHKLSFLSIIATVMSFSYASIGIGLAMAVVASGKVGKTGATGTVVGVDVTTSDKIWKSFQATGDIAFSYAYSSILDTLRSSPPENKVMKKASLAGVSTTTFFYMLCGCIGYAAFGNKAPGDFLTDFFYEPYWLIDFANACIVLHLIAAYQVFAQPIFQFVENKCNKAWPESNFITKEHS

SQRKIKKHSMRWIGLKLLVLVCLIVTLLAAIGSIVGLIKSVKAYKHFHSSEQ ID NO: 88: BnaA06g38010D [Brassica napus] (genomic) >Brara.F00658 | A06:3747624..3750160 forward

GTTTGAAAATATAAACAACATGGTATAGTTCATTGGCTTTGAAAGATTTACTTTATTTTAGTTGTAAATAACTTAAGATTACTAAAATCGAATGAAGTTAGTTAGCATTAGTTTGATTTTGAAGATTTTTTTTTCGTCAAAAGTTGTAATCAATTTTTTTTATTTCTGTTTCG

CTCTCCCTTTTTTTGTCAAAAAAATATACATTTGTAAAACATGCTCACGCAATTTCAAAACCCTATGCAAATATCTTGACACATGCATTTAGTTGTTTAGTTTTCTGTTTTTACATAAAAATTTAGGAGCTCTGAGATACGTCTATACACATATTCAAAACTGATTATAAACTGGGTTGACTTGTTCGATTGCATTAGTTGTCAATCTTTTTAAATGCCCTCGTACATACAAAAAGTTTGTTTAGGCAGAAAAGCATCTCTATCTAAGCCTCATATATTGTAGGATTCTCACAGATTTGTAAGATGATATATGCATGTGATTTTTCTAACTAAAAAGGTGTGA

TTCTTTTATAGATGTTTTGGTGGTCATCATCAAAATAGCTAGTGTGGCAATTTTTTTATATCATTATTGCTTTTTATTTGCATTTGAGTTTAAAAATCATATGATATATGTTGGTTTGGTTAATAACTTTTGATGATGTTATTAATTATGATTCTTATCAAGAGAATATATATCTTGAATTTGACACAGATCACTTTAAATAATAATTAGCCTTCAACCGCGGTTCATAC

SEQ ID NO: 89: BnaA09g57230D [Brassica napus] (protein)MKSYATEYNPSAVETAGNNFDDDGREKRTGTLMTATAHIITAVIGSGVLSLAWAIAQLGWVAGTVILVTFAVINYFTSTMLADCYRSPDTGIRNYNYMDVVRAYLGGWWKVKLCGLAQYGSLVGITIGYTITASISLVAIGKANCFHDKGHDAKCSVSNYPLMAAFGITQIVLSQIHNFHKLSFLSIIATVMSFSYASIGIGLALAALASGKVGKTDLTGTVVGVDVTASDKIWRSFQAAGDIAFSYAFSVVLVEIQACILSIRDDTLRSSPPENKVMKKASLAGVSTTTGFYILCGCIGYAAFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIAAYQVFAQPIFQFIEKKCNK

WPLTVYFPVEMHISQRKVKKYTMRWIGLKLLVLVCLVVSLLAAVGSIVGLISSVKAYKP FHNLDSEQ ID NO: 90: BnaA09g57230D [Brassica napus] (genomic)ATGAAAAGCTACGCCACTGAGTATAATCCCTCGGCCGTGGAAACCGCCGGGAATAACTTCGACGATGATGGTCGGGAGAAGAGAACGGGGACGTTGATGACGGCGACCGCGCACATAATCACGGCGGTGATAGGTTCTGGAGTCTTGTCGTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGCAGGAACGGTGATTTTGGTAACTTTTGCCGTTATAAATTACTTCACATCTACAATGCTTGCGGACTGCTATCGATCTCCGGACACAGGAATACGTAATTATAATTACATGGACGTTGTCAGAGCTTACCTTGGTGGTTGGAAAGTGAAGCTGTGTGGACTGGCACAGTACGGGAGTCTAGTAGGGATCACTATTGGCTACACCATCACTGCCTCCATAAGCTTAGTAGCGATCGGGAAAGCAAATTGTTTTCATGACAAGGGACATGATGCAAAATGTTCCGTATCAAATTATCCACTCATGGCGGCGTTTGGTATCACCCAGATTGTTCTTAGTCAGATTCATAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCTACCGTTATGTCCTTCTCTTATGCATCCATCGGAATTGGCTTAGCCTTGGCTGCTCTGGCAAGTGGGAAGGTTGGTAAGACGGATCTGACGGGCACGGTGGTTGGAGTAGACGTAACTGCGTCTGACAAAATATGGAGGTCGTTTCAAGCAGCTGGAGACATTGCCTTTTCGTACGCATTTTCCGTTGTTCTCGTTGAGATTCAGGCATGCATTCTTTCAATTAGAGATGATACACTGAGATCAAGCCCACCAGAGAACAAAGTCATGAAAAAAGCAAGCCTTGCTGGAGTTTCAACTACAACTGGTTTCTACATCTTGTGTGGCTGGTTTTTATGAGCCTTACTGGCTCATTGATTTTGCTAATGCTTGCATTGCTGTCCACCTAATCGCAGCCTATCAGGTGTTTGCACAACCAATATTCCAGTTTATTGAGAAGAAATGCAACAAAGCGTGGCCAGAAAGCAACTTCATCGCCAAAGATTATTCGATAAACATACCATTGCTAGGGAAATGTCGCATCAACTTCTTCAGATTGGTCTGGAGGTCAACCTATGTGATTTTGACAACAGTTGTAGCGATGATATTCCCCTTCTTTAACGCGATCTT

ATGCACATCTCGCAGAGAAAGGTTAAGAAGTATACTATGAGATGGATAGGGTTGAAACTCCTTGTATTGGTTTGTTTGGTTGTTTCGCTCCTAGCTGCGTAGGATCCATTGTCGGCTTGATAAGTAGTGTAAAGGCATACAAGCCTTTCCACAATTTAGATTAGSEQ ID NO: 91: BnA09g57240D [Brassica napus] (protein)MHRLYIDMSFTLHCLCFFSPLNMKTFDTSSAVESGTVAGNNVDDDGGEKRTGTLMTASAHIITAVIGSGVLSLAWAIAQLGWVAGTVLLVSFAVVVNYTSRMLADCYRSPDAGTRNNTYMDVVRAYLGGRKVQLCGLAQYGSLVGMTIGYTITASISLVAIGKANCFHDKGHGAKCLVSNYPAMAAFGIIQIVLSQIPNFHKLSFLSIIAAVMSFSYSSIGTGLALADLASGKVGKTELTGTVVGVDVTASDKLWKSFQAAGNIAFSYAYSVVLVEIQACIFSTRNDTLSSSPPENIVMKKASLVGVSTATAFYILCACMGYATFGSQAPGDLLTDFGFYEPYWLIDFANACIAVHLIGVYQQVIAQPIFQFVIKKCNKAWPESNFITLEHSMNIPLLGKCRINFFRLVWR

VCLIVSLLAAVGSIVGLISSLIRRKENMTLYISRLQFSHTHTHGPSTYPMINTNSYECLQNIISIDVCVHASSIYRYVIHSSSPMLLHISFLSSSVSPLKMKSFDTSSVVESGAGAGNNVDDDCREKRTGTLITASAHIITTVIGSGVLSLAWAIAQLGWVVGTVILVAFAVIVNYTSRMLADSYRSPEGTRNYTYMDVVRVYLGGRKVQLCGLAQFGSLVGVTIGYTITASISLVAIGKANCFHDKGHGAKCSVSNYPLMAAFGIVQIFLSQIPNFHKLSFLSIIATVMSFSYASIGFGLALAALASGKVGKTGLTGTVVGVDVTASDKLWKSFQAAGNIAFSYAYSVVLVEIQACIISINDDTLRSSPPENKVMKKASLAAVSTTTAFYILCGCIGYATFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIGAYQQVFAQPIFQFVEKKCNQAWPESNFITKEHSMNIPLLGKCRINFFRLVWRTTYVIFSTVVAMIFPFFNAILGLIGAVAFWPLTVYFPVEMHISQKKVKKYSVRWIVLKLLVLVCLIVSLLAAIGSIVSLISSVKAYKPFHNLDSEQ ID NO: 92: BnaA09g57240D [Brassica napus] (genomic): Brara.I05241 | A09:42950943..42954019 Brara.I05241 |A09:42950943..42954019 reverse

TGAACATATGTCGTTTTGTGACTCTATTACTTTGGTATTGTTTTAACCACAAAATAGTATATTTCCAAAAAGAGCTATATTTCTTTTTTTTCTTAAAAAAAATCATATACTTTCAACCCTAAAAAGGAATCTGAAACAAGTACAAAGGCCGATTCACCAGGTGGCTCTAGGTTACAAGGAGAGATTAACAAACAACAACAATGAACAGATTAACAAACAACAACAATGATATATATTTCCTTAGCTTAGTCTATTTTTTATTAAACAAAAACAAAAAACCATAAA

AACTTGTGATTTATTTTAATATATAACCTGTTCGTTTCACTTACGTGACCTATGACTAAATGTCGTTGCTCGTGTGCATATGTCGCATGATCTTGTGACCAGTTGCATGTATTACAGCGACATGCAAACGGCCATAATGTCACATGGTTACCAACACGTTAAGAGAATA

AAAAATATACGTGTAGAGCATTTTCAAGCTATTTCTAAACCCTATGCAAATATCTTGATACATACATCAATATTGTTTAATTTTCAGTAGAAATCATTCTCATTGATTTGTAAGATGATATATATTTATTACATATATGAATCTTCAAACTAATGACTAAAGCGGTATGATG

GAAATGTTGGTGGTCACACTCGGAATAGTGTAGCAATTTTTCCCTTTTGAAACACATTCTTTTATTTGCATTTATATTTTAATTACATGATATATATGTTGGTTGGAGTAATGACGGTTATTAGAGCACCATTAATCATAGTATTTTAGAAGGTTTATACTAATTAATTAAAATAAAAAGGAATATTGAAAAAAGGAGAAGAACAACAAATAGCAAAGATACTTCAAGAAAAAATTTGAGAAACTTTTCTATATGTGCAACTCATTTAGTAGTTGAGTTGTTTAAAAGTAATTAAAGTATACTTAATAAAAGTAAATATTAATATTTTATTTTTGTTGAGAAACGCTTTTTCCTTGTTGATGATGGTCTATGTATGTGTAAAACAAAACGTTATTGGGATTCTTATCATTTTTTTTTGACACAGAACTATATTATCTTCACTTAATTAAATACGTCTTCAACCGTCGTTTATGGTGGTTGTTTTCTGTTGCAATTCCCTTAAAGTATATATTGATGAAAGAGTTAATATGACGTATGCTAGCCCTTAATTAATTAATGACAGTATTGCTCAT

AAGATAGATAATTAGTATCTATCACTCACAATTTTTAAGTTTAAGTATAAGGCTCAA

SEQ ID NO: 93: BnaC01g42990D [Brassica napus] (protein)MKGFNTEQDHPAAESGNVYDVSDPTKNVDDDGREKRTGTWLTASAHIITAVIGSGVLSLAWAIAQLGWIAGTLILVIFSFITYFTSTMLADCYRAPDPVTGKRNYTYMDVVRSYLGGRKVQLCGVAQYGNLIGITVGYTITASISLVAVGKANCFHKKGHEADCTISNYPYMAVFGIIQIILSQIPNFHKLSFLSLMAAVMSFTYATIGIGLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATVLIEIQASFHIKYLWNLVSFEYEPLDRIVDTLKSSPAENKSMKRASLVGVSTTTFFYILCGCLGYAAFGNKAPGDFLTDFGFYEPFWLIDFANACIAFHLIGAYQVFAQPIFQFVEKCRNRNWPDNKFITSEYSVNVLFLGKFNISLFRLVWR

CWVCLIVSLLAAAGSIAGLISSVKTYKPFRTIHESEQ ID NO: 94: BnaC01g42990D [Brassica napus] (genomic) Brara.I01660 |A09:11198108..11202102 forward

GATTGAAAAATAGACACTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAAATGACAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGITTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTTAAAAACAAATG

ATGTGATTTTACTTTATGACCGTTACCAAATGTACAGTTTAGTTATCTACCATATTG

ATCAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGT

SEQ ID NO: 95: BnaC05g07760D [Brassica napus] (protein)YGNLVGVSIGYTITASISLVAIGKANCFHGKGHGAKCTASNYPYMGAFGGLQILLSQIPNFHKLSFLSIIAAVMSFSYASIGIGLAIAKVASGKVGKTTLTGTVIGVDVSASDKVWKAFQAVGDIAFSYAYTTILIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNIAPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKKWPESNFI

VAMHIAQTKVKKYSGRWLALHLLVLVCLIVSALAAVGSIVGLINNVKKYKPFESIDSEQ ID NO: 96: BnaC05g07760D [Brassica napus] (genomic)TACGGCAACCTCGTTGGGGTCTCTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATTGGGAAAGCAAATTGTTTTCATGGTAAGGGACATGGTGCGAAATGTACCGCATCGAATTATCCATACATGGGGGCATTTGGCGGCCTCCAGATTCTTCTAAGTCAGATTCCTAATTTTCACAAGCTATCTTTCCTCTCAATCATTGCCGCGGTTATGTCCTTCTCTTATGCATCTATTGGTATCGGTCTGGCCATCGCCAAAGTGGCAAGTGGGAAGGTTGGTAAGACAACGCTGACAGGTACGGTGATAGGAGTGGACGTATCTGCGTCTGATAAAGTATGGAAAGCGTTTCAAGCGGTTGGGGATATTGCGTTTTCGTACGCTTACACCACTATTCTCATTGAGATCCAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAGAAAGCAAGTCTTATTGGAGTCTCAACCACAACTGTTTTCTACCTCTTATGTGGTTGCATTGGGTATGCTGCATTCGGAAACATAGCCCCTGGTGACTTCCTTACCGACTTTGGGTTTTACGAACCTTTCTGGCTCGTCATTTTCGCCAATGTTTGCATTGCTGTCCATTTAGTAGGTGCCTATCAGGTATATGTTCAGCCCTTTTTCCAATTTGTTGAGAGCAAATGCAACAAAAAGTGGCCTGAAAGCAATTTCATCAACAAAGAGTACTCGTTGAAGATACCATTGCTCGGAAAATTTCGTGTCAACCACTTCAGGCTGGTGTGGAGGACAAACTATGTGATTTTGACAACATTTATTGCAATGATATTCCCCTTCTTCAACTCCATCTT

TGCACATTGCTCAGACAAAGGTTAAGAAGTATTCGGGTAGATGGTTGGCGCTGCACCTCCTCGTGTTGGTTTGCTTGATTGTCTCCGCCTTAGCTGCAGTGGGATCCATTGTTGGCCTAATCAATAATGTCAAGAAATACAAGCCTTTCGAGAGTATAGACTAASEQ ID NO: 97: BnaC05g49200D [Brassica napus] (protein)MKSFDAVHNPSAVESADANVDDDGREKRTGTLMTASAHIITAVIGSGVLSLAWAIAQLGWVAGTLILVTFAIVNYYTSTMLADCYRSDAGARNYTYMDVVRSYLGGRKVQLCGLAQYGCLVGITIGYTITASISLVAIWKATCFHKKGHGAKCSIPNYPFMAAFGVVEIFLSQLPNFHKLSFLSIIAAVMSFSYASIGIGLAIAVVASGKVGKTGVTGTVVGVDVTASDKIWKAFQATGDIAFSYSFSTILVEIQDTLRSSPPENKVMKKATLAGVSTTTVFYILCGCMGYAAFGNRAPGDFLTDFGFYEPYWLINFANACIVLHLIAAYQVFAQPIFQLVENKCNKAWPEN

FPVEMHISQRKKEFMYGPNPNFKGSRTPTPSIQQRGDTGSGNSGAAVMITVLDQFSEQ ID NO: 98: BnaC05g49200D [Brassica napus] (protein)ATGAAAAGCTTTGACGCGGTGCATAATCCCTCTGCGGTGGAATCCGCTGACGCCAACGTCGACGATGATGGTCGGGAGAAGAGAACGGGGACGTTGATGACGGCGAGTGCGCACATAATCACGGCGGTGATAGGTTCCGGAGTGTTGTCGTTGGCCTGGGCTATAGCACAGCTTGGTTGGGTGGCAGGAACACTGATTCTTGTAACTTTTGCCATCGTCAATTACTACACATCCACTATGCTCGCCGACTGTTATAGATCGGACGCAGGAGCTCGCAACTATACGTACATGGACGTCGTCCGATCTTACCTTGGTGGTAGGAAAGTGCAGTTATGTGGACTGGCACAATACGGGTGTCTCGTAGGGATCACTATTGGTTACACCATCACTGCCTCTATAAGTTTAGTAGCGATTTGGAAAGCAACTTGTTTTCATAAAAAAGGACATGGTGCGAAATGTTCCATCCCAAATTATCCATTCATGGCGGCCTTCGGGGTCGTGGAGATTTTTCTTAGTCAGCTTCCTAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCCGCCGTTATGTCATTCTCTTATGCGTCTATCGGAATTGGTTTAGCCATTGCCGTTGTGGCAAGTGGAAAGGTTGGTAAGACGGGTGTGACGGGCACGGTGGTTGGAGTGGACGTGACCGCATCTGACAAAATATGGAAGGCGTTTCAAGCAACTGGAGACATTGCATTTTCATACTCTTTTTCCACTATTCTCGTTGAGATTCAGGATACATTGAGATCAAGCCCACCAGAAAACAAAGTCATGAAAAAAGCAACACTCGCCGGAGTCTCAACGACAACTGTTTTCTACATCTTATGTGGCTGCATGGGATATGCTGCATTTGGAAACCGAGCCCCCGGAGACTTCCTTACTGACTTTGGTTTTTATGAACCTTACTGGCTCATCAACTTTGCCAATGCTTGCATCGTCCTCCACCTAATCGCAGCCTATCAGGTGTTTGCACAACCAATTTTCCAACTTGTTGAGAACAAATGCAACAAAGCATGGCCAGAAAACAATTTCATCAACAAAGAACATTCGATAAACATACCATTCCTCGGAAAATGGCGCATCAACTTCTTCAGACTGGTGTGGAGGACAGCATATGTGATTTTGACAACATTTGTTGCAGTGATATTCC

GTTTACTTCCCAGTGGAGATGCACATCTCGCAGAGAAAGAAGGAGTTCATGTATGGTCCAAATCCTAACTTCAAAGGCTCTAGAACTCCAACACCGTCTATTCAACAACGAGGAGACACTGGGAGTGGCAACTCCGGTGCTGCTGTGATGATCACGGTTCTAGA TCAGTTTTGASEQ ID NO: 99: BnaC05g49210D [Brassica napus] (protein)MKSFDTVESGDATGNNFDDDGREKRTGTLVTASAHIITAVVGSGVLSLAWAIAQLGWVAGIVILVTFAVINYYTSTMLADCYRSDTGTRNCTYMDVVRAYLGGRKVQLCGLAQYGCFVGVTIGYTITASISLVAIGKANCFHDKGHGAKCSMPNYPFMAAFGIVEIILSQIPSFHKLSFLSIIATVMSFSYASIGIGLAMAVVASGKVGKTGVTGTVAGVDVTASDKIWKSFQATGDIAFSYAYSSILVEIQACILSSIDVLGVIIKIDTLRSSPPENKVMKKASLAGVSTTTFFYMLCGCIGYAAFGNKAPGDFLTEFFYEPYWLIDYANACIVLHLIAAYQVFAQPIFQFVENKCNKAWPESNFITIEHSMNIPFLGKCRVNFFRLVWRTAYVILTTVVAMIFPFFNSILGLIGA

HFHSSEQ ID NO: 100: BnaC05g4910D [Brassica napus] (genomic) Brara.F00658 |A06:3747624..3750160 forward

GTTTGAAAATATAAACAACATGGTATAGTTCATTGGCTTTGAAAGATTTACTTTATTTTAGTTGTAAATAACTTAAGATTACTAAAATCGAATGAAGTTAGTTAGCATTAGTTTGATTTTGAAGATTTTTTTTTCGTCAAAAGTTGTAATCAATTTTTTTTATTTCTGTTTCG

CTCTCCCTTTTTTTTGTCAAAAAAATATACATTTGTAAAACATGCTCACGCAATTTCAAAACCCTATGCAAATATCTTGACACATGCATTTAGTTGTTTAGTTTTCTGTTTTTACATAAAAATTTAGGAGCTCTGAGATACGTCTATACACATATTCAAAACTGATTATAAACTGGGTTGACTTGTTCGATTGCATTAGTTGTCAATCTTTTTAAATGCCCTCGTACATACAAAAAGTTTGTTTAGGCAGAAAAGCATCTCTATCTAAGCCTCATATATTGTAGGATTCTCACAGATTTGTAAGATGATATATGCATGTGATTTTTCTAACTAAAAAGGTGTGA

TTCTTTTATAGATGTTTTGGTGGTCATCATCAAAATAGCTAGTGTGGCAATTTTTTTATATCATTATTGCTTTTTATTTGCATTTGAGTTTAAAAATCATATGATATATGTTGGTTTGGTTAATAACTTTTGATGATGTTATTAATTATGATTCTTATCAAGAGAATATATATCTTGAATTTGACACAGATCACTTTAAATAATAATTAGCCTTCAACCGCGGTTCATAC

TAAACTCATAAGAATATAGCTTGAGTCTCAGATCAAGGATTGCTCGATTATAAAGAAATAATTAGTACCTACTACTAACAACTTTGAAGTTACCGAAGTCTCAAGATGAAAAC

SEQ ID NO: 101: BnaC08g42410D [Brassica napus] (protein)MKSFDTSSVVESGAGAGNNVDDDCREKRTGTLITASAHIITTVIGSGVLSLAWAIAQLGWVVGTVILVAFAVIVNYTSRMLADSYRSPEGTRNYTYMDVVRVYLGGRKVQLCGLAQFGSLVGVTIGYTITASISLVAIGKANCFHDKGHGAKCSVSNYPLMAAFGIVQIFLSQIPNFHKLSFLSIIATVMSFSYASIGFGLALAALASGKVGKTGLTGTVVGVDVTASDKLWKSFQAAGNIAFSYAYSVVLVEIQACILSINDDTLRSSPPENKVMKKASLAAVSTTTAFYILCGCIGYATFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIGAYQQVFAQPIFQFVEKKCNQAWPESNFITKEHSMNVPLLGKCRINFFRLVWRTTYVIFSTVVAMFPFFNAILGLIGA

PFHNLDSEQ ID NO: 102: BnaC08g42410D [Brassica napus] (genomic): Brara.I05240 |A09.42945936..42949113 reverse

TGATATATAAAACATGTTTGTTTCAATTTCTAACGTGACCTACGACTAAGTATTGCTCACATGGCCATAATGTCATATGGTTACCAATATGTTAAGAAAATATTTAAGTCTGGT

ACATTCTCAAGCTATTTCTAAACCCTATGCAAATATCTTGATACATACTTAATACATTTATATTTTAGTTTTCAGTACAAATCTTTCTTTTTCAGTAGAAATCATCCTCATTGATTTGTAAGATGATATATATTAACTATTTACACATCTATTGTTTAAAAAAAAAAAATTTAT

CTCAGAAATCAGAATAGTGTAGCATTTTCTAATATTACAGTGAAACTTCTATAAATTAATAATGTTGGGACTACATCAAAACTATAATTTTTTTATTAATTTATAGAGATACTAATTTATCGATATACTAATAGAACCAAAAACTCAATTTGAAACTATAAAATTATATTATTTTATAGATTTTTAGTATATATTAATTTATAGATTATTAATTTAAAGAGGTTATACTGTAGTTTTTTTATTCTTTTATTTACATGATATATATTTTGGTTGGAATAATGACTGTTATTAGAGCACCATTAGTCATAGTATTAGTATCGTAGGGGGTGTCTAATAATTAAAATAAAAAGGAATATTGAAAAAGAGAAGAACAGAAAATAGCAAAAACGATTCTTGTTGACATACTTCAAGAAAAAAAAGTCCGATTTTTTTACAAGTGTAACTCATTTATTAGTTGGGTTGTTTAAAAGTAATTAAAGTATACTTGTAAAACTAAATATTTTTGGCACCGAACTATACTATTATTTTCACTACAATACGTCTTCAACCGTCGTTTATGGTGGTTGTTTTCTTGTTGTAATTCCCTTAAAGTATATATTGATGGATGATTAGAGTTAATATGATGTATGTT

ACTCATAAAAAAGGAGAAATACTTTAGAATATTACTAAAAACAGCTTACTATTCTAAATTAACACACGCAAAATGATCAAAATAACATTAACTAAAATTTAAAAATATACTTTTATTTTATAGTTGGGTTTAGGTTTAGTGAATAGAGTTTAGGGGTTAGTATTTAAAAAGTGGAAGTGCAGAGTTTGAAATGTTTTTTGTCATTTTCTCCTTATGTGATAATTTTGTCATAATATTTTTTTGTGGTATCTAAGTCATTTGTCCTAAAAAAGTCAAGAGTTTAATAT

SEQ ID NO: 103: BnaC08g42420D [Brassica napus] (protein)MHRLYIDMSFTLHCLCFFSPLNMKSFDTSSAVESGTVAGNNVDDDGGEKRTGTLMTASAHIITAVIGSGVLSLAWAIAQLGWVAGTVLLVSFAVVVNYTSRMLADCYRSPDAGTRNNTYMDVVRAYLGGRKVQLCGLQAYGSLVGMTIGYTITASISFVAIGKANCFHDKGHGAKFSVSNYPAMAAGFIIQIVLSQIPNFHKLSFLSIIAAVMSFSYSSIGTGLALADLASGKVGKTELTGTVVGVDVTASDKLWKSFQAAGNIAFSYAYSVVLVEIQACIFSTRNDTLSSSPPENIVMKKASIVGVSTATAFYILCACMGYATFGSQAPGDLLTDFGFYEPYWLIDFANACIAVHLIGAYQQVIAQPIFQFVEKKCNKAWPESNFITKEHSMNIPLLGKCRINFFRLVWRT

CLIVSLLAAVGSIVGLISSVKAYKPFHNLDSEQ ID NO: 104: BnaC08g4240D [Brassica napus] (genomic): Brara.I05241 |A09:42940943..42954019 reverse

TGAACATATGTCGTTTTGTGACTCTATTACTTTGGTATTGTTTTAACCACAAAATAGTATATTTCCAAAAAGAGCTATATTTCTTTTTTTTCTTAAAAAAAATCATATACTTTCAACCCTAAAAAGGAATCTGAAACAAGTACAAAGGCCGATTCACCAGGTGGCTCTAGGTTACAAGGAGAGATTAACAAACAACAACAATGAACAGATTAACAAACAACAACAATGATATATATTTCCTTAGCTTAGTCTATTTTTTATTAAACAAAAACAAAAAACCATAAA

AAAAATATACGTGTAGAGCATTTTCAAGCTATTTCTAAACCCTATGCAAATATCTTGATACATACATCAATATTGTTTAATTTTCAGTAGAAATCATTCTCATTGATTTGTAAGATGATATATATTTATTACATATATGAATCTTCAAACTAATGACTAAAGCGGTATGATG

GAAATGTTGGTGGTCACACTCGGAATAGTGTAGCAATTTTTCCCTTTTGAAACACATTCTTTTATTTGCATTTATATTTTAATTACATGATATATATGTTGGTTGGAGTAATGACGGTTATTAGAGCACCATTAATCATAGTATTTTAGAAGGTTTATACTAATTAATTAAAATAAAAAGGAATATTGAAAAAAGGAGAAGAACAACAAATAGCAAAGATACTTCAAGAAAAAATTTGAGAAACTTTTCTATATGTGCAACTCATTTAGTAGTTGAGTTGTTTAAAAGTAATTAAAGTATACTTAATAAAAGTAAATATTAATATTTTATTTTTGTTGAGAAACGCTTTTTCCTTGTTGATGATGGTCTATGTATGGTGTAAAACAAAACGTTATTGGGATTCTTATCATTTTTTTTTGACACAGAACTATATTATCTTCACTTAATTAAATACGTCTTCAACCGTCGTTTATGGTGGTTGTTTTCTGTTGCAATTCCCTTAAAGTATATATTGATGAAAGAGTTAATATGACGTATGCTAGCCCTTAATTAATTAATGACAGTATTGCTCAT

TAAGATAGATAATTAGTATCTATCACTCACAATTTTTAAGTTTAAGTATAAGGCTCA

SEQ ID NO: 105: BnaC08g42430D [Brassica napus] (protein)MKSFHTEYNPSAVEAAGNNFDDDGREKRTGTVMTASAHIITAVIGSGVLSLAWAIAQLGWVAGTVILVTFAVINYFTSTMLADCYRSPDTGIRNYNYMDVVRAYLGGWKVKLCGLAQYGSLVGITIGYTITASISLVAIGKANCFHEKGHGAKCSVSNYPLMAAFGIIQIVLSQIHNFHKLSFLSIIATVMSFSYASIGIGLALAALASGKVGKTDLTGTVVGVDVTASDKIWRSFQAAGDIAFSYAFSVVLVEIQACILSIRDDTLRSSPPENKVMKKASLAGVSTTTGFYILCGCIGYAAFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIAAYQVFAQPIFQFIEKKCNK

PLTVFPVEMHISQKKVKKYTMRWIGLKLLVLVCLVVSLLAAVGSIVGLISSVKAYKPFH NLDSEQ ID NO: 106: BnaC08g42430D [Brassica napus] (genomic)ATGAAAAGCTTCCACACTGAGTATAATCCCTCGGCCGTGGAAGCCGCCGGGAATAACTTCGACGACGATGGTCGGGAGAAGAGAACGGGGACGGTGATGACGGCAAGTGCTCACATTATCACTGCTGTGATAGGTTCCGGAGTCTTGTCCTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGCAGGAACAGTGATTTTGGTAACTTTTGCCGTTATAAATTACTTCACATCTACAATGCTTGCCGACTGTTATCGATCTCCGGACACAGGAATACGTAATTATAATTACATGGACGTTGTCAGAGCTTACCTTGGTGGTTGGAAAGTGAAGCTATGTGGTCTGGCACAGTACGGGAGTCTAGTAGGGATCACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATAGGGAAAGCAAATTGTTTTCATGAAAAGGGACATGGTGCAAAATGTTCCGTATCGAATTATCCACTCATGGCGGCGTTTGGTATCATCCAGATTGTTCTTAGTCAGATTCATAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCCACCGTTATGTCCTTCTCTTATGCATCCATCGGAATTGGCTTGGCCTTGGCCGCTCTGGCAAGTGGGAAGGTTGGTAAGACGGATCTGACGGGCACGGTGGTTGGAGTAGACGTAACTGCGTCTGACAAAATATGGAGGTCGTTTCAAGCAGCTGGAGACATTGCCTTTTCGTACGCATTTTCCGTTGTTCTCGTTGAGATTCAGGCATGCATTCTTTCAATTAGAGATGATACACTGAGATCAAGCCCACCAGAGAACAAAGTCATGAAAAAAGCAAGCCTTGCTGGAGTTTCAACTACAACTGGTTTCTACATCTTATGTGGCTGCATCGGATATGCTGCTTTTGGAAACCAAGCCCCTGGAGACTTCCTAACTGACTTTGGTTTTTATGAGCCTTACTGGCTCATTGATTTTGCTAATGCTTGCATTGCTGTCCACCTAATCGCAGCCTATCAGGTGTTTGCACAACCAATATTCCAGTTTATTGAGAAGAAATGCAACAAAGCGTGGCCAGAAAGCAACTTTATCACCAAAGATTATTCGATAAACATACCATTGCTAGGGAAATGTCGCATCAACTTCTTCAGATTGGTCTGGAGGTCAACCTATGTGATTTTGACAACAGTTGCAGCAATGATATTCCCCTTCTTCAACGCGAT

GAGATGCACATCTCGCAGAAAAAGGTTAAGAAGTATACTATGAGATGGATAGGGTTGAAACTCCTTGTATTGGTTTGTTTGGTTGTTTCGCTCCTAGCTGCAGTAGGATCCATTGTCGGCCTCATAAGTAGTGTAAAGGCATACAAGCCTTTCCACAATTTAGATTA GSEQ ID NO: 107: BnaCnng14480D [Brassica napus] (protein)MEKKSMFIEQSFTDHKSGDMNKNFDDDGRQKRTGTWMTGSAHIITAVIGSGVLSLAWAIAQLGWVAGPAVLMAFSFITYFTSTMLADCYRSPDPVTGKRNYTYMEVVRSYLGGRKVMLCGLAQYGNLIGITIGYTITASISMVAVKRSNCFHKNGHNVKCSTSNTPFMIIFACIQIVLSQIPNFHNLSWLSILAAVMSFSYASIGVGLSIAKVAGGGVHARTALTGVTVGVDVTGSDKVWRTFQAVGDIAFAYAYSTDTLKASPPSENKAMKRASLVGVSTTTFFYMLCGCVGYAAFGNNAPGNFLTGFGFYEPFWLIDFANVCIAVHLVGAYQVFCQPIFQFVSQSAKRWPDNKFITGEYKMNVPCGGDFGISLFRLVWRTSYVVVTAVVAMIFPFFNDFLGLI

LLNADLFTKSVAPESSEQ ID NO: 108: BnaCnng14480D [Brassica napus] (genomic): Brara.B01675 |A02:9628389.9631130 reverse

TTCTTTCAGAATGATAGTCAATAATAGCAGGCTCTCCTTTTTCACCTATTTTCACCCACGTTTATTATGTTAGGACAGGTGACTAACTCTTTTTTATAATTATTAATTTTACCTTTTAAAGAACAGATGCTATGATAGGTAAGAGATATGATATATAATATCTACAAAAGTTTTTCTTTGTCACAAGTTATTTGATATGTACAGAGTAATATAAATTTAAATTCTATTGAGTGTGGGAGTCGAAAGGAGCTCAAATTTTCAAAGTGAAAAGTTAGATCTAGTAGGATCGTTGAGATTTTGTATTCTAAATTTATCAAATTTTTTTTGTCTGGAACTTTATATATTTATAATTATTAAGGAATGGGTTTTAAAGTACGAAAGAAAGAAAAAATTAAAATG

TCACCAGTTTAATTTAAAATGTTTTTTAAACATCTCGCACACCTGTTAAGAAAGGAGTATTAGTTTTTCACTATAACCCTTATTAAATGTTTCAGCTAATACAAATGGTATCTTTGGAAAAAATAATAATATACTCAGACCTGAATATACTACATATTTTTATAATTTAATATAACGGAAAATATGGTTATAATTGAAAGTTGAAACTTATGTTAAAACTTTGTATTGGCAACTCTAAAACTAAACTCAGTTTTAAAAAATTAGCCATAAACTGACACTTCTGAAATGGAAGGATTATGTTTAGAGCTGAGTTTTAGAGACCGCGATAAGAGAGAGCCAAAAAAAAATTAAACGTGTGTCGGTTTGTGACAAAGTAAAGGCCACTTCAGATGAATTATTATTTGTAGTAAAACATGAGAGGAAACCAGAGTCTAACTAGTAGGCTTTTATTCAATAAATAAATACTTATAAAATGATTTAACTTTGAGTACGGTTTACAACTGATGTTTTGGCTTCTTTTGTACACAAAAGATTAATATTCTAACTTTAATTAATGTATTTCATTTTAAAAGTAAAAAAAAATCTAAAAATATGGATTGTAGAATTTTATTGGAAAAAAACAAAACTAACAAAAACTAATTAATCAAAAATGACTCTTGACTTAGTTAATTTTTATACTTTTATATTTATCACTAAATAACATTAAAGTCACCAATTACGTATTGTCATTTCAGATAATTGTAAACGATTTAGTGAACTACATTTTGTGTGTGTTTTGATCTACCACTACTAAAGTATGT

SEQ ID NO: 109: BnaCnng25620D [Brassica napus] (protein)MKSFNTDQHGHSAAESADVYAMSDPTKNVDDDGREKRTGTWLTASAHIITAVIGSGVLSLAWAIAQLGWIAGTLILIIFSFITYFTSTMLADCYRAPDPLTGKRNYTYMDVVRSYLGGRKVQLCGVAQYGNLIGITVGYTITASISLVAIGKANCYHNKGHHADCTISNYPYMAAFGIIQILLSQIPNFHKLSFLSLMAAVMSFAYASIGIGLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATDTLRSSPAENKAMKRASFVGVSTTTFFYILCGCLGYAAFGNKAPGDFLTDFGFYEPFWLIDFANACIAFHLIGAYQVFAQPIFQFVEKKCNR

WPLTVYFPVEMHIAQTKVKKYSSRWIGLKMLCWVCLIVSLLAAAGSIAGLISSVKTYKP FRTIHESEQ ID NO: 110: BnaCnng25620D [Brassica napus] (genomic): ara.I01660 |A09:11198108..11202102 forward

GATTGAAAAATAGACACTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAAATGACAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGTTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTTAAAAACAAATG

ATCAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGT

SEQ ID NO: 111: CAD92449 AAP1 [Brassica napus] (protein)MKSFNTDQHGHSAAESGDVYAMSDPTKNVDDDGREKRTGTWLTASAHIITAVIGSGVLSLLAWAIAQLGWIAGTLILIIFSFITYFTSTMLADCYRAPDPLTGKRNYTYMDVVRSYLGGRKVQLCGVAQYGNLIGITVGYTITASISLVAIGKANCYHNKGHHADCTISNYPYMAAFGIIQILLSQIPNFHKLSFLSLMAAVMSFAYASIGIGLAIATVAGGKVGKTNMTGTVVGVDGIIQILLSQIPNFHKLSFLSLMAAVMSFAYASIGIGLLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATVLIEIQDTLRSSPAENKAMKRADFVGVSTTTFFYILCGCLYGYAAFGNKAPGDFLTNFGFYEPFWLIDFANACIAFHLIGAYQVFAQPIFQFVEKKCNRNWPDNKFITSEYSVNIPFLGKFSINLFRLVWRTAYVVITTLVAMIFPFFNAILGLIG

TYKPFRTIHESEQ ID NO: 112: CAD92449 AAP1 [Brassica napus] (genomic): Brara.I01660 |A09:11198108..11202102 forward

GATTGAAAAATAGACACTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAATGACAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGTTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTTAAAAACAAATG

ATCAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGT

SEQ ID NO: 113: XP_013645981 AAP8-like [Brassica napus] (protein)MKSLDTLHNPSAVEGNAAVKNDDDGREKRTGTFLTASAHIITAVIGSGVLSLAWALAQLGQVAGTMILVIFAIITYYTSTLLADCYRAPDPITRTRNYTYMGVVRAYLGGKKQLLCGLAQYGNVGVSIGYTITASISLVAIGKANCFHGKGHGAKCTASNYPYMGAFGGLQILLSQIPNFHKLSFLSIIAAVMSFSYASISISLLGLAIAKVASGKVGKTTLTGTVIGVDVSASDKVWKAFQAVGDIAFSYAYTTILLIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNLSPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKK

PLTVFPVAMHIAQTKVKKYSGRWLALNLLVLVCLIVSALAAVGSIVGLINNVKKYKPFE SIDSEQ ID NO: 114: XP_013645981 AAP8-like [Brassica napus] (genomic)ATGAAATCCTTGGACACACTCCACAATCCCTCGGCGGTTGAGTCCGGTAACGCCGCTGTGAAGAACGTCGACGATGATGGTCGAGAGAAGAGAACGGGGACGTTTCTGACGGCGAGTGCGCACATTATCACGGCGGTGATAGGCTCAGGAGTGTTGTCTTTGGCTTGGGCATTAGCACAGCTTGGTTGGGTGGCTGGAACCATGATTTTGGTGATTTTCGCCATCATCACTTACTACACCTCTACTTTGCTCGCCGATTGCTACAGAGCGCCGGACCCCATCACCAGAACACGCAACTACACGTACATGGGCGTCGTTCGAGCTTACCTTGGTGGTAAAAAGGTGCAGCTATGTGGACTAGCACAGTACGGCAACCTCGTTGGGGTCTCTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATTGGGAAAGCAAATTGTTTTCATGGTAAGGGACATGGTGCGAAATGTACCGCATCGAATTATCCATACATGGGGGCATTTGGCGGCCTCCAGATTCTTCTAAGTCAGATTCCTAATTTTCACAAGCTATCTTTCCTCTCAATCATTGCCGCGGTTATGTCCTTCTCTTATGCATCTATTGGTATCGGTCTGGCCATCGCCAAAGTGGCAAGTGGGAAGGTTGGTAAGACAACACTGACAGGTACGGTGATAGGAGTGGACGTATCTGCGTCTGATAAAGTGTGGAAAGCGTTTCAAGCGGTTGGGGATATTGCGTTTTCGTACGCTTACACCACTATTCTCATTGAGATACAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAGAAAGCAAGTCTTATTGGAGTCTCAACCACAACTGTTTTCTACCTCTTATGTGGTTGCATTGGGTATGCTGCATTCGGAAACTTATCCCCTGGTGACTTCCTTACCGACTTTGGGTTTTACGAACCTTTCTGGCTCGTCATTTTCGCCAATGTTTGCATTGCTGTCCATTTAGTAGGTGCCTATCAGGTATATGTTCAGCCTTTTTTCCAGTTTGTTGAGAGCAAATGTAACAAAAAGTGGCCTGAAAGCAATTTCATCAACAAAGAATACTCGTTGAAGATACCATTGCTCGGAAAATTTCGTGTCAACTTCTTCAGGCTGGTGTGGAGGACAAACTATGTGATTTTGACAACATTTATTGCAATGATATTCCCCTTCTTCAACTCCATCTTGGGTTT

ATTGCTCAGACAAAGGTTAAGAAGTATTCGGGTAGATGGTTGGCGCTGAACCTCCTCGTGCTGGTTTGCTTGATTGTCTCCGCCCTAGCTGCTGTGGGATCCATTGTTGGCCTAATCAATAATGTCAAGAAATACAAGCCTTTCGAGAGTATAGACTAASEQ ID NO: 115: XP_013661681 AAP1 X1[Brassica napus] (protein)MKSFNTDQHGHSAAESGDVYAMSDPTKNVDDDGREKRTGTWLTASAHIITAVIGSGVLSLAWAIAQLGWIAGTLIIIFSFITYFTSTMLADCYRAPDPLTGKRNYTYMDVVRSYGGRKVQLCGVAQYGNLIGITVGYTITASISLVAIGKANCYHNKGHHADCTISNYPYMAAFGIIQILLSQIPNFHKLLSFLSLMAAVMSFAYASIGIGLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATVLIEIQDTLRSSPAENKAMKRASFVGVSTTTFFYILCGCLGYAAFGNKAPGDFLTDFGFYEPFWLIDFANACIAFHLIGAYQVKPNPKGEKDCFLFALSRSLANEKETYFLQVFAQPIFQFVEKKCNRNWPDNKFITSEYSVNIPFLGKFNIN

WIGLKMLCWVCLIVSLLAAAGSIAGLISSVKTYKPFRTIHESEQ ID NO: 116: XP_013661681 AAP1 X1[Brassica napus] (genomic)

GATTGAAAAATAGACACTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAAATGACAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGITTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTTAAAAACAAATG

ATGTGATTTTACTTTATGACCGTTACCAAATGTACAGTTTAGTTATCTACCATATTG

AAACAACAACTACTAACAATTATTGGTCATCAAGTTGTAGTTTTCAAAGTACCTATATCAATAATTGTAACAAGATAGATAATACAATAAAGTAACGGTTGATATGTTACGATA

ATCAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGT

GTTTTCTTTTTGCACTTTCTCGCAGTTTAGCTAATGAAAAAGAAACATACTTCTTGC

SEQ ID NO: 117: XP_013661682 AAP1 X2 [Brassica napus] (protein)MKSFNTDQHGHSAAESGDVYAMSDPTKNVDDDGREKRTGTWLTASAHIITAVIGSGVLSLAWAIAQLGWIAGTLILIIFSFITYFTSTMLADCYRAPDPLTGKRNYTYMDVVRSYLGGRKVQLCGVAQYGNLIGITVGYTITASISLVAIGKANCYHNKGHHADCTISNYPYMAAFGIIQILLSQIPNFHKLSFLSLMAAVMSFAYASIGIGLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATVLIEIQDTLRSSPAENKAMKRASFVGVSTTTFFYILCGCLGYAAFGNKAPGDFLTDFGFYEPFWLIDFANACIAFHLIGAYQVFAQPIFQFVEKKCNRNWPDNKFITSEYSVNIPFLGKFNINLFRLVWRTAYVVITTLVAMIFPFFNAILGLIG

TYKPFRTIHESEQ ID NO: 118: XP_013661682 AAP1 X2 [Brassica napus] (genomic)

GATTGAAAAATAGACACTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAAATGACAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGTTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTAAAAACAAATGTAA

CAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGGA

SEQ ID NO: 119: XP_013661683 AAP1 X3[Brassica napus] (protein)MKSFNTDQHGHSAAESGDVYAMSDPTKNVDDDGREKRTGTWWLTASAHIITAVIGSGVLSLAWAIAQLGWIAGTLLILIIFSFITYFTSTMLADCYRAPDPLTGKRNYTYMDVVRSYLGGRKVQLCGVAQYGNLIGITVGYTITASISLVAIGKANCYHNKGHHADCTISNYPYMAAFGIIQILLSQIPNFHKLSFLSLMAAVMSFAYASIGIGLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATVLIEIQDTLRSSPAENKAMKRASFVGVSTTTFFYILCGCLGYAAFGNKAPGDFLTDFGFYEPFWLLIDFANACIAFHLIGAYQVFAQPIFQFVEKKCNRNWPDNKFITSEYSVNIPFLLGKFNINLFRLVWRTAYVVITTLVAMIFPFFNAILGLIG

TYKPFRTIHESEQ ID NO: 120: XP_013661683 AAP1 X3[Brassica napus] (genomic):Brara.I01660 | A09:11198108..11202102 forward

GATTGAAAAATAGACACTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAAATGACAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGTTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTTAAAAACAAATG

AAACAACAACTACTAACAATTATTGGTCATCAAGTTGTAGTTTTCAAAGTACCTATATCAATAATTGTAACAAGATAGATAATACAATAAAGTAACGGTTGATATGTTACGATA

ATCAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGT

SEQ ID NO: 121: XP_013676681 AAP6 [Brassica napus] (protein)MEKKSMFIEQSFTDHKSGDMNKNFDDDGRQKRTGTWMTGSAHIITAVIGSGVLSLAWAIAQLGWVAGPAVLMAFSFITYFTSTMLADCYRSPDPVTGKRNYTYMEVVRSYLLGGRKVMLCGLLAQYGNLIGITIGYTITASISMVAVKRSNCFHKNGHNVKCSTSNTPFMIIFACIQIVLSQIPNFHNLSWLSILAAVMSFSYASIGIGLSIAKVAGGGVHARTALTGVTVGVDVTGSDKVWRTFQAVGDIAFAYAYSTVLIEIQDTLKASPPSENKAMKRASLVFVSTTTFFYMLCGCVGYAAFGNNAPGNFLTGFGFYEPFWLIDFANCIAVHLVGAYQVFCQPIFQFVESQSAKRWPDNKFITGEYKMNVPCGGDFGISLFRLVWRTSYVVVTAVVAMIFPFFND

GLIQSLKDFKPFQAPESEQ ID NO: 122: XP_013676681 AAP6 [Brassica napus] (genomic): Brara.B01675| A02:9628389..9631130 reverse

TTCTTTCAGAATGATAGTCAATAATAGCAGGCTCTCCTTTTTCACCTATTTTCACCCACGTTTATTATGTTAGGACAGGTGACTAACTCTTTTTTATAATTATTAATTTTACCTTTTAAAGAACAGATGCTATGATAGGTAAGAGATATGATATATAATATCTACAAAAGTTTTTCTTTGTCACAAGTTATTTGATATGTACAGAGTAATATAAATTTAAATTCTATTGAGTGTGGGAGTCGAAAGGAGCTCAAATTTTCAAAGTGAAAAGTTAGATCTAGTAGGATCGTTGAGATTTTGTATTCTAAATTTATCAAATTTTTTTTGTCTGGAACTTTATATATTTATAATTATTAAGGAATGGGTTTTAAAGTACGAAAGAAAGAAAAAATTAAATG

TCACCAGTTTAATTTAAAATGTTTTTTAAACATCTCGCACACCTGTTAAGAAAGGAGTATTAGTTTTTCACTATAACCCTTATTAAATGTTTCAGCTAATACAAATGGTATCTTTGGAAAAAATAATAATATACTCAGACCTGAATATACTACATATTTTTATAATTTAATATAACGGAAAATATGGTTATAATTGAAAGTTGAAACTTATGTTAAAACTTTGTATTGGCAACTCTAAAACTAAACTCAGTTTTAAAAAATTAGCCATAAACTGACACTTCTGAAATGGAAGGATTATGTTTAGAGCTGAGTTTTAGAGACCGCGATAAGAGAGAGCCAAAAAAAAATTAAACGTGTGTCGGTTTGTGACAAAGTAAAGGCCACTTCAGATGAATTATTATTTGTAGTAAAACATGAGAGGAAACCAGAGTCTAACTAGTAGGCTTTTATTCAATAAATAAATACTTATAAAATGATTTAACTTTGAGTACGGTTTACAACTGATGTTTTGGCTTCTTTTGTACACAAAAGATTAATATTCTAACTTTAATTAATGTATTTCATTTTAAAAGTAAAAAAAAATCTAAAAATATGGATTGTAGAATTTTATTGGAAAAAAACAAAACTAACAAAAACTAATTAATCAAAAATGACTCTTGACTTAGTTAATTTTATACTTTTATATTTATCACTAAATAACATTAAAGTCACCAATTACGTATTGTCATTTCAGATAATTGTAAACGATTTAGTGAACTACATTTTGTGTGTGTTTTGATCTACCACTACTAAAGTATGT

SEQ ID NO: 123: XP_013696427; XP_013640943; XP_013716098; AAP8[Brassica napus] (protein)MSPSSPPPTMKSLKTLHNPSAVESGNAAVKNVDDDGREKRTGTFLTASAHIITAVIGSGVLSLAWALAQLGWVAGTMILVIFAIITYYTSTLLLADCYRAPDPITRTRNYTYMGVVRAYLGGKKVQLCGLAQYGNLVGVSIGYTITASISLVAIGKANCFHGKGHGAKCTASNYPYMGAFGGLQILLSQIPNFHKLSFLSIIAAVMSFSYASIGIGLAIAKVASGKVGKTTLTGTVIGVDVSASDKVWKVFQAVGDIAFSYAYTTILIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNIAPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKKWPESNFINKEYSLKIPLLGKFRVNHFRLVWRTNYVILTTFIAMIFPFFNSILGLLG

YKPFESID SEQ ID NO: 124: XP_013696427; XP_013640943; XP_013716098; AAP8[Brassica napus] (genomic)ATGTCTCCCTCTCCCCCTCCTACAATGAAATCCTTGGACACACTCCACAATCCCTCGGCGGTTGAGTCCGGTAACGCCGCTGTGAAGAACGTCGACGATGATGGTCGAGAGAAGAGAACGGGGACGTTTCTGACGGCGAGTGCGCACATTATCACGGCGGTGATAGGCTCAGGAGTGTTGTCTTTGGCTTGGGCATTAGCACAGCTTGGTTGGGTGGCTGGAACCATGATTTTGGTGATTTTCGCCATCATCACTTACTACACCTCTACTTTGCTCGCCGATTGCTACAGAGCGCCGGACCCCATCACCAGAACACGCAACTACACGTACATGGGCGTCGTTCGAGCTTACCTTGGTGGTAAAAAGGTGCAGCTATGTGGACTAGCACAGTACGGCAACCTCGTTGGGGTCTCTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATTGGGAAAGCAAATTGTTTTCATGGTAAGGGACATGGTGCGAAATGTACCGCATCGAATTATCCATACATGGGGGCATTTGGCGGCCTCCAGATTCTTCTAAGTCAGATTCCTAATTTTCACAAGCTATCTTTCCTCTCAATCATTGCCGCGTTATGTCCTTCTCTTATGCATCTATTGGTATCGGTCTGGCCATCGCCAAAGTGGCAAGTGGGAAGGTTGGTAAGACAACACTGACAGGTACGGTGATAGGAGTGGACGTATCTGCGTCTGATAAAGTGTGGAAAGTGTTTCAAGCGGTTGGGGATATTGCGTTTTCGTACGCTTACACCACTATTCTCATTGAGATCCAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAGAAAGCAAGTCTTATTGGAGTCTCAACCACAACTGTTTTCTACCTCTTATGTGGTTGCATTGGGTATGCTGCATTCGGAAACATAGCCCCTGGSTGACTTCCTTACCGACTTTGGGTTTTACGAACCTTTCTGGCTCGTCATTTTCGCCAATGTTTGCATTGCTGTCCATTTAGTAGGTGCCTATCAGGTATACGTTCAGCCCTTTTTCCAATTTGTTGAGAGCAAATGCAACAAAAAGTGGCCTGAAAGCAATTTCATCAACAAAGAGTACTCGTTGAAGATACCATTGCTCGGAAAATTTCGTGTCAACCACTTCAGGCTGGTGTGGAGGACAAACTATGTGATTTTGACAACATTTATTGCAATGATATTC

AGTTTATTTTCCTGTGGCAATGCACATTGCTCAGACAAAGGTTAAGAAGTATTCGGGTAGATGGTTGGCGCTGAACCTCCTCGTGTTGGTTTGCTTGATTGTCTCCGCCTTAGCGGCAGTGGGATCCATTGTTGGCCTAATCAATAATGTCAAGAAATACAAGCCTTTCGAGAGTATAGACTAASEQ ID NO: 125: XP_013723586 AAP1-like X1 [Brassica napus] (protein)MKSFNTDQHGHSAAESADVYAMSDPTKNVDDDGREKRTGTWLTASAHIITAVIGSGVLSLAWAIAQLGWIAGTLILIIFSFITYFTSTMLADCYRAPDPLTGKRNYTYMDVVRSYLGGRKVQLCGVAQYGNLIGITVGYTITASISLVAIGKANCYHNKGHHADCTISNYPYMAAFGIIQILLSQIPNFHKLSFLSLMAAVMSFAYASIGIGLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATVLIEIQDTLRSSPAENKAMKRASFVGVSTTTFFYILCGCLGYAAFGNKAPGDFLTDFGFYEPFWLIDFANACIAFHLIGAYQVKPNPKGEKDCFLFALSRSLANEKETYFLQVFAQPIFQFVEKKCNRNWPDNKFITSEYSVNIPFLGKFSIN

WIGLKMLCWVCLIVSLLAAAGSIAGLISSVKTYKPFRTIHESEQ ID NO: 126: XP_013723586 AAP1-like X1 [Brassica napus] (genomic):Brara.I01660 | A09:11198108..11202102 forward

GATTGAAAAATAGACACTTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAAATGACAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGTTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTTAAAAACAAATG

ATCAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGT

SEQ ID NO: 127: XP_013723587 AAP1-like isoform X2 [Brassica napus] (protein)MKSFNTDQHGHSAAESADVYAMSDPTKNVDDDGREKRTGTWLTASAHIITAVIGSGVLSLAWAIAQLGWIAGTLILIIFSFITYFTSTMLADCYRAPDPLTGKRNYTYMDVVRSYLGGRKVQLCGVAQYGNLIGITVGYTITASISLVAIGKANCYHNKGHHADCTISNYPYMAAFGIIQILLSQIPNFHKLSFLSLMAAVMSFAYASIGIGLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATVLIEIQDTLRSSPAENKAMKRASFVGVSTTTFFYILCGCLGYAAFGNKAPGDFLTDFGFYEPFWLIDFANACIAFHLIGAYQVFAQPIFQFVEKKCNRNWPDNKFITSEYSVNIPFLGKFSINLFRLVWRTAYVVITTLVAMIFPFFNAILGLIG

TYKPFRTIHESEQ ID NO: 128: XP_013723587 AAP1-like isoform X2 [Brassica napus](genomic): Brara.I01660 | A09:11198108..11202102 forward

GATTGAAAAATAGACACTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAAATGACAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGTTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTTAAAAACAA

ATCAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGT

SEQ ID NO: 129: XP_013748815 AAP1-like [Brassica napus] (protein)MKGFNTEQDHPAAESGNVYDVSDPTKNVDDDGREKRTGTWLTASAHIITAVIGSGVLSLAWAIAQLGWIAGTLILVIFSFITYFTSTMLADCYRAPDPVTGKRNYTYMDVVRSYLGGRKVQLCGVAQYGNLIGITVGYTITASSISLVAVGKANCFHKKGHEADCTISNYPYMAVFGIIQIILSQIPNFHKLSFLSLMAAVMSFTYATIGIGLAIATVAGGKVGKTNMTGTVVGVDVTAAQKIWRSFQAVGDIAFAYAYATVLIEIQDTLKSSPAENKSMKRASLVGVSTTTFFYILCGCLGYAAGFNKAPGDFLTDFGFYEPFWLIDFANACIAFHLIGAYQVFAQPIFQFVEKRCNRNWWPDNKFITSEYSVNVPFLGKFNISLFRLVWRTAYVVITTVVAMIFPFFNAILGLIG

TYKPFRTIHESEQ ID NO: 130: XP_013748815 AAP1-like [Brassica napus] (genomic):Brara.I01660 | A09:11198108..11202102 forward

GATTGAAAAATAGACACTTTTTAGACCAACCCTTTTTTGAAAAGGGTTCTATACCAAAAATTCAATTTTGTTAAGTAATTTTTAAGGCATGGTTGGTTCCTTTTCCAAAAAAAAAGAGAGAGGATAACATAAGGTTCTCTTTCATTAAAATAATATACTGATTTTGTCTGGTATTTAGTTAGGCTGATCCTTGTCTGGAAAACTATTGTGGTCAGAATAGGAAAATAATTATTAGTATTAAAAATTTATACTAATATTAATTAAAAATGACAAAATATATATTAGTAATAAAAACATATAATATAATTTATAAGCGACAGTAACTTTTAGGTCAATTTGAAATAAAACTGGTTATGTAATGAGTTTATATAGAACAATGATGGTGGTTTATAAAATAGTTGATGTTACGAACTATAAGATCAATCATAAGAAATCATCATTGATATCTTTTGAAACAATCGAAAAGCTTATGCATCCGATGAGTTGTGGTTAGGAATGTAGATAAAGTAATGGATTTATAGATTTTCTAACATTTCCTGCCAATTTGGTTTTACAGAAGAAAAAAAATCTATCGGGCATATAAATTACTGTTGCGTATAAATTGATTTTTTTTGTAGACGCTTATAAATTGATATATATCCTTTTAAATATTTAAATTTAACTGAATATAAAAACAGTGGTAACCGTTCTTTTAATTTTCTAGTTAGAAAAAATGTTGTTGAAATAATTAAAGGCTTGGTTATTATTTATACATGGATTATATTCGTAAGCAATTTAAAGTTTGTTGTCTCTAGTTTAATAAACGATGATTTTTTACGAATTCTTTTTATTAAGTAAAAACACTGAGTTATTGACAAAAAAAAAGAGTAAAACAACTGAGTTCAATGTAAAGTGTGGTAACCGTCCTTTTAATTTTCTAATGATAAACTATGGTTGTTGAAAAATTAATTAGACTTCGCTATTATTTTCATAAGGATTACATACTTCAAATAATTTAGAGTATGTTGTCTAGTTCAATAAACAATGAGGTTTTACTTCAAATCATTTTAAGTAAAACATTGAACTGAACGCAAAAGTGTGGTAACCGTCCTTTAATTTCCAACTATACAACGGTTGTTGAAATAATTAATTAGGCTGAAATTTTTCTTAAATAACTCACGGTTTTGTACTGTTTATAAGATCCAAAACTGTGCAACCCGAGGAAAACAGAAATTAAATAATAGTTTGACATATGAGGCAAAGGGTTTTGGTAGTATTAGCATTATTGTTGGTATTTGATGTTGATGGAGTATTATATATACTAGTATAGTTCCGTGGAGTGTTTTATGTTTGATGTATGAAACAGAAGATTAATTAATAGCTTAAAAACAAATG

ATGTGATTTTACTTTATGACCGTTACCAAATGTACAGTTTAGTTATCTACCATATTG

ATCAATTTTACTATTGAGTCTTGTTTGCTTTGAATATGAACCTCTTAATAGAATTGTGGTTTGAAATTATTGACAATGACAATCAATTCTCTATGGACCACTTTTAATACAAAGGGTAAAAAGAAGAGAATCTGTCTTTTAGCTTAAAGGTATAACATGTGCTTATTAGTGACAAGATGTCACATTCAAAGACAGCAAACAATGATATCAATGGACTTTAGCTTAATGAGTTGACAATATAGTTAAAATTTTGTTGTCTCTTAATGATATTAGCGTTCACCTTTCTAGTGTACATGCATTTAGTTCAATAGAGTGTATATGTCGACTAGAAAGTGACGGCTTAATAAGATTTAAGTTAAACACATGAGACAAAACTGGATTTGCACACACTAACCGGT

BARLEYSEQ ID NO: 131: BAJ85485 [Hordeum vulgare subsp. vulgare] (protein)MGMEKSKANPAAFSIAEAGFGDRTDIDDDGRERRTGTLVTASAHIITAVIGSGVLSLAWAIAQLGWVIGPAVLVVAFSVITWWFCSSLLADCYRSPDPVHGKRNYTYGQAVRANLGVSKYRLCSLAQYLNLVGVTIGYTITTAISMGAIGRSNCFHRNGHNAACEASNTTNMIIFAAIQILLSQLPNFHKVWWLSIVAAVMSLAYSSIGLGLSIAKIAGGVHAKTTLTGVTVGVDVSASEKIWWRTFQSLGDIAFAYSYSNVLIEIQDTLRSSPAENTVMKKASLIGVSTTTTFYMLCGVLGYAAFGSSAPGNFLTGFGFYEPFWLVDVGNVCIVVHLVGAYQVFCQPFYQFVEGWARSRWPDSAFLHAERVVQLPAIVGGGEFPVSPFRLVWRTAYVALTAVVAMLFPFFN

VQGLVKDVAGYKPFKVSSEQ ID NO: 132: BAJ85485 [Hordeum vulgare subsp. vulgare] (genomic)ATGGGGATGGAGAAGAGCAAGGCTAACCCTGCCGCCTTCAGCATCGCTGAGGCCGGCTTTGGAGACCGGACGGACATCGACGACGACGGCCCGCGAGAGGCGTACCGGTACGCTGGTGACGGCCAGCGCACACATCATCACGGCGGTGATCGGGTCCGGGGTGCTGTCGCTGGCGTGGGCCATCGCACAGCTCGGGTGGGTCATCGGCCCCGCGCGTGCTCGTCGCCTTCTCCGTCATCACCTGGTTCTGCTCCAGCCTACTGGCCGACTGCTATCGCTCGCCGGACCCCGTCCACGGCAAGCGCAACTACACCTACGGCCAAGCCGTCAGGGCAAACCTAGGAGTTAGCAAGTACAGGCTCTGCTCACTGGCCCAATACCTCAACTTGGTTGGCGTGACCATTGGCTACACCATCACCACGGCCATCAGCATGGGGGCGATCGGACGGTCCAATTGCTTCCACCGCAATGGCCACAATGCGGCCTGCGAGGCATCCAACACCACCAACATGATTATATTTGCTGCCATCCAAATCTTGCTCTCGCAGCTCCCCAACTTCCACAAGGTCTGGTGGCTCTCCATTGTTGCTGCCGTCATGTCCCTCGCCTACTCGTCCATTGGTCTCGGCCTCTCCATAGCAAAAATCGCAGGTGGGGTGCATGCCAAGACAACGCTAACAGGGGTGACCGTTGGGGTGGATGTATCTGCGAGTGAGAAAATTTGGAGAACGTTCCAGTCTCTTGGGGACATCGCCTTTGCATACTCCTACTCCAATGTTCTCATCGAAATCCAGGACACGCTGCGGTCGAGCCCGGCGGAGAACACGGTGATGAAGAAGGCATCCTTGATCGGCGTTTCCACGACCACCACGTTCTACATGCTGTGCGGGGTGCTGGGCTACGCGGCGTTCGGCAGCAGCGCCCCGGGTAACTTCCTCACGGGCTTCGGCTTCTACGAGCCCTTCTGGCTCGTCGACGTCGGCAACGTCTGCATCGTCGTGCACCTCGTCGGCGCCTACCAGGTCTTCTGCCAGCCCTTCTACCAGTTCGTCGAGGGCTGGGCGCGCTCCCGGTGGCCCGACAGCGCCTTCCTCCACGCCGAGCGAGTCGTGCAACTCCCGGCCATTGTCGGCGGCGGCGAGTTCCCCGTGAGCCCATTTCGCCTGGTCTGGCGAACGGCGTACGTGGCCCTCACGGCGGTGGTGGCCATGTTGTTCCCCTTCTTCAACGACTTTCTTGGCCTCATCGGCCGC

GCCAAGGTGCGGCGGTTCTCGCCGACGTGGACGTGGATGAACGTGCTTAGCATCGCCTGCCTTGTCGTCTCTGTCCTCGCAGCCGCTGGTTCGGTGCAGGGGCTCGTCAAGGACGTGGCAGGGTACAAGCCATTCAAGGTCTCCTAASEQ ID NO: 133: BAJ91439.1 predicted protein [Hordeum vulgare subsp. vulgare] (protein)MTKDVEMAARNGSKGAAAGEAYYPSPPGQGGDVDVDDDGKQRRTGTVWTASAHIITAVIGSGVLSLAWATAQLGWVVGPVTLMLFAAITYYTSGLLADCYRTGDPLTGKRNYTYMDAVASYLSRWQVWACGVFQYVNLVGTAIGYTITASISAAAINKANCFHKNGRAADCGVYDSMYMVVFGVVQIFFSQVPNFHDLWWWLSILAAVMSFTYASIAVGLSLAQTISGPTGKSTLTGTEVGVDVDSAQKIWLAFQALGDIAFAYSYSMILIEIQDTVRSPPAENKTMKKATLVGVSTTTAFYMLCGCLGYAAFGNGAKGNILTGFGFYEPYWLIDFANVCIVVHLVGAYQVFCQPIFAAVENFAAATWWPNAGFITREHRVAAGKRLGFNLNLFRLTWRTAFVMV

VSLAAAVASIEGVTESLKNYVPFKTKSSEQ ID NO: 134: BAJ1439.1 predicted protein [Hordeum vulgare subsp. vulgare] (genomic)ATGGGGGAGAACGGTGTGGTGGCGAGCAAGCTGTGCTACCCGGCGGCGGCCATGGAGGTGGTCGCCGCCGAGCTCGGCCACACGGCCGGCTCCAAGCTGTACGACGACGACGGCCGCCTCAAGCGCACCGGGACGATGTGGACGGCGAGCGCGCACATCATCACGGCGGTGATCGGCTCCGGCGTGCTGTCGCTGGGGTGGGCGATCGCGCAGCTGGGTTGGGTGGCCGGCCCCGCCGTCATGCTGCTCTTCTCGTTCGTCACCTACTACACCTCCGCGCTGCTCGCCGACTGCTACCGCTCCGGCGACGAGAGCACCGGCAAGCGCAACTACACCTACATGGACGCCGTGAACGCCAACCTGAGTGGCATCAAGGTCCAGGTCTGCGGGTTCCTGCAGTACGCCAACATCGTCGGCGTCGCCATCGGCTACACCATTGCCGCCTCCATTAGCATGCTGGCGATCAAGCGGGCGAACTGCTTCCACGTCGAGGGGCACGGCGACCCGTGCAACATCTCGAGCACGCCGTACATGATCATCTTCGGCGTGGCGGAGATCTTCTTCTCGCAGATCCCGGACTTCGACCAGATCTCGTGGCTGTCCATCCTCGCCGCCGTCATGTCGTTCACCTACTCCACCATCGGGCTCGGCCTCGGCGTCGTGCAGGTGGTGGCCAACGGCGGCGTCAAGGGGAGCCTCACCGGGATCAGCATCGGCGTGGTGACGCCCATGGACAAGGTGTGGCGGAGCCTGCAGGCGTTCGGCGACATCGCCTTCGCCTACTCCTACTCCCTCATCCTCATCGAGATCCAGGACACCATCCGGGCGCCGCCGCCGTCGGAGTCGAGGGTGATGCGGCGCGCCACCGTGGTGAGCGTCGCCGTCACCACGCTCTTCTACATGCTCTGCGGCTGCACGGGGTACGCGGCGTTCGGCGACGCCGCGCCGGGCAACCTCCTCACCGGGTTCGGCTTCTACGAGCCCTTCTGGCTCCTCGACGTTGCCAACGCCGCCATCGTCGTCCACCTCGTCGGCGCCTACCAGGTCTACTGCCAGCCGCTGTTCGCCTTCGTCGAGAAGTGGGCGCAGCAGCGGTGGCCGAAATCATGGTACATCACCAAGGATATCGACGTGCCGCTCTCCCTCTCCGGCGGCGGCGGCGGCGGCGGAAGGTGCTACAAGCTGAACCTGTTCAGGCTGACATGGAGGTCGGCGTTCGTGGTGGCGACGACGGTGG

GGTTCTGGCCGCTCACCGTCTACTTCCCGGTGGAGATGTACATCGTGCAGAAGAGGATACCGAGGTGGAGCACGCGGTGGGTGTGCCTGCAGCTGCTCAGCCTCGCCTGCCTCGCCATCACCGTCGCCTCCGCCGCCGGCTCCATCGCCGGAATCCTCTCCGACCTCAAGGTCTACAAGCCGTTCGCCACCACCTACTAA WHEATSEQ ID NO: 135: EMS56484 [Triticum urartu] (protein)MEVVTALTNVEVPATGTVAEATDRSDAERASKWARCWRILGWWTLGEGIVGEDFGWSWGGGAGGCFYFPYFTCGQGSGDDDCVRGGAWGRGFGAGASPMTTAFHSAARGGAGGGLGQVAPAILSPDMPVALGLGVGHLSEGHGSPQPPAPVTLVDPLRDSARGFTREEVVAFGGIPDPVSAGRWMSARIQELPEVDDMQQRCAMREAKLHDAEISTGYFSSHGSDPFVVATHSDGGQRAFGYWIYPLGDASQLEAMGMEKGKADPATFSIAEAGFGDRTDIDDDGRERRTGTLVTASAHIITAVIGSGVLSLAQAIAQLGWVIGPAVLVAFSVITWFCSSLLADCYRSPDPVHGKRNYTYGQAVRANLGVSKYRLCSLAQYVNLVGVTIGYTITTAISMGAIGRSNCFHRNGHNAACEASNTTNMIIFAAIQILLSQLPNFHKIWWLSIVAAVMSLAYSSIGLGLSIAKIAGGVHAKTALTGVTVGVDVSASEKIWRTFQSLGDIAFAYSYSNVLIEIQDTLRSSPAENKVMKKASLIGVSTTTTFYMLCGVLGYAAFGSSAPGNFLTGFGFYEPFWLVDIGNVCIIVHLVGAYQVFCQPIYQFVEGWARSRWPDSAFLHAERVLRLPAVLG

QAKVRRFSPTWTWMNVLSVACLVVSVLAAAGSVQGLIKDVAGYKPFKVSSEQ ID NO: 136: EMS56484 [Triticum urartu] (genomic)ATGGAGGTGGTGACGGCCTTGACCAATGTTGAGGTTCCTGCGACTGGGACTGTGGCTGAGGCTACCGACAGGTCTGATGCTGAGAGGGCGTCCAAGTGGGCGCGGTGCTGGCGGATCCTTGGCTGGACGCTTGGTGAGGGCATCGTCGGCGAGGACTTTGGATGGAGTTGGGGAGGTGGAGCTGGTGGTTGCTTCTATTTCCCTTACTTCACATGTGGTCAAGGCTCCGGAGATGATGACTGCGTCCGAGGTGGGGCTTCCCCCAGGGGGTTCGGGGCAGGTGCGTCTCCTATGACGACGGCGTTCCACTCTGCTGCGAGGGGTGGGGCGGGAGGAGGGCTCGGGCAGGTGGCCCCCGCCATCCTCTCTCCCGACATGCCCGTGGCCCTGGGCCTCGGTGTGGGGCACTTGTCCGAGGGGCATGGGAGCCCGCAGCCGCCTGCTCCGGTAACCTTGGTTGACCCTTTGCGGGATTCAGCGCGAGGCTTTACTAGGGAGGAGGTCGTTGCTTTTGGCGGGATTCCTGACCCGGTCTCGGCGGGGAGATGGATGAGTGCTCGCATTCAGGAGCTTCCGGAGGTTGATGACATGCAGCAGAGGTGCGCTATGAGGGAGGCCAAGCTTCATGATGCTGAGATCTCTACTGGTTATTTTTCGAGCCACGGCAGTGATCCGTTCGTGGTCGCTACTCACTCCGATGGAGGCCAGAGAGCATTTGGTTACTGGATCTATCCGCTGGGAGACGCTAGCCAGCTAGAAGCAATGGGGATGGAGAAGGGCAAGGCTGACCCTGCCACCTTCAGCATCGCTGAGGCCGGCTTTGGAGACCGGACGGACATCGACGACGACGGACGCGAGAGGCGTACCGGTACGCTGGTGACGGCGAGCGCCCACATCATCACGGCGGTCATCGGGTCCGGGGGTGCTGTCGCTGGCGTGGGCCATCGCGCAGCTCGGGTGGGTCATCGGCCCCGCCGTGCTCGTCGCCTTCTCCGTCATCACCTGGTTCTGCTCCAGCCTACTGGCCGACTGCTACCGCTCACCGGACCCCGTCCACGGCAAGCGCAACTACACCTACGGCCAGGCCGTCAGGGCCAACCTAGGAGTTAGCAAATACAGGCTCTGCTCTCTGGCCCAATACGTCAACTTGGTTGGCGTCACCATTGGCTACACCATCACCACGGCCATCAGCATGGGGGGCGATCGGACGGTCGAATTGCTTCCACCGCAATGGCCACAATGCGGCCTGCGAGGCATCCAACACCACCAACATGATTATATTTGCTGCCATCCAAATCTTGCTCTCGCAACTCCCCAACTTCCACAAGATCTGGTGGCTCTCCATTGTTGCCGCCGTCATGTCCCTCGCCTACTCCTCCATTGGTCTCGGCCTCTCCATAGCAAAAATCGCAGGTGGGGTGCATGCCAAGACAGCGCTAACAGGGGTGACCGTTGGGGTGGATGTATCCGCGAGTGAGAAAATTTGGAGGACGTTCCAGTCTCTTGGGGACATCGCCTTTGCATACTCCTACTCCAATGTGCTCATCGAAATCCAGGACACGCTGCGGTCGAGCCCGGCAGAGAACAAGGTGATGAAGAAGGCGTCCTTGATCGGTGTTTCCACGACCACCACGTTCTACATGCTGTGCGGGGTGCTGGGCTACGCGGCGTTCGGCAGCAGCGCCCCCGGGTAACTTCCTCACGGGCTTCGGCTTCTACGAGCCCTTCTGGCTCGTCGACATCGGCAACGTTTGCATCATCGTGCACCTCGTTGGCGCCTACCAGGTC

GACAGCGCCTTCCTCCATGCCGAGCGCGTGTTGCGCCTTCCGGCCGTTCTCGGAGGCGGAGAGTTCCCGGTTAGCCCGTTACGCCTGGTCTGGCGAACGGCGTACGTGGTTCTCACGGCGGTGGTGGCCATGCTGTTCCCCTTCTTCAACGACTTCCTTGGCCTCATTGGCGCCGTCTCGTTTTGGCCGCTCACCGTCTACTTCCCCGTTGAGATGTACATGGCACAAGCCAAAGTGCGCCGGTTCTCGCCGACGTGGACGTGGATGAACGTGCTTAGCGTCGCGTGCCTTGTCGTCTCTGTCCTCGCCGCAGCTGGTTCTGTGCAGGGGCTCATCAAGGACGTCGCAGGGTACAAGCCATTCAAGGTCTCCTAASEQ ID NO: 137: EMS68703.1 TRIUR3_33957 [Triticum urartu] (protein)MGVLGLIQLVGRRRGEYPLVRDTVTPQGGGESGGGGGGAMDVDGHLPRTHGDVDDDGRERRTGTVWTAAAHIITAVIGSGVLSLAWAMAQLGWVAGPLTLVLFAIITFYTCGLLADRYRVGDPVTGKRNYTYTEAVQAYLGTCSPQARPFLLIKMQPEMMCMCSGGWHVWFCGFCQYVNMFGTGIGYTITASTSAAALKKSNCFHWHGHKADCSQYLSAYIIAFGVVQVIFCQVPNFHKLSWLSIVAAIMSFSYATIAVGLSLAQTISGPRGRTSLTGTEVGVDVDASQKVWMTFQALGNVAFAYSYSIILIEIQDTLRSPPGENKTMRKATLMGISTTTAFYMLCGCLGYSAFGNDANGNILTGFGFYEPYWLVDFANVCIVLHLVGGFQVFCQPLFAAMYI

SEQ ID NO: 138: EMS68703.1 TRIUR3_33957 [Triticum urartu] (genomic)ATGGGGGTCCTCGGCCTGATCCAACTGGTCGGGAGACGACGTGGTGAGTACCCCCTAGTCCGGGACACCGTCACCCCCCAGGGAGGCGGCGAGAGCGGCGGCGGCGGAGGCGGGGCCATGGACGTCGACGGCCACCTTCCCCGCACCCACGGCGACGTCGACGACGACGGCAGGGAGAGGAGAACAGGGACGGTATGGACGGCGGCGGCGCACATCATAACGGCGGTGATCGGGTCCGGCGTGCTGTCGCTGGCCTGGGCCATGGCGCAGCTGGGCTGGGTGGCCGGGCCGCTCACCCTGGTGCTCTTCGCCATCATCACCTTCTACACCTGCGGCCTCCTCGCCGACCGCTACCGCGTCGGCGACCCCGTCACGGGCAAGCGCAACTACACCTACACCGAGGCCGTCCAGGCCTACCTAGGTACGTGCTCGCCTCAAGCTCGCCCGTTTTTACTCATCAAAATGCAACCTGAGATGATGTGCATGTGTTCAGGCGGGTGGCACGTCTGGTTCTGCGGCTTCTGCCAGTACGTCAACATGTTCGGCACCGGCATCGGCTACACCATCACCGCCTCCACCAGCGCCGCGGCCTTGAAGAAGTCCAACTGCTTCCACTGGCACGGGCACAAGGCGGACTGCAGCCAGTACCTGAGCGCCTACATCATCGCCTTCGGGGTGGTGCAGGTCATCTTCTGCCAGGTGCCCAACTTCCACAAGCTCTCGTGGCTCTCCATCGTCGCCGCCATCATGTCCTTCTCCTACGCCACCATCGCCGTCGGCCTCTCGCTGGCGCAGACCATCTCGGGGGCCCAGGGGGAGGACGTCGCTGACCGGCACGGAGGTCGGGGTGGACGTCGACGCCTCGCAGAAGGTCTGGATGACGTTCCAGGCCCTCGGCAACGTCGCCTTCGCCTACTCCTACTCCATAATCCTCATCGAGATCCAGGACACGCTGCGGTCACCTCCGGGCGAGAACAAGACGATGCGGAAGGCGACGCTGATGGGCATCTCGACGACGACGGCCTTCTACATGCTGTGCGGCTGCCTGGGCTACTCGGCCTTCGGCAACGACGCCAACGGCAACATCCTGACGGGGTTCGGCTTCTACGAGCCCTACTGGCTGGTGGACTTCGCCAACGTCTGCATCGTGCTCCACCTGGTGGGCGGCTTCCAGGTCTTCTGCCAGCCGCTGTTCGCGGCGATGTACATCCGGCAGCGGCAGATCCCGCGGTTCGGCACCAAGTGGGTGGCGCTGCAGAGCCTCAGCTTCGTCTGCTTCCTCGTCACCGTCGCCGCCTGCGCCGCCTCCATCCAGGGCGTCCGCGACTCGCTCAAGACCTACACGC

BRASSICA RAPA SEQ ID NO: 139: VDC65345.1 unnamed protein product [Brassica rapa] (protein)MSPSPPLTMKSLDTLHNPSAVESGNAAVKNVDDDGREKRTGTFTASAHIITAVIGSGVLSLAWALAQLGWVAGTMILVIFAIITYYTSTLLADCYRAPDPITGTRNYTYMGVVRAYLGGKKVQLCGLAQYGNLVGVSIGYTITASISLVAIGRANCFHDKGHGAKCTASNYPYMVAFGGLQILLSQIPNFHKLSFLSIIAAVMSFFSYASIGIGLAIAKVASGKVGKTTLTGTVIGVDVSASDKVWKAFQAVGDIAFSYAYTTILIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNLSPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKKWPESNFINKEYSLLKIPLLGKFRVNFFRLVWRTNYVILTTFIAMIFPFFNSILGLL

KKYKPFESID SEQ ID NO: 140: VDCT65345.1 unnamed protein product [Brassica rapa] (genomic)ATGTCTCCTTCTCCCCCTCTTACAATGAAATCCTTGGACACACTCCACAATCCCTCGGCGGTTGAGTCCGGTAACGCCGCTGTGAAGAACGTCGACGATGATGGTCGAGAGAAGAGAACGGGGACGTTTCTGACGGCGAGTGCGCACATTATCACGGCGGTGATAGGCTCAGGAGTGTTGTCTTTGGCTTGGGCATTAGCACAGCTTGGTTGGGTGGCTGGAACCATGATTTTGGTGATTTTCGCCATCATCACTTACTACACGTCTACTTTGCTCGCCGATTGCTACAGAGCGCCGGACCCCATCACCGGAACACGCAACTACACGTACATGGGCGTCGTTCGAGCTTACCTTGGTGGTAAAAAGGTGCAGCTATGTGGACTAGCACAGTACGGAAACCTCGTTGGGGTCTCTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATTGGGAGAGCAAATTGTTTTCATGACAAGGGACATGGTGCGAAATGTACCGCATCGAATTATCCATACATGGTGGCATTTGGCGGCCTCCAGATTCTTCTAAGTCAGATTCCTAATTTTCACAAGCTATCTTTCCTCTCAATCATTGCCGCGGTTATGTCCTTCTCTTATGCATCTATTGGTATCGGTCTGGCCATCGCCAAAGTAGCAAGTGGGAAGGTTGGTAAGACAACACTGACAGGTACGGTGATAGGAGTGGACGTATCTGCGTCTGATAAAGTGTGGAAAGCGTTTCAAGCGGTTGGGGATATTGCGTTTTCGTACGCTTACACCACTATTCTCATTGAGATACAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAGAAAGCAAGTCTTATTGGAGTCTCAACCACAACTGTTTTCTACCTCTTATGTGGTTGCATTGGATATGCTGCATTCGGAAACTTATCCCCTGGTGACTTCCTACCGACTTTGGGTTTTACGAACCTTTCTGGCTCGTCATTTTCGCCAATGTTTGCATTGCTGTCCATTTAGTAGGTGCCTATCAGGTATATGTTCAGCCTTTTTTCCAGTTTGTTGAGAGCAAATGTAACAAAAAGTGGCCTGAAAGCAATTTCATCAACAAAGAATACTCGTTGAAGATACCATTGCTCGGAAAATTTCGTGTCAACTTCTTCAGGCTGGTGTGGAGGACAAACTATGTGATTTTGACAACATTTATTGCAATGATATTCCCCTTCTTCAACTCCAT

CAATGCACATTGCTCAGACAAAGGTTAAGAAGTATTCGGGTAGATGGTTGGCGCTGAACCTCCTCGTGCTGGTTTGCTTGATTGTCTCCGCCCTAGCTGCTGTGGGATCCATTGTTGGCCTAATCAATAATGTCAAGAAATACAAGCCTTTCGAGAGTATAGACTAASEQ ID NO: 141: RID57273.1 hypothetical protein BRARA_F00660 [Brassica rapa] (protein)MSPSPPLTMKSLDTLHNPSAVESGNAAVKNVDDDGREKRTGTFLTASAHIITAVIGSGVLSLAWALAQLGWVAGTMILVIFAIITYYTSTLLADCYRAPDPITGTRNYTYMGVVRAYLGGKKVQLCGLAQYGNLVGVSIGYTITASISLVAIGRANCFHDKGHGAKCTASNYPYMVAFGGLQILLSQIPNFHKLSFLSIIAAVMSFSYASIGIGLAIAKVASGKVGKTTLTGTVIGVDVSASDKVWKAFQAVGDIAFSYSYTTILIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNLSPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKKWPESNFINKEYSLKIPLLGKFRVNFFRLVWRTNYVILTTFIAMIFPFFNSILGLL

KKYKPFESID SEQ ID NO: 142: RID57273.1 hypothetical protein BRARA_F00660 [Brassica rapa] (genomic)ATGAAATCCTTGGACACACTCCACAATCCCTCGGCGGTTGAGTCCGGTAACGCCGCTGTGAAGAACGTCGACGATGATGGTCGAGAGAAGAGAACGGGGACGTTTCTGACGGCGAGTGCGCACATTATCACGGCGGTGATAGGCTCAGGAGTGTTGTCTITTGGCTTGGGCATTAGCACAGCTTGGTTGGGTGGCTGGAACCATGATTTTGGTGATTTTTCGCCATCATCACTTACTACACGTCTACTTTGCTCGCCGATTGCTACAGAGCGCCGGACCCCATCACCGGAACACGCAACTACACGTACATGGGCGTCGTTCGAGCTTACCTTGGTGGTAAAAAGGTGCAGCTATGTGGACTAGCACAGTACGGAAACCTCGTTGGGGTCTCTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATTGGGAGAGCAAATTGTTTTCATGACAAGGGACATGGTGCGAAATGTACCGCATCGAATTATCCATACATGGTGGCATTTGGCGGCCTCCAGATTCTTCTAAGTCAGATTCCTAATTTTCACAAGCTATCTTTCCTCTCAATCATTGCCGCGGTTATGTCCTTCTCTTATGCATCTATTGGTATCGGTCTGGCCATCGCCAAAGTGGCAAGTGGGAAGGTTGGTAAGACAACACTGACAGGTACGGTGATAGGAGTGGACGTATCTGCGTCTGATAAAGTGTGGAAAGCGTTTCAAGCGGTTGGGGATATTGCGTTTTCGTACGCTTACACCACTATTCTCATTGAGATACAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAGAAAGCAAGTCTTATTGGAGTCTCAACCACAACTGTTTTCTACCTCTTATGTGGTTGCATTGGGTATGCTGCATTCGGAAACTTATCCCCTGGTGACTTCCTTACCGACTTTGGGTTTTACGAACCTTTCTGGCTCGTCATTTTCGCCAATGTTTGCATTGCTGTCCATTTAGTAGGTGCCTATCAGGTATATGTTCAGCCTTTTTTCCAGTTTGTTGAGAGCAAATGTAACAAAAAGTGGCCTGAAAGCAATTTCATCAACAAAGAATACTCGTTGAAGATACCATTGCTCGGAAAATTTCGTGTCAACTTCTTCAGGCTGGTGTGGAGGACAAACTATGTGATTTTGACAACATTTATTGCAATGATATTCCCCTTCTTCAACTCCATCTT

ATGCACATTGCTCAGACAAAGGTTAAGAAGTATTCGGGTAGATGGTTGGCGCTGAACCTCCTCGTGCTGGTTTGCTTGATTGTCTCCGCCCTAGCTGCTGTGGGATCCATTGTTGGCCTAATCAATAATGTCAAGAAATACAAGCCTTTCGAGAGTATAGACTAASEQ ID NO: 143: XP_009148321.1 AAP8 [Brassica rapa] (protein)MSPSPPLTMKSLDTLHNPSAVESGNAAVKNVDDDGREKRTGTFLTASAHIITAVIGSGVLSLAWALAQLGWVAGTMILVIFAIITYYTSTLLADCYRAPDPITGTRNYTYMGVVRAYLGGKKVQLCGRAQYGNLVGVSIGYTITASISLVAIGRANCFHDKGHGAKCTASNYPYMVAFGGLQILLSQIPNFHKLSFLSIIAAVMSFSYASIGIGLLAIAKVASGKVGKTTLLTGTVIGVDVSASDKVWKAFQAVGDIAFSYSYTTILIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNLLSPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKKWPESNFINKEYSLKIPLLGKFRVNFFRLVWRTNYVILTTFIAMIFPFFNSILGLL

KKYKPFESID SEQ ID NO: 144: XP_009148321 AAP8 [Brassica rapa] (genomic)ATGTCTCCTTCTCCCCCTCTTACAATGAAATCCTTGGACACACTCCACAATCCCTCGGCGGTTGAGTCCGGTAACGCCGCTGTGAAGAACGTCGACGATGATGGTCGAGAGAAGAGAACGGGGACGTTTCTGACGGCGAGTGCGCACATTATCACGGCGGTGATAGGCTCAGGATGTGTTGTCTTTGGCTTGGGCATTAGCACAGCTTGGTTGGGTGGCTGGAACCATGATTTTGGTGATTTTCGCCATCATCACTTACTACACGTCTACTTTGCTCGCCGATTGCTACAGAGCGCCGGACCCCATCACCGGAACACGCAACTACACGTACATGGGCGTCGTTCGAGCTTACCTTGGTGGTAAAAAGGTGCAGCTATGTGGACGAGCACAGTACGGAAACCTCGTTGGGGTCTCTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATTGGGAGAGCAAATTGTTTTCATGACAAGGGACcATGGTGCGAAATGTACCGCATCGAATTATCCATACATGGTGGCATTTGGCGGCCTCCAGATTCTTCTAAGTCAGATTCCTAATTTTCACAAGCTATCTTTCCTCTCAATCATTGCCGCGGTTATGTCCTTCTCTTATGCATCTATTGGTATCGGTCTGGCCATCGCCAAAGTGGCAAGTGGGAAGGTTGGTAAGACAACACTGACAGGTACGGTGATAGGAGTGGACGTATCTGCGTCTGATAAAGTGTGGAAAGCGTTTCAAGCGGTTGGGGATATTGCGTTTTCGTACGCTTACACCACTATTCTCATTGAGATACAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAGAAAGCAAGTCTTATTGGAGTCTCAACCACAACTGTTTTCTACCTCTTATGTGGTTGCATTGGGTATGCTGCATTCGGAAACTTATCCCCTGGTGACTTCCTTACCGACTTTGGGTTTTACGAACCTTTCTGGCTCGTCATTTTCGCCAATGTTTGCATTGCTGTCCATTTAGTAGGTGCCTATCAGGTATATGTTCAGCCTTTTTTCCAGTTTGTTGAGAGCAAATGTAACAAAAAGTGGCCTGAAAGCAATTTCATCAACAAAGAATACTCGTTGAAGATACCATTGCTCGGAAAATTTCGTGTCAACTTCTTCAGGCTGGTGTGGAGGACAAACTATGTGATTTTGACAACATTTATTGCAATGATA

AACAGTTTATTTTCCTGTGGCAATGCACATTGCTCAGACAAAGGTTAAGAAGTATTCGGGTAGATGGTTGGCGCTGAACCTCCTCGTGCTGGTTTGCTTGATTGTCTCCGCCCTAGCTGCTGTGGGATCCATTGTTGGCCTAATCAATAATGTCAAGAAATACAAGCCTTTCGAGAGTATAGACTAA SEQ ID NO: 145: RID48756.1 hypothetical protein BRARA_I015242 [Brassica rapa] (protein)MLLSLSSLPRFFSSKMKSYATEYNPSAVETAGNNFDDDGREKRTGTLMTATAHIITAVIGSGVLSLAWAIAQLGWVAGTVILVTFAVINYFTSTMLADCYRSPDTGIRNYNYMDVVRAYLGGWKVKLCGLAQYGSLVGITIGYTITASISLVAIGKANCFHDKGHDAKCSVSNYPLMAAFGITQIVLSQIHNFHKLSFLSIIATVMSFSYASIGIGLALAALASGKVGKTDLTGTVVGVDVTASDKIWRSFQAAGDIAFSYAFSVVLVEIQDTLRSSPPENKVMKKASLAGVSTTTGFYILCGCIGYAAFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIAAYQVFAQPIFQFIEKKCNKAWPESNFIAKDYSINIPLLGKCRINFFRLVWRSTYVILTTVVAMIFPFFNAIL

SVKAYKPFHNLDSEQ ID NO: 146: RID48756.1 hypothetical protein BRARA_I05242 [Brassica rapa] (genomic)ATGCTTTTATCACTTTCTTCTCTTCCTCGGTTTTTCTCGTCTAAAATGAAAAGCTACGCCACTGAGTATAATCCCTCGGCCGTGGAAACCGCCGGGAATAACTTCGACGATGATGGTCGGGAGAAGAGAACGGGGACGTTGATGACGGCGACCGCGCACATAATCACGGCGGTGATAGGTTCTGGAGTCTTGTCGTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGCAGGAACGGTGATTTTGGTAACTTTTGCCGTTATAAATTACTTCACATCTACAATGCTTGCGGACTGCTATCGATCTCCGGACACAGGAATACGTAATTATAATTACATGGACGTTGTCAGAGCTTACCTTGGTGGTTGGAAAGTGAAGCTGTGTGGACTGGCACAGTACGGGAGTCTAGTAGGGATCACTATTGGCTACACCATCACTGCCTCCATAAGCTTAGTAGCGATCGGGAAAGCAAATTGTTTTCATGACAAGGGACATGATGCAAAATGTTCCGTATCAAATTATCCACTCATGGCGGCGTTTGGTATCACCCAGATTGTTCTTAGTCAGATTCATAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCTACCGTTATGTCCTTCTCTTATGCATCCATCGGAATTGGCTTAGCCTTGGCTGCTCTGGCAAGTGGGAAGGTTGGTAAGACGGATCTGACGGGCACGGTGGTTGGAGTAGACGTAACTGCGTCTGACAAAATATGGAGGTCGTTTCAAGCAGCTGGAGACATTGCCTTTTCGTACGCATTTTCCGTTGTTCTCGTTGAGATTCAGGATACACTGAGATCAAGCCCACCAGAGAACAAAGTCATGAAAAAAGCAAGCCTTGCTGGAGTTTCAACTACAACTGGTTTCTACATCTTGTGTGGCTGCATCGGATATGCTGCTTTTGGAAACCAAGCCCCTGGAGACTTCCTAACTGACTTTGGTTTTTATGAGCCTTACTGGCTCATTGATTTTGCTAATGCTTGCATTGCTGTCCACCTAATCGCAGCCTATCAGGTGTTTGCACAACCAATATTCCAGTTTATTGAGAAGAAATGCAACAAAGCGTGGCCAGAAAGCAACTTCATCGCCAAAGATTATTCGATAAACATACCATTGCTAGGGAAATGTCGCATCAACTTCTTCAGTTGGTCTGGAGGTCAACCTATGTGATTTTGACAACAGTTGTAGCGATGAT

AACAGTTTACTTCCCAGTGGAGATGCACATCTCGCAGAGAAAGGTTAAGAAGTATACTATGAGATGGATAGGGTTGAAACTCCTTGTATTGGTTTGTTTGGTTGTTTCGCTCCTAGCTGCAGTAGGATCCATTGTCGGCTTGATAAGTAGTGTAAAGGCATACAAGCCTTTCCACAATTTAGATTAGSEQ ID NO: 147: XP_009118279.1 PREDICTED: amino acid permease 8-like[Brassica rapa] (protein)MLLSLSSLPRFFSSKMKSYATEYNPSAVETAGNNFDDDGREKRTGTLMTATAHIITAVIGSGVLSLAWAIAQLGWVAGTVILVTFAVINYFTSTMLADCYRSPDTGIRNYNYMDVVRAYLGGWKVKLCGLAQYGSLVGITIGYTITASISLVAIGKANCFHDKGHDAKCSVSNYPLMAAFGITQIVLSQIHNFHKLSFLSIIATVMSFSYASIGIGLALAALASGKVGKTDLTGTVVGVDVTASDKIWRSFQAAGDIAFSYAFSVVLVEIQDTLRSSPPENKVMKKASLAGVSTTTGFYILCGCIGYAAFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIAAYQFAQPIFQFIEKKCNKAWPESNFIAKDYSINIPLLGKCRINFFRLVWRSTYVILTTVVAMIFPFFNAIL

VKAYKPFHNLDSEQ ID NO: 148: XP_009118279.1 PREDICTED: amino acid permease 8-like[Brassica rapa] (genomic)ATGCTTTTATCACTTTCTTCTCTTCCTCGGTTTTTCTCGTCTAAAATGAAAAGCTACGCCACTGAGTATAATCCCTCGGCCGTGGAAACCGCCGGGAATAACTTCGACGATGATGGTCGGGAGAAGAGAACGGGGACGTTGATGACGGCGACCGCGCACATAATCACGGCGGTGATAGGTTCTGGAGTCTTGTCGTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGCAGGAACGGTGATTTTGGTAACTTTTGCCGTTATAAATTACTTCACATCTACAATGCTTGCGGACTGCTATCGATCTCCGGACACAGGAATACGTAATTATAATTACATGGACGTTGTCAGAGCTTACCTTGGTGGTTGGAAAGTGAAGCTGTGTGGACTGGCACAGTACGGGAGTCTAGTAGGGATCACTATTGGCTACACCATCACTGCCTCCATAAGCTTAGTAGCGATCGGGAAAGCAAATTGTTTTCATGACAAGGGACATGATGCAAAATGTTCCGTATCAAATTATCCACTCATGGCGGCGTTTGGTATCACCCAGATTGTTCTTAGTCAGATTCATAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCTACCGTTATGTCCTTCTCTTATGCATCCATCGGAATTGGCTTAGCCTTGGCTGCTCTGGCAAGTGGGAAGGTTGGTAAGACGGATCTGACGGGCACGGTGGTTGGAGTAGACGTAACTGCGTCTGACAAAATATGGAGGTCGTTTCAAGCAGCTGGAGACATTGCCTTTTCGTACGCATTTTCCGTTGTTCTCGTTGAGATTCAGGATACACTGAGATCAAGCCCACCAGAGAACAAAGTCATGAAAAAGCAAGCCTTGCTGGAGTTTCAACTACAACTGGTTTCTACATCTTGTGTGGCTGCATCGGATATGCTGCTTTTGGAAACCAAGCCCCTGGAGACTTCCTAACTGACTTTGGTTTTTATGAGCCTTACTGGCTCATTGATTTTGCTAATGCTTGCATTGCTGTCCACCTAATCGCAGCCTATCAGGTGTTTGCACAACCAATATTCCAGTTTATTGAGAAGAAATGCAACAAAGCGTGGCCAGAAAGCAACTTCATCGCCAAAGATTATTCGATAAACATACCATTGCTAGGGAAATGTCGCATCAACTTCTTCAGATTGGTCTGGAGGTCAACCTATGTGATTTTGACAACAGTTGTAGCGATGATA

AACAGTTTACTTCCCAGTGGAGATGCACATCTCGCAGAAAAAGATTAAGAAGTATACTATGAGATGGATAGGGTTGAAACTCCTTGTATTGGTTTGTTTGGTTGTTTCGCTCCTAGCTGCAGTAGGATCCATTGTCGGCTTGATAAGTAGTGTAAAGGCATACAAGCCTTTCCACAATTTAGATTAGSEQ ID NO: 149: RID48754.1 hypothetical protein BRARA_I05240 [Brassica rapa] (protein)MLLHISFLSSSVSSPLKMKSFDTSSVVESGAGAGNNVDDDCREKRTGTLITASAHIITTVIGSGVLSLAWAIAQLGWVVGTVILVAFAVIVNYTSRMLADSYRSPEGTRNYTYMDVVRVYLGGRKVQLCGLAQFGSLVGVTIGYTITASISLVAIGKANCFHDKGHGAKCSVSNYPLMAAFGIVQIFLSQIPNFHKLSFLSIIATVMSFSYASIGFGLALAALASGKVGKTGLTGTVVGVDVTASDKLWKSFQAAGNIAFSYAYSVVLVEIQDTLRSSPPENKVMKKASLAAVSTTTAFYILCGCIGYATFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIGAYQVFAQPIFQFVEKKCNQAWPESNFITKEHSMNIPLLGKCRINFFRLVWRTTYVIFSTVVAMIFPFFNAI

SVKAYKPFHNLDSEQ ID NO: 150: RID48754.1 hypothetical protein BRARA_I05240 [Brassica rapa] (genomic)ATGCTTTTGCATATCTCTTTTCTCTCTTCTTCAGTTTCTCCTCTCAAAATGAAAAGCTTCGACACGAGCTCAGTGGTTGAATCCGGTGCTGGCGCCGGGAATAACGTCGACGATGATTGTCGGGAGAAGAGAACGGGGACCTTGATAACGGCGAGTGCCCACATAATCACGACAGTGATAGGTTCTGGAGTCTTGTCGTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGTQGGAACAGTGATTTTGGTAGCCTTTGCCGTCATAGTTAATTACACATCCAGAATGCTCGCCGACAGTTATCGATCCCCGGAGGGAACACGCAACTATACTTACATGGACGTCGTCCGAGTCTACCTTGGTGGTAGGAAAGTGCAGCTGTGTGGACTAGCACAGTTCGGGAGTCTCGTAGGGGTTACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTGGCGATTGGGAAAGCAAATTGTTTTCATGACAAGGGACATGGTGCGAAATGTTCCGTATCAAATTATCCACTCATGGCGGCGTTTGGAATCGTCCAGATTTTTCTTAGTCAGATTCCTAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCCACCGTTATGTCCTTCTCTTATGCATCTATCGGTTTTGGCTTAGCCTTGGCCGCTCTGGCAAGTGGGAAGGTTGGTAAGACGGGACTGACAGGCACGGTGGTTGGAGTGGATGTAACTGCGTCTGACAAATTATGGAAGTCATTTCAAGCGGCTGGAAACATTGCCTTTTCATACGCTTATTCCGTTGTTCTCGTTGAGATTCAGGACACACTGAGATCAAGCCCACCAGAGAACAAAGTCATGAAAAAGCAAGCCTTGCTGCAGTCTCAACTACAACTGCTTTCTACATCTTATGTGGCTGCATCGGATATGCTACATTTGGAAACCAAGCCCCCGGAGACTTCCTTACTGACTTTGGTTTTTATGAACCTTACTGGCTCATCGATTTTGCTAATGCTTGCATCGCTGTCCACCTTATCGGAGCTTATCAGGTGTTTGCACAACCAATATTCCAGTTTGTTGAGAAGAAATGCAATCAGGCGTGGCCAGAAAGCAACTTCATCACCAAAGAACATTCGATGAACATACCGTTGCTTGGAAAATGTCGCATTAACTTCTTCAGACTGGTGTGGAGGACAACCTATGTGATTTTCTCAACAGTTGTAGCAATGATA

AACAGTTTACTTCCCGGTGGAGATGCACATCTCGCAGAAAAAGGTTAAGAAGTATTCTGTGAGATGGATAGTATTGAAACTCCTTGTTTTGGTTTGTTTAATTGTTTCGCTCCTAGCTGCCATAGGATCCATCGTTGGCTTGATAAGTAGTGTCAAGGCATACAAGCCTTTCCACAATTTAGATTAGSEQ ID NO: 151: XP_009118276.1 AAP8-like isoform X2 [Brassica rapa] (protein)MKSFDTSSVVESGAGAGNNVDDDCCREKRTGTLITASAHIITTVIGSGVLSLAWAIAQLGWVVGTVILVAFAVIVNYTSRMLADSYRSPEGTRNYTYMDVVRVYLGGRKVQLCGLAQFGSLVGVTIGYTITASISLVAIGKANCFHDKGHGAKCSVSNYPLMAAFGIVQIFLSQIPNFHKLSFLSIIATVMSFSYASIGFGLALAALASGKVGKTGLTGTVVGVDVTASDKLWKSFQAAGNIAFSYAYSVVLVEIQDTLRSSPPENKVMKKASLAAVSTTTAFYILCGCIGYATFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIGAYQVFAQPIFQFVEKKCNQAWP

TVYFPVEMHISQKKVKKYSVRWIVLKLLVLVCLIVSLLAAIGSIVGLISSVKAYKPFHNLDSEQ ID NO: 152: XP_009118276.1 AAP8-like isoform X2 [Brassica rapa] (genomic)ATGAAAAGCTTCGACACGAGCTCAGTGGTTGAATCCGGTGCTGGCGCCGGGAATAACGTCGACGATGATTGTCGGGAGAAGAGAACGGGGACCTTGATAACGGCGAGTGCCCACATAATCACGACAGTGATAGGTTCTGGAGTCTTGTCGTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGTAGGAACAGTGATTTTGGTAGCCTTTGCCGTCATAGTTAATTACACATCCAGAATGCTCGCCGACAGTTATCGATCCCCGGAGGGAACACGCAACTATACTTACATGGACGTCGTCCGAGTCTACCTTGGTGGTAGGAAAGTGCAGCTGTGTGGACTAGCACAGTTCGGGAGTCTCGTAGGGGTTACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTGGCGATTGGGAAAGCAAATTGTTTTCATGACAAGGGACATGGTGCGAAATGTTCCGTATCAAATTATCCACTCATGGCGGCGTTTGGAATCGTCCAGATTTTTCTTAGTCAGATTCCTAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCCACCGTTATGTCCTTCTCTTATGCATCTATCGGTTTTGGCTTAGCCTTGGCCGCTCTGGCAAGTGGGAAGGTTGGTAAGACGGGACTGACAGGCACGGTGGTTGGAGTGGATGTAACTGCGTCTGACAAATTATGGAAGTCATTTCAAGCGGCTGGAAACATTGCCTTTTCATACGCTTATTCCGTTGTTCTCGTTGAGATTCAGGACACACTGAGATCAAGCCCACCAGAGAACAAAGTCATGAAAAAAGCAAGCCTTGCTGCAGTCTCAACTACAACTGCTTTCTACATCTTATGTGGCTGCATCGGATATGCTACATTTGGAAACCAAGCCCCCCGGAGACTTCCTTACTGACTTTGGTTTTTATGAACCTTACTGGCTCATCGATTTTGCTAATGCTTGCATCGCTGTCCACCTTATCGGAGCTTATCAGGTGTTTGCACAACCAATATTCCAGTTTGTTGAGAAGAAATGCAATCAGGCGTGGCCAGAAAGCAACTTCATCACCAAAGAACATTCGATGAACATACCGTTGCTTGGAAAATGTCGCATTAACTTCTTCAGACTGGTGTGGAGGACAACCTATGTGATTTTCTCAACAGTT

GTTAAGAAGTATTCTGTGAGATGGATAGTATTGAAACTCCTTGTTTTGGTTTGTTTAATTGTTTCGCTCCTAGCTGCCATAGGATCCATCGTTGGCTTGATAAGTAGTGTCAAGGCATACAAGCCTTTCCACAATTTAGATTAGSEQ ID NO: 153: RID57272.1 hypotetical protein BRARA_F00659 [Brassica rapa] (protein)MSPSPPLTMKSLDTLHNPSAVESGNAAVKNVDDDGREKRTGTFLTASAHIITAVIGSGVLSLAWALAQLGWVAGTMILVIFAIITYYTSTLLADCYRAPDPITGTRNYTYMGVVRAYLGGKKVQLCGLAQYGNLVGVSIGYTITASISLVAIGRANCFHDKGHGAKCTASNYPYMVAFGGLQILLSQIPNFHKLSFLSIIAAVMSFSYASIGIGLAIAKVASGKVGKTTLTGTVIGVDVSASDKVWKAFQAVGDIAFSYAYTTILIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNLSPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKKWPESNFINKEYSLKIPLLGKFRVNFFRLVWRTNYVILTTFIAMIFPFFNSILGLL

KKYKPFESID SEQ ID NO: 154: RID57272.1 hypothetical protein BRARA_F00659 [Brassica rapa] (genomic)ATGAAAAGCTTTGACGCGGTGCATAATCCCTCTGCGGTGGAATCCGCTGACGCCAACGTCGACGATGATGGTCGGGAGAAGAGAACGGGGACGTTGATGACGGCGAGTGCGCACATAATCACGGCTGTGATAGGTTCCGGAGTGTTGTCGTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGCAGGAACATTGATTCTTGTAACTTTTGCCGTCGTCAATTACTACACATCCACTATGCTCGCCGATTGTTATAGATCGGACGCAGGAGCTCGCAACTATACGTACATGGACGTCGTTCGATCTTACCTTGGTGGTAGGAAAGTGCAGTTATGTGGACTGGCACAATACGGGTGTCTCGTAGGGGTCACTATTGGTTACACCATCACTGCGTCTATAAGTTTAGTAGCGATTTGGAAAGCAACTTGTTTTCATAAAAAAGGACATGGTGCAAAATGCTCCATCCCAAATTATCCATTCATGGTGGCCTTCGGGGTCGTGGAGATTCTTCTTAGTCAGCTTCCTAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCCGCCATTATGTCATTCTCTTATGCGTCTATCGGAATTGGTTTAGCCATTTCCGTTGTGGCAAGTGGAAAGGTTGGTAAGACGAGTGTGACGGGCACGGTGGTTGGAGTGGACGTGACCGCATCTGACAAAATATGGAAGGCGTTTCAAGCAACTGGAGACATTGCATTTTCATACTCTTTTTCCACTATTCTCGTTGAGATTCAGGATACATTGAGATCAAACCCACCAGAAAACAAAGTCATGAAAAAGCAACACTTGCCGGAGTCTCAACTACAACTGTTTTCTACATCTTATGTGGCTGCATGGGATATGCTGCATTTGGAAACCGAGCCCCCGGAGACTTCCTTACTGACTTTGGTTTTTATGAACCTTACTGGCTCATCAATTTTGCCAATGCTTGCATCGTCCTCCACCTAATTGCAGCCTATCAGGTGTTTGCACAACCAATTTTCCAACTTGTTGAGAACAAATGCAACAAAGCATGGCCAGAAAACAATTTCATCCACAAAGAACATTCGATAAACATACTATTCCTCGGAAAATGGCGCATCAACTTCTTCAGACTGGTGTGGAGGACAGCATATGTGATTTTGACAACATTTGTT

CTGGCCGCTAACAGTTTACTTCCCAGTGGAGATGCACATCTCGCAGAGAAAGGTTAAGAAGTATTCTATGAAATGGAATGCGTTGAAACTCCTTATATCGGTTTGTTTGATTGTTTCGCTCCTAGCTGCAATAGGATCCATTGTCGGCTTGATAAATAGTGTCAAGGCATACAAGCCTTTCCATAGTTAA BRASSICA OLERACEASEQ ID NO: 155: VDD42023.1 unnamed protein product [Brassica oleracea] (protein)MSPSPPPTMKSLDTLHNPSAVESGNAAVKNVDDDGREKRTGTFLTASAHIITAVIGSGVLSLAWALAQLGWVAGTMILVIFAIITYYTSTLLADCYRAPDPITGTRNYTYMGVVRAYLGGKKVQLCGLAQYGNLVGVSIGYTITASISLVAIGKANCFHGKGHGAKCTASNYPYMVAFGGLQILLSQIPNFHKLSFLSIIAAVMSFSYASIGIGLAIAKVASGKVGKTTLTGTVIGVDVSASDKVWKAFQAVGDIAFSYAYTTILIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNIAPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKKWPESNFINKEYSLKIPLLGKFRVNHFRLVWRTNYVILTTFIAMIFPFFNSILGLLG

YKPFESID SEQ ID NO: 156: VDD42023.1 unnamed protein product [Brassica oleracea] (genomic)ATGTCTCCCTCTCCCCCTCCTACAATGAAATCCTTGGACACACTCCACAATCCCTCGGCGGTTGAGTCCGGTAACGCCGCTGTGAAGAACGTCGACGATGATGGTCGAGAGAAGAGAACGGGGACGTTTCTGACGGCGAGTGCGCACATTATCACGGCGGTGATAGGCTCAGGAGTGTTGTCTTTGGCTTGGGCATTAGCACAGCTTGGTTGGGTGGCTGGAACCATGATTTTGGTGATTTTCGCCATCATTACTTACTACACCTCTACTTTGCTCGCCGATTGCTACAGAGCGCCGGACCCCATCACCGGAACACGCAACTACACGTACATGGGCGTCGTTCGAGCTTACCTTGGTGGTAAAAAGGTGCAGCTATGTGGACTAGCACAGTACGGCAACCTCGTTGGGGTCTCTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATTGGGAAAGCAAATTGTTTTCATGGTAAGGGACATGGTGCGAAATGTACCGCATCGAATTATCCATACATGGTGGCATTTGGCGGCCTCCAGATTCTTCTAAGTCAGATTCCTAATTTTCACAAGCTATCTTTCCTCTCAATCATTGCCGCGGTTATGTCCTTCTCTTATGCATCTATTGGTATCGGTCTGGCCATCGCCAAAGTGGCAAGTGGGAAGGTTGGTAAGACAACGCTGACAGGTACGGTGATAGGAGTGGACGTATCTGCGTCTGATAAAGTATGGAAAGCGTTTCAAGCGGTTGGGGATATTGCGTTTTCGTACGCTTACACCACTATTCTCATTGAGATCCAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAGAAAGCAAGTCTTATTGGAGTCTCAACCACAACTGTTTTCTACCTCTTATGTGGTTGCATTGGGTATGCTGCATTCGGAAACATAGCCCCTGGTGACTTCCTTACCGACTTTGGGTTTTACGAACCTTTCTGGCTCGTCATTTTCGCCAATGTTTGCATTGCTGTCCATTTAGTAGGTGCCTATCAGGTATATGTTCAGCCCTTTTTCCAATTTGTTGAGAGCAAATGCAACAAAAAGTGGCCTGAAAGCAATTTCATCAACAAAGAGTACTCGTTGAAGATACCATTGCTCGGAAAATTTCGTGTCAACCACTTCAGGCTGGTGTGGAGGACAAACTATGTGATTTTGACAACATTTATTGCAATGATATTCC

GTTTATTTTCCTGTGGCAATGCACATTTCTCAGACAAAGGTTAAGAAGTATTCGGGTAGATGGTTGGCGCTGAACCTCCTCGTGTTGGTTTGCTTGATTGTCTCCGCCTTAGCTGCAGTGGGATCCATTGTTGGTCTAATCAATAATGTCAAGAAATACAAGCCTTTCGAGAGTATAGACTAASEQ ID NO: 157: XP_013586575.1 PREDICTED: amino acid permease 8 [Brassica oleracea var. oleracea] (protein)MSPSPPPTMKSLDTLHNPSAVESGNAAVKNVDDDGREKRTGTFLTASAHIITAVIGSGVLSLAWALAQLGWVAGTMILVIFAIITYYTSTLLADCYRAPDPITGTRNYTYMGVVRAYLGGKKVQLCGLAQYGNLVGVSIGYTITASISLVAIGKANCFHGKGHGAKCTASNYPYMGAFGGLQILLSQIPNFHKLSFLSIIAAVMSFSYASIGIGLAIAKVASGKVGKTTLTGTVIGVDVSASDKVWKAFQAVGDIAFSYAYTTILIEIQDTLRSSPPENKVMKKASLIGVSTTTVFYLLCGCIGYAAFGNIAPGDFLTDFGFYEPFWLVIFANVCIAVHLVGAYQVYVQPFFQFVESKCNKKWPESNFINKEYSLKIPLLGKFRVNHFRLVWRTNYVILTTFIAMIFPFFNSILGLLG

YKPFESID SEQ ID NO: 158: XP_013586575.1 PREDICTED: amino acid permease 8 [Brassica oleracea var. oleracea] (genomic)ATGTCTCCCTCTCCCCCTCCTACAATGAAATCCTTGGACACACTCCACAATCCCTCGGCGGTTGAGTCCGGTAACGCCGCTGTGAAGAACGTCGACGATGATGGTCGAGAGAAGAGAACGGGGACGTTTCTGACGGCGAGTGCGCACATTATCACGGCGGTGATAGGCTCAGGAGTGTTGTCTTTGGCTTGGGCATTAGCACAGCTTGGTTGGGTGGCTGGAACCATGATTTTGGTGATTTTCGCCATCATTACTTACTACACCTCTACTTTGCTCGCCGATTGCTACAGAGCGCCGGACCCCATCACCGGAACACGCAACTACACGTACATGGGCGTCGTTCGAGCTTACCTTGGTGGTAAAAAGGTGCAGCTATGTGGACTAGCACAGTACGGCAACCTCGTTGGGGTCTCTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATTGGGAAAGCAAATTGTTTTCATGGTAAGGGACATGGTGCGAAATGTACCGCATCGAATTATCCATACATGGGGGCATTTGGCGGCCTCCAGATTCTTCTAAGTCAGATTCCTAATTTTCACAAGCTATCTTTCCTCTCAATCATTGCCGCGGTTATGTCCTTCTCTTATGCATCTATTGGTATCGGTCTGGCCATCGCCAAAGTGGCAAGTGGGAAGGTTGGTAAGACAACGCTGACAGGTACGGTGATAGGAGTGGACGTATCTGCGTCTGATAAAGTATGGAAAGCGTTTCAAGCGGTTGGGGATATTGCGTTTTCGTACGCTTACACCACTATTCTCATTGAGATCCAGGACACATTGAGATCAAGCCCACCAGAGAACAAAGTGATGAAGAAAGCAAGTCTTATTGGAGTCTCAACCACAACTGTTTTCTACCTCTTATGTGGTTGCATTGGGTATGCTGCATTCGGAAACATAGCCCCTGGTGACTTCCTTACCGACTTTGGGTTTTACGAACCTTTCTGGCTCGTCATTTTCGCCAATGTTTGCATTGCTGTCCATTTAGTAGGTGCCTATCAGGTATATGTTCAGCCCTTTTTCCAATTTGTTGAGAGCAAATGCAACAAAAAGTGGCCTGAAAGCAATTTCATCAACAAAGAGTACTCGTTGAAGATACCATTGCTCGGAAAATTTCGTGTCAACCACTTCAGGCTGGTGTGGAGGACAAACTATGTGATTTTGACAACATTTATTGCAATGATATTCC

GTTTATTTTCCTGTGGCAATGCACATTGCTCAGACAAAGGTTAAGAAGTATTCGGGTAGATGGTTGGCGCTGCACCTCCTCGTGTTGGTTTGCTTGATTGTCTCCGCCTTAGCTGCAGTGGGATCCATTGTTGGCCTAATCAATAATGTCAAGAAATACAAGCCTTTCGAGAGTATAGACTAASEQ ID NO: 159: XP_013599620.1 PREDICTED: amino acid permease 8-like[Brassica oleracea var. oleracea] (protein)MKSFHTEYNPSAVEAAGNNFDDDGREKRTGTVMTASAHIITAVIGSGVLSLAWAIAQLGWVAGTVILVTFAVINYFTSTMLADCYRSPDTGIRNYNYMDVVRAYLGGWKVKLCGLAQYGSLVGITIGYTITASISLVAIGKANCFHEKGHGAKCSVSNYPLMAAFGIIQIVLSQIHNFHKLSFLSIIATVMSFSYASIGIGLALAALASGKVGKTDLTGTVVDVDVTASDKIWRSFQAAGDIAFSYAFSVVLVEIQDTLRSSPPENKVMKKASLAGVSTTTGFYILCGCIGYAAFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIAAYQVFAQPIFQFIEKKCNKAWPESN

PVEMHISQKKVKKYTMRWIGLKLLVLVCLVVSLLAAVGLISSVKAYPKFHNLDSEQ ID NO: 160: XP_013599620.1 PREDICTED: amino acid permease 8-like[Brassica oleracea var. oleracea] (genomic)ATGAAAAGCTTCCACACTGAGTATAATCCCTCGGCCGTGGAAGCCGCCGGGAATAACTTCGACGACGATGGTCGGGAGAAGAGAACGGGGACGGTGATGACGGCAAGTGCTCACATTATCACTGCTGTGATAGGTTCCGGAGTCTTGTCCTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGCAGGAACAGTGATTTTGGTAACTTTTGCCGTTATAAATTACTTCACATCTACAATGCTTGCCGACTGTTATCGATCTCCGGACACAGGAATACGTAATTATAATTACATGGACGTTGTCAGAGCTTACCTTGGTGGTTGGAAAGTGAAGCTATGTGGTCTGGCACAGTACGGGAGTCTAGTAGGGATCACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTAGCGATAGGGAAAGCAAATTGTTTTCATGAAAAGGGACATGGTGCAAAATGTTCCGTATCGAATTATCCACTCATGGCGGCGTTTGGTATCATCCAGATTGTTCTTAGTCAGATTCATAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCCACCGTTATGTCCTTCTCTTATGCATCCATCGGAATTGGCTTGGCCTTGGCCGCTCTGGCAAGTGGGAAGGTTGGTAAGACGGATCTGACGGGCACGGTGGTTGGAGTAGACGTAACTGCGTCTGACAAAATATGGAGGTCGTTTCAAGCAGCTGGAGACATTGCCTTTTCGTACGCATTTTCCGTTGTTCTCGTTGAGATTCAGGATACACTGAGATCAAGCCCACCAGAGAACAAAGTCATGAAAAAAGCAAGCCTTGCTGGAGTTTCAACTACAACTGGTTTCTACATCTTATGTGGCTGCATCGGATATGCTGCTTTTGGAAACCAAGCCCCTGGAGACTTCCTAACTGACTTTGGTTTTTATGAGCCTTACTGGCTCATTGATTTTGCTAATGCTTGCATTGCTGTCCACCTAATCGCAGCCTATCAGGTGTTTGCACAACCAATATTCCAGTTTATTGAGAAGAAATGCAACAAAGCGTGGCCAGAAAGCAACTTTATCACCAAAGATTATTCGATAAACATACCATTGCTAGGGAAATGTCGCATCAACTTCTTCAGATTGGTCTGGAGGTCAACCTATGTGATTTTGACAACAGT

GGTTAAGAAGTATACTATGAGATGGATAGGGTTGAAACTCCTTGTATTGGTTTGTTTGGTTGTTTCGCTCCTAGCTGCAGTAGGATCCATTGTCGGCCTCATAAGTAGTGTAAAGGCATACAAGCCTTTCCACAATTTAGATTAGSEQ ID NO: 161: XP_013584691.1 PREDICTED: amino acid permease 8-like[Brassica oleracea var. oleracea] (protein)MKSFDAVHNPSAVESADANVDDDGREKRTGTLMTASAHIITAVIGSGVLSLAWAIAQLGWVAGTLILVTFAIVNYYSTMLADCYRSDAGARNYTYMDVVRSYLGGRKVQLCGLAQYGCLVGVTIGYTITASISLVAIWKATCFHKKGHGAKCSIPNYPFMAAFGVVEIFLSQLPNFHKLSFLSIIAAVMSFSYASIGIGLAIAVVASGKVGKTGVTGTVVGVDVTASDKIWKAFQATGDIAFSYSFSTILVEIQDTLRSSPPENKVMKKATLAGVSTTTVFYILCGCMGYAAFGNRAPGDFLTDFGFYEPYWLINFANACIVLHLIAAYQVFAQPIFQLVENKCNKAWPEN

FPVEMHISQRKVKKFSMKWNALKLLVLVCLIVSLLAAIGSIVGLINSVKAYKPFHSSEQ ID NO: 162 XP_013584691.1 PREDICTED: amino acid permease 8-like[Brassica oleracea var. oleracea] (genomic)ATGAAAAGCTTTGACGCGGTGCATAATCCCTCTGCGGTGGAATCCGCTGACGCCAACGTCGACGATGATGGTCGGGAGAAGAGAACGGGGACGTTGATGACGGCGAGTGCGCACATAATCACGGCGGTGATAGGTTCCGGAGTGTTGTCGTTGGCCTGGGCTATAGCACAGCTTGGTTGGGTGGCAGGAACACTGATTCTTGTAACTTTTGCCATCGTCAATTAACTACACATCCACTATGCTCGCCGACTGTTATAGATCGGACGCAGGAGCTCGCAACTATACGTACATGGACGTCGTCCGATCTTACCTTGGTGGTAGGAAAGTGCAGTTATGTGGACTGGCACAATACGGGTGTCTCGTAGGGGTCACTATTGGTTACACCATCACTGCCTCTATAAGTTTAGTAGCGATTTGGAAAGCAACTTGTTTTCATAAAAAAGGACATGGTGCGAAATGTTCCATCCCAAATTATCCATTCATGGCGGCCTTCGGGGTCGTGGAGATTTTTCTTAGTCAGCTTCCTAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCCGCCGTTATGTCATTCTCTTATGCGTCTATCGGAATTGGTTTAGCCATTGCCGTTGTGGCAAGTGGAAAGGTTGGTAAGACGGGTGTGACGGGCACGGTGGTTGGAGTGGACGTGACCGCATCTGACAAAATATGGAAGGCGTTTCAAGCAACTGGAGACATTGCATTTTCATACTCTTTTTCCACTATTCTCGTTGAGATTCAGGATACATTGAGATCAAGCCCACCAGAAAACAAAGTCATGAAAAAAGCAACACTCGCCGGAGTCTCAACTACAACTGTTTTCTACATCTTATGTGGCTGCATGGGATATGCTGCATTTGGAAACCGAGCCCCCGGAGACTTCCTTACTGACTTTGGTTTTTATGAACCTTACTGGCTCATCAACTTTGCCAATGCTTGCATCGTCCTCCACCTAATCGCAGCCTATCAGGTGTTTGCACAACCAATTTTCCAACTTGTTGAGAACAAATGCAACAAAGCATGGCCAGAAAACAATTTCATCAACAAAGAACATTCGATAAACATACCATTCCTCGGAAAATGGCGCATCAACTTCTTCAGACTGGTGTGGAGGACAGCATATGTGATTTTGACAACATTTG

TTCTGGCCGCTAACAGTTTACTTCCCAGTGGAGATGCACATCTCGCAGAGAAAGGTTAAGAAGTTTTCTATGAAATGGAATGCGTTGAAACTCCTTGTATTGGTTTGTTTGATTGTTTCGCTCCTAGCTGCAATAGGATCCATCGTCGGCTTGATAAATAGTGTCAAGGCATACAAGCCTTTCCATAGTTAASEQ ID NO: 163: XP_013601938.1 AAP8-like [Brassica oleracea var. oleracea] (protein)MLLHISFISSSVSPLKMKSFDTSSVVESGAGAGNNVDDDCREKRTGTLITASAHIITTVIGSGVLSLAQAIAQLGWVVGTVILVAFAVIVNYTSRMLADSYRSPEGTRNYTYMDVVRVYLGGRKVQLCGLAQFGSLVFVTIGYTITASISLVAIGKANCFHDKGHGADCSVSNYPLMAAFGIVQIFLSQIPNFHKLSFLSIIATVMSFSYASIGFGLALAALASGKVGKTGLTGTVVRVDVTASDKLWKSFQAAGNIAFSYAYSVVLVEIQDTLRSSPPENKVMKKASLAAVSTTTAFYILCGCIGYATFGNQAPGDFLTDFGFYEPYWLIDFANACIAVHLIGAYQVFAQPIFQFVEKKCNQAWPESNFITKEPSMNVPLLGKCRINFFRLVWRTTYVIFSTVVAMIFPFFNA

SVKAYKPFHNLD SEQ ID NO: 164: XP_013601938.1 AAP8-like [Brassica oleracea var. oleracea] (genomic)ATGCTTTTGCATATCTCTTTTATCTCTTCTTCAGTTTCTCCTCTCAAAATGAAAAGCTTCGACACGAGCTCAGTGGTTGAATCCGGTGCTGGCGCCGGGAATAACGTCGACGATGATTGTCGGGAGAAGAGAACGGGGACGTTGATAACGGCGAGTGCCCACATAATCACGACAGTGATAGGTTCTGGAGTCTTGTCGTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGTAGGAACAGTGATTTTGGTAGCCTTTGCCGTCATCGTTAATTACACATCCAGAATGCTCGCCGACAGTTATCGATCCCCGGAGGGAACACGCAACTATACTTACATGGACGTTGTCCGAGTCTACCTTGGTGGTAGGAAAGTGCAGCTATGTGGACTGGCACAGTTTGGGAGTCTCGTAGGGGTTACTATTGGTTACACCATCACTGCCTCCATAAGCTTAGTGGCGATTGGGAAAGCAAATTGTTTTCATGACAAGGGACATGGTGCGAAATGTTCCGTATCAAATTATCCACTCATGGCGGCGTTTGGGATCGTCCAGATTTTTCTTAGTCAGATTCCTAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCCACCGTTATGTCCTTCTCTTATGCATCTATCGGTTTTGGCTTAGCCTTGGCCGCTCTGGCAAGTGGGAAGGTTGGTAAGACGGGACTGACAGGCACGGTGGTTCGAGTGGACGTAACTGCGTCTGACAAATTATGGAAGTCATTTCAAGCGGCTGGAAACATTGCCTTTTCATACGCTTATTCCGTTGTTCTCGTTGAGATTCAGGACACACTGAGATCAAGCCCACCAGAGAACAAAGTCATGAAAAAAGCAAGCCTTGCTGCAGTCTCAACTACAACTGCTTTCTACATCTTATGTGGCTGCATCGGATATGCTACATTTGGAAACCAAGCCCCCGGAGACTTCCTTACTGACTTTGGTTTTTATGAACCTTACTGGCTCATCGATTTTGCTAATGCTTGCATCGCTGTCCACCTTATCGGAGCTTATCAGGTGTTTGCACAACCAATATTCCAGTTTGTTGAGAAGAAATGCAATCAGGCGTGGCCAGAAAGCAACTTCATCACCAAAGAACCTTCGATGAACGTACCGTTGCTTGGAAAATGTCGCATTAACTTCTTCAGACTGGTGTGGAGGACAACCTATGTGATTTTCTCAACAGTTGTAGCAATGAT

CTAACAGTTTACTTCCCGGTGGAGATGCACATCTCGCAGAAAAAGGTTAAGAAGTATTCGGTGAGATGGATAGTGTTGAAACTCCTTGTTTTGGTTTGTTTAATTGTTTCACTCCTAGCTGCCATAGGATCCATCGTTGGCTTGATAAGTAGTGTCAAGGCATACAAGCCTTTCCACAATTTAGATTAG BRASSICA CRETICASEQ ID NO: 165: RQL92522.1 hypothetical protein DY000_00018764 [Brassica cretical] (protein)MKTFHTEYSPSAVETAGNNFDDDGREKRTGTLMTATAHIITAVIGSGVLSLAWAIAQLGWVAGTVILVTFAVINYFTSTMLADCYRSPDTGIRNYNYMDVVRAYLGGWKVKLCGLAQYGSLVGITIGYTITASISLVAIGKANCFHEKGHGAKCSVSNYPLMAAFGIIQIVLSQIHNFHKLSFLSIIATVMSFSYASVGIGLALAALASGKVGKTDLTGTVVGVDVTASDKIWKSFQAAGDIAFSYAFSVDTLRSSPPENKVMKKASLAGVSTTTGFYILCGCIGYAAFGNQAPDFLTDFGFYEPYWLIDFANACIAVHLIAAYQVFAQPIFQFIEKKCNKAWPESNFITKD

HISQKKVKKYTMRWIGLKLLVLVCLVVSLLAAIGSIVGLISSVKAYKPFHNLDSEQ ID NO: 166: RQL92522. hypothetical protein DY000_00018764 [Brassica cretical] (genomic)ATGAAAACCTTCCACACTGAGTATAGTCCCTCGGCCGTGGAAACCGCCGGGAATAACTTCGACGATGATGGTCGGGAGAAGAGAACGGGGACGTTGATGACGGCGACCGCGCACATAATCACGGCGGTGATAGGTTCTGGAGTCTTGTCGTTGGCTTGGGCTATAGCACAACTTGGTTGGGTGGCAGGAACGGTGATTTTGGTAACTTTTGCCGTTATAAATTACTTCACATCTACAATGCTTGCCGACTGTTATCGATCCCCGGACACAGGAATACGTAATTATAATTACATGGACGTTGTCCGAGCTTACCTTGGTGGTTGGAAGGTAAAGTTATGTGGACTGGCACAGTACGGGAGTCTAGTAGGGATTACTATTGGTTATACCATCACTGCCTCCATAAGCTTAGTAGCGATCGGGAAAGCAAATTGTTTTCATGAAAAGGGACATGGTGCAAAATGTTCCGTATCAAATTATCCACTCATGGCGGCGTTTGGTATCATCCAGATTGTTCTTAGTCAAATTCATAATTTTCACAAGCTCTCTTTTCTCTCCATTATCGCCACGGTTATGTCCTTCTCTTATGCATCTGTCGGAATTGGCTTAGCCTTGGCCGCTCTGGCAAGTGGGAAGGTTGGTAAGACGGATCTGACGGGCACGGTGGTTGGAGTAGACGTAACTGCGTCTGACAAAATATGGAAGTCATTCCAAGCAGCTGGAGACATTGCCTTTTCGTATGCATTTTCCGTTGATACACTGAGATCAAGCCCACCAGAGAACAAAGTCATGAAAAAAGCAAGCCTTGCTGGAGTTTCAACTACAACTGGTTTCTACATCTTATGTGGCTGCATCGGATATGCTGCTTTTGGAAACCAAGCCCCTGGAGACTTCCTAACTGACTTTGGTTTTTATGAGCCTTACTGGCTCATTGATTTTGCTAATGCTTGCATTGCTGTCCACCTAATCGCAGCCTATCAGGTGTTTGCACAACCAATATTCCAGTTTATTGAGAAGAAATGCAACAAAGCGTGGCCAGAAAGCAACTTTATCACCAAAGATTATTCGATAAACATACCATTGCTAGGGAAATGTCGCATCAACTTCTTCAGATTGGTCTGGAGGTCAACCTATGTGATTTTGACAACAGTTGTAGCAATGATATTCCCCT

TTACTTCCCAGTGGAGATGCACATCTCGCAGAAAAAGGTTAAGAAGTATACTATGAGATGGATAGGGTTGAAACTCCTTGTATTGGTTTGTTTGGTTGTTTCGCTCCTAGCTGCCATAGGATCCATCGTTGGCTTGATAAGTAGTGTAAAGGCATACAAGCCTTTCCA CAATTTAGATTAGSEQ ID NO: 169: MUM4 promotergacggtggcattaagcatcttgcattgaatgatccgttatatataatctcaggttttttttgggttgaaatgatgatattaaattttaggttgacatgtacttatctttgtaatcaactaattaaatatttgaactgacatgtctacgttatatcataaataaaccaggtgttttaattaaataccacgattaaccttctaaaataaggaaaatcatattttattcgtcaatcactataatttggaaaacgatgcaatatatttatttctttctttatacacatacttaattaattatcaaaatttc

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1. A method of increasing seed yield in a plant, the method comprisingincreasing the activity of amino acid permease (AAP).
 2. (canceled) 3.The method of claim 1, wherein the method comprises increasing theexpression of AAP8, wherein the amino acid sequence of AAP8 comprises asequence as defined in SEQ ID NO: 2, 3 or 4 or a functional variant orhomologue thereof.
 4. The method of claim 3, wherein the methodcomprises introducing and expressing a nucleic acid construct, whereinthe construct comprises a nucleic sequence encoding an AAP8 polypeptideas defined in SEQ ID NO: 2, 3 or 4 or a functional variant or homologuethereof, wherein the nucleic acid sequence is operably linked to aregulatory sequence, wherein the regulatory sequence is a constitutiveor tissue-specific promoter, and wherein preferably the tissue-specificpromoter is a MUM4 promoter.
 5. (canceled)
 6. (canceled)
 7. (canceled)8. The method of claim 1, wherein the method comprises introducing atleast one mutation into the plant genome, wherein said mutationincreases the activity of an AAP polypeptide.
 9. (canceled) 10.(canceled)
 11. The method of claim 8, wherein the mutation is theinsertion of at least one additional copy of a nucleic acid sequenceencoding an AAP8 polypeptide or a homolog or functional variant thereof,such that the nucleic acid sequence is operably linked to a regulatorysequence, and wherein the mutation is introduced using targeted genomeediting and wherein preferably the nucleic acid sequence encodes an AAPpolypeptide as defined in SEQ ID NO: 2, 3 or 4 or a functional variantor homolog thereof; or wherein the method comprises or results inintroducing at least one mutation at position 410 of SEQ ID NO: 1 or ata homologous position in a homologous sequence.
 12. (canceled) 13.(canceled)
 14. The method of claim 1, wherein the plant is a crop plant,wherein preferably, the crop plant is selected from rice, maize, wheat,soybean, barley, cannabis and pennycress and Brassica.
 15. (canceled)16. A plant or plant progeny obtained or obtainable by the method of anyof claim
 1. 17. A genetically altered plant, part thereof or plantproduct, wherein the plant is characterized by an increase in seedyield, wherein the plant has increased activity of an AAP polypeptide.18. (canceled)
 19. The genetically altered plant of claim 17, whereinthe plant expresses a nucleic acid construct comprising a nucleic acidencoding an AAP8 polypeptide as defined in any of SEQ ID NO: 2, 3 or 4or a functional variant or homologue thereof.
 20. The geneticallyaltered plant of claim 17, wherein the plant has at least one mutationin its genome, wherein the mutation increases the activity of AAP8,wherein the mutation is in the insertion of at least one or moreadditional copy of a nucleic acid encoding an AAP8 polypeptide asdefined in SEQ ID NO: 2, 3 or 4 or homolog or functional variantthereof; or wherein the at least one mutation is at position 410 of SEQID NO: 1 or at a homologous position in a homologous sequence. 21.(canceled)
 22. (canceled)
 23. (canceled)
 24. The genetically alteredplant of claim 17, wherein the plant is a crop plant, preferably whereinthe crop plant is selected from rice, maize, wheat, soybean, barley,cannabis and pennycress and Brassica.
 25. (canceled)
 26. The geneticallyaltered plant of claim 17, wherein the plant part is a seed.
 27. Amethod of making a transgenic plant having an increase in seed yield,the method comprising introducing and expressing a nucleic acidconstruct comprising a nucleic acid sequence encoding an AAP8polypeptide as defined in SEQ ID NO: 2, 3 or 4 or a functional variantor homolog thereof.
 28. A method of making a genetically altered planthaving an increase in seed yield, the method comprising introducing amutation into the plant genome to increase the activity of an AAP8polypeptide, wherein the mutation is the insertion of one or moreadditional copies of a nucleic acid encoding an AAP8 polypeptide asdefined in SEQ ID NO: 2, 3 or 4 or a functional variant or homologthereof, such that the sequence is operably linked to a regulatorysequence; or wherein the method comprises or results in introducing atleast one mutation at position 410 of SEQ ID NO: 1 or at a homologousposition in a homologous sequence.
 29. (canceled)
 30. (canceled) 31.(canceled)
 32. (canceled)
 33. The method of claim 27, wherein the plantis a crop plant, wherein preferably the crop plant is selected fromrice, maize, wheat, soybean, barley, cannabis, pennycress and Brassica.34. (canceled)
 35. A method of screening a population of plants andidentifying and/or selecting a plant that has or will have increasedactivity of a AAP polypeptide, the method comprising detecting in theplant germplasm at least one polymorphism in the nucleic acid encodingan AAP polypeptide and selecting said plant or progeny thereof. 36.(canceled)
 37. The method of claim 35, wherein the polymorphism is asubstitution and wherein the substitution is at position 2635 of SEQ IDNO: 5 or a homologous substitution in a homologous sequence.
 38. Anucleic acid construct comprising a nucleic acid sequence encoding aAAP8 polypeptide as defined in SEQ ID NO: 2, 3 or 4 or a functionalvariant or homolog thereof.
 39. (canceled)
 40. (canceled)
 41. (canceled)42. (canceled)
 43. A method of producing a food or feed composition, themethod comprising a. producing a plant wherein the activity of an AAPpolypeptide is increased using the method defined in claim 27; b.obtaining a seed from said plant; and c. producing a food or feedcomposition from said seed.
 44. A method of increasing free amino acidand/or protein content in a plant, preferably increasing free amino acidand/or protein content in the seed or grain of said plant, the methodcomprising increasing the activity of amino acid permease (AAP),wherein, the method comprises increasing the activity and/or expressionof AAP8, wherein the amino acid sequence of AAP8 comprises a sequence asdefined in SEQ ID NO: 2, 3 or 4 or a functional variant or homologuethereof.
 45. (canceled)